ID   ADH_DROER               Reviewed;         256 AA.
AC   P28483; B3N5C6;
DT   01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 2.
DT   03-AUG-2022, entry version 113.
DE   RecName: Full=Alcohol dehydrogenase;
DE            EC=1.1.1.1;
GN   Name=Adh; ORFNames=GG25120;
OS   Drosophila erecta (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7220;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Gif-sur-Yvette stock 154.1;
RX   PubMed=8170369; DOI=10.1093/oxfordjournals.molbev.a040110;
RA   Jeffs P.S., Holmes E.C., Ashburner M.;
RT   "The molecular evolution of the alcohol dehydrogenase and alcohol
RT   dehydrogenase-related genes in the Drosophila melanogaster species
RT   subgroup.";
RL   Mol. Biol. Evol. 11:287-304(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tucson 14021-0224.01;
RX   PubMed=17994087; DOI=10.1038/nature06341;
RG   Drosophila 12 genomes consortium;
RT   "Evolution of genes and genomes on the Drosophila phylogeny.";
RL   Nature 450:203-218(2007).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a primary alcohol + NAD(+) = an aldehyde + H(+) + NADH;
CC         Xref=Rhea:RHEA:10736, ChEBI:CHEBI:15378, ChEBI:CHEBI:15734,
CC         ChEBI:CHEBI:17478, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.1;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10001};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a secondary alcohol + NAD(+) = a ketone + H(+) + NADH;
CC         Xref=Rhea:RHEA:10740, ChEBI:CHEBI:15378, ChEBI:CHEBI:17087,
CC         ChEBI:CHEBI:35681, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.1;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10001};
CC   -!- SUBUNIT: Homodimer.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC       family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; X54116; CAA38057.1; -; Genomic_DNA.
DR   EMBL; CH954177; EDV57956.1; -; Genomic_DNA.
DR   RefSeq; XP_001968897.1; XM_001968861.2.
DR   AlphaFoldDB; P28483; -.
DR   SMR; P28483; -.
DR   STRING; 7220.FBpp0143666; -.
DR   EnsemblMetazoa; FBtr0145174; FBpp0143666; FBgn0012261.
DR   GeneID; 6540581; -.
DR   KEGG; der:6540581; -.
DR   eggNOG; KOG4169; Eukaryota.
DR   HOGENOM; CLU_010194_2_16_1; -.
DR   OMA; TTLQCAQ; -.
DR   OrthoDB; 1053465at2759; -.
DR   PhylomeDB; P28483; -.
DR   ChiTaRS; Adh; fly.
DR   Proteomes; UP000008711; Unassembled WGS sequence.
DR   GO; GO:0005829; C:cytosol; IEA:EnsemblMetazoa.
DR   GO; GO:0032991; C:protein-containing complex; IEA:EnsemblMetazoa.
DR   GO; GO:0008774; F:acetaldehyde dehydrogenase (acetylating) activity; IEA:EnsemblMetazoa.
DR   GO; GO:0004022; F:alcohol dehydrogenase (NAD+) activity; ISS:UniProtKB.
DR   GO; GO:0042803; F:protein homodimerization activity; IEA:EnsemblMetazoa.
DR   GO; GO:0006117; P:acetaldehyde metabolic process; IEA:EnsemblMetazoa.
DR   GO; GO:0046164; P:alcohol catabolic process; IEA:EnsemblMetazoa.
DR   GO; GO:0048149; P:behavioral response to ethanol; IEA:EnsemblMetazoa.
DR   GO; GO:0006069; P:ethanol oxidation; IEA:EnsemblMetazoa.
DR   GO; GO:0006734; P:NADH metabolic process; IEA:EnsemblMetazoa.
DR   InterPro; IPR002425; ADH_Drosophila-type.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR020904; Sc_DH/Rdtase_CS.
DR   InterPro; IPR002347; SDR_fam.
DR   Pfam; PF00106; adh_short; 1.
DR   PRINTS; PR01168; ALCDHDRGNASE.
DR   PRINTS; PR00080; SDRFAMILY.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   PROSITE; PS00061; ADH_SHORT; 1.
PE   3: Inferred from homology;
KW   NAD; Oxidoreductase.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250"
FT   CHAIN           2..256
FT                   /note="Alcohol dehydrogenase"
FT                   /id="PRO_0000054460"
FT   ACT_SITE        153
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10001"
FT   BINDING         12..35
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
FT   BINDING         140
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   256 AA;  27723 MW;  686784D514099D8C CRC64;
     MAFTLTNKNV IFVAGLGGIG LDTSKELVKR DLKNLVILDR IENPAAIAEL KAINPKVTVT
     FYPYDVTVPI AETSKLLKTI FAKLTTVDVL INGAGILDDY QIERTIAVNY TGLVNTTTAI
     LDFWDKRKGG PGGIICNIGS VTGFNAIYQV PVYSGTKAAV VNFTSSLAKL APITGVTAYT
     VNPGITRTTL VHKFNSWLDV EPQVAEKLLA HPTQTSLSCA ENFVKAIELN ENGAIWKLDL
     GTLEAIQWSK HWDSGI