ADH_DROPE
ID ADH_DROPE Reviewed; 254 AA.
AC P37473; B4GK27; O16093; O16095; O16097; O18661; Q7KQD6; Q7KQD7; Q7KQD8;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Alcohol dehydrogenase;
DE EC=1.1.1.1;
GN Name=Adh; ORFNames=GL25993;
OS Drosophila persimilis (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7234;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2068088; DOI=10.1073/pnas.88.14.6097;
RA Schaeffer S.W., Miller E.L.;
RT "Nucleotide sequence analysis of Adh genes estimates the time of geographic
RT isolation of the Bogota population of Drosophila pseudoobscura.";
RL Proc. Natl. Acad. Sci. U.S.A. 88:6097-6101(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANTS LEU-82 AND LYS-185.
RC STRAIN=Persimi40, Persimi42, Persimi44, Persimi49, and Persimi50;
RX PubMed=9383055; DOI=10.1093/genetics/147.3.1091;
RA Wang R.L., Wakeley J., Hey J.;
RT "Gene flow and natural selection in the origin of Drosophila pseudoobscura
RT and close relatives.";
RL Genetics 147:1091-1106(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MSH-3 / Tucson 14011-0111.49;
RX PubMed=17994087; DOI=10.1038/nature06341;
RG Drosophila 12 genomes consortium;
RT "Evolution of genes and genomes on the Drosophila phylogeny.";
RL Nature 450:203-218(2007).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a primary alcohol + NAD(+) = an aldehyde + H(+) + NADH;
CC Xref=Rhea:RHEA:10736, ChEBI:CHEBI:15378, ChEBI:CHEBI:15734,
CC ChEBI:CHEBI:17478, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.1;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU10001};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a secondary alcohol + NAD(+) = a ketone + H(+) + NADH;
CC Xref=Rhea:RHEA:10740, ChEBI:CHEBI:15378, ChEBI:CHEBI:17087,
CC ChEBI:CHEBI:35681, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.1;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU10001};
CC -!- SUBUNIT: Homodimer.
CC -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB80702.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=AAB80704.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=AAB80708.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=AAB80710.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; M60997; AAA99035.1; -; Genomic_DNA.
DR EMBL; AF006564; AAB80702.1; ALT_FRAME; Genomic_DNA.
DR EMBL; AF006565; AAB80704.1; ALT_FRAME; Genomic_DNA.
DR EMBL; AF006566; AAB80706.1; -; Genomic_DNA.
DR EMBL; AF006567; AAB80708.1; ALT_FRAME; Genomic_DNA.
DR EMBL; AF006568; AAB80710.1; ALT_FRAME; Genomic_DNA.
DR EMBL; CH479184; EDW36993.1; -; Genomic_DNA.
DR PIR; S26784; S26784.
DR RefSeq; XP_002018797.1; XM_002018761.1.
DR AlphaFoldDB; P37473; -.
DR SMR; P37473; -.
DR STRING; 7234.FBpp0190100; -.
DR EnsemblMetazoa; FBtr0191608; FBpp0190100; FBgn0012635.
DR GeneID; 6593284; -.
DR KEGG; dpe:6593284; -.
DR eggNOG; KOG4169; Eukaryota.
DR HOGENOM; CLU_010194_2_16_1; -.
DR OMA; TTLQCAQ; -.
DR PhylomeDB; P37473; -.
DR ChiTaRS; Adh; fly.
DR Proteomes; UP000008744; Unassembled WGS sequence.
DR GO; GO:0005829; C:cytosol; IEA:EnsemblMetazoa.
DR GO; GO:0032991; C:protein-containing complex; IEA:EnsemblMetazoa.
DR GO; GO:0008774; F:acetaldehyde dehydrogenase (acetylating) activity; IEA:EnsemblMetazoa.
DR GO; GO:0004022; F:alcohol dehydrogenase (NAD+) activity; IEA:UniProtKB-EC.
DR GO; GO:0042803; F:protein homodimerization activity; IEA:EnsemblMetazoa.
DR GO; GO:0006117; P:acetaldehyde metabolic process; IEA:EnsemblMetazoa.
DR GO; GO:0046164; P:alcohol catabolic process; IEA:EnsemblMetazoa.
DR GO; GO:0048149; P:behavioral response to ethanol; IEA:EnsemblMetazoa.
DR GO; GO:0006069; P:ethanol oxidation; IEA:EnsemblMetazoa.
DR GO; GO:0006734; P:NADH metabolic process; IEA:EnsemblMetazoa.
DR InterPro; IPR002425; ADH_Drosophila-type.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR020904; Sc_DH/Rdtase_CS.
DR InterPro; IPR002347; SDR_fam.
DR Pfam; PF00106; adh_short; 1.
DR PRINTS; PR01168; ALCDHDRGNASE.
DR PRINTS; PR00080; SDRFAMILY.
DR SUPFAM; SSF51735; SSF51735; 1.
DR PROSITE; PS00061; ADH_SHORT; 1.
PE 3: Inferred from homology;
KW NAD; Oxidoreductase; Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250"
FT CHAIN 2..254
FT /note="Alcohol dehydrogenase"
FT /id="PRO_0000054488"
FT ACT_SITE 151
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10001"
FT BINDING 10..33
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 138
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT VARIANT 82
FT /note="V -> L (in strain: Persimi50)"
FT /evidence="ECO:0000269|PubMed:9383055"
FT VARIANT 185
FT /note="R -> K (in strain: Persimi44)"
FT /evidence="ECO:0000269|PubMed:9383055"
FT CONFLICT 16
FT /note="G -> A (in Ref. 2; AAB80702/AAB80704/AAB80706/
FT AAB80708/AAB80710)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 254 AA; 27645 MW; C76A72252371F0B6 CRC64;
MSLTNKNVVF VAGLGGIGLD TSRELVKRNL KNLVILDRID NPAAIAELKA INPKVTITFY
PYDVTVPVAE TTKLLKTIFA QVKTIDVLIN GAGILDDHQI ERTIAVNYTG LVNTTTAILD
FWDKRKGGPG GIICNIGSVT GFNAIYQVPV YSGSKAAVVN FTSSLAKLAP ITGVTAYTVN
PGITRTTLVH KFNSWLDVEP RVAEKLLEHP TQTSQQCAEN FVKAIELNKN GAIWKLDLGT
LEPITWTQHW DSGI
