ADH_DROPS
ID ADH_DROPS Reviewed; 254 AA.
AC Q6LCE4; P07158; Q27613; Q29P49; Q95092;
DT 01-APR-1988, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=Alcohol dehydrogenase;
DE EC=1.1.1.1;
GN Name=Adh; ORFNames=GA17214;
OS Drosophila pseudoobscura pseudoobscura (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=46245;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3666441; DOI=10.1093/genetics/117.1.61;
RA Schaeffer S.W., Aquadro C.F.;
RT "Nucleotide sequence of the Adh gene region of Drosophila pseudoobscura:
RT evolutionary change and evidence for an ancient gene duplication.";
RL Genetics 117:61-73(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2068088; DOI=10.1073/pnas.88.14.6097;
RA Schaeffer S.W., Miller E.L.;
RT "Nucleotide sequence analysis of Adh genes estimates the time of geographic
RT isolation of the Bogota population of Drosophila pseudoobscura.";
RL Proc. Natl. Acad. Sci. U.S.A. 88:6097-6101(1991).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=PS163, PS164, PS170, PS192, PS214, PS219, PS220, PS224, PS230,
RC PS243, PS245, PS261, PS262, PS265, PS274, PS279, PS281, PS282, PS283,
RC PS287, PS289, and PS297;
RX PubMed=1398051; DOI=10.1093/genetics/132.1.163;
RA Schaeffer S.W., Miller E.L.;
RT "Molecular population genetics of an electrophoretically monomorphic
RT protein in the alcohol dehydrogenase region of Drosophila pseudoobscura.";
RL Genetics 132:163-178(1992).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=BC414, BC86, BC93, BJ855, BN16, BR10F, BR3F, BR5F, BR7F, BR8F,
RC GB103, GB104, GB109, GB112, GB114, GB116, GB118, GB119, GB12, GB13, GB24,
RC GB26, GB27, GB32, GB33, GB41, GB54, GB59, GB66, GB68, GB69, GB78, GB82,
RC GB91, GB92, GB96, GC4, MT228, MT350, MV21, MV27, MV28, MV36, MV43, MXA31,
RC MXA64, MXZ26, OREG3, OREG4, PS101, PS102, PS108, PS126, PS139, SB14, SB18,
RC SB2, and SB6;
RX PubMed=1427038; DOI=10.1093/genetics/132.2.471;
RA Schaeffer S.W., Miller E.L.;
RT "Estimates of gene flow in Drosophila pseudoobscura determined from
RT nucleotide sequence analysis of the alcohol dehydrogenase region.";
RL Genetics 132:471-480(1992).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=PS298, PS299, PS302, PS306, PS309, PS314, PS315, and PS316;
RX PubMed=8244013; DOI=10.1093/genetics/135.2.541;
RA Schaeffer S.W., Miller E.L.;
RT "Estimates of linkage disequilibrium and the recombination parameter
RT determined from segregating nucleotide sites in the alcohol dehydrogenase
RT region of Drosophila pseudoobscura.";
RL Genetics 135:541-552(1993).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=GB48, PS228, PS231, PS232, PS236, PS242, PS246, PS248, PS251, PS254,
RC PS256, PS260, PS267, PS268, PS271, PS273, PS277, PS280, PS295, PS296,
RC PS301, PS308, PS311, PS317, PS320, PS322, PS323, PS329, PS331, PS333,
RC PS334, PS336, PS342, PS343, PS348, PS350, PS352, PS353, PS356, and PS367;
RX PubMed=12688655; DOI=10.1017/s0016672302005955;
RA Schaeffer S.W.;
RT "Molecular population genetics of sequence length diversity in the Adh
RT region of Drosophila pseudoobscura.";
RL Genet. Res. 80:163-175(2002).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MV2-25 / Tucson 14011-0121.94;
RX PubMed=15632085; DOI=10.1101/gr.3059305;
RA Richards S., Liu Y., Bettencourt B.R., Hradecky P., Letovsky S.,
RA Nielsen R., Thornton K., Hubisz M.J., Chen R., Meisel R.P., Couronne O.,
RA Hua S., Smith M.A., Zhang P., Liu J., Bussemaker H.J., van Batenburg M.F.,
RA Howells S.L., Scherer S.E., Sodergren E., Matthews B.B., Crosby M.A.,
RA Schroeder A.J., Ortiz-Barrientos D., Rives C.M., Metzker M.L., Muzny D.M.,
RA Scott G., Steffen D., Wheeler D.A., Worley K.C., Havlak P., Durbin K.J.,
RA Egan A., Gill R., Hume J., Morgan M.B., Miner G., Hamilton C., Huang Y.,
RA Waldron L., Verduzco D., Clerc-Blankenburg K.P., Dubchak I., Noor M.A.F.,
RA Anderson W., White K.P., Clark A.G., Schaeffer S.W., Gelbart W.M.,
RA Weinstock G.M., Gibbs R.A.;
RT "Comparative genome sequencing of Drosophila pseudoobscura: chromosomal,
RT gene, and cis-element evolution.";
RL Genome Res. 15:1-18(2005).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a primary alcohol + NAD(+) = an aldehyde + H(+) + NADH;
CC Xref=Rhea:RHEA:10736, ChEBI:CHEBI:15378, ChEBI:CHEBI:15734,
CC ChEBI:CHEBI:17478, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.1;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU10001};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a secondary alcohol + NAD(+) = a ketone + H(+) + NADH;
CC Xref=Rhea:RHEA:10740, ChEBI:CHEBI:15378, ChEBI:CHEBI:17087,
CC ChEBI:CHEBI:35681, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.1;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU10001};
CC -!- SUBUNIT: Homodimer.
CC -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=EAL34444.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; Y00602; CAA68645.1; -; Genomic_DNA.
DR EMBL; M60979; AAA99022.1; -; Genomic_DNA.
DR EMBL; M60980; AAA99023.1; -; Genomic_DNA.
DR EMBL; M60981; AAA99025.1; -; Genomic_DNA.
DR EMBL; M60982; AAA99016.1; -; Genomic_DNA.
DR EMBL; M60981; AAA99016.1; JOINED; Genomic_DNA.
DR EMBL; M60983; AAA99017.1; -; Genomic_DNA.
DR EMBL; M60984; AAA99018.1; -; Genomic_DNA.
DR EMBL; M60985; AAA99020.1; -; Genomic_DNA.
DR EMBL; M60986; AAA99019.1; -; Genomic_DNA.
DR EMBL; M60987; AAA99021.1; -; Genomic_DNA.
DR EMBL; M60988; AAA99024.1; -; Genomic_DNA.
DR EMBL; X64468; CAA45797.1; -; Genomic_DNA.
DR EMBL; X64469; CAA45798.1; -; Genomic_DNA.
DR EMBL; X64470; CAA45799.1; -; Genomic_DNA.
DR EMBL; X64471; CAA45800.1; -; Genomic_DNA.
DR EMBL; X64472; CAA45801.1; -; Genomic_DNA.
DR EMBL; X64473; CAA45802.1; -; Genomic_DNA.
DR EMBL; X64474; CAA45803.1; -; Genomic_DNA.
DR EMBL; X64475; CAA45804.1; -; Genomic_DNA.
DR EMBL; X64476; CAA45805.1; -; Genomic_DNA.
DR EMBL; X64477; CAA45806.1; -; Genomic_DNA.
DR EMBL; X64478; CAA45807.1; -; Genomic_DNA.
DR EMBL; X64479; CAA45808.1; -; Genomic_DNA.
DR EMBL; X64480; CAA45809.1; -; Genomic_DNA.
DR EMBL; X64481; CAA45810.1; -; Genomic_DNA.
DR EMBL; X64482; CAA45811.1; -; Genomic_DNA.
DR EMBL; X64483; CAA45812.1; -; Genomic_DNA.
DR EMBL; X64484; CAA45813.1; -; Genomic_DNA.
DR EMBL; X64485; CAA45814.1; -; Genomic_DNA.
DR EMBL; X64486; CAA45815.1; -; Genomic_DNA.
DR EMBL; X64487; CAA45816.1; -; Genomic_DNA.
DR EMBL; X64488; CAA45817.1; -; Genomic_DNA.
DR EMBL; X64489; CAA45818.1; -; Genomic_DNA.
DR EMBL; X62181; CAA44102.1; -; Genomic_DNA.
DR EMBL; X62182; CAA44103.1; -; Genomic_DNA.
DR EMBL; X62183; CAA44104.1; -; Genomic_DNA.
DR EMBL; X62184; CAA44105.1; -; Genomic_DNA.
DR EMBL; X62185; CAA44106.1; -; Genomic_DNA.
DR EMBL; X62186; CAA44107.1; -; Genomic_DNA.
DR EMBL; X62187; CAA44108.1; -; Genomic_DNA.
DR EMBL; X62188; CAA44109.1; -; Genomic_DNA.
DR EMBL; X62189; CAA44110.1; -; Genomic_DNA.
DR EMBL; X62190; CAA44111.1; -; Genomic_DNA.
DR EMBL; X62191; CAA44112.1; -; Genomic_DNA.
DR EMBL; X62192; CAA44113.1; -; Genomic_DNA.
DR EMBL; X62193; CAA44114.1; -; Genomic_DNA.
DR EMBL; X62194; CAA44115.1; -; Genomic_DNA.
DR EMBL; X62195; CAA44116.1; -; Genomic_DNA.
DR EMBL; X62196; CAA44117.1; -; Genomic_DNA.
DR EMBL; X62197; CAA44118.1; -; Genomic_DNA.
DR EMBL; X62198; CAA44119.1; -; Genomic_DNA.
DR EMBL; X62199; CAA44120.1; -; Genomic_DNA.
DR EMBL; X62200; CAA44121.1; -; Genomic_DNA.
DR EMBL; X62201; CAA44122.1; -; Genomic_DNA.
DR EMBL; X62202; CAA44123.1; -; Genomic_DNA.
DR EMBL; X62203; CAA44124.1; -; Genomic_DNA.
DR EMBL; X62204; CAA44125.1; -; Genomic_DNA.
DR EMBL; X62205; CAA44126.1; -; Genomic_DNA.
DR EMBL; X62206; CAA44127.1; -; Genomic_DNA.
DR EMBL; X62207; CAA44128.1; -; Genomic_DNA.
DR EMBL; X62208; CAA44129.1; -; Genomic_DNA.
DR EMBL; X62209; CAA44130.1; -; Genomic_DNA.
DR EMBL; X62210; CAA44131.1; -; Genomic_DNA.
DR EMBL; X62211; CAA44132.1; -; Genomic_DNA.
DR EMBL; X62212; CAA44133.1; -; Genomic_DNA.
DR EMBL; X62213; CAA44134.1; -; Genomic_DNA.
DR EMBL; X62214; CAA44135.1; -; Genomic_DNA.
DR EMBL; X62215; CAA44136.1; -; Genomic_DNA.
DR EMBL; X62216; CAA44137.1; -; Genomic_DNA.
DR EMBL; X62217; CAA44138.1; -; Genomic_DNA.
DR EMBL; X62218; CAA44139.1; -; Genomic_DNA.
DR EMBL; X62219; CAA44140.1; -; Genomic_DNA.
DR EMBL; X62220; CAA44141.1; -; Genomic_DNA.
DR EMBL; X62221; CAA44142.1; -; Genomic_DNA.
DR EMBL; X62222; CAA44143.1; -; Genomic_DNA.
DR EMBL; X62223; CAA44144.1; -; Genomic_DNA.
DR EMBL; X62224; CAA44145.1; -; Genomic_DNA.
DR EMBL; X62225; CAA44146.1; -; Genomic_DNA.
DR EMBL; X62226; CAA44147.1; -; Genomic_DNA.
DR EMBL; X62227; CAA44148.1; -; Genomic_DNA.
DR EMBL; X62228; CAA44149.1; -; Genomic_DNA.
DR EMBL; X62229; CAA44150.1; -; Genomic_DNA.
DR EMBL; X62230; CAA44151.1; -; Genomic_DNA.
DR EMBL; X62231; CAA44152.1; -; Genomic_DNA.
DR EMBL; X62232; CAA44153.1; -; Genomic_DNA.
DR EMBL; X62233; CAA44154.1; -; Genomic_DNA.
DR EMBL; X62234; CAA44155.1; -; Genomic_DNA.
DR EMBL; X62235; CAA44156.1; -; Genomic_DNA.
DR EMBL; X62236; CAA44157.1; -; Genomic_DNA.
DR EMBL; X62237; CAA44158.1; -; Genomic_DNA.
DR EMBL; X62238; CAA44159.1; -; Genomic_DNA.
DR EMBL; X68159; CAA48262.1; -; Genomic_DNA.
DR EMBL; X68160; CAA48263.1; -; Genomic_DNA.
DR EMBL; X68161; CAA48264.1; -; Genomic_DNA.
DR EMBL; X68162; CAA48265.1; -; Genomic_DNA.
DR EMBL; X68163; CAA48266.1; -; Genomic_DNA.
DR EMBL; X68164; CAA48267.1; -; Genomic_DNA.
DR EMBL; X68165; CAA48268.1; -; Genomic_DNA.
DR EMBL; X68166; CAA48269.1; -; Genomic_DNA.
DR EMBL; U64521; AAB06136.1; -; Genomic_DNA.
DR EMBL; U64522; AAB06137.1; -; Genomic_DNA.
DR EMBL; U64523; AAB06138.1; -; Genomic_DNA.
DR EMBL; U64524; AAB06139.1; -; Genomic_DNA.
DR EMBL; U64525; AAB06140.1; -; Genomic_DNA.
DR EMBL; U64526; AAB06141.1; -; Genomic_DNA.
DR EMBL; U64527; AAB06142.1; -; Genomic_DNA.
DR EMBL; U64528; AAB06143.1; -; Genomic_DNA.
DR EMBL; U64529; AAB06144.1; -; Genomic_DNA.
DR EMBL; U64530; AAB06145.1; -; Genomic_DNA.
DR EMBL; U64531; AAB06146.1; -; Genomic_DNA.
DR EMBL; U64532; AAB06147.1; -; Genomic_DNA.
DR EMBL; U64533; AAB06148.1; -; Genomic_DNA.
DR EMBL; U64534; AAB06149.1; -; Genomic_DNA.
DR EMBL; U64535; AAB06150.1; -; Genomic_DNA.
DR EMBL; U64536; AAB06151.1; -; Genomic_DNA.
DR EMBL; U64537; AAB06152.1; -; Genomic_DNA.
DR EMBL; U64538; AAB06153.1; -; Genomic_DNA.
DR EMBL; U64539; AAB06154.1; -; Genomic_DNA.
DR EMBL; U64540; AAB06155.1; -; Genomic_DNA.
DR EMBL; U64541; AAB06156.1; -; Genomic_DNA.
DR EMBL; U64542; AAB06157.1; -; Genomic_DNA.
DR EMBL; U64543; AAB06158.1; -; Genomic_DNA.
DR EMBL; U64544; AAB06159.1; -; Genomic_DNA.
DR EMBL; U64545; AAB06160.1; -; Genomic_DNA.
DR EMBL; U64546; AAB06161.1; -; Genomic_DNA.
DR EMBL; U64547; AAB06162.1; -; Genomic_DNA.
DR EMBL; U64548; AAB06163.1; -; Genomic_DNA.
DR EMBL; U64549; AAB06164.1; -; Genomic_DNA.
DR EMBL; U64550; AAB06165.1; -; Genomic_DNA.
DR EMBL; U64551; AAB06166.1; -; Genomic_DNA.
DR EMBL; U64552; AAB06167.1; -; Genomic_DNA.
DR EMBL; U64553; AAB06168.1; -; Genomic_DNA.
DR EMBL; U64554; AAB06169.1; -; Genomic_DNA.
DR EMBL; U64555; AAB06170.1; -; Genomic_DNA.
DR EMBL; U64556; AAB06171.1; -; Genomic_DNA.
DR EMBL; U64557; AAB06172.1; -; Genomic_DNA.
DR EMBL; U64558; AAB06173.1; -; Genomic_DNA.
DR EMBL; U64559; AAB06174.1; -; Genomic_DNA.
DR EMBL; U64560; AAB06175.1; -; Genomic_DNA.
DR EMBL; CH379058; EAL34444.1; ALT_SEQ; Genomic_DNA.
DR PIR; S06292; S06292.
DR PIR; S26769; S26769.
DR PIR; S26770; S26770.
DR PIR; S26779; S26779.
DR AlphaFoldDB; Q6LCE4; -.
DR SMR; Q6LCE4; -.
DR STRING; 7237.FBpp0278979; -.
DR eggNOG; KOG4169; Eukaryota.
DR HOGENOM; CLU_010194_2_16_1; -.
DR InParanoid; Q6LCE4; -.
DR OMA; TTLQCAQ; -.
DR PhylomeDB; Q6LCE4; -.
DR ChiTaRS; Adh; fly.
DR Proteomes; UP000001819; Genome assembly.
DR GO; GO:0004022; F:alcohol dehydrogenase (NAD+) activity; IEA:UniProtKB-EC.
DR GO; GO:0006066; P:alcohol metabolic process; IEA:InterPro.
DR InterPro; IPR002425; ADH_Drosophila-type.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR020904; Sc_DH/Rdtase_CS.
DR InterPro; IPR002347; SDR_fam.
DR Pfam; PF00106; adh_short; 1.
DR PRINTS; PR01168; ALCDHDRGNASE.
DR PRINTS; PR00080; SDRFAMILY.
DR SUPFAM; SSF51735; SSF51735; 1.
DR PROSITE; PS00061; ADH_SHORT; 1.
PE 3: Inferred from homology;
KW NAD; Oxidoreductase; Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250"
FT CHAIN 2..254
FT /note="Alcohol dehydrogenase"
FT /id="PRO_0000054491"
FT ACT_SITE 151
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10001"
FT BINDING 10..33
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 138
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT VARIANT 34
FT /note="V -> F (in strain: PS246)"
FT VARIANT 68
FT /note="V -> I (in strain: PS230)"
FT VARIANT 185
FT /note="K -> R (in strain: PS295)"
SQ SEQUENCE 254 AA; 27617 MW; D8484F252366D786 CRC64;
MSLTNKNVVF VAGLGGIGLD TSRELVKRNL KNLVILDRID NPAAIAELKA INPKVTITFY
PYDVTVPVAE TTKLLKTIFA QVKTIDVLIN GAGILDDHQI ERTIAVNYTG LVNTTTAILD
FWDKRKGGPG GIICNIGSVT GFNAIYQVPV YSGSKAAVVN FTSSLAKLAP ITGVTAYTVN
PGITKTTLVH KFNSWLDVEP RVAEKLLEHP TQTSQQCAEN FVKAIELNKN GAIWKLDLGT
LEPITWTQHW DSGI
