ID   ADH_DROSU               Reviewed;         254 AA.
AC   Q03384; Q27632;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   15-JUL-1999, sequence version 2.
DT   03-AUG-2022, entry version 94.
DE   RecName: Full=Alcohol dehydrogenase;
DE            EC=1.1.1.1;
GN   Name=Adh;
OS   Drosophila subobscura (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7241;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1560762; DOI=10.1093/oxfordjournals.molbev.a040718;
RA   Marfany G., Gonzalez-Duarte R.;
RT   "The Drosophila subobscura Adh genomic region contains valuable
RT   evolutionary markers.";
RL   Mol. Biol. Evol. 9:261-277(1992).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a primary alcohol + NAD(+) = an aldehyde + H(+) + NADH;
CC         Xref=Rhea:RHEA:10736, ChEBI:CHEBI:15378, ChEBI:CHEBI:15734,
CC         ChEBI:CHEBI:17478, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.1;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10001};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a secondary alcohol + NAD(+) = a ketone + H(+) + NADH;
CC         Xref=Rhea:RHEA:10740, ChEBI:CHEBI:15378, ChEBI:CHEBI:17087,
CC         ChEBI:CHEBI:35681, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.1;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10001};
CC   -!- SUBUNIT: Homodimer.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC       family. {ECO:0000305}.
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DR   EMBL; M55545; AAA28339.1; -; Genomic_DNA.
DR   PIR; A42180; A42180.
DR   AlphaFoldDB; Q03384; -.
DR   SMR; Q03384; -.
DR   FlyBase; FBgn0012930; Dsub\Adh.
DR   GO; GO:0004022; F:alcohol dehydrogenase (NAD+) activity; IEA:UniProtKB-EC.
DR   GO; GO:0006066; P:alcohol metabolic process; IEA:InterPro.
DR   InterPro; IPR002425; ADH_Drosophila-type.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR020904; Sc_DH/Rdtase_CS.
DR   InterPro; IPR002347; SDR_fam.
DR   Pfam; PF00106; adh_short; 1.
DR   PRINTS; PR01168; ALCDHDRGNASE.
DR   PRINTS; PR00080; SDRFAMILY.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   PROSITE; PS00061; ADH_SHORT; 1.
PE   3: Inferred from homology;
KW   NAD; Oxidoreductase.
FT   CHAIN           1..254
FT                   /note="Alcohol dehydrogenase"
FT                   /id="PRO_0000054496"
FT   ACT_SITE        151
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10001"
FT   BINDING         10..33
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
FT   BINDING         138
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   254 AA;  27568 MW;  21F8641526F11C30 CRC64;
     MSLTNKNVVF VAGLGGIGLD TSRELVKRDL KNLVILDRID NAAAIAELKA INPKVTVTFY
     PYDVTVPVAE TTKLLKTIFA QIKTIDVLIN GAGILDDHQI ERTIAVNYSG LVNTTTAILD
     FWDKRKGGPG GIICNIGSVT GFNAIYQVPV YSGSKAAVVN FTSSLAKLAP ITGVTAYTVN
     PGITKTTLVH KFNSWLDVEP RVAEKLLEHP TQTSQQCAEN FVKAIELNKN GAIWKLDLGT
     LESITWTKHW DSGI