ADH_DROYA
ID ADH_DROYA Reviewed; 256 AA.
AC P26719; B4P0U4;
DT 01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Alcohol dehydrogenase;
DE EC=1.1.1.1;
GN Name=Adh; ORFNames=GE19037;
OS Drosophila yakuba (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7245;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Brazzaville;
RX PubMed=1904993; DOI=10.1038/351652a0;
RA McDonald J.H., Kreitman M.;
RT "Adaptive protein evolution at the Adh locus in Drosophila.";
RL Nature 351:652-654(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Gif-sur-Yvette stock 115;
RA Ashburner M.;
RL Submitted (JUL-1990) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tai18E2 / Tucson 14021-0261.01;
RX PubMed=17994087; DOI=10.1038/nature06341;
RG Drosophila 12 genomes consortium;
RT "Evolution of genes and genomes on the Drosophila phylogeny.";
RL Nature 450:203-218(2007).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a primary alcohol + NAD(+) = an aldehyde + H(+) + NADH;
CC Xref=Rhea:RHEA:10736, ChEBI:CHEBI:15378, ChEBI:CHEBI:15734,
CC ChEBI:CHEBI:17478, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.1;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU10001};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a secondary alcohol + NAD(+) = a ketone + H(+) + NADH;
CC Xref=Rhea:RHEA:10740, ChEBI:CHEBI:15378, ChEBI:CHEBI:17087,
CC ChEBI:CHEBI:35681, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.1;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU10001};
CC -!- SUBUNIT: Homodimer.
CC -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC family. {ECO:0000305}.
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DR EMBL; X57365; CAA40639.1; -; Genomic_DNA.
DR EMBL; X57366; CAA40640.1; -; Genomic_DNA.
DR EMBL; X57367; CAA40641.1; -; Genomic_DNA.
DR EMBL; X57368; CAA40642.1; -; Genomic_DNA.
DR EMBL; X57369; CAA40643.1; -; Genomic_DNA.
DR EMBL; X57370; CAA40644.1; -; Genomic_DNA.
DR EMBL; X57371; CAA40645.1; -; Genomic_DNA.
DR EMBL; X57372; CAA40646.1; -; Genomic_DNA.
DR EMBL; X57373; CAA40647.1; -; Genomic_DNA.
DR EMBL; X57374; CAA40648.1; -; Genomic_DNA.
DR EMBL; X57375; CAA40649.1; -; Genomic_DNA.
DR EMBL; X57376; CAA40650.1; -; Genomic_DNA.
DR EMBL; X54120; CAA38063.1; -; Genomic_DNA.
DR EMBL; CM000157; EDW89018.1; -; Genomic_DNA.
DR PIR; S18277; S18277.
DR PIR; S18278; S18278.
DR PIR; S18279; S18279.
DR PIR; S18280; S18280.
DR PIR; S18281; S18281.
DR PIR; S18282; S18282.
DR PIR; S18284; S18284.
DR PIR; S18287; S18287.
DR PIR; S20718; S20718.
DR RefSeq; XP_002089306.1; XM_002089270.2.
DR AlphaFoldDB; P26719; -.
DR SMR; P26719; -.
DR STRING; 7245.FBpp0264047; -.
DR EnsemblMetazoa; FBtr0265555; FBpp0264047; FBgn0013162.
DR GeneID; 6528253; -.
DR KEGG; dya:Dyak_GE19037; -.
DR eggNOG; KOG4169; Eukaryota.
DR HOGENOM; CLU_010194_2_16_1; -.
DR OMA; TTLQCAQ; -.
DR OrthoDB; 1053465at2759; -.
DR PhylomeDB; P26719; -.
DR ChiTaRS; Adh; fly.
DR Proteomes; UP000002282; Chromosome 2L.
DR GO; GO:0005829; C:cytosol; IEA:EnsemblMetazoa.
DR GO; GO:0032991; C:protein-containing complex; IEA:EnsemblMetazoa.
DR GO; GO:0008774; F:acetaldehyde dehydrogenase (acetylating) activity; IEA:EnsemblMetazoa.
DR GO; GO:0004022; F:alcohol dehydrogenase (NAD+) activity; IEA:UniProtKB-EC.
DR GO; GO:0042803; F:protein homodimerization activity; IEA:EnsemblMetazoa.
DR GO; GO:0006117; P:acetaldehyde metabolic process; IEA:EnsemblMetazoa.
DR GO; GO:0046164; P:alcohol catabolic process; IEA:EnsemblMetazoa.
DR GO; GO:0048149; P:behavioral response to ethanol; IEA:EnsemblMetazoa.
DR GO; GO:0006069; P:ethanol oxidation; IEA:EnsemblMetazoa.
DR GO; GO:0006734; P:NADH metabolic process; IEA:EnsemblMetazoa.
DR InterPro; IPR002425; ADH_Drosophila-type.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR020904; Sc_DH/Rdtase_CS.
DR InterPro; IPR002347; SDR_fam.
DR Pfam; PF00106; adh_short; 1.
DR PRINTS; PR01168; ALCDHDRGNASE.
DR PRINTS; PR00080; SDRFAMILY.
DR SUPFAM; SSF51735; SSF51735; 1.
DR PROSITE; PS00061; ADH_SHORT; 1.
PE 3: Inferred from homology;
KW NAD; Oxidoreductase.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250"
FT CHAIN 2..256
FT /note="Alcohol dehydrogenase"
FT /id="PRO_0000054501"
FT ACT_SITE 153
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10001"
FT BINDING 12..35
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 140
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT VARIANT 211
FT /note="H -> Q (in strain: Gif-sur-Yvette stock 115)"
FT VARIANT 213
FT /note="T -> N (in strain: Gif-sur-Yvette stock 115)"
FT VARIANT 233
FT /note="G -> R (in strain: Gif-sur-Yvette stock 115)"
SQ SEQUENCE 256 AA; 27716 MW; 2BA1ED90A9D275D5 CRC64;
MAFTLTNKNV VFVAGLGGIG LDTSKELVKR DLKNLVILDR IENPAAIAEL KAINPKVTVT
FYPYDVTVPI AETTKLLKTI FAQLKTIDVL INGAGILDDH QIERTIAVNY TGLVNTTTAI
LDFWDKRKGG PGGIICNIGS VTGFNAIYQV PVYSGTKAAV VNFTSSLAKL APITGVTAYT
VNPGITRTTL VHKFNSWLDV EPQVAEKLLA HPTQPSLACA QNFVKAIELN QNGAIWKLDL
GTLEAIQWSK HWDSGI
