ID   DYF4_CAEEL              Reviewed;         383 AA.
AC   Q17944;
DT   29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT   10-JUL-2007, sequence version 3.
DT   03-AUG-2022, entry version 106.
DE   RecName: Full=Protein dyf-4 {ECO:0000305};
DE   AltName: Full=Abnormal dye filling protein 4 {ECO:0000305};
DE   Flags: Precursor;
GN   Name=dyf-4 {ECO:0000312|WormBase:C13C12.2};
GN   ORFNames=C13C12.2 {ECO:0000312|WormBase:C13C12.2};
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN   [1] {ECO:0000312|Proteomes:UP000001940}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
RN   [2] {ECO:0000305}
RP   FUNCTION, INTERACTION WITH DAF-6, SUBCELLULAR LOCATION, TISSUE SPECIFICITY,
RP   DEVELOPMENTAL STAGE, AND MUTAGENESIS OF 1-MET--ALA-16 AND CYS-366.
RX   PubMed=34115759; DOI=10.1371/journal.pgen.1009618;
RA   Hong H., Chen H., Zhang Y., Wu Z., Zhang Y., Zhang Y., Hu Z., Zhang J.V.,
RA   Ling K., Hu J., Wei Q.;
RT   "DYF-4 regulates patched-related/DAF-6-mediated sensory compartment
RT   formation in C. elegans.";
RL   PLoS Genet. 17:e1009618-e1009618(2021).
CC   -!- FUNCTION: Required for the localization of daf-6 to the socket glial
CC       channel and the sheath lumen (PubMed:34115759). In association with
CC       daf-6, plays a role in dendrite extension and ciliogenesis to ensure
CC       the formation of glial channels in amphid and phasmid ciliated sensory
CC       neurons (PubMed:34115759). {ECO:0000269|PubMed:34115759}.
CC   -!- SUBUNIT: Interacts with daf-6. {ECO:0000269|PubMed:34115759}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:34115759}.
CC       Note=Secreted into the sensory compartment region in both amphids and
CC       phasmids. {ECO:0000269|PubMed:34115759}.
CC   -!- TISSUE SPECIFICITY: Expressed in sheath and socket glial cells in both
CC       the amphid and phasmid ciliated sensory neurons (at protein level).
CC       {ECO:0000269|PubMed:34115759}.
CC   -!- DEVELOPMENTAL STAGE: Expressed in early developing glial cells of the
CC       embryo and accumulates between the embryo and the eggshell.
CC       {ECO:0000269|PubMed:34115759}.
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DR   EMBL; BX284605; CAB01125.3; -; Genomic_DNA.
DR   PIR; T19230; T19230.
DR   RefSeq; NP_506139.3; NM_073738.6.
DR   AlphaFoldDB; Q17944; -.
DR   STRING; 6239.C13C12.2; -.
DR   EPD; Q17944; -.
DR   PaxDb; Q17944; -.
DR   PeptideAtlas; Q17944; -.
DR   EnsemblMetazoa; C13C12.2.1; C13C12.2.1; WBGene00007552.
DR   EnsemblMetazoa; C13C12.2.2; C13C12.2.2; WBGene00007552.
DR   EnsemblMetazoa; C13C12.2.3; C13C12.2.3; WBGene00007552.
DR   GeneID; 182568; -.
DR   KEGG; cel:CELE_C13C12.2; -.
DR   UCSC; C13C12.2; c. elegans.
DR   CTD; 182568; -.
DR   WormBase; C13C12.2; CE41198; WBGene00007552; dyf-4.
DR   eggNOG; ENOG502RY84; Eukaryota.
DR   HOGENOM; CLU_061040_0_0_1; -.
DR   InParanoid; Q17944; -.
DR   OMA; RYCTIFW; -.
DR   OrthoDB; 668181at2759; -.
DR   Proteomes; UP000001940; Chromosome V.
DR   Bgee; WBGene00007552; Expressed in embryo and 3 other tissues.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
PE   1: Evidence at protein level;
KW   Glycoprotein; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..16
FT                   /evidence="ECO:0000255"
FT   CHAIN           17..383
FT                   /note="Protein dyf-4"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_5004185744"
FT   CARBOHYD        64
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   MUTAGEN         1..16
FT                   /note="Missing: Impairs localization to the sensory
FT                   compartment and the protein accumulates in vesicular
FT                   structures in both amphid and phasmid ciliated sensory
FT                   neurons."
FT                   /evidence="ECO:0000269|PubMed:34115759"
FT   MUTAGEN         366
FT                   /note="C->Y: In jhu431; ciliogenesis defects resulting in
FT                   shorter cilia which lack distal segments with decreased
FT                   levels of intraflagellar transports proteins osm-6, osm-5,
FT                   che-11 and bbs-7 in cilia. Dendrite extension defects
FT                   resulting in short phasmid dendrites and truncated amphid
FT                   dendrites. Impairs localization to the sensory compartment
FT                   in amphids and phasmids and the protein accumulates in
FT                   glial cells."
FT                   /evidence="ECO:0000269|PubMed:34115759"
SQ   SEQUENCE   383 AA;  44126 MW;  0A5B73DA973A3D65 CRC64;
     MKTIWLLLAT CIHVFAHDEL YILIDEVSLY NCRGVKNEIT VKEEDVQIVN ERGNRVYYIR
     APGNYSLDFK KIKVKQNFGF LAGEIGVTLQ VPVLEGPAGI RFDLPYTMVP ETTLLSQKCD
     DFSGVIERNG RTYCRYCDLC HVSQAVENEL ASGRHQFLSQ SENDTPISKC YNIESNEYDF
     RRTIQLPSRS QLEGLIRSKA QGIDDEIKKR LNKGRGRFQV FLNLITSDKP AISLNRWMAG
     SKDCECCFNR NAPHCDSLSY LYCNMEDCKT GWALQCLHKS AKVAACYTVE FNYRMTTSYS
     DVLEFLRENN YPNQDSQYTQ PNQPLVPTTR RPVTPSFEAR QANQLQMTQA CVESMPSRMT
     HLKRYCTIFW NEKLCCEHCP DIC