ID   DYH5_RAT                Reviewed;        3470 AA.
AC   M0R8U1;
DT   25-OCT-2017, integrated into UniProtKB/Swiss-Prot.
DT   03-APR-2013, sequence version 1.
DT   03-AUG-2022, entry version 60.
DE   RecName: Full=Dynein axonemal heavy chain 5 {ECO:0000305};
DE   AltName: Full=Axonemal beta dynein heavy chain 5;
DE   AltName: Full=Ciliary dynein heavy chain 5;
DE   Flags: Fragment;
GN   Name=Dnah5 {ECO:0000312|RGD:1560828};
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND IDENTIFICATION.
RC   STRAIN=Brown Norway;
RX   PubMed=15057822; DOI=10.1038/nature02426;
RA   Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J.,
RA   Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G.,
RA   Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G.,
RA   Morgan M., Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G.,
RA   Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S.,
RA   Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T.,
RA   Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D.,
RA   Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L.,
RA   Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D.,
RA   Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M.,
RA   Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C.,
RA   Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J.,
RA   Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H.,
RA   Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X.,
RA   Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q.,
RA   Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P.,
RA   Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A.,
RA   Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C.,
RA   Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J.,
RA   Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J.,
RA   Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F.,
RA   Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A.,
RA   Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A.,
RA   Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J.,
RA   Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E.,
RA   Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M.,
RA   Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C.,
RA   Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L.,
RA   Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W.,
RA   Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y.,
RA   Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V.,
RA   Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M.,
RA   Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S.,
RA   Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B.,
RA   Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R.,
RA   Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J.,
RA   Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D.,
RA   Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S.,
RA   Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S.,
RA   Mockrin S., Collins F.S.;
RT   "Genome sequence of the Brown Norway rat yields insights into mammalian
RT   evolution.";
RL   Nature 428:493-521(2004).
RN   [2]
RP   SUBUNIT, AND INTERACTION WITH DNAL1.
RX   PubMed=21496787; DOI=10.1016/j.ajhg.2011.03.018;
RA   Mazor M., Alkrinawi S., Chalifa-Caspi V., Manor E., Sheffield V.C.,
RA   Aviram M., Parvari R.;
RT   "Primary ciliary dyskinesia caused by homozygous mutation in DNAL1,
RT   encoding dynein light chain 1.";
RL   Am. J. Hum. Genet. 88:599-607(2011).
CC   -!- FUNCTION: Force generating protein of respiratory cilia. Produces force
CC       towards the minus ends of microtubules. Dynein has ATPase activity; the
CC       force-producing power stroke is thought to occur on release of ADP.
CC       Required for structural and functional integrity of the cilia of
CC       ependymal cells lining the brain ventricles.
CC       {ECO:0000250|UniProtKB:Q8TE73}.
CC   -!- SUBUNIT: Interacts with DNAL1 (PubMed:21496787). Consists of at least
CC       two heavy chains and a number of intermediate and light chains.
CC       {ECO:0000250|UniProtKB:Q8TE73, ECO:0000269|PubMed:21496787}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, cilium axoneme
CC       {ECO:0000250|UniProtKB:Q8TE73}.
CC   -!- DOMAIN: Dynein heavy chains probably consist of an N-terminal stem
CC       (which binds cargo and interacts with other dynein components), and the
CC       head or motor domain. The motor contains six tandemly-linked AAA
CC       domains in the head, which form a ring. A stalk-like structure (formed
CC       by two of the coiled coil domains) protrudes between AAA 4 and AAA 5
CC       and terminates in a microtubule-binding site. A seventh domain may also
CC       contribute to this ring; it is not clear whether the N-terminus or the
CC       C-terminus forms this extra domain. There are four well-conserved and
CC       two non-conserved ATPase sites, one per AAA domain. Probably only one
CC       of these (within AAA 1) actually hydrolyzes ATP, the others may serve a
CC       regulatory function. {ECO:0000250|UniProtKB:Q8TE73}.
CC   -!- SIMILARITY: Belongs to the dynein heavy chain family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AABR07008948; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AABR07008948; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   SMR; M0R8U1; -.
DR   IntAct; M0R8U1; 1.
DR   MINT; M0R8U1; -.
DR   STRING; 10116.ENSRNOP00000065896; -.
DR   PaxDb; M0R8U1; -.
DR   RGD; 1560828; Dnah5.
DR   VEuPathDB; HostDB:ENSRNOG00000048363; -.
DR   eggNOG; KOG3595; Eukaryota.
DR   HOGENOM; CLU_000038_9_1_1; -.
DR   InParanoid; M0R8U1; -.
DR   OMA; DRTIAQX; -.
DR   Proteomes; UP000002494; Chromosome 2.
DR   Bgee; ENSRNOG00000048363; Expressed in heart and 3 other tissues.
DR   GO; GO:0097729; C:9+2 motile cilium; ISO:RGD.
DR   GO; GO:0005858; C:axonemal dynein complex; ISO:RGD.
DR   GO; GO:0005930; C:axoneme; ISS:UniProtKB.
DR   GO; GO:0005737; C:cytoplasm; ISO:RGD.
DR   GO; GO:0030286; C:dynein complex; IBA:GO_Central.
DR   GO; GO:0005576; C:extracellular region; IEA:GOC.
DR   GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR   GO; GO:0031514; C:motile cilium; ISS:UniProtKB.
DR   GO; GO:0036157; C:outer dynein arm; ISO:RGD.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0045505; F:dynein intermediate chain binding; IBA:GO_Central.
DR   GO; GO:0051959; F:dynein light intermediate chain binding; IBA:GO_Central.
DR   GO; GO:0003777; F:microtubule motor activity; ISO:RGD.
DR   GO; GO:0008569; F:minus-end-directed microtubule motor activity; IBA:GO_Central.
DR   GO; GO:0060271; P:cilium assembly; ISO:RGD.
DR   GO; GO:0003341; P:cilium movement; ISO:RGD.
DR   GO; GO:0007368; P:determination of left/right symmetry; ISO:RGD.
DR   GO; GO:0003351; P:epithelial cilium movement involved in extracellular fluid movement; ISO:RGD.
DR   GO; GO:0051649; P:establishment of localization in cell; ISO:RGD.
DR   GO; GO:0030317; P:flagellated sperm motility; ISO:RGD.
DR   GO; GO:0007507; P:heart development; ISO:RGD.
DR   GO; GO:0021670; P:lateral ventricle development; ISO:RGD.
DR   GO; GO:0007018; P:microtubule-based movement; IBA:GO_Central.
DR   GO; GO:0036158; P:outer dynein arm assembly; ISO:RGD.
DR   Gene3D; 1.10.8.710; -; 1.
DR   Gene3D; 1.20.140.100; -; 1.
DR   Gene3D; 3.20.180.20; -; 1.
DR   Gene3D; 3.40.50.300; -; 3.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR035699; AAA_6.
DR   InterPro; IPR026983; DHC_fam.
DR   InterPro; IPR041589; DNAH3_AAA_lid_1.
DR   InterPro; IPR042222; Dynein_2_N.
DR   InterPro; IPR043157; Dynein_AAA1S.
DR   InterPro; IPR041466; Dynein_AAA5_ext.
DR   InterPro; IPR024743; Dynein_HC_stalk.
DR   InterPro; IPR024317; Dynein_heavy_chain_D4_dom.
DR   InterPro; IPR013602; Dynein_heavy_linker.
DR   InterPro; IPR013594; Dynein_heavy_tail.
DR   InterPro; IPR042228; Dynein_linker_3.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR10676; PTHR10676; 1.
DR   Pfam; PF12774; AAA_6; 1.
DR   Pfam; PF12780; AAA_8; 1.
DR   Pfam; PF17857; AAA_lid_1; 1.
DR   Pfam; PF08385; DHC_N1; 1.
DR   Pfam; PF08393; DHC_N2; 1.
DR   Pfam; PF17852; Dynein_AAA_lid; 1.
DR   Pfam; PF12777; MT; 1.
DR   SMART; SM00382; AAA; 3.
DR   SUPFAM; SSF52540; SSF52540; 4.
PE   1: Evidence at protein level;
KW   ATP-binding; Cell projection; Cilium; Coiled coil; Cytoplasm; Cytoskeleton;
KW   Dynein; Microtubule; Motor protein; Nucleotide-binding; Reference proteome;
KW   Repeat.
FT   CHAIN           1..3470
FT                   /note="Dynein axonemal heavy chain 5"
FT                   /id="PRO_0000441779"
FT   REGION          1..1938
FT                   /note="Stem"
FT                   /evidence="ECO:0000250"
FT   REGION          899..918
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1939..2161
FT                   /note="AAA 1"
FT                   /evidence="ECO:0000250"
FT   REGION          2221..2440
FT                   /note="AAA 2"
FT                   /evidence="ECO:0000250"
FT   REGION          2547..2800
FT                   /note="AAA 3"
FT                   /evidence="ECO:0000250"
FT   REGION          2913..3167
FT                   /note="AAA 4"
FT                   /evidence="ECO:0000250"
FT   COILED          3207..3241
FT                   /evidence="ECO:0000255"
FT   COILED          3434..3468
FT                   /evidence="ECO:0000255"
FT   BINDING         1977..1984
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
FT   BINDING         2259..2266
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
FT   NON_TER         3470
SQ   SEQUENCE   3470 AA;  395933 MW;  BC2B495A5A3F7BC6 CRC64;
     MFRIGRRQLW KQSVTRVLTQ RLKEEKEAKR ARLDGRHDYL FAIVASCLDL NKPEVEDALL
     EGNQIERIDQ LFAVGGLRHL MFYYQDVEGA EAGQFGSSGG VNPASGKMKK PKVFVTEGKD
     VALMGACVFF TRADPSKAIT AENIHREVSF NTLDTADGGL LNSVRRLLSD IFIPALRASS
     HGWGELEGLQ DASSIQQEFL SSLEGFVGIL SGAQNSLKEK VNLQKCDIVE LKSLKEPMDY
     LALASNPETV EKVECCMRVW IKQMEQILAE NNQLRKEADD VGPRAELEHW KKRLSKFNYL
     LDQLKSPDVK AVLAMLAAAK SKLLKVWRDA DIRVTDAANE AKDNVKYLYT LEKCCDPLYS
     SDPVTMVDAI PTLINAIKMI YSISHYYNTS ERITSLFVKV TNQMISACKA HITNNGTATI
     WSQPQDIVMQ KIAAAIKLKQ GYQCCFQETK QKLKQNPSEK QFDFSEMYIF GKFETFHQRL
     AKIMDIFTTF KTYSVLQDSK IEGLEDMVTK YQDVVAGIKK KEYNFLDQRK MDFDQDYDEF
     CKQTNELHSE LQRFMDTTFE KIQSTRQALS TLKKFERLNI PNLGIEAKYQ IVFQNFGTDI
     DMISKLYTKQ KYDPPLARDQ PPIAGKILWA RQLFHRLEQP MQLFQQHPFV LRTVEAKPVI
     RSYNRIAKVL LEFEVLYHRA WLQQIEEIHV GLEASLLVKA PGTGQLFVNF DPQILILFRE
     TQCMSQMGLP VSPFAAALFE KRDMYKKNFS DMKMMLSEYQ RVKLKMPPAI EQLMLPHLAR
     VDEALQPGLA VLTWTSLNIG TYLENAFEKI KDLELLLDRI NDLIEFRIHA ILEEMSSVAL
     CQLPQDDPLT CEEFLQMTKD LCVNGAQKLH FKSSLVEEAV NELINMLLDV DVLPEEASEK
     VRHENASPNG DTSGGGEGCA EALASSFNAG TSSLPLTTIA RKKKEMEVLE EARELLSYFN
     HQNTDALLKV TRNTLEAIRR RIHFSHMINF RDSKGASKVK QNHLPIFRAS VTLAIPNISM
     TPALEDIQQT LNKAVECIIS VPKGVRQWSS ELLSKRKMRE RKMAAVQSNE DSDSDTEVEE
     SELQETLELA SVNLPIPVQT QNYYKNISDN KEIVKLVSVL STVISSTKKE VITSMDRFKC
     YNHIWQKEKE DTIMTFIAQN PLLSEFESRI LYFQSLEQEI NAEPEYICVG SIALYTADLK
     FSLTAETKAW MMVLGRHCNR KYRSEMENIF TVVEEFQKKL NRPIKDLDDI RIAMAALKEI
     REQQISTDFQ VGPIEESYAL LNKYGLLVAK EEMDKVDTLR YAWEKLLARA SDVQNELGAL
     QPSFRKELIS TVEVFLQDCQ QFYLDYDLNG PMVSGLKPQE ASDRLIIFQN QFDNIYRKYI
     TYTGGEELFG LPVTQYPQLL EIKKQLNLLQ KIYSLYNNVI ETVNSYQDTL WSEVNIEKIN
     SELLEFQNRC RKLPRALKDW QAFLDMKKTI DDFSECCPLL EYMASNAMVE RHWQRITTLT
     GHSLDVGNET FKLRNIMEVP LLKYKEEIED ICISAVKERD IEQKLKQVIN EWDNKTLTFS
     SFKTRGELLL RGDSTSEVIA SMEDSLMLLG SLLSNRYNMP FKAQIQNWVQ CLSNSTDIIE
     NWMTVQNLWI YLEAVFVGGD IAKQLPKEAK RFSNIDKSWV KIMTRAHEIP NVVQCCVGDE
     TMGQLLPHLL DQLEICQKSL TGYLEKKRLC FPRFFFVSDP ALLEILGQAS DSHTIQAHLL
     NVFDNIKTVK FHDKIYDRIL SISSREGETI ELDKPVMAEG NVEVWLNSLL EESQSSLHLV
     IRQAAANIQE SGFQLIEFLS SFPAQVGLLG IQMLWTRDSE EALQNAKFDK KIMQKTNQSF
     LELLNMLIEM TTKDLSSMER VKYETLITIH VHQRDIFDDL CHMHVKSPTD FEWLKQCRFY
     FKEDSDKTMI HITDVAFTYQ NEFLGCTDRL VITPLTDRCY ITLAQALGMS MGGAPAGPAG
     TGKTETTKDM GRCLGKYVVV FNCSDQMDFR GLGRIFKGLA QSGSWGCFDE FNRIDLPVLS
     VAAQQISIIL TCKKEHKKSF IFTDGDNVTM NPEFGLFLTM NPGYAGRQEL PENLKINFRS
     VAMMVPDRQI IIRVKLASCG FIDNVVLARK FFTLYQLCEE QLSKQVHYDF GLRNILSVLR
     TLGAAKRASH TDTESTIVMR VLRDMNLSKL IDEDEPLFLS LIEDLFPNIL LDKAGYPELE
     TAISKQVEEA GLINHPPWKL KVIQLFETQR VRHGMMTLGP SGSGKTSCIH TLMKAMTDCG
     KPHREMRMNP KAITAPQMFG RLDVATNDWT DGIFSTLWRK TLKAKKGEHI WIVLDGPVDA
     IWIENLNSVL DDNKTLTLAN GDRIPMAPNC KIVFEPHNID NASPATVSRN GMVFMSSSVL
     DWSPILEGFL KRRSPQEAEI LRQLYAETFP DLYRFSIQNL EFKMEILEAF VITQSTHMLQ
     GLIPTKEQAG DVDPEHLGRL FVFAMMWSVG AVLELEGRRR MELWLRSREG PTLHLPQLTD
     PGDTMFDYYV APDGTWRHWS MCIPEYVYPP DTTPEYGSIL VPNVDNVRTD FLIKTIAKQG
     KAVLLIGEQG TAKTVIIKGF MSKFDPESHT VKNLNFSSAT TPLMFQRTIE SYVDKRMGTT
     YGPPAGKKMA VFIDDLNMPV INEWGDQVTN EIVRQLMEQN GFYNLEKPGE FTSIVDIQFL
     AAMIHPGGGR NDIPQRLKRQ FSIFNCTLPS DASMDKIFGV IGEGYYCAQR GFSKEVQDAV
     IKLVPLTRRL WQMTKLKMLP TPAKFHYVFN LRDLSRIWQG MLNITSEVIK DTDELLRLWK
     HECKRVIADR FSMSSDVTWF DKAVVSLVEE EFGEEKTPVV DCGVDAYFVD FLRDAPEATG
     ETPEETDAEM PKLYEPIASL NHLQERLSVF LQLYNESIRG TGMDMVFFRD AMVHLVKISR
     VIRTPRGNAL LVGVGGSGKQ SLTRLASFIA GYTSFQITLT RSYNTSNLME DLKVLYRTAG
     QQGKGITFIF TDNEIKEESF LEYMNNVLSS GEVSNLFARD EIDEINSDLT SIMKKEHPKR
     PPTNDNLYEY FMSRVRGNLH IVLCFSPVGE KFRNRALKFP ALISGCTIDW FSRWPKDALV
     AVSEHFLSSY NIDCTAEIKK ELVQCMGSFQ DGVAEKCADY FQRFRRSTHV TPKSYLSFIQ
     GYKFIYEEKH VEVQSLANRM NTGLEKLKEA SESVAALSQE LAVKEKELQV ANEKADMVLK
     EVTMKAQAAE KVKAEVQKVK DKAQAIVDSI SKDKAIAEEK LEAAKPALEE AEAALQTIKP
     SDIATVRTLG RPPHLIMRIM DCVLLLFHRR VNAVKIDLDK SCTVPSWQES LKLMTAGNFL
     QNLQQFPKDT INEEVIEFLS PYFEMSDYNI ETAKRVCGNV AGLCSWTKAM ASFFSINKEV
     LPLKANLIVQ ENRHALAMQD LQKAQAELDD KQAELDVVQA EYEQAMTEKQ