ID   DYHB_CHLRE              Reviewed;        4568 AA.
AC   Q39565;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   03-AUG-2022, entry version 87.
DE   RecName: Full=Dynein beta chain, flagellar outer arm;
GN   Name=ODA4; Synonyms=ODA-4, SUP1;
OS   Chlamydomonas reinhardtii (Chlamydomonas smithii).
OC   Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC   CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX   NCBI_TaxID=3055;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=21gr / CC-1690;
RX   PubMed=8006077; DOI=10.1242/jcs.107.3.635;
RA   Mitchell D.R., Brown K.S.;
RT   "Sequence analysis of the Chlamydomonas alpha and beta dynein heavy chain
RT   genes.";
RL   J. Cell Sci. 107:635-644(1994).
CC   -!- FUNCTION: Force generating protein of eukaryotic cilia and flagella.
CC       Produces force towards the minus ends of microtubules. Dynein has
CC       ATPase activity; the force-producing power stroke is thought to occur
CC       on release of ADP.
CC   -!- SUBUNIT: Consists of at least 3 heavy chains (alpha, beta and gamma), 2
CC       intermediate chains and 8 light chains.
CC   -!- SUBCELLULAR LOCATION: Cell projection, cilium, flagellum. Cytoplasm,
CC       cytoskeleton, flagellum axoneme.
CC   -!- DOMAIN: Dynein heavy chains probably consist of an N-terminal stem
CC       (which binds cargo and interacts with other dynein components), and the
CC       head or motor domain. The motor contains six tandemly-linked AAA
CC       domains in the head, which form a ring. A stalk-like structure (formed
CC       by two of the coiled coil domains) protrudes between AAA 4 and AAA 5
CC       and terminates in a microtubule-binding site. A seventh domain may also
CC       contribute to this ring; it is not clear whether the N-terminus or the
CC       C-terminus forms this extra domain. There are four well-conserved and
CC       two non-conserved ATPase sites, one per AAA domain. Probably only one
CC       of these (within AAA 1) actually hydrolyzes ATP, the others may serve a
CC       regulatory function.
CC   -!- SIMILARITY: Belongs to the dynein heavy chain family. {ECO:0000305}.
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DR   EMBL; U02963; AAA19956.1; -; Genomic_DNA.
DR   PIR; T08030; T08030.
DR   SMR; Q39565; -.
DR   STRING; 3055.EDP01834; -.
DR   PRIDE; Q39565; -.
DR   eggNOG; KOG3595; Eukaryota.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0030286; C:dynein complex; IEA:UniProtKB-KW.
DR   GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR   GO; GO:0031514; C:motile cilium; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0045505; F:dynein intermediate chain binding; IEA:InterPro.
DR   GO; GO:0051959; F:dynein light intermediate chain binding; IEA:InterPro.
DR   GO; GO:0008569; F:minus-end-directed microtubule motor activity; IEA:InterPro.
DR   GO; GO:0030030; P:cell projection organization; IEA:UniProtKB-KW.
DR   GO; GO:0007018; P:microtubule-based movement; IEA:InterPro.
DR   Gene3D; 1.10.8.710; -; 1.
DR   Gene3D; 1.10.8.720; -; 1.
DR   Gene3D; 1.20.140.100; -; 1.
DR   Gene3D; 3.10.490.20; -; 1.
DR   Gene3D; 3.20.180.20; -; 1.
DR   Gene3D; 3.40.50.300; -; 5.
DR   InterPro; IPR035699; AAA_6.
DR   InterPro; IPR035706; AAA_9.
DR   InterPro; IPR041658; AAA_lid_11.
DR   InterPro; IPR042219; AAA_lid_11_sf.
DR   InterPro; IPR026983; DHC_fam.
DR   InterPro; IPR041589; DNAH3_AAA_lid_1.
DR   InterPro; IPR042222; Dynein_2_N.
DR   InterPro; IPR043157; Dynein_AAA1S.
DR   InterPro; IPR041466; Dynein_AAA5_ext.
DR   InterPro; IPR041228; Dynein_C.
DR   InterPro; IPR043160; Dynein_C_barrel.
DR   InterPro; IPR024743; Dynein_HC_stalk.
DR   InterPro; IPR024317; Dynein_heavy_chain_D4_dom.
DR   InterPro; IPR004273; Dynein_heavy_D6_P-loop.
DR   InterPro; IPR013602; Dynein_heavy_linker.
DR   InterPro; IPR013594; Dynein_heavy_tail.
DR   InterPro; IPR042228; Dynein_linker_3.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR10676; PTHR10676; 1.
DR   Pfam; PF12774; AAA_6; 1.
DR   Pfam; PF12780; AAA_8; 1.
DR   Pfam; PF12781; AAA_9; 1.
DR   Pfam; PF17857; AAA_lid_1; 1.
DR   Pfam; PF18198; AAA_lid_11; 1.
DR   Pfam; PF08385; DHC_N1; 1.
DR   Pfam; PF08393; DHC_N2; 1.
DR   Pfam; PF17852; Dynein_AAA_lid; 1.
DR   Pfam; PF18199; Dynein_C; 1.
DR   Pfam; PF03028; Dynein_heavy; 1.
DR   Pfam; PF12777; MT; 1.
DR   SUPFAM; SSF52540; SSF52540; 4.
PE   3: Inferred from homology;
KW   ATP-binding; Cell projection; Cilium; Cilium biogenesis/degradation;
KW   Coiled coil; Cytoplasm; Cytoskeleton; Dynein; Flagellum; Microtubule;
KW   Motor protein; Nucleotide-binding; Repeat.
FT   CHAIN           1..4568
FT                   /note="Dynein beta chain, flagellar outer arm"
FT                   /id="PRO_0000114649"
FT   REGION          1..1880
FT                   /note="Stem"
FT                   /evidence="ECO:0000250"
FT   REGION          1144..1166
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1881..2102
FT                   /note="AAA 1"
FT                   /evidence="ECO:0000250"
FT   REGION          2164..2385
FT                   /note="AAA 2"
FT                   /evidence="ECO:0000250"
FT   REGION          2493..2738
FT                   /note="AAA 3"
FT                   /evidence="ECO:0000250"
FT   REGION          2841..3090
FT                   /note="AAA 4"
FT                   /evidence="ECO:0000250"
FT   REGION          3106..3425
FT                   /note="Stalk"
FT                   /evidence="ECO:0000250"
FT   REGION          3481..3711
FT                   /note="AAA 5"
FT                   /evidence="ECO:0000250"
FT   REGION          3937..4172
FT                   /note="AAA 6"
FT                   /evidence="ECO:0000250"
FT   COILED          277..293
FT                   /evidence="ECO:0000255"
FT   COILED          1158..1175
FT                   /evidence="ECO:0000255"
FT   COILED          1372..1400
FT                   /evidence="ECO:0000255"
FT   COILED          1614..1650
FT                   /evidence="ECO:0000255"
FT   COILED          1778..1825
FT                   /evidence="ECO:0000255"
FT   COILED          2831..2848
FT                   /evidence="ECO:0000255"
FT   COILED          3106..3162
FT                   /evidence="ECO:0000255"
FT   COILED          3339..3425
FT                   /evidence="ECO:0000255"
FT   COILED          3648..3728
FT                   /evidence="ECO:0000255"
FT   BINDING         1919..1926
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
FT   BINDING         2202..2209
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
FT   BINDING         2530..2537
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
FT   BINDING         2879..2886
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   4568 AA;  519972 MW;  9A9A5393C7C36AE7 CRC64;
     MAEDEGMTAA APSSSVKDIR FDYIRDRVCS CLKVPDSAYD KLVSGDGRTS LVQFMEEAHT
     KRLLIMLDGK DLSATVKPPP KFKKKTVYFL KLQETKLDND NIKKLVIHGE ISENPLETLA
     AISQDVFMPV LTAPANQQGW PDVVAKEVTE NLHKFVSNVF VTIGQMKGQT LLPLPPQNTV
     PTLQPEQSMH SLKDQDKIHI LESAIVTWTK QIKNVLKADP DAPLKEPGAY PGPLTELNFW
     SERAANLNSI HEQLTSEKTQ KVVKVLELAK STYYPAFQRL FREVEAAQQE ANDNVKFLKP
     LRKYLDKLNM MDDFPMLVDL FKPIMHTLML IWKHSKSYNS STRFVTLMQE ICNDLIMQAC
     KYVPGSDLIQ MEPSEAVDKL RMTLRWLGTF KNYYFEYRAL SMQDTPENPW KFQNNSLFAR
     LDSFLERCHD MMDLMSTCMQ FNRLERVEIG GTKGKVLTNG VKAIHQDFTS AVEKFQQVTY
     DVMDVDAKQF DEDFFGFRVV IKELERRLAA IIIQAFDDCT TIGTTFKLLD SFEGLLDREV
     IAHDLEKKHT DLLHSYARDL KDVADLFHQY KDRPIVAKNS APYSGAAYWV RGLMERIKDP
     MDRLLTMNKM VLESELFREI QRTYDHLWEE MTEYRTRAVD AWCAQVAATS DEKLNLPLLS
     LIEETADGIR VLGVNFDPAL VRLLRETKYF LLLETSTQDK NADRNAEKAA EGGEVEVVKK
     APKLSVPDSA KDLFASADTF RQQISALDLI CSIYNKVQRT ILAVEKPLVQ QKLDAVEQAL
     NRGLAELNWK CAEIDTYIKE CMELVKDVDL VLNTIKDNVK ATQGILAMWE KNLMFERKDG
     KTYTFDELND AFNQLIQQRH SEIRDAGKEI TKLLSSSNRV LKVSKGAASW RAYVDYFSNI
     VIDGFSAAII STVRYLLSQI DPDILAKTES SPLLEIQVEL VAPDIVWKPD LGEGGAKPGL
     RDMIKKWLQS FLEIGQLMKR LDVGEGNYAK ELEEDFEVYD ALNQVMMVTL ANESRCEDFK
     NQFAKFDYLW KQDLQATLQQ FITDNGVTLP DGTRDDPPLA KFEEQIVKYK NVASEIASFK
     DTMTMGYVKV NAKPLRQALS TWASKWVYLF THYLQEKVVN SITELYTFMD TSNSTLDLKV
     MGEGVEEEPE YHPDQDPEEA AAKKAAEEEE KRKALYAIMA CMRDIRRRTE RGTDTMFEPL
     KETVTALHTF GIQLSDTVLH QLDNAEFNWR TLKKKMLNRR EQLAPLQQAE AVEIRRKSDA
     FNERVEDFRT FFQRKAPFAV SGGELKLEQV KPAYKLLDEF RSGSLEGYPS VLGIIAESKQ
     LQEAQDLFEL YQPGYLQLQR CSEELGHLKS LWDTVGTVMF TFRDWYKTPW DKIDVDFLVE
     ETKKLSKDIK MLNKAVRNYD VYRMLEEAIK AVLTSLPLVQ DLHHPAMRER HWKLLMQTTG
     KHFVMDDKFC LGDLLALELH NYVDACSEIV DRAQKELNIE KQLKKIEDTW AGLSLAFSTY
     QDSDVMALLV DDAVNEALEA DNLQLQNLSG QKYVQSNPMF LETVSKWQNN MGRVSAVLET
     WQNVQKKWQN LESIFIGSAD IRVQLPEDSK RFDAVNADFQ ELMRTAPDIT NVVEACTLDG
     RQERLENMQS MLEQCEKALQ EYLETKRVAF PRFYFVSPAD LLDILSKGSN PQLILRHLQK
     CFDNIDNLSF RKDERGDPTK IATHMHSKEG EVVEFVEDCS CDGPVEVWLQ NVVDSMKLAL
     QVEFRKAIPT YDELPRTKWI YVYSAQNTVV VSRTFFTQEI NEAFDDLEEG NEEALKVELD
     RQVQQLADLI DEINKEQTSL DRKKLITLCT IDVHSRDLVQ KLIDERVEDQ MCFQWQSQLR
     YIQSEKTKTC QVNICDAEIA YSYEYIGNCG CLCITPLTDR CFITLTQAQR LVLGGAPAGP
     AGTGKTETTK DLARALGIQC YVFNCSDQMD YKAMGHTYKG LAQTGAWGCF DEFNRIPVAV
     LSVCSTQYKT VLDAIRAKKE RFTFEDADIS LKSTVMAFIT MNPGYPGRAE LPESLKALFR
     PVSMVVPDLA LICEIMLMAE GFQMSKILSR KFVILYKLCE DLLSKSRHYD WKLRAIKTTL
     YVAGGMKRAA PELSEDKVLL RALRDFNLGK LTADDTSIFM GLLNDLFPKT LELVPRALDK
     AFDEAAHKAA TELGYQPDDQ FLLKISHVRE LFVVRWSVFL LGAAGCGKTA VWRTLLRAQN
     SSGEKTIYQA VNPKAVTRNE LYGYLHPATR EWKEGLMSVT FRNMANNKTN KHQWIVLDGD
     IDAEWIESMN TVMDDNKMLT LASNERIPLT PSMRLLLEIN HMVHCSPATV SRGGVIFINA
     DDVGWQPVVA SWIDKLEAAE YRPLLTALFT KYVDPCLEHC RRNFKTVVPL PAVNQAMTIC
     KILEGILPKE TVRGAPPPDK KLLHYHFVFA CVWAFGGCML VDKVTDYRTQ FSKWWVSEWK
     DVQFPEKGLV YDYYVDEQNC IMVPWEDRVT KFQYIPGDFT SLFVPTVETT RLTYFLDSLV
     SNKHYAMFVG NTGTGKSAIM VNKLRNMDTE TMSFYTINMN SLSEAPALQV ILEQPLEKKS
     GVRYGPPGSR RMVYFVDDMN MPLVDKYDTQ SSIELLRQMV DYHGWYDKVK IQLKEIINCQ
     MAACMNPTAG SFNITPRMQR HFVTFAVQMP NAEITRAMYY QIIDGHFSSF DVDVAKMSNK
     LVDATCELHR NVMHNFLPSA VKFHYQFNLR DLSNITQGLT RAIKEYYREP VKVARLWVHE
     CERVFRDRMI NEADMAKFDE FRVAVTKKFF DDCGGMVAIE ERPLIYASHA SMTYTPEDVP
     VYNALSSYDV LRKTLEDKLR EYNESNAVMD LVLFQQAMEH VTRIARIIDL PRGNAMLVGV
     GGSGKQSLAR LASYICGYEV YQISVSSTYG INDFKENLLG LYRKAGTKGT PITFLMTDNQ
     IVKEGFLVYI NDLLSTGYIA DLFTPEDKEA FTNAVRNEVK AAGILDSAEN CWDFFIDKVR
     KFLHIVLCFS PVGDKFRIRA RQFPALVNCT MFDWFHGWPG EALVSVAQRF LVDVPNMEEV
     VRENIAYHMA YAHQCVSEAS ERFKEAFRRY NYTTPKSYLE LISLYKMLLQ LKRDDLRRSK
     ERLENGIDKI AQAAAQVTDL QRVLKEEQIV VDEKKAQTDE LIVSIGKEKA IVDQAVEAGR
     EDEEAATALQ TEVSAFQAEC ERDLLEAEPI IAQAEAALNS LNKKELSELK SFGSPAAEIV
     QVAAACLVLT CGGKIPKDRD WNAGKKMMAD VNSFLSSLMN FDKDNVPVVC VEVVEKDYIS
     NPGFTPDNIK GKSAACAGLC SWVINICKYF RIYQVVAPKR AALAEANKKL DTANKKLKVI
     RDEVKRLQDR VALLEQSLMK ATEDKNAAIA QADRTARKAQ MAERLINGLS GENTRWGAEI
     KRLESLEGRL VGDVLIASAF VSYAGPFNMQ FRKSLVDEKW LPDIIERQIP MTQGIRPLDL
     LTDDATKAKW ANEGLPTDPL SVENGAIMSN ASRWALMIDP QLQGIKWIIN KETNNGLVII
     QQSQPKYIDQ VINCIENGWP LLIENLPVDI DAVLDPVIGK MTIKKGRNII MKIGDAEVQY
     DSRFRLYLQT KLSNPHFKPE VAAQTTLVNF CVTEKGLEDQ LLALVVDHER PDLQEQAAGL
     VRSLNEYNIT LVELENNLLF NLANATGNIL ENIELIEGLE ETKRTAVEIE EKVKLAKQTE
     IQIAKAREVY RPVATRGSLT YFLIDNLNAL DRVYHYSMAN FVFVLKKGMD MTPGGKDESK
     VPLAERLNQE VDLDKRVELL VETTCFVLIG YVAQGLFERH KLIVATQLCM QILRSRGELH
     YAKFEYLLRG PKVMGADNPL HDWVSDSVWG SVQALKELDD YQGLPEDLIG SSKRWREWME
     LERPEDEPLP GDWKRMQEFD KLLLFRALRP DRLTSAMGRF VTNMLGAKYV TSQPYDLERS
     YQDASPGTPI FVFLSPGVDV AGSVEALGKK LGFTLDNGKY ASVSLGQGQE PIAMDRLSAA
     HKNGGWVLLQ NIHLTIDWTT NQLDKKVDKL VEGAHPDFRL FLSAEPPPSL ERGLPISLLQ
     NSIKLTNEPA RGLKANLRRA WNNFNEEILE SCAKQAEFRA IVFALCYFHA ALLERKKFGV
     GNLPGARSGI GWNMNYPFNT GDLLCCGQTA NNYLENNVKV PWEDLRYNFG EIMYGGHIVE
     DYDRRLAMCY LRKYVNEGLL DNMEFFPGFA MPPNTANHRQ VLEFIDEVMP PETPLAFGLH
     PNAEIGFKLR EAESFCNSLV QLQPRESSGE GGMSAEERAK LVLDEVVDKL PDIFDMEDVR
     SKINPDDPNM PFVMVAIQES ERMNMLLAEM KRSLLELDLG LKGDLTMTEP MERLLKALAT
     DAVPGSWRNL AYPSLRPLGS WLGNLLARHA QLVDWTAELS TPKAVWLSGL FNPQSFLTAV
     MQATARRNDW PLDKTVIITE VTKKQPDQIE ANSRDGAFIH GLTLEGARWD DKIGALDDSK
     PKELFCPMPV ILVRAVTQDK AEMKDVYKCP VYTTEARFRE EVFEAQLKSK HTEIKWVLAG
     VCLFLDVV