DYHC2_RAT
ID DYHC2_RAT Reviewed; 4306 AA.
AC Q9JJ79; P70576; Q63167;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=Cytoplasmic dynein 2 heavy chain 1;
DE AltName: Full=Cytoplasmic dynein 2 heavy chain;
DE AltName: Full=Dynein cytoplasmic heavy chain 2;
DE AltName: Full=Dynein heavy chain isotype 1B;
DE AltName: Full=Dynein-like protein 4;
GN Name=Dync2h1; Synonyms=Dhc1b, Dlp4, Dnch2, Dnchc2;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH DYNC2LI1, AND SUBCELLULAR
RP LOCATION.
RC STRAIN=Sprague-Dawley; TISSUE=Testis;
RX PubMed=12432068; DOI=10.1242/jcs.00168;
RA Mikami A., Tynan S.H., Hama T., Luby-Phelps K., Saito T., Crandall J.E.,
RA Besharse J.C., Vallee R.B.;
RT "Molecular structure of cytoplasmic dynein 2 and its distribution in
RT neuronal and ciliated cells.";
RL J. Cell Sci. 115:4801-4808(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 1665-1744.
RC TISSUE=Kidney;
RX PubMed=8812413; DOI=10.1006/geno.1996.0422;
RA Vaughan K.T., Mikami A., Paschal B.M., Holzbaur E.L.F., Hughes S.M.,
RA Echeverri C.J., Moore K.J., Gilbert D.J., Copeland N.G., Jenkins N.A.,
RA Vallee R.B.;
RT "Multiple mouse chromosomal loci for dynein-based motility.";
RL Genomics 36:29-38(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 1670-1860, TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RC STRAIN=Wistar; TISSUE=Brain;
RX PubMed=7657712; DOI=10.1242/jcs.108.5.1883;
RA Tanaka Y., Zhang Z., Hirokawa N.;
RT "Identification and molecular evolution of new dynein-like protein
RT sequences in rat brain.";
RL J. Cell Sci. 108:1883-1893(1995).
RN [4]
RP SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=8832411; DOI=10.1242/jcs.109.7.1891;
RA Criswell P.S., Ostrowski L.E., Asai D.J.;
RT "A novel cytoplasmic dynein heavy chain: expression of DHC1b in mammalian
RT ciliated epithelial cells.";
RL J. Cell Sci. 109:1891-1898(1996).
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=9450951; DOI=10.1091/mbc.9.2.237;
RA Criswell P.S., Asai D.J.;
RT "Evidence for four cytoplasmic dynein heavy chain isoforms in rat testis.";
RL Mol. Biol. Cell 9:237-247(1998).
RN [6]
RP INTERACTION WITH DYNC2LI1.
RX PubMed=11907264; DOI=10.1091/mbc.01-08-0402;
RA Grissom P.M., Vaisberg E.A., McIntosh J.R.;
RT "Identification of a novel light intermediate chain (D2LIC) for mammalian
RT cytoplasmic dynein 2.";
RL Mol. Biol. Cell 13:817-829(2002).
CC -!- FUNCTION: May function as a motor for intraflagellar retrograde
CC transport. Functions in cilia biogenesis. May play a role in transport
CC between endoplasmic reticulum and Golgi or organization of the Golgi in
CC cells (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: The cytoplasmic dynein complex 2 is probably composed by a
CC heavy chain DYNC2H1 homodimer and a number of DYNC2LI1 light
CC intermediate chains.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, cilium axoneme
CC {ECO:0000269|PubMed:12432068}. Cell membrane
CC {ECO:0000250|UniProtKB:Q45VK7}; Peripheral membrane protein
CC {ECO:0000250|UniProtKB:Q45VK7}. Cytoplasm {ECO:0000269|PubMed:12432068,
CC ECO:0000269|PubMed:8832411}. Note=Localizes to the apical cytoplasm
CC (PubMed:8832411). May localize to Golgi apparatus, cytoplasmic vesicle
CC and endoplasmic reticulum (By similarity). {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Widely expressed both in ciliated and unciliated
CC tissues. Detected in brain and testis (at protein level).
CC {ECO:0000269|PubMed:7657712, ECO:0000269|PubMed:8832411,
CC ECO:0000269|PubMed:9450951}.
CC -!- DEVELOPMENTAL STAGE: Expressed in adult and juvenile brain.
CC {ECO:0000269|PubMed:7657712}.
CC -!- INDUCTION: Up-regulated during ciliogenesis.
CC {ECO:0000269|PubMed:8832411}.
CC -!- SIMILARITY: Belongs to the dynein heavy chain family. {ECO:0000305}.
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DR EMBL; AB041881; BAA97048.1; -; mRNA.
DR EMBL; U61748; AAC52802.1; -; mRNA.
DR EMBL; D26495; BAA05503.1; -; mRNA.
DR PIR; I70174; I70174.
DR RefSeq; NP_075413.1; NM_023024.1.
DR SMR; Q9JJ79; -.
DR BioGRID; 249321; 1.
DR CORUM; Q9JJ79; -.
DR STRING; 10116.ENSRNOP00000043252; -.
DR CarbonylDB; Q9JJ79; -.
DR iPTMnet; Q9JJ79; -.
DR PhosphoSitePlus; Q9JJ79; -.
DR jPOST; Q9JJ79; -.
DR PaxDb; Q9JJ79; -.
DR PRIDE; Q9JJ79; -.
DR GeneID; 65209; -.
DR KEGG; rno:65209; -.
DR UCSC; RGD:71042; rat.
DR CTD; 79659; -.
DR RGD; 71042; Dync2h1.
DR eggNOG; KOG3595; Eukaryota.
DR InParanoid; Q9JJ79; -.
DR OrthoDB; 26380at2759; -.
DR PhylomeDB; Q9JJ79; -.
DR Reactome; R-RNO-5610787; Hedgehog 'off' state.
DR Reactome; R-RNO-5620924; Intraflagellar transport.
DR PRO; PR:Q9JJ79; -.
DR Proteomes; UP000002494; Unplaced.
DR GO; GO:0045177; C:apical part of cell; ISO:RGD.
DR GO; GO:0005930; C:axoneme; ISO:RGD.
DR GO; GO:0005868; C:cytoplasmic dynein complex; ISO:RGD.
DR GO; GO:0030286; C:dynein complex; IBA:GO_Central.
DR GO; GO:0005794; C:Golgi apparatus; ISO:RGD.
DR GO; GO:0005874; C:microtubule; ISO:RGD.
DR GO; GO:0031514; C:motile cilium; ISO:RGD.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0045505; F:dynein intermediate chain binding; IBA:GO_Central.
DR GO; GO:0051959; F:dynein light intermediate chain binding; IPI:WormBase.
DR GO; GO:0008569; F:minus-end-directed microtubule motor activity; IBA:GO_Central.
DR GO; GO:0060271; P:cilium assembly; ISO:RGD.
DR GO; GO:0060976; P:coronary vasculature development; ISO:RGD.
DR GO; GO:0007368; P:determination of left/right symmetry; ISO:RGD.
DR GO; GO:0009953; P:dorsal/ventral pattern formation; ISO:RGD.
DR GO; GO:0030326; P:embryonic limb morphogenesis; ISO:RGD.
DR GO; GO:0030900; P:forebrain development; ISO:RGD.
DR GO; GO:0007030; P:Golgi organization; ISO:RGD.
DR GO; GO:0007507; P:heart development; ISO:RGD.
DR GO; GO:0035721; P:intraciliary retrograde transport; ISO:RGD.
DR GO; GO:0001822; P:kidney development; ISO:RGD.
DR GO; GO:0007018; P:microtubule-based movement; IBA:GO_Central.
DR GO; GO:0030182; P:neuron differentiation; ISO:RGD.
DR GO; GO:1905515; P:non-motile cilium assembly; ISO:RGD.
DR GO; GO:0045880; P:positive regulation of smoothened signaling pathway; ISO:RGD.
DR GO; GO:0061512; P:protein localization to cilium; ISO:RGD.
DR GO; GO:0016485; P:protein processing; ISO:RGD.
DR GO; GO:0021522; P:spinal cord motor neuron differentiation; ISO:RGD.
DR Gene3D; 1.10.8.710; -; 1.
DR Gene3D; 1.10.8.720; -; 1.
DR Gene3D; 1.20.140.100; -; 1.
DR Gene3D; 3.10.490.20; -; 1.
DR Gene3D; 3.20.180.20; -; 1.
DR Gene3D; 3.40.50.300; -; 5.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR035699; AAA_6.
DR InterPro; IPR035706; AAA_9.
DR InterPro; IPR041658; AAA_lid_11.
DR InterPro; IPR042219; AAA_lid_11_sf.
DR InterPro; IPR026983; DHC_fam.
DR InterPro; IPR026815; DYNC2H1.
DR InterPro; IPR042222; Dynein_2_N.
DR InterPro; IPR043157; Dynein_AAA1S.
DR InterPro; IPR041228; Dynein_C.
DR InterPro; IPR043160; Dynein_C_barrel.
DR InterPro; IPR024743; Dynein_HC_stalk.
DR InterPro; IPR024317; Dynein_heavy_chain_D4_dom.
DR InterPro; IPR004273; Dynein_heavy_D6_P-loop.
DR InterPro; IPR013602; Dynein_heavy_linker.
DR InterPro; IPR013594; Dynein_heavy_tail.
DR InterPro; IPR042228; Dynein_linker_3.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR10676; PTHR10676; 1.
DR PANTHER; PTHR10676:SF352; PTHR10676:SF352; 1.
DR Pfam; PF12774; AAA_6; 1.
DR Pfam; PF12780; AAA_8; 1.
DR Pfam; PF12781; AAA_9; 1.
DR Pfam; PF18198; AAA_lid_11; 1.
DR Pfam; PF08385; DHC_N1; 1.
DR Pfam; PF08393; DHC_N2; 1.
DR Pfam; PF18199; Dynein_C; 1.
DR Pfam; PF03028; Dynein_heavy; 1.
DR Pfam; PF12777; MT; 1.
DR SMART; SM00382; AAA; 3.
DR SUPFAM; SSF52540; SSF52540; 4.
PE 1: Evidence at protein level;
KW ATP-binding; Cell membrane; Cell projection; Cilium;
KW Cilium biogenesis/degradation; Coiled coil; Cytoplasm; Cytoskeleton;
KW Developmental protein; Dynein; Membrane; Microtubule; Motor protein;
KW Nucleotide-binding; Reference proteome.
FT CHAIN 1..4306
FT /note="Cytoplasmic dynein 2 heavy chain 1"
FT /id="PRO_0000318745"
FT REGION 1..1650
FT /note="Stem"
FT /evidence="ECO:0000250"
FT REGION 1651..1875
FT /note="AAA 1"
FT /evidence="ECO:0000250"
FT REGION 1941..2161
FT /note="AAA 2"
FT /evidence="ECO:0000250"
FT REGION 2249..2505
FT /note="AAA 3"
FT /evidence="ECO:0000250"
FT REGION 2617..2862
FT /note="AAA 4"
FT /evidence="ECO:0000250"
FT REGION 2880..3168
FT /note="Stalk"
FT /evidence="ECO:0000250"
FT REGION 3243..3472
FT /note="AAA 5"
FT /evidence="ECO:0000250"
FT REGION 3689..3904
FT /note="AAA 6"
FT /evidence="ECO:0000250"
FT COILED 669..696
FT /evidence="ECO:0000255"
FT COILED 2896..2981
FT /evidence="ECO:0000255"
FT COILED 3108..3199
FT /evidence="ECO:0000255"
FT COILED 3407..3441
FT /evidence="ECO:0000255"
FT BINDING 145..152
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT BINDING 1689..1696
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT BINDING 1979..1986
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT BINDING 2291..2298
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT BINDING 2655..2662
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT CONFLICT 1670
FT /note="K -> R (in Ref. 2; AAC52802)"
FT /evidence="ECO:0000305"
FT CONFLICT 1715
FT /note="C -> R (in Ref. 2; AAC52802)"
FT /evidence="ECO:0000305"
FT CONFLICT 1781
FT /note="V -> I (in Ref. 3; BAA05503)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 4306 AA; 492218 MW; A4AC9B7B9E7FA330 CRC64;
MAGSLSDVRK LFLFTTTQNY FGLRPELWDQ TPLSNCPEVN NFLDDGNQML LRVQRSDAGL
AFSNTIDFDD TKDKVLVFFK LRPEVITDGN LHTNILVSSM LESPINSLYQ AVRQVFAPML
LKDQEWSRNF DPKLQNLLSE LEAGLGVVLR KSDTNLPKLK LKEDDTRGIL TPSDEFQFWI
EQAHRGSKQI SKERASYFKE LFETIAREFY NLDSLSLLEV VDLVETTRDV VDDVWRQAEH
DHYPESRMLH LLDVIGGSFG RFVQKKLGSL KLWEDPYYLV KENLKAGISI CEQWVIVCSH
LTGQVWQRYV PHPWKSEKYF PETLDRLGKR LEEVLAIRTI HEKLLYFLPA SEERIVCLSR
VFEPFTGVNP VQYNPYTEPL WKAAVSQYEK IIAPAEQKIA GKLKNYISEI QDSPQQLLQA
FLKYKELVKR PTISKELMLE RETLLARLGD SAKDFRLDFE NRCRGIPGDA SGPLSGKNLS
EVVNNIVWVR QLELKVDDTI KIAEALLSDL SGFRSFHRSA EDLLDQFKLY EQEQFDDWSR
EVQSGLSDSR SGLCIEANSR IMELDPNDGA LKVHYSDRLV ILLREVRQLS ALGFVIPAKI
QQVANVAQKF CKQAIILKQV AHFYNSIDQQ MIQSQRPMML QSALAFEQII KNSKAGSGGK
SQITWDNPKE LEGYIQKLQN AAERLATENR RLRKWHITFC EKVVILMNID LLRQQQRWKD
GLQELRTGLA SVAAQGFQPS DMRAWRQHWN HQLYKALEHQ YQMGLEALNE NLPEINVDLT
YKQGRLQFRP PFEEIRAKYY REMKRFIGIP NQFKGVGGAG DESIFSVMID RNASGFLTIY
SKAEDLFRRL SAVLHQHKEW VVIGQVDMEA LVEKNLSTVH DWEKNFKALK IKGKEVERLP
SAVKVDCLNI NCSPVKTVID DLIQKLFDLL VLSLKKSIQT HIHEIDTFVT EAMKVLTVIP
QSVEEIGDTN LQYSNLQDRR PEILPLFQEA EDKNRLLRTV AGGGVETVSN LRAKWDKFEL
MMESHQLMIK DQIEVMKGNV KSRLQIYYQE LDKFKARWDQ LKPGDDIIET GQQNTMDQSA
KSIKEKKIEF DDLEVIRKKL VDDCHHFGLE ESNFSLAYSI SKDIESCAQI WALYEEFQQG
LQEMAKEDWI TYRTKIYIFE EFLINWHERL RKVEEHSVMT VKLQSEVDRY KIIIPILKYV
RGEHLSPDHW LDLFRLLGLP RGTSLEKLLF GDLLRVADTI VEKASDLKDL NSRAQGEVTI
REALRELDLW GVGAVFSLID YEDSQNRTIK LIKDWKDIVN QVGDNRCLLQ SLKDSPYYKG
FEDKVSIWER KLAQLDEYLQ NLNHIQRKWV YLEPIFGRGA LPKEQTRFNK VDEDFRSIMM
DIRKDSRVTT LTTHAGIRNT LLTILDQLQR CQKSLNEFLE EKRSAFPRFY FIGDDDLLEI
LGQSTNPSVI QSHLKKLFAG INSVCFDEES KHITAMRSLE GEVVPFKSKV LLSNNVEAWL
NDLALEMKQT LKQLLKECVT AGRSSQGAID PSLFPSQILC LAEQIKFTED VEDAIRDHSL
HQIEAQLAAK LERYTSVDTS SEDPGNSESG ILELKLKTLI LDIIHNIDIV KQLNQAQVHT
TDDWAWKKQV RFYMKSDHTC YVQMVDSELQ YTYEYQGNAP KLVYTPLTDK CYLTLTQAMK
MGLGGNPYGP AGTGKTESVK ALGGLLGRQV LVFNCDEGID VKSMGRIFVG LVKCGAWGCF
DEFNRLEEAV LSAVSMQIQT IQDALKNHRS VCELLGKEVE VNANSGIFIT MNPAGKGYGG
RQKLPDNLKQ LFRPVAMSRP DNDLIAEVIL YSEGFKDAKE LGRKLVAIFN LSRELLTPQQ
HYDWGLRALK TVLRGSGNLL RQLKKSGTKQ DVNENHIVVQ ALRLNTMSKF TFADCTRFDA
LIKDVFPGID FKEVEYNELS SALKQVFEEA NYEVIPNQMK KALELYEQLR QRTGVVIVGP
SGAGKSTLWR MLRAALCKIG KVVKQYTMNP KAMPRHQLLG HIDMDTREWS DGVLTNSARQ
VVREPQDVSS WIICDGDIDP EWIESLNSVL DDNRLLTMPS GERIQFGPNV NFVFETHDLS
CASPATISRM GMIFLSDEET DLNSLIKSWL RNQPVEYRSN LENWIGDYFS KALQWVLKQN
DYVVETSLVG TVMNGLSHLH GCKYHDQFII NLIRGLGGNL NMKSRLEFTK EVFNWARETP
PDSHRPMDTY FDCDRGQLAS YVLKKPESLT ADDFSSGHSL PVIQTPDMQR GLDYFKPWLS
SETKQPFILV GPEGCGKGML LRYAFSQLRS TEIATIHCSA QTTSRHLLQK LSQTCMVIST
NTGRVYRPKD CERLVLYLKD INLPKLDKWG TSTLVAFLQQ VLTYQGFYDE NLEWVGLENI
QIVASMSAGG RLGRHKLTTR FTSIVRLCAV DYPEREQLQT IYGAYLEAVL HKNLKNHSIW
GSSSKIYLLA GSMVQVYEQV RAKFTVDDYS HYFFTPCILT QWVLGLFRYD LEGGSSNHPL
DYVLEVVAYE ARRLFRDKIV GVKELHLFDN ILTSVLQGDW GSDILDNMAD SFYVTWGAQH
ISGAKIAPGQ PLPPHGKPLG KLSSADLKDV IKKGLIHYGR DNQNLDILLF QEVLEYMSRI
DRVLSFPGGS LLLAGRSGVG RRTVTSLVSH MHGAVLFSPK ISRGYEPKQF RTDLKHVLHL
AGIEAQQVVL LLEDYQFVHP TFLEMINSLL SSGEVPGLYT LEELEPLLLP LKDQASQDGF
FGPVFNYFTY RIQQNLHIVL IMDSANLNFI INCESNPALH KKCRVLWMEG WSDSSMKKIP
EMLFSEADIE EKYEKKRKDE KKKSSVDPDF IKSFLLIHES CKAYGATPSR YMTFLRVYSA
ISSSKRKELL KRQSHLQAGV SKLNEAKALV DELNRKAGEQ SILLRIKQDE ADSALQEITV
SMQDASEQKT ELERLKHRIA EEVVKIEERK SKIDDELKEV QPLVNEAKLA VGNIRPESLS
EIRSLRMPPD VIRDILEGVL RLMGIFDTSW VSMKSFLAKR GVREDIATFD ARNIPKEIRE
SVEELLFKNK ASFDPKNAKR ASTAAAPLAA WVKANVQYSH VLERIQPLET EQSGLELNLK
KTEDRKRKLE DLLNSVGQKV SELKEKFQSR TSEAAKLEAE VSKAQETIKA AEVLISQLDR
EHRRWNAQVA EIAEELATLP KRAQLAAAFI TYLSAAPEGL RKNCLEEWTK AAGLEKFDLR
RFLCTESEQL IWKSEGLPSD DLSIENALVI LQSRVCPFLI DPSSQATEWL KTHLKDSHLE
VINQQDSNFI TALELAVRFG KTLIIQEMDG VEPVLYPLLR RDLVAQGPRY VVQIGDKIID
YNEDFRLFLS TRNPNPFIPP DAASIVTEVN FTTTRSGLRG QLLALTIQHE KPDLEEQKTK
LLQQEEDKKI QLARLEESLL ETLATSQGNI LENKDLIESL NQTKASSALI QDSLKESYKL
QISLDRERDA YLPLAESASK MYFIISDLSK INNMYRFSLA SFLRLFQRAL QNKQDSESTE
QRIQCLVNSL KHMVYEYICR CLFKADQLMF ALHFVRGMHP EFFQENEWDT FTGVVVGDML
RKADSQQRIR DQLPSWIDQE RSWAVATLKI SLPGLYQTLC FEDGTLWRTY YHHSMCEQEF
PSILAKKVSL FQQVLVVQAL RPDRLQSAMA LFACKALGLK ELSPLPLNLK RLYKETLEIE
PILIIISPGA DPSQELQELA NAERSSERYH QVAMGQGQAD LAIQMLKECA RNGDWLCLKN
LHLVVSWLPV LEKELNTLQP KESFRLWLTA EVHPNFTPIL LQSSLKITYE SPPGLKKNLM
RTYESWTPEQ ISKKDNIHRA HALFSLAWFH AACQERRNFI PQGWTKFYEF SLSDLRAGYH
VIDRLFDGSK DVQWEFVHGL LENSIYGGRI DNYFDLRVLQ SYLKQFFNSS IIDVLNQRNK
KSIFPYSISL PDSCSILDYR AVIEKLPEDD KPSFFGLPAN IARSSQRMTS SQVISQLRIL
GRSVTAGCKF DREIWSNELS PVLNLWKKLN QNSNLIHQKV SPPNDRQGSP ILSFIILEQF
NAIRLVQSVH QSLAALSKVI RGTTLLSSEV QKLASALLNQ KCPLTWQSKW EGPEDPLQYL
RGLVARTLAI QNWVEKAEKQ ALLADTLDLS ELFHPDTFLN ALRQETARAT GCSVDSLKFV
ASWKGRLQEA KLQIKISGLL LEGCSFDGSR LSENQHDSPS VSSVLPCYMG WTPQGSYGPY
SPDECISLPV YTSAERDCVV TNIDVPCGGN QDQWIQCGAA LFLKNQ
