DYHC2_TRIGR
ID DYHC2_TRIGR Reviewed; 4318 AA.
AC Q27802;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 2.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=Cytoplasmic dynein 2 heavy chain 1;
DE AltName: Full=Dynein heavy chain isotype 1B;
GN Name=DYH1B;
OS Tripneustes gratilla (Hawaian sea urchin) (Echinus gratilla).
OC Eukaryota; Metazoa; Echinodermata; Eleutherozoa; Echinozoa; Echinoidea;
OC Euechinoidea; Echinacea; Temnopleuroida; Toxopneustidae; Tripneustes.
OX NCBI_TaxID=7673;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Embryo;
RX PubMed=8186465; DOI=10.1091/mbc.5.1.57;
RA Gibbons B.H., Asai D.J., Tang W.-J.Y., Hays T.S., Gibbons I.R.;
RT "Phylogeny and expression of axonemal and cytoplasmic dynein genes in sea
RT urchins.";
RL Mol. Biol. Cell 5:57-70(1994).
CC -!- FUNCTION: May function as a motor for intraflagellar retrograde
CC transport. Functions in cilia biogenesis (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: The cytoplasmic dynein complex 2 is probably composed by a
CC heavy chain DYH1B homodimer and a number of light intermediate chains.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, cilium axoneme
CC {ECO:0000250}. Cell membrane {ECO:0000250}; Peripheral membrane protein
CC {ECO:0000250}. Cytoplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the dynein heavy chain family. {ECO:0000305}.
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DR EMBL; U03969; AAA63583.2; -; mRNA.
DR PIR; T30297; T30297.
DR SMR; Q27802; -.
DR PRIDE; Q27802; -.
DR GO; GO:0005929; C:cilium; IEA:UniProtKB-KW.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0030286; C:dynein complex; IEA:UniProtKB-KW.
DR GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0045505; F:dynein intermediate chain binding; IEA:InterPro.
DR GO; GO:0051959; F:dynein light intermediate chain binding; IEA:InterPro.
DR GO; GO:0008569; F:minus-end-directed microtubule motor activity; IEA:InterPro.
DR GO; GO:0030030; P:cell projection organization; IEA:UniProtKB-KW.
DR GO; GO:0007018; P:microtubule-based movement; IEA:InterPro.
DR Gene3D; 1.10.8.710; -; 1.
DR Gene3D; 1.10.8.720; -; 1.
DR Gene3D; 1.20.140.100; -; 1.
DR Gene3D; 3.10.490.20; -; 1.
DR Gene3D; 3.20.180.20; -; 1.
DR Gene3D; 3.40.50.300; -; 5.
DR InterPro; IPR035699; AAA_6.
DR InterPro; IPR035706; AAA_9.
DR InterPro; IPR041658; AAA_lid_11.
DR InterPro; IPR042219; AAA_lid_11_sf.
DR InterPro; IPR026983; DHC_fam.
DR InterPro; IPR026815; DYNC2H1.
DR InterPro; IPR042222; Dynein_2_N.
DR InterPro; IPR043157; Dynein_AAA1S.
DR InterPro; IPR041228; Dynein_C.
DR InterPro; IPR043160; Dynein_C_barrel.
DR InterPro; IPR024743; Dynein_HC_stalk.
DR InterPro; IPR024317; Dynein_heavy_chain_D4_dom.
DR InterPro; IPR004273; Dynein_heavy_D6_P-loop.
DR InterPro; IPR013602; Dynein_heavy_linker.
DR InterPro; IPR013594; Dynein_heavy_tail.
DR InterPro; IPR042228; Dynein_linker_3.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR10676; PTHR10676; 1.
DR PANTHER; PTHR10676:SF352; PTHR10676:SF352; 1.
DR Pfam; PF12774; AAA_6; 1.
DR Pfam; PF12780; AAA_8; 1.
DR Pfam; PF12781; AAA_9; 1.
DR Pfam; PF18198; AAA_lid_11; 1.
DR Pfam; PF08385; DHC_N1; 1.
DR Pfam; PF08393; DHC_N2; 1.
DR Pfam; PF18199; Dynein_C; 1.
DR Pfam; PF03028; Dynein_heavy; 1.
DR Pfam; PF12777; MT; 1.
DR SUPFAM; SSF52540; SSF52540; 4.
PE 2: Evidence at transcript level;
KW ATP-binding; Cell membrane; Cell projection; Cilium;
KW Cilium biogenesis/degradation; Coiled coil; Cytoplasm; Cytoskeleton;
KW Developmental protein; Dynein; Membrane; Microtubule; Motor protein;
KW Nucleotide-binding.
FT CHAIN 1..4318
FT /note="Cytoplasmic dynein 2 heavy chain 1"
FT /id="PRO_0000318746"
FT REGION 1..1658
FT /note="Stem"
FT /evidence="ECO:0000250"
FT REGION 1659..1883
FT /note="AAA 1"
FT /evidence="ECO:0000250"
FT REGION 1951..2171
FT /note="AAA 2"
FT /evidence="ECO:0000250"
FT REGION 2261..2515
FT /note="AAA 3"
FT /evidence="ECO:0000250"
FT REGION 2623..2871
FT /note="AAA 4"
FT /evidence="ECO:0000250"
FT REGION 2888..3176
FT /note="Stalk"
FT /evidence="ECO:0000250"
FT REGION 3251..3487
FT /note="AAA 5"
FT /evidence="ECO:0000250"
FT REGION 3699..3914
FT /note="AAA 6"
FT /evidence="ECO:0000250"
FT COILED 1328..1354
FT /evidence="ECO:0000255"
FT COILED 1402..1431
FT /evidence="ECO:0000255"
FT COILED 1959..1986
FT /evidence="ECO:0000255"
FT COILED 2908..2989
FT /evidence="ECO:0000255"
FT COILED 3423..3480
FT /evidence="ECO:0000255"
FT BINDING 147..154
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT BINDING 1697..1704
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT BINDING 1989..1996
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT BINDING 2301..2308
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT BINDING 2661..2668
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
SQ SEQUENCE 4318 AA; 490483 MW; 5CCDBCE46D68D53D CRC64;
MPAEDARKEY LLSSCGSQLG LSASDDAITS LYSARELNTF LDDGNCSVLS AKLNSSGGNK
KVYLSNKVDP SDTGEKVIAF FKLRPDVISP DNVQSSILTV SMMDSPAGAL LHAVDKVFAP
LLIEDEKYNR SFNPKLQSLL SELRAGLKSL VRKQDPSQGG PVKRGDNDFA GILTPSDEFQ
HWSEVAMSGR NQDAKERAEH FLSFFETVSR EFDVLDTLSL EEAQELVETT QDAVDDVWKQ
GEFDPYPEKR MAHLLEVIGS AFGRFIQRKL GEFDLWLDPF HKVKQALLSG IAICEKWVSS
CEKLTEQFWK RDPMHPWNGA KFTPEGLSQL SKRLQEISTL RTIHEQLIRL LSKGEQEEVR
LKDVFSAFSG LNPLQYNPYT EPHWRAAVSQ YERAINPAER RIAIKLRSQF RNVEGNPNQL
LREFQKYKEL VSRESVRKEV VSERETLLGQ LQVYLKSIRD DFNVRTSESS PPTGKNLPEV
VNNIVWVRQL EAKVEQTSQV ADTLLNDLSG FGRFRRDAGD LLDELRNWRQ DQFDSWSSEM
QAAISDPNKP LSLQMTGRLM EFETKNNELI VSYSERLVTL MREVRQLQAL GYPVAAKIQH
TAAIAQKFYK HGVILKQVAH FHNSIAEQMI PSQRNMMLQS ALAFERIIKN PRTGSKDAGQ
GKVQVTWENP KELEEYITKL QAAAEKLTTE NRRLRKWHYT VCDKVVSLMS IDLLRQQPRW
KEGLQEIRGI MASLESQGYK SQDMKSWKMH WDRQLYKALE HQYQMGLEAL NENLPEIKVE
LTYRQQALQF RPPIEEIRAK YYREMKKFIS LPYHFRGIGD TGEPLIYPAI IERNASGFLN
VYKMAEVLFS KLEKAKDIFK NWVVLGSVDL DKLLETHLHT VTDWERNFKA LKARGRDSEK
LPSQVKVYCV TVSTAPVKGT IDDHIQRLFD SLLSSLRRSI TESVTEIEKF LKDGMEALAT
MPQTVEEIGN ANAKHAELEA KKPEMRPMFD RAEAKNKLLR TVAGGGVDQF TQLHAHWEKF
ELMMESHQLM IKEQVNVMKS NVESRVDAFR AELEKFSARW NHAKPSPEVI ESGDKESCLR
AVELVKEKKV EFDEMQKTKN SLIFDCKHFE LEEPFFSIAN DLEVDFDQQT TMWTLYEEFS
NGLSVLETED WIVFRSKTYQ FDDFLTTWTE KLKSGGPDVE HTIMTVRLSK DIDKYKTVSP
LLKYLSSDIF TQEHWMDLYR MLKMPRSKTV ERMTFGDILA TADHIVANAD GLKDLFNRAK
GEVSIREALR ELDVWGVGAC FSLTEYSDST DHQLMLIKDW KDLVTQVGDN QSLLQSLKDS
PYYKGFADKA TIWEQRLADL DEYLQNLNQI QRKWVYLEPI FGRGALPKEQ GRFRRVDDDF
RSIMMDVARD NRVLSLVGRS GLRTTLTTLL DQLQRCQKAL NEFLEEKRSI LPRFYFIGDD
DLLEILGQSQ NPAVIQSHLK KLFAGIHSVE FDQGCKHITA MKSLEGEVVP LLKAVEITPE
VEIWLGDLAK EMKSTLRKLL VDCLKDQQSS SSGVGVDPTK YPSQILGLAA QIQFTEQCET
AIRDNSLQDL LIELEQQLEG YTSADISGAA GERNAAIHVL ELKLKALILD TIHSLDVVQL
LQKENVTSLD NWLWQKQLRY YLDNTKTAIM RMVDAEFQYT YEYQGNAAKL VHTPLTDKCY
LTLTQGMHMG MGGNPYGPAG TGKTESVKAL GGLFGRQVLV FNCDEGVDVK SMGRIFIGLV
KCGAWGCFDE FNRLEEAVLS AVSMQIQTIQ AALKQKKPKV DLLDRTIDID PNSGIFITLN
PAGKGYGGRQ KLPDNLKHVF RPVAMSRPDN EQIAEVILFS EGFKDGKSLG RKLVAIFNLA
KELLTPQQHY DWGLRALKTV LRGCGNLLQI ARQQAGQDRS KVQEPKLVVQ ALRVNTLSKL
TFSDGIRFDA LVKDVFPGVE LKDIEYMTLA DAIRQHCKEH NLVVMETQVK KALELYEQLR
QRMGVVVVGP SGSGKSTTWQ ILRAALNNTG QVVKQYTMNP KAMPRTQLLG HIDMDTREWS
DGVLTYSARQ VVREPQEIHS WIICDGDIDP EWIESLNSVL DDNRLLTMPS GERIQFGPNV
NFLFETHDLS CASPATISRM GMIFMSDEDT EIKDLVQSWL SHQAEESRNR LAAWIEDHFY
RALEWVMRQG DFVVDTSLVG VVMNGLSHLR GAECISDFTI KLIRGLGANL PEATRMNFAK
EVFHMTREQP PDPRRILDTY FDERTGSLAT YTMQDNDELS ASDFNNPSSL PVIRTPDVQR
CLDFFNPWLE ADNRQPFILV GPDGCGKGMV LRHCFAQLRS TQIATIHCNA QTSPTHLLQK
LQQMCMVLST NTGRVYRPKD CENLVLYLKD LNLPKPDKWG TCQLLAFLQQ VLTYHGFYDD
KLEWVGLDGV QIVASMNAAH TVGRHPLSTR FTSIVRICSI GYADREQLQG IYSAYLRPVL
HRSLGNHPVW SSPSKVSMLA GSMISIYEQV RSKFTIDDHS HYLFTPRDLT QWVLGLLRYD
LGGSSESTSE HVLEVLSYEA RRLFCDRLVG EEARNRFDNI LNGTLQADWN AGQILQNLNG
HYYVTWGART ETSSGGSLPP AGKSLGRLSA SDLKEVIKKA KKTFARENRE LDIQIFHEVL
DHVARVDRVL TQPRGSLLLA GRSGVGRRTA ASLVAHCHRT ELFSPNLSRA YGLKQFKNDL
KTAMQQAGVE GNQVVLLLED HQFIQPQFLE LINSLLSSGE IPGLYSPEEL EPLLAPIRDQ
ASEEGFRGTL ISYYASRILT NLHVVLIMDS KNASFAVNCQ SNPAFYKSCS VQWMEGWSKE
SMKEIPRLLL RHHKGDTKDE GSKEDRKRHR KVSGGDEIIR NFLEIHKSSS VRHSTPRRYM
TFLHTYLDVY RRKKQGVEEK QKHLQAGVAK LNEAKALVAE LNSKAAKQSA LLAEKQKEAD
EALKKIQTSM EKAGEQRREI EILKQQADEE NVKLEKRKRV IDEELAEIEP QVQAAKSAVG
SIKSESLSEI RAMRAPPDVI RDILEGVLRL MGIFDTSWVS MKSFLAKRGV KEEISTFDAR
KINKDLREGT EQLLKKHASS FDPQNAKRAS VAAAPLAAWV KANVKYSYAL EKIEPLETEQ
NQLKKNLEKA VGRIEKLSKG LADVDHRVDE YKRRFQKLNE EAAKLKYELE KEQETIASAE
NLIGKLEGEY QRWNNQVSEL NTELETLPKK AQCAAGFITY LTSSPEDERK QKLAEWSKLC
GLERFDMRRF LSTESEQLTW KGEGLPSDEL SVENAVMILQ TNDMSIKSSL RPFLIDPSLR
ATEWLKTHLK EARLEVINQQ DANFSTALEL AVRFGKTLII QEMDKIEPVL YPLLRADLIS
QGPRFVVQIG DKVIDYHEDF RLFMTTRNPN PEIPPDAASI ISEINFTTTR AGLTGQLLAA
TIQHEKPELE VRKTELLKQE EDLKIQLAQL EESLLETLAK AEGNILENKA LLESLNKTKE
SSQTITQSLV ESVQLQESLD QERAAYLPLA ENGSALFFVI SDLAKLNNMY RFSLGAFLRL
FSKALQGRMD GSSTEMRIQK LIKSLQMLVY EYVCQSLFKA DRLMFALHLV HGMHPNHFKE
NEWEAFLGQI VADVRDSQQS GSMPSWVDQD RHKALASFMA TFPSVGQSLS LTDNGLWGHF
NRSSQCEHEF PTSLEKKISP FQQLLLIQAI RPDRLQSAMT FFAQRGLGMK ELTTPTINLK
RLCQDTVPSE PILIIISPGA DPTQELQEVA AEVIGADHYH EVAMGQGQAD IAMQLLHECS
RNGEWLCLKN LHLVTAWLPV LEKEMNALSP HENFRLWLTA ESHPKFPTIL LQSSLKITYE
APPGIKKNLL RSYDSWTPEF IGRENNVVRA QALFALAWFH AICQERRNFI PQGWTKFYEF
SMSDLRAGAD IIDRLCHGKG KEVQFEFIYG LFENAIYGGR VDNPFDMRVL RSYLAQYFNK
EMLGGSSARR SKKLPGGNSI PVSSYYKDFT ELVSKFPDDD KPSFFGLPAN IERSSQRIIS
VQVISQLKIL RRSDEAAEKF DTEKWNTELG PILSLWKKLN QGSNLIQTKA QPPSDRSGTQ
GPIDSFIQLE RYNALQLVQF VHSTLAALSK VIRGTSLLTS EVQALASNLL KQETPLSWQS
KWEGPEDPVL YLKTIVSRTM AIQGWVEKAQ RNNLLSDTLN LSELFHPDTF LNALRQQTAR
DSKTSMDSLK FACRWSGNIS GAKYQVKIGG LHLEGCTFDG QRLSENQRDS PSVCSVPACS
VAWVLKDTPN TYHASESISL PVYFTEQREK IVTHLVLPCG GEQDHWIQTG AALFLQNQ
