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DYHC_ASHGO
ID   DYHC_ASHGO              Reviewed;        4083 AA.
AC   Q9C1M7;
DT   31-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   03-AUG-2022, entry version 118.
DE   RecName: Full=Dynein heavy chain, cytoplasmic;
DE   AltName: Full=Dynein heavy chain, cytosolic;
DE            Short=DYHC;
GN   Name=DYN1; Synonyms=DHC1; OrderedLocusNames=ACR258W;
OS   Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS   (Yeast) (Eremothecium gossypii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX   NCBI_TaxID=284811;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=11181180; DOI=10.1242/jcs.114.5.975;
RA   Alberti-Segui C., Dietrich F.S., Altmann-Joehl R., Hoepfner D.,
RA   Philippsen P.;
RT   "Cytoplasmic dynein is required to oppose the force that moves nuclei
RT   towards the hyphal tip in the filamentous ascomycete Ashbya gossypii.";
RL   J. Cell Sci. 114:975-986(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=15001715; DOI=10.1126/science.1095781;
RA   Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA   Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA   Gaffney T.D., Philippsen P.;
RT   "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT   cerevisiae genome.";
RL   Science 304:304-307(2004).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=23749448; DOI=10.1534/g3.112.002881;
RA   Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT   "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT   loci, numerous translocations, lack of transposons, and distinct gene
RT   duplications.";
RL   G3 (Bethesda) 3:1225-1239(2013).
CC   -!- FUNCTION: Cytoplasmic dynein acts as a motor for the intracellular
CC       retrograde motility of vesicles and organelles along microtubules.
CC       Dynein has ATPase activity; the force-producing power stroke is thought
CC       to occur on release of ADP. Required to maintain uniform nuclear
CC       distribution in hyphae. May play an important role in the proper
CC       orientation of the mitotic spindle into the budding daughter cell
CC       yeast. Probably required for normal progression of the cell cycle.
CC   -!- SUBUNIT: Consists of at least two heavy chains and a number of
CC       intermediate and light chains. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton {ECO:0000250}.
CC       Note=Probably binds indirectly to the inner plasma membrane.
CC       {ECO:0000250}.
CC   -!- DOMAIN: Dynein heavy chains probably consist of an N-terminal stem
CC       (which binds cargo and interacts with other dynein components), and the
CC       head or motor domain. The motor contains six tandemly-linked AAA
CC       domains in the head, which form a ring. A stalk-like structure (formed
CC       by two of the coiled coil domains) protrudes between AAA 4 and AAA 5
CC       and terminates in a microtubule-binding site. A seventh domain may also
CC       contribute to this ring; it is not clear whether the N-terminus or the
CC       C-terminus forms this extra domain. There are four well-conserved and
CC       two non-conserved ATPase sites, one per AAA domain. Probably only one
CC       of these (within AAA 1) actually hydrolyzes ATP, the others may serve a
CC       regulatory function.
CC   -!- SIMILARITY: Belongs to the dynein heavy chain family. {ECO:0000305}.
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DR   EMBL; AF287477; AAK20175.1; -; Genomic_DNA.
DR   EMBL; AE016816; AAS51484.1; -; Genomic_DNA.
DR   RefSeq; NP_983660.1; NM_209013.1.
DR   SMR; Q9C1M7; -.
DR   STRING; 33169.AAS51484; -.
DR   PRIDE; Q9C1M7; -.
DR   EnsemblFungi; AAS51484; AAS51484; AGOS_ACR258W.
DR   GeneID; 4619795; -.
DR   KEGG; ago:AGOS_ACR258W; -.
DR   eggNOG; KOG3595; Eukaryota.
DR   HOGENOM; CLU_000038_7_0_1; -.
DR   InParanoid; Q9C1M7; -.
DR   OMA; FIMDEAN; -.
DR   Proteomes; UP000000591; Chromosome III.
DR   GO; GO:0000235; C:astral microtubule; IEA:EnsemblFungi.
DR   GO; GO:0005938; C:cell cortex; IBA:GO_Central.
DR   GO; GO:0005868; C:cytoplasmic dynein complex; IBA:GO_Central.
DR   GO; GO:0005881; C:cytoplasmic microtubule; IBA:GO_Central.
DR   GO; GO:0030286; C:dynein complex; IBA:GO_Central.
DR   GO; GO:0005816; C:spindle pole body; IEA:EnsemblFungi.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0045505; F:dynein intermediate chain binding; IBA:GO_Central.
DR   GO; GO:0051959; F:dynein light intermediate chain binding; IBA:GO_Central.
DR   GO; GO:0008569; F:minus-end-directed microtubule motor activity; IBA:GO_Central.
DR   GO; GO:0031122; P:cytoplasmic microtubule organization; IBA:GO_Central.
DR   GO; GO:0000132; P:establishment of mitotic spindle orientation; IEA:EnsemblFungi.
DR   GO; GO:0051293; P:establishment of spindle localization; IBA:GO_Central.
DR   GO; GO:0000741; P:karyogamy; IEA:UniProtKB-KW.
DR   GO; GO:0007018; P:microtubule-based movement; IBA:GO_Central.
DR   GO; GO:0072382; P:minus-end-directed vesicle transport along microtubule; IBA:GO_Central.
DR   GO; GO:0000278; P:mitotic cell cycle; IBA:GO_Central.
DR   GO; GO:0000070; P:mitotic sister chromatid segregation; IEA:EnsemblFungi.
DR   GO; GO:0007097; P:nuclear migration; IBA:GO_Central.
DR   GO; GO:0030473; P:nuclear migration along microtubule; IEA:EnsemblFungi.
DR   Gene3D; 1.10.8.710; -; 1.
DR   Gene3D; 1.10.8.720; -; 1.
DR   Gene3D; 1.20.140.100; -; 1.
DR   Gene3D; 3.20.180.20; -; 1.
DR   Gene3D; 3.40.50.300; -; 5.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR035699; AAA_6.
DR   InterPro; IPR035706; AAA_9.
DR   InterPro; IPR041658; AAA_lid_11.
DR   InterPro; IPR042219; AAA_lid_11_sf.
DR   InterPro; IPR026983; DHC_fam.
DR   InterPro; IPR042222; Dynein_2_N.
DR   InterPro; IPR043157; Dynein_AAA1S.
DR   InterPro; IPR041466; Dynein_AAA5_ext.
DR   InterPro; IPR024743; Dynein_HC_stalk.
DR   InterPro; IPR024317; Dynein_heavy_chain_D4_dom.
DR   InterPro; IPR004273; Dynein_heavy_D6_P-loop.
DR   InterPro; IPR013602; Dynein_heavy_linker.
DR   InterPro; IPR013594; Dynein_heavy_tail.
DR   InterPro; IPR042228; Dynein_linker_3.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR10676; PTHR10676; 1.
DR   Pfam; PF12774; AAA_6; 1.
DR   Pfam; PF12780; AAA_8; 1.
DR   Pfam; PF12781; AAA_9; 1.
DR   Pfam; PF18198; AAA_lid_11; 1.
DR   Pfam; PF08385; DHC_N1; 1.
DR   Pfam; PF08393; DHC_N2; 1.
DR   Pfam; PF17852; Dynein_AAA_lid; 1.
DR   Pfam; PF03028; Dynein_heavy; 1.
DR   Pfam; PF12777; MT; 1.
DR   SMART; SM00382; AAA; 3.
DR   SUPFAM; SSF52540; SSF52540; 4.
PE   3: Inferred from homology;
KW   ATP-binding; Coiled coil; Cytoplasm; Cytoskeleton; Dynein; Karyogamy;
KW   Microtubule; Motor protein; Nucleotide-binding; Reference proteome; Repeat.
FT   CHAIN           1..4083
FT                   /note="Dynein heavy chain, cytoplasmic"
FT                   /id="PRO_0000114637"
FT   REGION          1..1745
FT                   /note="Stem"
FT                   /evidence="ECO:0000250"
FT   REGION          1746..1967
FT                   /note="AAA 1"
FT                   /evidence="ECO:0000250"
FT   REGION          2026..2265
FT                   /note="AAA 2"
FT                   /evidence="ECO:0000250"
FT   REGION          2373..2622
FT                   /note="AAA 3"
FT                   /evidence="ECO:0000250"
FT   REGION          2716..2980
FT                   /note="AAA 4"
FT                   /evidence="ECO:0000250"
FT   REGION          2987..3294
FT                   /note="Stalk"
FT                   /evidence="ECO:0000250"
FT   REGION          3364..3592
FT                   /note="AAA 5"
FT                   /evidence="ECO:0000250"
FT   REGION          3748..3952
FT                   /note="AAA 6"
FT                   /evidence="ECO:0000250"
FT   COILED          127..166
FT                   /evidence="ECO:0000255"
FT   COILED          381..402
FT                   /evidence="ECO:0000255"
FT   COILED          801..821
FT                   /evidence="ECO:0000255"
FT   COILED          3015..3085
FT                   /evidence="ECO:0000255"
FT   COILED          3223..3302
FT                   /evidence="ECO:0000255"
FT   COILED          3527..3607
FT                   /evidence="ECO:0000255"
FT   BINDING         1784..1791
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
FT   BINDING         2064..2071
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
FT   BINDING         2412..2419
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
FT   BINDING         2754..2761
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   4083 AA;  466252 MW;  09DD11D4EF1225E1 CRC64;
     MTDDQVAQAL VGYVFNVAKL FLKLGAEQDA FARTHGAKFE EWVGNGNMRT LFLVKDEEGE
     VQVLEELEGP EAEGPEQSGR LLLIKNRPFV DGSAPMESQV QVLHLPPRAH FDGFKSFVSF
     GVATMFDAVV STNSNLEAKQ ESINSARRKI KDLSLSLQSL QQFIEVPDIS ATAHPLIKKI
     IAEGANPRNY TTYISDEQFA DSQFLNSLQK IANGWIKSAQ NLTKLTRNIE DGSATDEIRF
     WINLEQSLLA LEKQIAIPEV EITLSILTAA KRFHATVTFI SDTGLRDRIL ETQSYNQIMS
     DFPLADLQTA TSFTKLGEAI ESISIALKKL KVSTYPLARA VTFVEKISTE LDQKLREMLP
     NLISSDFISF QEDYDHCIKI INQWEALLKE FTSLIRELMR KRSEKFIFMK IDTQTDSLKE
     VLNTVAAFRK KHDILIHVLK GIGYDTLSED IQSIYEPVQY QDPLRDNASK WANAEAAYNQ
     RVSLLENKLV DMLKKKLDDC KSSDSMFSIF EKYRPLMKRP RIQGAVREYQ HELLHNVKDD
     LEHIHQQLSL QKWNSELSRL NDIPPVSASI IWSKQLTKKL QNLTSRLGLI LGEDWISTSE
     GSQIFVECSS IMKVLNTDKL FETWVSNVSS QNFLLDEPIF KILITNEEYE LHVNFDSVVG
     SLFKEVRNLM WMGFNVPSNI IKNSRRVRSL YPHAVMVSEL LQTFVSAVQS FQERPHTWLL
     LKDETENIWQ LLSAMITDTW DSVPLFEDDA SHERAEDIQR DEPSILRLEH SIGELLSKFQ
     QLDGLEKGLS SCLQQLEVFG KLDLQNLEVL INKIQLLVDQ ATLHGFHNMS GFIDYLNTRI
     RSYLVSTVSK ILEESQLSPK KHYILQQGKK VTISPSIEET KKAWMRDFQK TLEVATNLPK
     ITDKKFDITE GQMENFTDIG TDLSESLIKA FLRIEDACHA IDEHFQKWKK LELLWCLDEL
     TLLERLGSDV EVSYRFLLDF MEERKAIDMV DSEITIAGDT MINNEQVYVR VSAKYDNWQR
     VLCEKLLENY MDHASEFDTQ LVHSRRLLET SIINLGSLSK TTELIAYVDD IKNNLDLMFT
     RYSLLLNTQK LLQKLRFRIP QNFIHAEQIE SDLVSLREIC LRKEDLINKN RDAISNKLEA
     ELLKIQEVAN SLSQSWSKKK PLSVSIQPSE ALSVLNTFED SIAKVNTERE LINRAAKILL
     VPIKLQNVLS PVIEEVNDYK AVWSSVDGLW NSFNATLSVK WADFESTAVK HRLEALMKKC
     QDMPPKVLQY KIFQNIAGSI EATLKSMHLL KALKEPAIKP RHWSILFKQL GASHIVSGNI
     DDQTFTLEDI LQLNILLNEV SVKKIVIKAR NENVLESSLS QMKARWRATK FDQFVHSSGL
     VLVKGWDVIF SNCNDDLNMI TSMKNSPYFK VFEQEALEWE TKLSNFYDIV LSWVEVQRQW
     MYLFGILAKK TEMKNLLPIE ASKFASLTSE YNSLLLKLYG SEIAIDILHV HSTLPTLKRM
     AESLTKIRKS LNDFLETQRR LFPRFYFVGN EDLLQIIGAG DNFSEFSRHL SKLFSSVSDF
     IYDESLIQGV YSLEGETLLF ANPVRVTPSS KLDQWMNEVD LEIKLTLSTL VKNCLESYRT
     SGSLKHIIEK YPFQALLLAL QCTWTNKIET SMTKDNFGSI CSSIDEEMAS LAAVIDSYPT
     VTEKRKVESL IVELVHLKTI TETLKNVELE QIDFHWKQTQ RFYWDDNSND PLNSITIEQS
     CVSFCYGFEY IGVPERLIYT PLLDSCFNAM VLALSEHMGG CPFGPAGTGK TETIKALGQN
     FGRMVLVFNC DDSFDFQAMS RLLFGITQVG AWGCFDEFNR LEEKILSAVS TQVEAIQLSL
     VQGKPEIEVL DKKGSLNSNT GIFITMNPGY AGRSELPENL KKMFREFAMM KPDALVIAEV
     ILTILGLENP RVLAEKIVSL FKLLNDKTTS QKHYDFGLRA LKSVLRNCLT ILRSTTDLDS
     TQVLLRSLNE MVVPKLISVD EAVYEEAIAD FFPGSRIKPS NEQLLSYLAS YCESNQLVAS
     DLFIKKCSQF YDIQKTQQAI ILAGDAGTGK TSVWKSVINS MKRSGAKENI VYIIDTKTLK
     KEDLYGKLDP VTFDWKDGIF THLLRKTLLD TMGNFKNSNI WIVFDSDLDP NYTETLNSVL
     DDNKVLTLPN GERLKIPPNL HILFEVQDLE HATAATVSRC GMIWFANNTL AAQDILISCL
     SREVATLQQD ADVHDNIIAT IQDIFAQFIQ GSTLGNVIEA TYKADHIMGV DFCRFIETAV
     TLLSCDIKKN KKQLSRLSQV ACVRYMSKRL ALVLIWAFVG GSDLETREKF SETICELLGI
     SDIPTGSKFL LDYDVSVATQ DWVPVSAEVP KTSLESHEVL IPDLIIPTVD TVRHETLLFD
     LLNADRPLIL CGPPGSGKTM TLYNTLKRSD RFNIIGINFS KDTSVELFLK TLEQHTICTP
     TSRGIIMQPK AHGKQLVVFC DEINLPMLDE YGSQPVILFL RQLIEKRGFW NVQESKWVFI
     ERIQIVGACN PPGHAGRVSI TPRFLRHASI VMVDYPGQIA MEQIYETFFN AIFKLTPKLK
     GFASDFTKAS LQVYYDCKAT YTSEAHSHYI YSPRELTRWV RGIHFTISDS GNIDLAYMLE
     LWAHESLRLF SDRLVSSSEK NIFQSILQNA ITTHFPNQPL GSLESSQLLF SNWLSLNYSK
     VVKSEMYTFI KERLKTFAEE ELDTELTIYD DMIDNILRID RILKQVQGHG ILVGPNYSGK
     TTITRFVAWM NGIKVVRPTI HRHFTIENFD EFLKQMLLRC GTESEKICLI IDESNILETS
     FLERMNTLLA NSDVPGLFEA DEYEALLSKI GQRISQLGLL LDTEQEMYDW FTSEISKNLH
     VIFNINDPDN RESTQLITSP ALFNRSVINW IGTWSSRSCL HVVNEVIKNM PLDRADYTIP
     HHAAANLIVP DGNLVTIRDV VANLFVLFHE QYHRLLGNSQ GSPSAFLTSL RRFQSLYMSK
     LKELEEHQRF TLVGLEKLKD TVIKVKQLNQ SLSQKQVELQ QKEKEARDTL DKMLVDQNEA
     ERKQEASVEI QKILALQEKE INERRKIIMA DLAVAEPAIL EAQRGVKNIK KQQFTELRSM
     LNPPDAVKTT LEAVCVILGY SCKTWKDIQL AIRKDEFVTD IVYYNTETMM TPAMKQDIET
     DYLSRPKFNY ESVNRASLAC GPLYQWIVAQ ISYSEMLVKV TPLKEEMVKV ENEMLQNKAR
     LMAAGEMIKE LQTSIESSKV SYSKLIREVE ITKTEMESVQ SKVERSIKLM ESLTGEKERW
     IKNTEHFKDW NKNLIGNCFL SSLYESYCGP HDQSLRLKLF TIWSNTLAKF GIEYEPTYSF
     ITDMVNPLTK VNWVACGLPD NELFVANFHI AMNSCHYPYV IDPSSTIVDT FANFYGRKMM
     ITSFLDVGFV KQLENALRFG GCILIQDGEF FDPIISHLIA KEFKKAGGRL TVQIGDHEVD
     VSTSFQLIIH SKDPNSYMSS FVKTRMAVIN FTVSKGSIEA QALQITLEKE NPELQKQRTD
     LLKLNGEYKL HLRSLEDKLL ESLNESDGSI LENDSLISTL EQLKIESSEI AKKIEETNTV
     IVKVEDLVNE YNVLGEQSVL IFNLLESITQ WHWFYQIPIE QFMECFSSIF ATKTRENMTR
     SEHLLLALYE HVYMWFSHVF KDRDRMAFGI LLFASYHHSR ESKFFSEHFW KIIEGIASDT
     LGTVEHITDT KLEQLVAAAN EKDYLKGLKS LLEFLPESSW HDSVPKYQNI IVACERGVDG
     TFKVQQLAQE MGKTVHSVAL GSAESISMAE QDLIQYSGEG KWLLLQNLQM SHEWANTVLP
     KKLESIKANP DFRVFMTCGI QSKPLVVPLL SRSYKIAYEG EPGVLNTVCE LWRTQSEELK
     NVKPVEKLHS KFILVWFHSI IMARCRLAPI GFTKKYDFHD GDFHAGSKFL DHIFEQSSNG
     KEHVDPDLVP WKLVSDTIGK IIYGGKVDDP ADLDWCKRSA RRMFSSDAYL NNFEVVQGLT
     VPIDRSSYSQ YDKWFKSLDA AAERTTAWLE LSDASALQNF YAHEARMICK KIIQTNGPTS
     LIH
 
 
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