DYHC_HELCR
ID DYHC_HELCR Reviewed; 4466 AA.
AC P39057;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 88.
DE RecName: Full=Dynein beta chain, ciliary;
OS Heliocidaris crassispina (Sea urchin) (Anthocidaris crassispina).
OC Eukaryota; Metazoa; Echinodermata; Eleutherozoa; Echinozoa; Echinoidea;
OC Euechinoidea; Echinacea; Camarodonta; Echinidea; Echinometridae;
OC Heliocidaris.
OX NCBI_TaxID=1043166;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 1192-1204 AND
RP 3324-3340.
RC TISSUE=Egg, and Sperm;
RX PubMed=1830928; DOI=10.1038/352643a0;
RA Ogawa K.;
RT "Four ATP-binding sites in the midregion of the beta heavy chain of
RT dynein.";
RL Nature 352:643-645(1991).
CC -!- FUNCTION: Force generating protein of eukaryotic cilia and flagella.
CC Produces force towards the minus ends of microtubules. Dynein has
CC ATPase activity; the force-producing power stroke is thought to occur
CC on release of ADP.
CC -!- SUBUNIT: Consists of at least two heavy chains (alpha and beta), three
CC intermediate chains and several light chains.
CC -!- SUBCELLULAR LOCATION: Cell projection, cilium, flagellum. Cytoplasm,
CC cytoskeleton, flagellum axoneme.
CC -!- DOMAIN: Dynein heavy chains probably consist of an N-terminal stem
CC (which binds cargo and interacts with other dynein components), and the
CC head or motor domain. The motor contains six tandemly-linked AAA
CC domains in the head, which form a ring. A stalk-like structure (formed
CC by two of the coiled coil domains) protrudes between AAA 4 and AAA 5
CC and terminates in a microtubule-binding site. A seventh domain may also
CC contribute to this ring; it is not clear whether the N-terminus or the
CC C-terminus forms this extra domain. There are four well-conserved and
CC two non-conserved ATPase sites, one per AAA domain. Probably only one
CC of these (within AAA 1) actually hydrolyzes ATP, the others may serve a
CC regulatory function.
CC -!- SIMILARITY: Belongs to the dynein heavy chain family. {ECO:0000305}.
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DR EMBL; D01021; BAA00827.1; -; mRNA.
DR PIR; S17231; S17231.
DR SMR; P39057; -.
DR PRIDE; P39057; -.
DR GO; GO:0097729; C:9+2 motile cilium; IEA:UniProt.
DR GO; GO:0005858; C:axonemal dynein complex; IEA:InterPro.
DR GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0045505; F:dynein intermediate chain binding; IEA:InterPro.
DR GO; GO:0051959; F:dynein light intermediate chain binding; IEA:InterPro.
DR GO; GO:0008569; F:minus-end-directed microtubule motor activity; IEA:InterPro.
DR GO; GO:0030030; P:cell projection organization; IEA:UniProtKB-KW.
DR GO; GO:0003341; P:cilium movement; IEA:InterPro.
DR Gene3D; 1.10.8.710; -; 1.
DR Gene3D; 1.10.8.720; -; 1.
DR Gene3D; 1.20.140.100; -; 1.
DR Gene3D; 3.10.490.20; -; 1.
DR Gene3D; 3.20.180.20; -; 1.
DR Gene3D; 3.40.50.300; -; 5.
DR InterPro; IPR035699; AAA_6.
DR InterPro; IPR035706; AAA_9.
DR InterPro; IPR041658; AAA_lid_11.
DR InterPro; IPR042219; AAA_lid_11_sf.
DR InterPro; IPR026983; DHC_fam.
DR InterPro; IPR041589; DNAH3_AAA_lid_1.
DR InterPro; IPR030443; DNAH9-like.
DR InterPro; IPR042222; Dynein_2_N.
DR InterPro; IPR043157; Dynein_AAA1S.
DR InterPro; IPR041466; Dynein_AAA5_ext.
DR InterPro; IPR041228; Dynein_C.
DR InterPro; IPR043160; Dynein_C_barrel.
DR InterPro; IPR024743; Dynein_HC_stalk.
DR InterPro; IPR024317; Dynein_heavy_chain_D4_dom.
DR InterPro; IPR004273; Dynein_heavy_D6_P-loop.
DR InterPro; IPR013602; Dynein_heavy_linker.
DR InterPro; IPR013594; Dynein_heavy_tail.
DR InterPro; IPR042228; Dynein_linker_3.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR10676; PTHR10676; 1.
DR PANTHER; PTHR10676:SF257; PTHR10676:SF257; 1.
DR Pfam; PF12774; AAA_6; 1.
DR Pfam; PF12780; AAA_8; 1.
DR Pfam; PF12781; AAA_9; 1.
DR Pfam; PF17857; AAA_lid_1; 1.
DR Pfam; PF18198; AAA_lid_11; 1.
DR Pfam; PF08385; DHC_N1; 1.
DR Pfam; PF08393; DHC_N2; 1.
DR Pfam; PF17852; Dynein_AAA_lid; 1.
DR Pfam; PF18199; Dynein_C; 1.
DR Pfam; PF03028; Dynein_heavy; 1.
DR Pfam; PF12777; MT; 1.
DR SUPFAM; SSF52540; SSF52540; 4.
PE 1: Evidence at protein level;
KW ATP-binding; Cell projection; Cilium; Cilium biogenesis/degradation;
KW Coiled coil; Cytoplasm; Cytoskeleton; Direct protein sequencing; Dynein;
KW Flagellum; Microtubule; Motor protein; Nucleotide-binding; Repeat.
FT CHAIN 1..4466
FT /note="Dynein beta chain, ciliary"
FT /id="PRO_0000114644"
FT REGION 1..1813
FT /note="Stem"
FT /evidence="ECO:0000250"
FT REGION 1814..2035
FT /note="AAA 1"
FT /evidence="ECO:0000250"
FT REGION 2095..2316
FT /note="AAA 2"
FT /evidence="ECO:0000250"
FT REGION 2422..2669
FT /note="AAA 3"
FT /evidence="ECO:0000250"
FT REGION 2767..3016
FT /note="AAA 4"
FT /evidence="ECO:0000250"
FT REGION 3033..3325
FT /note="Stalk"
FT /evidence="ECO:0000250"
FT REGION 3409..3636
FT /note="AAA 5"
FT /evidence="ECO:0000250"
FT REGION 3846..4072
FT /note="AAA 6"
FT /evidence="ECO:0000250"
FT COILED 482..502
FT /evidence="ECO:0000255"
FT COILED 627..643
FT /evidence="ECO:0000255"
FT COILED 734..805
FT /evidence="ECO:0000255"
FT COILED 1036..1056
FT /evidence="ECO:0000255"
FT COILED 1306..1337
FT /evidence="ECO:0000255"
FT COILED 1443..1468
FT /evidence="ECO:0000255"
FT COILED 3033..3134
FT /evidence="ECO:0000255"
FT COILED 3263..3325
FT /evidence="ECO:0000255"
FT COILED 3573..3642
FT /evidence="ECO:0000255"
FT BINDING 154..161
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT BINDING 1852..1859
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT BINDING 2133..2140
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT BINDING 2460..2467
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT BINDING 2805..2812
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
SQ SEQUENCE 4466 AA; 511783 MW; C465CC5C6C6D325D CRC64;
MGDVVDARLD FISEYILKSY KLKPDKWTKC INVEENKILM LEFLEKADNP QLVFTVNPAG
LITPSYEFPS ALKNTKAIYF IKKGREPVGK DNIKTNLVYG DLSYTPLEQL SALVDEVLVP
LLANPRNHEQ WPVVVSQDVL RHVHNLKSSV YVVAGQVKGK TLLPLPVGSE KVETAAGSEE
KDDSYDRSLV HAIESVIIDW THQIRDVLKR DSAQPLLEGL NPGPMVEINF WKAKCENLDC
IFQQLRDPKV RKMKELLERT QSSYLPSFNN IERDVEAALT EAQDINIHLK PLVYQIESMD
ELEFSDLTPR LAPILHTVCL IWSNSDYYNT APRVIVLLQE ICNLLIDLCR TFLDPSEIFK
LEPEESLEKV RGALTVLKNW RELYDEHRAK LKDYFKDGKE VKEWEFASPL VFTRMDNFIR
RIETIQSLFE TNVEFSKLEK TEMGSMKGRM LSQQVEKIHE EFQECAKVFT ERPYDGLDPT
CQEFLEDYEE FEKKVFDLDR RLGSILCQGF DDCCGLEAAF KMLDCYGPLL DRPVIRNDFE
CKYPIVLMLY DQELDQSKEI YDEHMRVEEA NGNAPLNKNM PDVAGQLKWS AQLRDRISKP
MGSLKHMEHP TGVRRILESE DAKVIFQKYE EMLNLLNKYE QKVFENWTKG VDEVCKTNLD
QSLITRDDAS KLIMVNFDPK LVSVLREVKY LQIRGEETIP ESAASIYEKH ETLRKYVANL
DLTVAWYNKV RKTVLEVEFP LIEGQLADLD TRLRQAEADL NWTSDSVWEY IQETRDQVRD
LEKRVQQTKD NVDRIKKIMA EWTKQPLFER KELKKESLLA LDDRQDRLKK RYAEITTAGE
KIHSLMKENL DLFKAEASSD IWKAYVDYVD DMVIDGFFNC IHCTLTYLLE NTDPRHCAAP
LFEARLELQV PDMIFNPSLD YGIADGFYDL VEMLISDTYK MASLVNRLAE HNGQEHYQAD
LEGMDDLSDV RNDLMDRVQT IMTKAQEYRN SFDNYAYLYV DDRKEFMRQF LLYNHVLTTE
EIEAHAEDGV PECPPTLDQF KEQVDTYEKI YSEADEIEPE QVFDAWFRVD SKPFKAALLN
IIKKWSFMFK QHLIDHVTNS LSELQEFIKV GNSGLTKTVE DGDYNGLVDC MGHLMAVKER
QAATDEMFEP IKQTIELLKT YDQEMSEEVH TQLQELPEQW NNTKKIAITI KQQVAPLQAN
EVAIIRRKCT SFDVRQHEFR ERFRKEAPFI FLFDGPYQCL DKCHSEIYEM EEHMAKLQES
AGLFEVNMPD YKQLKACRRE VRLLKGLWDL IMVVRTSIED WKTTPWLEIN VEQMEMDCKK
FAKDIRSLDK EMRAWDAYNG LDATVKNMLT SLRAVSELQN PAIRERHWQQ LMAATKVKFT
MDKETTLSDL LALNLHNFED EVRNIVDKAV KEMGMEKVLK ELNTTWSSMD FDYEPHSRTG
ISLLKSNEEL IETLEDNQVQ LQNLMTSKHI AHFLEEVSGW QKKLSTTDSV ITIWFEVQRT
WSHLESIFIG SEDIRNQLPE DSKRFDGIDT DFKELAAEME KTPNVVEATN KARLFDRLEA
IQGSLVVCEK ALAEYLETKR LAFPRFYFVS SADLLDILSQ GNNPTQVQRH LSKLFDNMAK
LKFKQDDEGN DTKLALGMYS KEGEYVDFDK ECECTGQVEV WLNRVMDTMR STVRSQFADA
VVSYEEKPRE QWLYDYPAQV ALATTQVWWT TEVNISFARL EEGHENSMKD YNKKQILQLN
TLIGLLIGKL TKGDRQKIMT ICTIDVHARD VVAMMVLKKV DSAQAFQWLS QLRHRWADDD
KHCYANICDA QFKYSYEYLG NTPRLVITPL TDRCYITLTQ SLHLVMSGAP AGPAGTGKTE
TTKDLGRALG IMVYVFNCSE QMDYKSCGNI YKGLAQTGAW GCFDEFNRIS VEVLSVVAVQ
VKCVQDAIRD KKERFNFMGE EISLIPSVGI FITMNPGYAG RTELPENLKA LFRPCAMVVP
DFELICEIML VAEGFLEARL LARKFITLYT LCKELLSKQD HYDWGLRAIK SVLVVAGSLK
RGDPQRPEDQ VLMRALRDFN VPKIVSDDTP VFMGLIGDLF PALDVPRRRD LDFEKVVKQS
TLDLKLQAED SFVLKVVQLE ELLAVRHSVF VIGNAGTGKS QVLKVLNKTY SNMKRKPVFI
DLNPKAVTND ELFGIINPAT REWKDGLFSV IMRDMSNITH DGPKWIVLDG DIDPMWIESL
NTVMDDNKVL TLASNERIPL TPSMRLLFEI SHLKTATPAT VSRAGILYIN PSDLGWNPIV
TSWIDTREVQ SERANLTILF DKYLPTLLDT LRIRFKKIIP IPEQSMVQML CYLLECLLTP
ENTPADCPKE LYELYFVFAS IWAFGGSMFQ DQLVDYRVEF SKWWITEFKT IKFPNQGTVF
DYYIDQESKK FLPWSEKVPT FELDPEIPMQ AVLVHTNETT RVRFFMDLLM ERGRPVMLVG
NAGLGKSVLV GDKLSNLGED SMVANVPFNY YTTSEMLQRV LEKPLEKKAG RNYGPPGTKK
LVYFIDDMNM PEVDTYGTVQ PHTLIRQHMD YKHWYDRAKL TLKEIHKCQY VSCMNPTSGS
FTINSRLQRH FCVFALSFPG QDALSTIYNS ILSQHLANIA VSNALQKLSP TVVSATLDLH
KKVAQSFLPT AIKFHYVFNL RDLSNVFQGL LYSGSDLLKS PIDFARLWMH ECQRVYGDKM
INDQDIEAFE KLVFEYAKKF FEDVDEEALK AKPNIHCHFA TGIGDPKYMP CATWPELNKI
LVEALDTYNE INAVMNLVLF EDAMQHVCRI NRILESPRGN ALLVGVGGSG KQSLARLASY
ISSLEVFQIT LRKGYGIPDL KLDLATVCMK AGLKNIGTVF LMTDAQVSDE KFLVLINDLL
ASGEIPDLFA DDEVENIIGG VRNEVKGMGL QDTRENCWKF FIDRLRRQLK TVLCFSPVGT
TLRVRSRKFP AVVNCTSIDW FHEWPQEALV SVSKRFLDEV ELLKGDIKNS IAEFMAYVHV
SVNESSKQYL TNERRYNYTT PKSFLEQIKL YESLLAMKSK ELTAKMERLE NGLTKLQSTA
QQVDDLKAKL ASQEVELAQK NEDADKLIQV VGVETEKVSK EKATVDDEEK KVAIINEEVS
KKAKDCSEDL AKAEPALLAA QEALNTLNKN NLTELKSFGS PPSAVLKVAA AVMVLLAPNG
KIPKDRSWKA AKVVMNKVDA FLDSLINYDE ENIHENCQKA IKEYLNDPEF EPEYIKGKSL
AAGGLCSWVV NIVKFYNVYC DVEPKRIALQ KANDELKAAQ DKLALIKAKI AELDANLAEL
TAQFEKATSD KLKCQQEAEA TSRTITLANR LVGGLASENV RWGEAVANFK IQEKTLPGDV
LLITAFVSYI GCFTKNYRVD LQDRMWLPFL KSQKDPIPIT EGLDVLSMLT DDADIAVWNN
EGLPSDRMST ENATILSNCQ RWPLMIDPQL QGIKWIKQKY GDELRVIRIG QRGYLDTIEN
AISSGDTVLI ENMEESIDPV LDPVLGRNTI KKGRYIKIGD KEVEYNPEFR LILQTKLANP
HYKPEMQAQT TLINFTVTRD GLEDQLLANV VAQERPDLEK LKSDLTKQQN DFKIILKELE
DNLLSRLSSA EGNFLGDTAL VENLETTKRT AAEISVKVEE AKVTEVKINE ARELYRPAAA
RASLLYFILN DLNKINPIYQ FSLKAFNTVF SLSIARAEPC EDVKERVVNL IDCITYSVFI
YTTRGLFEAD KLIFTTQVAF QVLLMKKEIA QNELDFLLRF PIQVGLTSPV DFLTNSAWGA
IKSLSAMEDF RNLDRDIEGS AKRWKKFVES ECPEKEKFPQ EWKNKSALQK LCMMRALRAD
RMSYAVRNFI EEKLGSKYVE GRQVEFAKSY EETDPATPVF FILSPGVDPL KDVEALGKKL
GFTFDNNNFH NVSLGQGQEI VAEQCMDLAA KEGHWVILQN IHLVAKWLST LEKKLEQYSI
GSHESYRVYM SAEPAGSPES HIIPQGILES SIKITNEPPT GMFANLHKAL YNFNQDTLEM
CAREAEFKVI LFALCYFHAV VCERQKFGPQ GWNRSYPFNT GDLTISVNVL YNYLEANSKV
PWQDLRYLFG EIMYGGHITD DWDRRLCRTY LEEYMAPEML DGDLYLAPGF PVPPNSDYKG
YHQYIDEILP PESPYLYGLH PNAEIGFLTT ESDNLFKVVL ELQPRDAGGG GGGGSSREEK
IKSLLDEIVE KLPEEFNMME IMGKVEDRTP YVVVAFQECE RMNTLTSEIR RSLKELDLGL
KGELTITPDM EDLSNALFLD QIPASWVKRA YPSLFGLSAW YADLLQRIKE LEQWTADFAL
PNVVWLGGFF NPQSFLTAIM QSMARKNEWP LDKMCLQCDV TKKNKEDFSS APREGSYVHG
LFMEGARWDT QTNMIADARL KELAPNMPVI FIKAIPVDKQ DTRNIYECPV YKTKQRGPTF
VWTFNLKSKE KAAKWTLAGV ALLLQV