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DYHC_SCHPO
ID   DYHC_SCHPO              Reviewed;        4196 AA.
AC   O13290; Q9P6L0; Q9UTP8;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 166.
DE   RecName: Full=Dynein heavy chain, cytoplasmic;
DE   AltName: Full=Dynein heavy chain, cytosolic;
DE            Short=DYHC;
GN   Name=dhc1; ORFNames=SPAC1093.06c, SPAC30C2.01c;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND SUBCELLULAR LOCATION.
RC   STRAIN=CRL152;
RX   PubMed=10366596; DOI=10.1083/jcb.145.6.1233;
RA   Yamamoto A., West R.R., McIntosh J.R., Hiraoka Y.;
RT   "A cytoplasmic dynein heavy chain is required for oscillatory nuclear
RT   movement of meiotic prophase and efficient meiotic recombination in fission
RT   yeast.";
RL   J. Cell Biol. 145:1233-1249(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
CC   -!- FUNCTION: Cytoplasmic dynein acts as a motor for the intracellular
CC       retrograde motility of vesicles and organelles along microtubules.
CC       Dynein has ATPase activity; the force-producing power stroke is thought
CC       to occur on release of ADP. Required for nuclear movement during
CC       meiotic prophase. {ECO:0000269|PubMed:10366596}.
CC   -!- SUBUNIT: Consists of at least two heavy chains and a number of
CC       intermediate and light chains.
CC   -!- INTERACTION:
CC       O13290; O42667: ssm4; NbExp=2; IntAct=EBI-1112490, EBI-1556587;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton
CC       {ECO:0000269|PubMed:10366596}. Cytoplasm, cytoskeleton, microtubule
CC       organizing center, spindle pole body {ECO:0000269|PubMed:10366596}.
CC       Note=Localized at the astral microtubules and the spindle pole body
CC       (SPB) during nuclear movements associated with meiotic prophase.
CC   -!- DOMAIN: Dynein heavy chains probably consist of an N-terminal stem
CC       (which binds cargo and interacts with other dynein components), and the
CC       head or motor domain. The motor contains six tandemly-linked AAA
CC       domains in the head, which form a ring. A stalk-like structure (formed
CC       by two of the coiled coil domains) protrudes between AAA 4 and AAA 5
CC       and terminates in a microtubule-binding site. A seventh domain may also
CC       contribute to this ring; it is not clear whether the N-terminus or the
CC       C-terminus forms this extra domain. There are four well-conserved and
CC       two non-conserved ATPase sites, one per AAA domain. Probably only one
CC       of these (within AAA 1) actually hydrolyzes ATP, the others may serve a
CC       regulatory function.
CC   -!- SIMILARITY: Belongs to the dynein heavy chain family. {ECO:0000305}.
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DR   EMBL; AB006784; BAA22056.1; -; Genomic_DNA.
DR   EMBL; CU329670; CAB60251.1; -; Genomic_DNA.
DR   PIR; T43274; T43274.
DR   PIR; T50069; T50069.
DR   RefSeq; XP_001713108.1; XM_001713056.2.
DR   SMR; O13290; -.
DR   BioGRID; 280476; 16.
DR   IntAct; O13290; 3.
DR   STRING; 4896.SPAC1093.06c.1; -.
DR   iPTMnet; O13290; -.
DR   PaxDb; O13290; -.
DR   PRIDE; O13290; -.
DR   EnsemblFungi; SPAC1093.06c.1; SPAC1093.06c.1:pep; SPAC1093.06c.
DR   PomBase; SPAC1093.06c; dhc1.
DR   VEuPathDB; FungiDB:SPAC1093.06c; -.
DR   eggNOG; KOG3595; Eukaryota.
DR   HOGENOM; CLU_000038_7_0_1; -.
DR   InParanoid; O13290; -.
DR   OMA; FIMDEAN; -.
DR   PhylomeDB; O13290; -.
DR   Reactome; R-SPO-6798695; Neutrophil degranulation.
DR   Reactome; R-SPO-9646399; Aggrephagy.
DR   PRO; PR:O13290; -.
DR   Proteomes; UP000002485; Chromosome I.
DR   GO; GO:0005938; C:cell cortex; IDA:PomBase.
DR   GO; GO:0030981; C:cortical microtubule cytoskeleton; IDA:PomBase.
DR   GO; GO:0005868; C:cytoplasmic dynein complex; ISO:PomBase.
DR   GO; GO:0005881; C:cytoplasmic microtubule; IBA:GO_Central.
DR   GO; GO:0030286; C:dynein complex; IBA:GO_Central.
DR   GO; GO:1990574; C:meiotic spindle astral microtubule; IDA:PomBase.
DR   GO; GO:0035974; C:meiotic spindle pole body; IDA:PomBase.
DR   GO; GO:0005524; F:ATP binding; ISM:PomBase.
DR   GO; GO:0045505; F:dynein intermediate chain binding; IBA:GO_Central.
DR   GO; GO:0051959; F:dynein light intermediate chain binding; IBA:GO_Central.
DR   GO; GO:0008569; F:minus-end-directed microtubule motor activity; IBA:GO_Central.
DR   GO; GO:0051315; P:attachment of mitotic spindle microtubules to kinetochore; IMP:PomBase.
DR   GO; GO:0031122; P:cytoplasmic microtubule organization; IBA:GO_Central.
DR   GO; GO:0030989; P:dynein-driven meiotic oscillatory nuclear movement; IDA:PomBase.
DR   GO; GO:0051293; P:establishment of spindle localization; IBA:GO_Central.
DR   GO; GO:0007129; P:homologous chromosome pairing at meiosis; IMP:PomBase.
DR   GO; GO:0000742; P:karyogamy involved in conjugation with cellular fusion; IMP:PomBase.
DR   GO; GO:0007018; P:microtubule-based movement; IBA:GO_Central.
DR   GO; GO:0072382; P:minus-end-directed vesicle transport along microtubule; IBA:GO_Central.
DR   GO; GO:0000278; P:mitotic cell cycle; IBA:GO_Central.
DR   GO; GO:1990758; P:mitotic sister chromatid biorientation; IMP:PomBase.
DR   GO; GO:0007097; P:nuclear migration; IBA:GO_Central.
DR   Gene3D; 1.10.8.710; -; 1.
DR   Gene3D; 1.10.8.720; -; 1.
DR   Gene3D; 1.20.140.100; -; 1.
DR   Gene3D; 3.20.180.20; -; 1.
DR   Gene3D; 3.40.50.300; -; 5.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR035699; AAA_6.
DR   InterPro; IPR035706; AAA_9.
DR   InterPro; IPR041658; AAA_lid_11.
DR   InterPro; IPR042219; AAA_lid_11_sf.
DR   InterPro; IPR026983; DHC_fam.
DR   InterPro; IPR042222; Dynein_2_N.
DR   InterPro; IPR043157; Dynein_AAA1S.
DR   InterPro; IPR041466; Dynein_AAA5_ext.
DR   InterPro; IPR024743; Dynein_HC_stalk.
DR   InterPro; IPR024317; Dynein_heavy_chain_D4_dom.
DR   InterPro; IPR004273; Dynein_heavy_D6_P-loop.
DR   InterPro; IPR013602; Dynein_heavy_linker.
DR   InterPro; IPR013594; Dynein_heavy_tail.
DR   InterPro; IPR042228; Dynein_linker_3.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR10676; PTHR10676; 1.
DR   Pfam; PF12774; AAA_6; 1.
DR   Pfam; PF12780; AAA_8; 1.
DR   Pfam; PF12781; AAA_9; 1.
DR   Pfam; PF18198; AAA_lid_11; 1.
DR   Pfam; PF08385; DHC_N1; 1.
DR   Pfam; PF08393; DHC_N2; 1.
DR   Pfam; PF17852; Dynein_AAA_lid; 1.
DR   Pfam; PF03028; Dynein_heavy; 1.
DR   Pfam; PF12777; MT; 1.
DR   SMART; SM00382; AAA; 3.
DR   SUPFAM; SSF52540; SSF52540; 4.
PE   1: Evidence at protein level;
KW   ATP-binding; Coiled coil; Cytoplasm; Cytoskeleton; Dynein; Microtubule;
KW   Motor protein; Nucleotide-binding; Reference proteome; Repeat.
FT   CHAIN           1..4196
FT                   /note="Dynein heavy chain, cytoplasmic"
FT                   /id="PRO_0000114642"
FT   REGION          1..1851
FT                   /note="Stem"
FT                   /evidence="ECO:0000250"
FT   REGION          1852..2069
FT                   /note="AAA 1"
FT                   /evidence="ECO:0000250"
FT   REGION          2135..2379
FT                   /note="AAA 2"
FT                   /evidence="ECO:0000250"
FT   REGION          2484..2728
FT                   /note="AAA 3"
FT                   /evidence="ECO:0000250"
FT   REGION          2822..3082
FT                   /note="AAA 4"
FT                   /evidence="ECO:0000250"
FT   REGION          3315..3403
FT                   /note="Stalk"
FT                   /evidence="ECO:0000250"
FT   REGION          3471..3696
FT                   /note="AAA 5"
FT                   /evidence="ECO:0000250"
FT   REGION          3874..4085
FT                   /note="AAA 6"
FT                   /evidence="ECO:0000250"
FT   COILED          1217..1252
FT                   /evidence="ECO:0000255"
FT   COILED          3315..3403
FT                   /evidence="ECO:0000255"
FT   COILED          3649..3666
FT                   /evidence="ECO:0000255"
FT   BINDING         1890..1897
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
FT   BINDING         2169..2176
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
FT   BINDING         2174..2181
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
FT   BINDING         2520..2527
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   4196 AA;  484318 MW;  8F10AE370184FC0C CRC64;
     MDKNDNSQCQ LSTVSDEILI FLKKLLSLSV SDNLDDICET IALQSTFVDT FRSFINDEYY
     TVIYLYGSPC LTSSPTSPDS CTFGNMTFQW TSLLQDVFSG TSAFAIFKRF PLTDTPLTLQ
     NMLYINQLPI LKGGHKSNEI PERPINAIFL YTKYVMSCYF TAYLAMESVD DRSTIDLNSP
     SKGKELELTC QKFADFERSF TFFCREYQNS DTILQHHPLI LSTIKHAEEN NLDLSVRLLP
     SKVLSDSEFY KSLSNLVNVW LKTTRSLIKL FHDQISKTAL EEFNFWQFYY RSLSRLNDQL
     HSRPVLFVLD ILAFGKRFHT IASFNSETNI QCFVDKVGKI DALFKEISLD IFLSSSTLES
     LQLSAALLYS TFSKKWRNTG YPETRVLDFI NFITEDLLKL ISRLLPALGA LSLSNVDFSH
     RTAVSSDILS LCYIRLKDFL RISGSLKEEQ SYYGLKNSIK QIKAFENKLK YIQSFHEKHQ
     QLIGALSEVY GLTHLTELEI LEHLNKKEHV FNILTVFKDL QSLNVLDISL KGVNAWNSLE
     TSYYNCMTVL EDEVIAQLKS LLQYSKTSSQ MFTTLMRFQP LFFRTRVRTS ISDCLHLLVN
     RIKQELDLLK TRFTEDVSDT ELIAMNELRN LPMASSAIIW ATQLKNRLHE YTKNINIIFG
     EDWNNFPDGF ELKVECITLQ KRLDTNLIFT NWINDVSSRN LNFDFDSKIF YLTQSESAES
     PLRLSVSIDF DPCSFCKEIR TLAHLGYNIP SQLMELASCL QRIQLIAMCL IDSVQSFNDV
     SFEISKTEEE RFLLQEYELA VRQHIVTGLF ISWNDFIVGN LSTPPKCAIG KRNFLKNIHP
     NVENYAYQFS SLTSLLMNKR NAISHTYMQI QEQLFQLDIC EYSGDIFLTI QRKLQDLIDL
     LYVNGYSNLP PFVRALNLRF QDLLISRCRK FLSFFKTTIL TSGNENNDLK SKFSSDMYEK
     LRGFLKPTNL TIQRNIIEFD PPVYRKKEDS IYLLDMCLQS VVNIPLLSIK TTAQRNCTLI
     GFFPVINRLE SEILGIFESL LFHFDSILGY QNYWKKVEPF LNLNSLNLLL KSELFQLDQC
     YSLSLYLIHL KSEVDEIGKV TDFKIFSVNN TEFKSQVYLY LREWINALFD RFTFLLGKES
     EHLLNELDDT HSSLSTVDFT VNNTESLINS LKIFKKAGCY KLNVEHKIIT YQNYEMTFND
     CDAFSEFNFS LLKDITSKWK DLLESFECRR LKLENNKDEI LRNFSEFAKR VNTETLSLIS
     EWCASSLSLI KANYDEFSST VDDFLFRFSK ATEQCLMVKY IKKDLEIEIE ESCDFSIQTE
     EIHLYKKFKD VISSNLEFIV EIKNTRWKLF DTATLSVQTT HQINALESVH TSFQHFKLFT
     NTKQSLNQLK DCTLLLQKLK SCPLKPVHWI SLFEITKSTE QLDFEKLLVS DILGIDLQAH
     ESFITTLLNS AVVEANLENQ FNEVHSFWKN SYFSFKSFKG RNYIVVGCQE LIDAVEKNMD
     SLNLIKTSRH FKDGDMNITD LQSKMKIIVK FLNIWKEIQQ IWTHLSAIFY ESTYIQQLLP
     ELAASFFNSS KTYMHLVTLL KERSYLYKVS NIPSLLESAA KLSTTLEDSK KSLLKYFELQ
     RHKISRLYFL GDDDLMELIS NPCDPFVINK QIIKLYPGIR SLIVDTENTN INGCTTNEGN
     ELLFDNPICL LDNTQPLHWI SSLEPFLKAT LFQLFSTSFQ QIRDFYYNKS RNVFCKEWFL
     RYPSQITLLS LRCTLCHEIE TGIADCCLDA VFNFINDGIS SLVLLADENE LSIKKKVTLM
     FNELLHFKET VGLLCKNSFN NYFWSREVKA FYREDHDDEA VVIKMFSLEF IYAFEYSELD
     DPIVYTDLTR NCFSVLLHSI ASNLGGSPIG PAGTGKTETV KAVSAYLGKN VFVFNCDNAF
     NYKTIQRILS GLAQIGTYIC FDEFNRLDSG TLSAISYDIQ RIQSLVSHSD GLCQSPILLD
     APTIFVTMNP GYLGRFKLPS NLKKLFRPIW MGSPDNKKIC EILFLSFGFK ESSLLSQVLD
     SFFLCCSGSL SNCLHYDFGL RAMKVVIKAA KRIKGFLKKK NTICQELEIL WYAIREVLYP
     SLIYQDIPLF FKAEESYFNF PAVKANAFID PDNFEVNIEQ TLSKNFFGNN QYLKLKIMQL
     YQMSEAYNGI ILLGKTGSGK SQIFRILQSA LLNIGIDCIV YVISPKALTK ESLFGSMNMD
     TREWTDGVFT KLLRKTRDSC YYKRYMFVFD DELSPEWVEA MNSLLDDNKT LTLSNGERIA
     LQPYVKIFFE ADSVASLTRA TISRCGLICI SNIDDNILSS TDKMLSFTSG ATNYPLGSSN
     DEFSTVFSKV LTDEVMMNLI SSCYKFSVDL QHIMNFTKQR FFTTFYSLLD QTKLFTRSSN
     ITESLSFKEL CNYLKKKICY ILAWCCTGDT DAKSRERFTH WLMQNASVDL PEIKDFEHVS
     ILDFDVSLET QSWYPIAGKT LKSSALKYAG NTVIPTLDTV RYAEFLNFSL TKNRCVIFCG
     PPGSGKSMLM LGTLRSRQDV EVIALNFSIS TSSKSVVSFL EQSTVYYRST GMTIMCPKNH
     EKVLVLFCDE INLPRSRNCL AEDVICFLRH MLEHQGFWHP LHKEWVTIKN IFVCGACNPS
     TDIGRNDFPE RFLRRTVLIF VDYPESYSLV TIYNALLEKS ALINQYKTII LNIVKASVKF
     YQVLRENFKS STQGYVYTPR DLTRWLISFK NYAESYAETN NLSLIKVWYH EACRVLLDRL
     VSQKECSWGM TELQKVIVTD FGEFEVSVIF EKQIIFTDIL KNGLEFLDFA SLRPKLESLY
     KKFYSSHPNN TLVFVDETIT HILRFHRILN NSGMHALLQG SVGLGQKAVV EFVCWLNSFS
     LFELQKNQTY SIEDFEDNLK SILILAGTTN CKACLAINES IAGVPGFLDL LNNLLTNSEV
     SNFFDQNDWA EIKKNLNKLN EFQPLKFDSE ESVTEIFMNN VFQNLCVVFY VYTSADVDFQ
     TNSLSPALLN RCTIDYYHSW DYHSMLQIAN EVLQETISLN ALDHDNPNLK NIKGSSIYDA
     VAQAVVNTHT SIVWEFKHLG KTSYFSCLHF IRFLNTFCLI FGRDANKLSK EKSRIENGFK
     KIKETSQGID KFKEALSDQQ NVLFSKTKTA NDRLQCIIQT KQAVEAKKVY SLQAEASLQK
     KSFLLNEKKN SVMKEVSYAK PAVIEARKSV SDIKKAHLIE LRSLSRPPMA IRITMEVVCK
     LLGFSATDWK NVQQLLKRDD FIPKILNYNL EKELSINLRR KIEQDYFSNP IFTFDSVNRA
     SKACGPLLLW IKSICNYSKV LEKLEPLNSE VDRLKLEQKN AEECIQETIA ACKDLDEKLL
     QLQEEYASMI SEIHSMELQM DEVKCKMQRS IEVITDLSIE RNEWSGFLNL YPKRMWNLVG
     ESLMEASFVV YAGNLDPSMR IFLRNKCEPI ISSFGFPISK SAVRTNIERC VQTSIESKYY
     KNLTDYSLEN IYIIQENKSP LLIIDPSSQI LDILPSLYKG KASDLISFSN KSFQNQIKLA
     LLSGSAIIIK DAELWDVSIE PLLKPEFFTG SGEVQTTFAK DTITITLPLN IIFFSEVQSN
     ELENKASKFM NVVNFTLSIS LLETQMLKSV ISVQEPGVFK QKDNCFTLKL SIERQIRSLQ
     EQLLKTLCSS NENIVGTDEI VVLLKNLKEK HETIRLAYSE SQSINRKVDE LIRRYKLSIK
     SFLSVVVVFQ HFISLKKSYS FSFNFIWSTF HQMLNVVLEN RNQDFKSLIM DALRDLIRRC
     FLYIFPEDRV LFLFLLMFFF FPKETESLRK LLIVNGKTLE LEQSYLNFFE TCSDSNERGG
     LESLFFKTHA SNIQNFCTEV LANTHCEEDC LKLLYDLWSS AFKVEFSNIK YDFLKIINDE
     SESRMPTIVY LMENCEIDSL LQNAKIPQNI KKLTVSLGSA ENESLADSYL KLASTEPLWL
     FINNIHLSTP WAEKLPSKMS NHLHKNSRIV CLSEIHNQLP HQLLCISRSI VFNKQTSFKN
     NLLNLLELLP TMTHTLPHNR FRLFFFLSWL HATLAEIYCF TCSSWKEPCY FDDSDFYFGT
     KILCNILYRN VHLEEFSWGT FKDLLLNVVY GPKVSASSDF IALDKILKRL IAQFKTQISS
     NILLTDNFKF ILPYEITFSS AKEVIGQLPD EIPPGWLDIP ENSKRKRTDI YFSMCI
 
 
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