ID   DYHC_TRIGR              Reviewed;        4466 AA.
AC   P23098;
DT   01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1991, sequence version 1.
DT   03-AUG-2022, entry version 104.
DE   RecName: Full=Dynein beta chain, ciliary;
OS   Tripneustes gratilla (Hawaian sea urchin) (Echinus gratilla).
OC   Eukaryota; Metazoa; Echinodermata; Eleutherozoa; Echinozoa; Echinoidea;
OC   Euechinoidea; Echinacea; Temnopleuroida; Toxopneustidae; Tripneustes.
OX   NCBI_TaxID=7673;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 162-172; 1193-1204;
RP   3240-3259 AND 3325-3339.
RC   TISSUE=Blastula, and Sperm;
RX   PubMed=1830927; DOI=10.1038/352640a0;
RA   Gibbons I.R., Gibbons B.H., Mocz G., Asai D.J.;
RT   "Multiple nucleotide-binding sites in the sequence of dynein beta heavy
RT   chain.";
RL   Nature 352:640-643(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=1833761; DOI=10.1073/pnas.88.19.8563;
RA   Gibbons I.R., Asai D.J., Ching N.S., Dolecki G.J., Mocz G.,
RA   Phillipson C.A., Ren H., Tang W.Y., Gibbons B.H.;
RT   "A PCR procedure to determine the sequence of large polypeptides by rapid
RT   walking through a cDNA library.";
RL   Proc. Natl. Acad. Sci. U.S.A. 88:8563-8567(1991).
RN   [3]
RP   3D-STRUCTURE MODELING.
RX   PubMed=11250194; DOI=10.1016/s0969-2126(00)00557-8;
RA   Mocz G., Gibbons I.R.;
RT   "Model for the motor component of dynein heavy chain based on homology to
RT   the AAA family of oligomeric ATPases.";
RL   Structure 9:93-103(2001).
CC   -!- FUNCTION: Force generating protein of eukaryotic cilia and flagella.
CC       Produces force towards the minus ends of microtubules. Dynein has
CC       ATPase activity; the force-producing power stroke is thought to occur
CC       on release of ADP.
CC   -!- SUBUNIT: Consists of at least two heavy chains (alpha and beta), three
CC       intermediate chains and several light chains.
CC   -!- SUBCELLULAR LOCATION: Cell projection, cilium, flagellum. Cytoplasm,
CC       cytoskeleton, flagellum axoneme.
CC   -!- DOMAIN: Dynein heavy chains probably consist of an N-terminal stem
CC       (which binds cargo and interacts with other dynein components), and the
CC       head or motor domain. The motor contains six tandemly-linked AAA
CC       domains in the head, which form a ring. A stalk-like structure (formed
CC       by two of the coiled coil domains) protrudes between AAA 4 and AAA 5
CC       and terminates in a microtubule-binding site. A seventh domain may also
CC       contribute to this ring; it is not clear whether the N-terminus or the
CC       C-terminus forms this extra domain. There are four well-conserved and
CC       two non-conserved ATPase sites, one per AAA domain. Probably only one
CC       of these (within AAA 1) actually hydrolyzes ATP, the others may serve a
CC       regulatory function.
CC   -!- SIMILARITY: Belongs to the dynein heavy chain family. {ECO:0000305}.
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DR   EMBL; X59603; CAA42170.1; -; mRNA.
DR   PIR; S17653; S17653.
DR   SMR; P23098; -.
DR   PRIDE; P23098; -.
DR   GO; GO:0097729; C:9+2 motile cilium; IEA:UniProt.
DR   GO; GO:0005858; C:axonemal dynein complex; IEA:InterPro.
DR   GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0045505; F:dynein intermediate chain binding; IEA:InterPro.
DR   GO; GO:0051959; F:dynein light intermediate chain binding; IEA:InterPro.
DR   GO; GO:0008569; F:minus-end-directed microtubule motor activity; IEA:InterPro.
DR   GO; GO:0030030; P:cell projection organization; IEA:UniProtKB-KW.
DR   GO; GO:0003341; P:cilium movement; IEA:InterPro.
DR   Gene3D; 1.10.8.710; -; 1.
DR   Gene3D; 1.10.8.720; -; 1.
DR   Gene3D; 1.20.140.100; -; 1.
DR   Gene3D; 3.10.490.20; -; 1.
DR   Gene3D; 3.20.180.20; -; 1.
DR   Gene3D; 3.40.50.300; -; 5.
DR   InterPro; IPR035699; AAA_6.
DR   InterPro; IPR035706; AAA_9.
DR   InterPro; IPR041658; AAA_lid_11.
DR   InterPro; IPR042219; AAA_lid_11_sf.
DR   InterPro; IPR026983; DHC_fam.
DR   InterPro; IPR041589; DNAH3_AAA_lid_1.
DR   InterPro; IPR030443; DNAH9-like.
DR   InterPro; IPR042222; Dynein_2_N.
DR   InterPro; IPR043157; Dynein_AAA1S.
DR   InterPro; IPR041466; Dynein_AAA5_ext.
DR   InterPro; IPR041228; Dynein_C.
DR   InterPro; IPR043160; Dynein_C_barrel.
DR   InterPro; IPR024743; Dynein_HC_stalk.
DR   InterPro; IPR024317; Dynein_heavy_chain_D4_dom.
DR   InterPro; IPR004273; Dynein_heavy_D6_P-loop.
DR   InterPro; IPR013602; Dynein_heavy_linker.
DR   InterPro; IPR013594; Dynein_heavy_tail.
DR   InterPro; IPR042228; Dynein_linker_3.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR10676; PTHR10676; 1.
DR   PANTHER; PTHR10676:SF257; PTHR10676:SF257; 1.
DR   Pfam; PF12774; AAA_6; 1.
DR   Pfam; PF12780; AAA_8; 1.
DR   Pfam; PF12781; AAA_9; 1.
DR   Pfam; PF17857; AAA_lid_1; 1.
DR   Pfam; PF18198; AAA_lid_11; 1.
DR   Pfam; PF08385; DHC_N1; 1.
DR   Pfam; PF08393; DHC_N2; 1.
DR   Pfam; PF17852; Dynein_AAA_lid; 1.
DR   Pfam; PF18199; Dynein_C; 1.
DR   Pfam; PF03028; Dynein_heavy; 1.
DR   Pfam; PF12777; MT; 1.
DR   SUPFAM; SSF52540; SSF52540; 4.
PE   1: Evidence at protein level;
KW   ATP-binding; Cell projection; Cilium; Cilium biogenesis/degradation;
KW   Coiled coil; Cytoplasm; Cytoskeleton; Direct protein sequencing; Dynein;
KW   Flagellum; Microtubule; Motor protein; Nucleotide-binding; Repeat.
FT   CHAIN           1..4466
FT                   /note="Dynein beta chain, ciliary"
FT                   /id="PRO_0000114645"
FT   REGION          1..1813
FT                   /note="Stem"
FT                   /evidence="ECO:0000305"
FT   REGION          1814..2035
FT                   /note="AAA 1"
FT                   /evidence="ECO:0000305"
FT   REGION          2095..2316
FT                   /note="AAA 2"
FT                   /evidence="ECO:0000305"
FT   REGION          2422..2669
FT                   /note="AAA 3"
FT                   /evidence="ECO:0000305"
FT   REGION          2767..3016
FT                   /note="AAA 4"
FT                   /evidence="ECO:0000305"
FT   REGION          3033..3325
FT                   /note="Stalk"
FT                   /evidence="ECO:0000305"
FT   REGION          3409..3636
FT                   /note="AAA 5"
FT                   /evidence="ECO:0000305"
FT   REGION          3846..4072
FT                   /note="AAA 6"
FT                   /evidence="ECO:0000305"
FT   COILED          733..805
FT                   /evidence="ECO:0000255"
FT   COILED          1036..1056
FT                   /evidence="ECO:0000255"
FT   COILED          1306..1337
FT                   /evidence="ECO:0000255"
FT   COILED          1443..1468
FT                   /evidence="ECO:0000255"
FT   COILED          3033..3092
FT                   /evidence="ECO:0000255"
FT   COILED          3263..3325
FT                   /evidence="ECO:0000255"
FT   COILED          3573..3642
FT                   /evidence="ECO:0000255"
FT   BINDING         154..161
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
FT   BINDING         1852..1859
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
FT   BINDING         2133..2140
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
FT   BINDING         2460..2467
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
FT   BINDING         2805..2812
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
FT   VARIANT         611..615
FT                   /note="Missing"
FT   VARIANT         3357
FT                   /note="P -> LLTGNFFCCFMTA"
SQ   SEQUENCE   4466 AA;  511782 MW;  2A695BF8F336911E CRC64;
     MADVVDPRLE FISEYILKSY KLKPDKWAKC INVEENKILM LEFLEKADNP QLVFTVNAAG
     LITPSYEFPS ALKNTKAIYF IKKGREPVGK DNIKTTLVYG DLSYTPLEQL SALVDEILVP
     LLANPRNHEQ WPVVVSQDVL RHVHNLKSSV YVVAGQVKGK TLLPLPVGSE KVETAAESEE
     KDDSYDRSLV HAIESVIIDW THQIRDVLKR DSAQPLLEGL NPGPMVEINF WKAKCENLDC
     IFQQLRDPKV RKMKELLERT QSSYLPSFNN IERDVEAALT EAQDINCYLK PLIYQVESLD
     ELEFPDMTPR LAPILHTVCL IWSNSDYYNT APRIIVLLQE ICNLLIDLCR TFLDPSEIFK
     LEPEESLEKV RGALAVLKAW RDLYDEHRAK LKDYFKDGRE VKGWEFASPL VFTRMDNFTS
     RIETIQSLFE TNVEFSKLEK TEMGSMKGRM LSQQVEKIHE EFQECAKVFT ERPYDGLDPQ
     CQEFLDDYEE FEKKVFDLDR RLGSILCQGF DDCCGLEAVF KMLDCYGPLL DRPVIRNDFE
     CKYPVVLMLY DLELDQAKEI YDEHMRVEEN DGNAPLNKNM PDVAGQLKWS AQLRDRISKP
     MGSLKHMEHP TGVRRILESE DAKVVFQKYE EMINLLNKYE QKVFENWTKG VDEVCKTNLD
     QSLITRDEST KLIKINFDPK LVAVLREVKY LQIRGEESIP ESAASIYEKH ETLRKYVANL
     DLTQAWYNKV RNTVLEVEFP LIEGQLADLD TRLKQAESEL NWTSDSVWEY IQETRDQVHD
     LEKRVQQTKD NVDRIKKIMA EWTKQPLFER KELKKESLLA LDDRQDRLKK RYAEISTAGE
     KIHSLIKENL GLFKADASSD IWKAYVDYVD DMVIDGFFNC IHCTLSYLLE NTDPRHCAAP
     LFEARLELQV PDMIFNPSLD YGVADGFYDL VEMLISDTYK MASLVSRLAE HNGQEHYQAD
     LEGMDDLSDV RNDLMDRVQT IMTKAQEYRN SFDNYAYLYV DDRKEFMRQF LLYNHVLTTE
     EIEAHAEDGV PECPPTLDQF KEQVDTYEKI YSEADEIEPE QVFDAWFRVD SKPFKAALLN
     IIKKWSFMFK QHLIDHVTNS LSELQEFIKV GNSGLTKTVE EGDYNGLVEC MGHLMAVKER
     QAATDEMFEP IKQTIELLKT YDQEMSEEVH TQLQELPEQW NNTKKIAITV KQQVAPLQAN
     EVAIIRRKCT SFDVRQHEFR ERFRKEAPFI FTFEGPYQCL DRCHSEIYEM EEHMANLQES
     AGLFEVNMPD YKQLKACRRE VRLLKALWDL IMIVRTSIED WKTTPWLEIN VEQMEMDCKK
     FAKDIRSLDK EMRAWDAYNG LDATVKNMLT SLRAVSELQN PAIRERHWQQ LMAATKVKFT
     MDKETTLSDL LALNLHNFED EVRNIVDKAV KEMGMEKVLK ELNTTWSSMD FEYEPHSRTG
     ISLLKSNEEL IETLEDNQVQ LQNLMTSKHI AHFLEEVSGW QKKLSTTDSV ITIWFEVQRT
     WSHLESIFIG SEDIRNQLPE DSKRFDGIDT DFKELASEME KTPNVVEATN RARLYDRLEA
     IQGSLVVCEK ALAEYLETKR LAFPRFYFVS SADLLDILSQ GNNPSQVQRH LSKLFDNMAK
     LKFKQDDEGN DTKLALGMYS KEGEYVDFDK ECECTGQVEV WLNRVMDAMR STVRSQFADA
     VVSYEEKPRE QWLYDYPAQV ALATTQVWWT TEVNISFARL EEGHENSMKD YNKKQIQQLN
     TLIGLLIGKL TKGDRQKIMT ICTIDVHARD VVAMMVLKKV DNAQAFQWLS QLRHRWADDD
     KHCYANICDA QFKYSYEYLG NTPRLVITPL TDRCYITLTQ SLHLVMSGAP AGPAGTGKTE
     TTKDLGRALG IMVYVFNCSE QMDYKSCGNI YKGLSQTGAW GCFDEFNRIS VEVLSVVAVQ
     VKCVQDAIRD KKERFNFMGE EISLIPSVGI FITMNPGYAG RTELPENLKA LFRPCAMVVP
     DFELICEIML VAEGFLDARL LARKFITLYT LCKELLSKQD HYDWGLRAIK SVLVVAGSLK
     RGDPQRPEDQ VLMRALRDFN VPKIVSDDTP VFMGLIGDLF PALDVPRRRD MDFEKVVKQS
     TLDLKLQAED SFVLKVVQLE ELLAVRHSVF VIGNAGTGKS QVLKVLNKTY SNMKRKPVLV
     DLNPKAVTND ELFGIINPAT REWKDGLFSV IMRDMSNITH DGPKWIVLDG DIDPMWIESL
     NTVMDDNKVL TLASNERIPL TPSMRLLFEI SHLKTATPAT VSRAGILYIN PSDLGWNPIV
     TSWIDTREVQ SERANLTILF DKYLPTLLDT LRVRFKKIIP IPEQSMVQML CYLLECLLTP
     ENTPADCPKE LYELYFVFAS IWAFGGSMFQ DQLVDYRVEF SKWWITEFKT IKFPNQGTVF
     DYFIDQESKK FLPWSEKVPV FELDPEIPMQ AVLVHTNETT RVRFFMDLLM ERGRPVMLVG
     NAGLGKSVLV GDKLSNLGED SMVANVPFNY YTTSEMLQRV LEKPLEKKAG RNYGPPGTKK
     LVYFIDDMNM PEVDTYGTVQ PHTLIRQHMD YKHWYDRQKL TLKEIHKCQY VSCMNPTAGS
     FTINSRLQRH FCVFALSFPG QDALSTIYNS ILSQHLANIS VSNALQKLSP TVVSATLDLH
     KKVAQSFLPT AIKFHYVFNL RDLSNVFQGL LYSGPDLLKA PIDFARLWMH ECQRVYGDKM
     INDQDIEAFE KLVLEYAKKF FEDVDEEALK AKPNIHCHFA TGIGDPKYMQ VPNWPELNKI
     LVEALDTYNE INAVMNLVLF EDAMQHVCRI NRILESPRGN ALLVGVGGSG KQSLARLASY
     ISSLEVFQIT LRKGYGIPDL KLDLATVCMK AGLKNIGTVF LMTDAQVSDE KFLVLINDLL
     ASGEIPDLFA DDEVENIIGG VRNEVKGMGL QDTRENCWKF FIDRLRRQLK TVLCFSPVGT
     TLRVRSRKFP AVVNCTSIDW FHEWPQEALV SVSMRFLDEV ELLKGDIKKS IAEFMAYVHV
     SVNESSKLYL TNERRYNYTT PKSFLEQIKL YESLLSMKSK ELTAKMERLE NGLTKLQSTA
     QQVDDLKAKL ASQEVELAQK NEDADKLIQV VGVETEKVSK EKAIADDEEK KVAIINEEVS
     KKAKDCSEDL AKAEPALLAA QEALNTLNKN NLTELKSFGS PPSAVLKVAA AVMVLLAPNG
     KIPKDRSWKA AKVVMNKVDA FLDSLINYDK ENIHENCLKS IKEYLNDPEF EPEYIKGKSL
     AAGGLCSWVV NIVKFYNVYC DVEPKRIALQ KANDELKAAQ DKLALIKAKI AELDANLAEL
     TAQFEKATSD KLKCQQEAEA TSRTITLANR LVGGLASENV RWGEAVANFK IQEKTLPGDV
     LLITAFVSYI GCFTKTYRVD LQERMWLPFL KSQKDPIPIT EGLDVLSMLT DDADIAVWNN
     EGLPSDRMST ENATILSNCQ RWPLMIDPQL QGIKWIKQKY GDDLRVIRIG QRGYLDTIEN
     AISSGDTVLI ENMEESIDPV LDPVLGRNTI KKGRYIKIGD KEVEYNPDFR LILQTKLANP
     HYKPEMQAQT TLINFTVTRD GLEDQLLANV VAQERPDLEK LKSDLTKQQN DFKIILKELE
     DNLLSRLSSA EGNFLGDTAL VENLETTKRT AAEISVKVEE AKVTEVKINE ARELYRPAAA
     RASLLYFILN DLNKINPIYQ FSLKAFNTVF SLPIARAEPC EDVKERVVNL IDCITYSVFI
     YTTRGLFEAD KLIFTTQVAF QVLLMKKEIA QNELDFLLRF PIQVGLTSPV DFLTNSAWGA
     IKSLSAMEDF RNLDRDIEGS AKRWKKFVES ECPEKEKFPQ EWKNKSALQK LCMMRALRAD
     RMSYAVRNFI EEKLGSKYVE GRQVEFAKSY EETDPATPVF FILSPGVDPL KDVEALGKKL
     GFTFDNNNFH NVSLGQGQEI VAEQCMDLAA KEGHWVILQN IHLVAKWLST LEKKLEQYSV
     GSHDSYRVYM SAEPAGSPEA HIIPQGILES SIKITNEPPT GMFANLHKAL YNFNQDTLEM
     CAREAEFKVI LFALCYFHAV VCERQKFGPQ GWNRSYPFNT GDLTISVNVL YNYLEANSKV
     PWQDLRYLFG EIMYGGHITD DWDRRLCRTY LEEYMAPEML DGELYLAPGF PVPPNSDYKG
     YHQYIDEILP PESPYLYGLH PNAEIGFLTT ESDNLFKIVL ELQPRDAGGG GGGGSSREEK
     IKSLLDEIVE KLPEEFNMME IMAKVEDRTP YVVVAFQECE RMNMLTSEIR RSLKELDLGL
     KGELTITPDM EDLSNALFLD QIPATWVKRA YPSLFGLTSW YADLLQRIKE LEQWTADFAL
     PNVVWLGGFF NPQSFLTAIM QSMARKNEWP LDKMCLQCDV TKKNKEDFSS APREGSYVHG
     LFMEGARWDT QTNMIADARL KELAPNMPVI FIKAIPVDKQ DTRNIYECPV YKTKQRGPTF
     VWTFNLKSKE KAAKWTLAGV ALLLQV