DYLT1_RAT
ID DYLT1_RAT Reviewed; 113 AA.
AC Q9Z336;
DT 27-SEP-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Dynein light chain Tctex-type 1;
DE AltName: Full=Activator of G-protein signaling 2;
DE Short=AGS2;
DE AltName: Full=T-complex testis-specific protein 1 homolog;
GN Name=Dynlt1; Synonyms=Tctel1, Tctex-1, Tctex1;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND INTERACTION WITH DOC2A AND DOC2B.
RC STRAIN=Sprague-Dawley; TISSUE=Brain;
RX PubMed=9804756; DOI=10.1074/jbc.273.46.30065;
RA Nagano F., Orita S., Sasaki T., Naito A., Sakaguchi G., Maeda M.,
RA Watanabe T., Kominami E., Uchiyama Y., Takai Y.;
RT "Interaction of Doc2 with tctex-1, a light chain of cytoplasmic dynein.
RT Implication in dynein-dependent vesicle transport.";
RL J. Biol. Chem. 273:30065-30068(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Horiuchi M., Betz H.;
RL Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP IDENTIFICATION IN THE CYTOPLASMIC DYNEIN 1 COMPLEX.
RX PubMed=11746667; DOI=10.1002/cm.1036;
RA Wilson M.J., Salata M.W., Susalka S.J., Pfister K.K.;
RT "Light chains of mammalian cytoplasmic dynein: identification and
RT characterization of a family of LC8 light chains.";
RL Cell Motil. Cytoskeleton 49:229-240(2001).
RN [4]
RP INTERACTION WITH SCN10A, AND IDENTIFICATION IN A COMPLEX WITH SCN10A.
RX PubMed=12591166; DOI=10.1016/s0169-328x(02)00661-7;
RA Malik-Hall M., Poon W.-Y.L., Baker M.D., Wood J.N., Okuse K.;
RT "Sensory neuron proteins interact with the intracellular domains of sodium
RT channel NaV1.8.";
RL Brain Res. Mol. Brain Res. 110:298-304(2003).
RN [5]
RP FUNCTION IN REGULATION OF NEURONAL MORPHOGENESIS, AND MUTAGENESIS OF
RP THR-94.
RX PubMed=15992542; DOI=10.1016/j.devcel.2005.04.003;
RA Chuang J.Z., Yeh T.Y., Bollati F., Conde C., Canavosio F., Caceres A.,
RA Sung C.H.;
RT "The dynein light chain Tctex-1 has a dynein-independent role in actin
RT remodeling during neurite outgrowth.";
RL Dev. Cell 9:75-86(2005).
RN [6]
RP INTERACTION WITH GNB1; GNB2; GNB3 AND GNB5, AND FUNCTION IN NEURONAL
RP MORPHOGENESIS.
RX PubMed=17491591; DOI=10.1038/sj.emboj.7601716;
RA Sachdev P., Menon S., Kastner D.B., Chuang J.Z., Yeh T.Y., Conde C.,
RA Caceres A., Sung C.H., Sakmar T.P.;
RT "G protein beta gamma subunit interaction with the dynein light-chain
RT component Tctex-1 regulates neurite outgrowth.";
RL EMBO J. 26:2621-2632(2007).
CC -!- FUNCTION: Acts as one of several non-catalytic accessory components of
CC the cytoplasmic dynein 1 complex that are thought to be involved in
CC linking dynein to cargos and to adapter proteins that regulate dynein
CC function. Cytoplasmic dynein 1 acts as a motor for the intracellular
CC retrograde motility of vesicles and organelles along microtubules.
CC Binds to transport cargos and is involved in apical cargo transport
CC such as rhodopsin-bearing vesicles in polarized epithelia. Is involved
CC in intracellular targeting of D-type retrovirus gag polyproteins to the
CC cytoplasmic assembly site. May also be a accessory component of
CC axonemal dynein (By similarity). {ECO:0000250}.
CC -!- FUNCTION: Plays a role in neuronal morphogenesis; the function is
CC independent of cytoplasmic dynein and seems to be coupled to regulation
CC of the actin cytoskeleton by enhancing Rac1 activity. Required for
CC neurite outgrowth. The function in neurogenesis may be regulated by
CC association with a G-protein beta-gamma dimer. May function as a
CC receptor-independent activator of heterotrimeric G-protein signaling;
CC the activation appears to be independent of a nucleotide exchange.
CC Plays a role in regulating neurogenesis; inhibits the genesis of
CC neurons from precursor cells during cortical development presumably by
CC antagonizing ARHGEF2. Unrelated to the role in retrograde microtubule-
CC associated movement may play a role in the dimerization of cytoplasmic
CC proteins/domains such as for ACVR2B. Binds to the cytoplasmic domain of
CC ACVR2B and, in vitro, inhibits ACVR2B signaling (By similarity).
CC Involved in the regulation of mitotic spindle orientation.
CC {ECO:0000250|UniProtKB:P63172, ECO:0000269|PubMed:15992542,
CC ECO:0000269|PubMed:17491591}.
CC -!- SUBUNIT: Homodimer (By similarity). The cytoplasmic dynein 1 complex
CC consists of two catalytic heavy chains (HCs) and a number of non-
CC catalytic subunits presented by intermediate chains (ICs), light
CC intermediate chains (LICs) and light chains (LCs); the composition
CC seems to vary in respect to the IC, LIC and LC composition. The heavy
CC chain homodimer serves as a scaffold for the probable homodimeric
CC assembly of the respective non-catalytic subunits. The ICs and LICs
CC bind directly to the HC dimer and dynein LCs assemble on the IC dimer.
CC DYNLT1 and DYNLT3 compete for association with dynein IC (DYNC1I1 or
CC DYNC1I2). Self-associates. Interacts with RHO. Interacts with DYNC1I1
CC and DYNC1I2 (By similarity). Interacts with DOC2A, DOC2B and SCN10A.
CC Interacts with PVR. Interacts with SVIL isoform 2 (By similarity).
CC Interacts with GNB1; the interaction occurs in presence of guanine
CC nucleotide-binding protein G(T) subunit gamma; the interaction
CC diminishes the association of DYNLT1 with dynein IC (DYNC1I1 or
CC DYNC1I2). Interacts with GNB2, GNB3 and GNB5; the interactions occur in
CC presence of guanine nucleotide-binding protein G(T) subunit gamma.
CC Interacts with ACVR2B and ARHGEF2 (By similarity). Interacts with DNAI4
CC (By similarity). {ECO:0000250, ECO:0000250|UniProtKB:P51807,
CC ECO:0000250|UniProtKB:P63172, ECO:0000269|PubMed:11746667,
CC ECO:0000269|PubMed:12591166, ECO:0000269|PubMed:17491591,
CC ECO:0000269|PubMed:9804756}.
CC -!- INTERACTION:
CC Q9Z336; Q62968: Scn10a; NbExp=2; IntAct=EBI-920359, EBI-1800320;
CC -!- SUBCELLULAR LOCATION: Golgi apparatus {ECO:0000250}. Cytoplasm
CC {ECO:0000250}. Cytoplasm, cytoskeleton, spindle {ECO:0000250}.
CC Note=Localizes to mitotic spindles. {ECO:0000250}.
CC -!- PTM: Phosphorylated by BMPR2. The phosphorylation status is proposed to
CC regulate the association with the cytoplasmic dynein complex and may
CC have role in cytoplasmic dynein cargo release.
CC -!- SIMILARITY: Belongs to the dynein light chain Tctex-type family.
CC {ECO:0000305}.
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DR EMBL; AB010119; BAA34532.1; -; mRNA.
DR EMBL; AJ131437; CAC18728.1; -; mRNA.
DR RefSeq; NP_112608.1; NM_031318.1.
DR AlphaFoldDB; Q9Z336; -.
DR SMR; Q9Z336; -.
DR BioGRID; 249705; 1.
DR CORUM; Q9Z336; -.
DR IntAct; Q9Z336; 2.
DR STRING; 10116.ENSRNOP00000024656; -.
DR iPTMnet; Q9Z336; -.
DR PhosphoSitePlus; Q9Z336; -.
DR jPOST; Q9Z336; -.
DR PaxDb; Q9Z336; -.
DR PRIDE; Q9Z336; -.
DR GeneID; 83462; -.
DR KEGG; rno:83462; -.
DR CTD; 6993; -.
DR RGD; 620261; Dynlt1.
DR VEuPathDB; HostDB:ENSRNOG00000018207; -.
DR eggNOG; KOG4081; Eukaryota.
DR HOGENOM; CLU_097204_7_2_1; -.
DR InParanoid; Q9Z336; -.
DR OMA; TSDACYV; -.
DR OrthoDB; 1474572at2759; -.
DR PhylomeDB; Q9Z336; -.
DR TreeFam; TF313904; -.
DR Reactome; R-RNO-6798695; Neutrophil degranulation.
DR PRO; PR:Q9Z336; -.
DR Proteomes; UP000002494; Chromosome 1.
DR Bgee; ENSRNOG00000018207; Expressed in ovary and 20 other tissues.
DR ExpressionAtlas; Q9Z336; baseline and differential.
DR Genevisible; Q9Z336; RN.
DR GO; GO:0044295; C:axonal growth cone; IDA:RGD.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005868; C:cytoplasmic dynein complex; ISS:UniProtKB.
DR GO; GO:0005881; C:cytoplasmic microtubule; ISO:RGD.
DR GO; GO:0030286; C:dynein complex; ISO:RGD.
DR GO; GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
DR GO; GO:0043657; C:host cell; IEA:GOC.
DR GO; GO:0030027; C:lamellipodium; IDA:RGD.
DR GO; GO:0005739; C:mitochondrion; IEA:GOC.
DR GO; GO:0043025; C:neuronal cell body; IDA:RGD.
DR GO; GO:0099503; C:secretory vesicle; ISO:RGD.
DR GO; GO:0005819; C:spindle; IEA:UniProtKB-SubCell.
DR GO; GO:0045505; F:dynein intermediate chain binding; IBA:GO_Central.
DR GO; GO:0042802; F:identical protein binding; ISO:RGD.
DR GO; GO:0008022; F:protein C-terminus binding; IPI:RGD.
DR GO; GO:0061564; P:axon development; IDA:RGD.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0016358; P:dendrite development; IDA:RGD.
DR GO; GO:0000132; P:establishment of mitotic spindle orientation; ISS:UniProtKB.
DR GO; GO:0006886; P:intracellular protein transport; TAS:UniProtKB.
DR GO; GO:0019060; P:intracellular transport of viral protein in host cell; ISO:RGD.
DR GO; GO:0007018; P:microtubule-based movement; IBA:GO_Central.
DR GO; GO:0060548; P:negative regulation of cell death; IMP:RGD.
DR GO; GO:0035795; P:negative regulation of mitochondrial membrane permeability; IMP:RGD.
DR GO; GO:0050768; P:negative regulation of neurogenesis; ISS:UniProtKB.
DR GO; GO:0048812; P:neuron projection morphogenesis; IMP:UniProtKB.
DR GO; GO:0010976; P:positive regulation of neuron projection development; IMP:RGD.
DR GO; GO:0035022; P:positive regulation of Rac protein signal transduction; IMP:RGD.
DR GO; GO:0051493; P:regulation of cytoskeleton organization; IDA:UniProtKB.
DR GO; GO:0008277; P:regulation of G protein-coupled receptor signaling pathway; ISS:UniProtKB.
DR GO; GO:0043087; P:regulation of GTPase activity; IMP:UniProtKB.
DR Gene3D; 3.30.1140.40; -; 1.
DR InterPro; IPR005334; Tctex-1-like.
DR InterPro; IPR038586; Tctex-1-like_sf.
DR PANTHER; PTHR21255; PTHR21255; 1.
DR Pfam; PF03645; Tctex-1; 1.
PE 1: Evidence at protein level;
KW Acetylation; Cell cycle; Cell division; Cytoplasm; Cytoskeleton; Dynein;
KW Golgi apparatus; Microtubule; Mitosis; Motor protein; Neurogenesis;
KW Phosphoprotein; Reference proteome; Transport.
FT CHAIN 1..113
FT /note="Dynein light chain Tctex-type 1"
FT /id="PRO_0000195154"
FT REGION 41..113
FT /note="Interaction with GNB1"
FT /evidence="ECO:0000269|PubMed:17491591"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000250|UniProtKB:P63172"
FT MUTAGEN 94
FT /note="T->A: No effect on interaction with dynein
FT intermediate chain (DYNC1I1 or DYNC1I2)."
FT /evidence="ECO:0000269|PubMed:15992542"
FT MUTAGEN 94
FT /note="T->E: Impairs interaction with dynein intermediate
FT chain (DYNC1I1 or DYNC1I2)."
FT /evidence="ECO:0000269|PubMed:15992542"
SQ SEQUENCE 113 AA; 12452 MW; 722890BD6832F041 CRC64;
MEDFQASEET AFVVDEVSNI VKEAIESAIG GNAYQHSKVN QWTTNVVEQT LSQLTKLGKP
FKYIVTCVIM QKNGAGLHTA SSCFWDSSTD GSCTVRWENK TMYCIVSAFG LSI
