DYLT2_MOUSE
ID DYLT2_MOUSE Reviewed; 191 AA.
AC P11985; P51806; Q66L72; Q7TN76; Q9D9X4;
DT 01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 2.
DT 03-AUG-2022, entry version 172.
DE RecName: Full=Dynein light chain Tctex-type protein 2 {ECO:0000305};
DE AltName: Full=LC2;
DE AltName: Full=T-complex testis-specific protein 2;
DE AltName: Full=T-complex testis-specific protein 3;
DE AltName: Full=T-complex-associated testis-expressed protein 3;
DE Short=Tcte-3;
DE AltName: Full=T-complex-associated testis-expressed protein 4;
DE Short=TCTEX-4;
DE AltName: Full=TCTEX-2;
DE AltName: Full=Tctex1 domain-containing protein 3;
GN Name=Dynlt2; Synonyms=Tcte3, Tctex1d3, Tctex2, Tctex4;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND TISSUE SPECIFICITY.
RC TISSUE=Testis;
RX PubMed=3653077; DOI=10.1002/j.1460-2075.1987.tb02460.x;
RA Rappold G.A., Stubbs L., Labeit S., Crkvenjakov R.B., Lehrach H.;
RT "Identification of a testis-specific gene from the mouse T-complex next to
RT a CpG-rich island.";
RL EMBO J. 6:1975-1980(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), SUBCELLULAR LOCATION, AND
RP TISSUE SPECIFICITY.
RC TISSUE=Testis;
RX PubMed=7601308; DOI=10.1006/dbio.1995.1206;
RA Huw L.-Y., Goldsborough A.S., Willison K., Artzt K.;
RT "Tctex2: a sperm tail surface protein mapping to the T-complex.";
RL Dev. Biol. 170:183-194(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), INTERACTION WITH CSNK2B, AND TISSUE
RP SPECIFICITY.
RC TISSUE=Testis;
RX PubMed=12849985; DOI=10.1016/s0006-291x(03)01118-5;
RA Bai X., Xu X., Zhang F., Shu H.-B., Chan E.D.;
RT "The protein of a new gene, Tctex4, interacts with protein kinase CK2beta
RT subunit and is highly expressed in mouse testis.";
RL Biochem. Biophys. Res. Commun. 307:86-91(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANT ARG-125.
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT ARG-125.
RC TISSUE=Brain, and Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP TISSUE SPECIFICITY.
RX PubMed=1718647; DOI=10.1002/dvg.1020120409;
RA Ha H., Howard C.A., Yeom Y.I., Abe K., Uehara H., Artzt K., Bennett D.;
RT "Several testis-expressed genes in the mouse T-complex have expression
RT differences between wild-type and t-mutant mice.";
RL Dev. Genet. 12:318-332(1991).
RN [7]
RP TISSUE SPECIFICITY, AND POSSIBLE FUNCTION IN AXONEMAL DYNEIN AND
RP CYTOPLASMIC DYNEIN 1.
RX PubMed=11278908; DOI=10.1074/jbc.m011456200;
RA DiBella L.M., Benashski S.E., Tedford H.W., Harrison A., Patel-King R.S.,
RA King S.M.;
RT "The Tctex1/Tctex2 class of dynein light chains. Dimerization, differential
RT expression, and interaction with the LC8 protein family.";
RL J. Biol. Chem. 276:14366-14373(2001).
RN [8]
RP VARIANT [LARGE SCALE ANALYSIS] ARG-125, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: May be an accessory component of axonemal dynein and
CC cytoplasmic dynein 1. Candidate for involvement in male sterility.
CC {ECO:0000269|PubMed:11278908}.
CC -!- SUBUNIT: Interacts with CSNK2B. {ECO:0000269|PubMed:12849985}.
CC -!- INTERACTION:
CC P11985-2; P67871: Csnk2b; NbExp=2; IntAct=EBI-1781298, EBI-348179;
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton
CC {ECO:0000305|PubMed:7601308}. Cytoplasmic granule
CC {ECO:0000269|PubMed:7601308}. Membrane {ECO:0000269|PubMed:7601308};
CC Peripheral membrane protein {ECO:0000269|PubMed:7601308}. Note=Found on
CC the surface of sperm tail. Stored in cytoplasmic granules during
CC spermatogenesis.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=Long;
CC IsoId=P11985-1; Sequence=Displayed;
CC Name=2; Synonyms=Short;
CC IsoId=P11985-2; Sequence=VSP_004449;
CC -!- TISSUE SPECIFICITY: Expressed in testis (at protein level). Expressed
CC at the pachyten stage of the first meiotic division and in later
CC haploid spermatogenic stages. {ECO:0000269|PubMed:11278908,
CC ECO:0000269|PubMed:12849985, ECO:0000269|PubMed:1718647,
CC ECO:0000269|PubMed:3653077, ECO:0000269|PubMed:7601308}.
CC -!- DISEASE: Note=Could be involved in transmission ratio distortion (trd)
CC in mouse t-haplotype which causes male sterility.
CC -!- SIMILARITY: Belongs to the dynein light chain Tctex-type family.
CC {ECO:0000305}.
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DR EMBL; M26332; AAA40413.1; -; mRNA.
DR EMBL; U21673; AAA81010.1; -; mRNA.
DR EMBL; U21674; AAA81011.1; -; mRNA.
DR EMBL; AK006370; BAB24552.1; -; mRNA.
DR EMBL; AY172988; AAO34134.1; -; mRNA.
DR EMBL; BC061136; AAH61136.1; -; mRNA.
DR EMBL; BC131981; AAI31982.1; -; mRNA.
DR EMBL; BC131983; AAI31984.1; -; mRNA.
DR CCDS; CCDS28408.1; -. [P11985-1]
DR CCDS; CCDS28409.1; -. [P11985-2]
DR PIR; A29687; A29687.
DR PIR; I49277; I49277.
DR PIR; I49278; I49278.
DR RefSeq; NP_001116839.1; NM_001123367.1.
DR RefSeq; NP_001116840.1; NM_001123368.1. [P11985-1]
DR RefSeq; NP_035690.2; NM_011560.3.
DR RefSeq; NP_932772.1; NM_198104.2. [P11985-2]
DR RefSeq; XP_006523432.1; XM_006523369.1.
DR RefSeq; XP_006523439.1; XM_006523376.1.
DR AlphaFoldDB; P11985; -.
DR SMR; P11985; -.
DR BioGRID; 204081; 1.
DR IntAct; P11985; 1.
DR STRING; 10090.ENSMUSP00000095011; -.
DR iPTMnet; P11985; -.
DR PhosphoSitePlus; P11985; -.
DR PaxDb; P11985; -.
DR PeptideAtlas; P11985; -.
DR PRIDE; P11985; -.
DR ProteomicsDB; 263017; -. [P11985-1]
DR ProteomicsDB; 263018; -. [P11985-2]
DR DNASU; 21647; -.
DR Ensembl; ENSMUST00000097398; ENSMUSP00000095010; ENSMUSG00000079707. [P11985-2]
DR Ensembl; ENSMUST00000097399; ENSMUSP00000095011; ENSMUSG00000116780. [P11985-1]
DR Ensembl; ENSMUST00000231584; ENSMUSP00000155901; ENSMUSG00000116780. [P11985-2]
DR GeneID; 100041586; -.
DR GeneID; 100041639; -.
DR GeneID; 21647; -.
DR KEGG; mmu:100041586; -.
DR KEGG; mmu:100041639; -.
DR KEGG; mmu:21647; -.
DR UCSC; uc008anl.2; mouse. [P11985-1]
DR UCSC; uc008ant.2; mouse. [P11985-2]
DR CTD; 100041586; -.
DR CTD; 100041639; -.
DR CTD; 21647; -.
DR MGI; MGI:98642; Tcte3.
DR VEuPathDB; HostDB:ENSMUSG00000079707; -.
DR VEuPathDB; HostDB:ENSMUSG00000116780; -.
DR eggNOG; KOG4108; Eukaryota.
DR GeneTree; ENSGT00940000160069; -.
DR HOGENOM; CLU_097204_0_0_1; -.
DR InParanoid; P11985; -.
DR OMA; IMIERVP; -.
DR OrthoDB; 1456915at2759; -.
DR PhylomeDB; P11985; -.
DR TreeFam; TF313904; -.
DR Reactome; R-MMU-5620924; Intraflagellar transport.
DR BioGRID-ORCS; 100041586; 2 hits in 31 CRISPR screens.
DR BioGRID-ORCS; 100041639; 0 hits in 12 CRISPR screens.
DR BioGRID-ORCS; 21647; 1 hit in 28 CRISPR screens.
DR ChiTaRS; Tcte3; mouse.
DR PRO; PR:P11985; -.
DR Proteomes; UP000000589; Chromosome 17.
DR RNAct; P11985; protein.
DR Bgee; ENSMUSG00000079707; Expressed in spermatid and 55 other tissues.
DR ExpressionAtlas; P11985; differential.
DR Genevisible; P11985; MM.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005868; C:cytoplasmic dynein complex; IDA:MGI.
DR GO; GO:0005829; C:cytosol; IDA:MGI.
DR GO; GO:0019898; C:extrinsic component of membrane; IDA:MGI.
DR GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR GO; GO:0036126; C:sperm flagellum; IDA:MGI.
DR GO; GO:0045505; F:dynein intermediate chain binding; IBA:GO_Central.
DR GO; GO:0007018; P:microtubule-based movement; IDA:MGI.
DR Gene3D; 3.30.1140.40; -; 1.
DR InterPro; IPR005334; Tctex-1-like.
DR InterPro; IPR038586; Tctex-1-like_sf.
DR PANTHER; PTHR21255; PTHR21255; 1.
DR Pfam; PF03645; Tctex-1; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Cytoskeleton; Disease variant; Dynein;
KW Membrane; Microtubule; Motor protein; Reference proteome; Transport.
FT CHAIN 1..191
FT /note="Dynein light chain Tctex-type protein 2"
FT /id="PRO_0000072471"
FT REGION 1..29
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 104..155
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12849985,
FT ECO:0000303|PubMed:7601308"
FT /id="VSP_004449"
FT VARIANT 17
FT /note="Q -> E (in T-haplotype)"
FT VARIANT 23
FT /note="R -> S (in T-haplotype)"
FT VARIANT 26
FT /note="K -> R (in T-haplotype)"
FT VARIANT 41..43
FT /note="ERL -> P (in T-haplotype)"
FT VARIANT 48
FT /note="H -> R (in T-haplotype)"
FT VARIANT 52
FT /note="Y -> F (in T-haplotype)"
FT VARIANT 60
FT /note="S -> A (in T-haplotype)"
FT VARIANT 64
FT /note="V -> S (requires 2 nucleotide substitutions; in T-
FT haplotype)"
FT VARIANT 93
FT /note="L -> S (in T-haplotype)"
FT VARIANT 125
FT /note="G -> R (in T-haplotype)"
FT /evidence="ECO:0000269|PubMed:15489334,
FT ECO:0000269|PubMed:16141072, ECO:0007744|PubMed:21183079"
FT VARIANT 153
FT /note="A -> G (in T-haplotype)"
FT CONFLICT 6..8
FT /note="RMA -> QLR (in Ref. 1; AAA40413)"
FT /evidence="ECO:0000305"
FT CONFLICT 98
FT /note="I -> V (in Ref. 1; AAA40413)"
FT /evidence="ECO:0000305"
FT CONFLICT 125..191
FT /note="GILAAVKEFAYHRYKFIIQVLFIQKTGQAINIASRWIWDVAWDNWVEAKHET
FT ESYVVLALVFALYCE -> QYWQQSKNFIPWIYYYTSIIYSKDWSSNKYCQQMDLGCGM
FT GQLGRS (in Ref. 1; AAA40413)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 191 AA; 22379 MW; 4BD9880898FE1DBD CRC64;
MERRGRMAKT PTGQTHQSPV SKRERKPSMF EKESYAQILR ERLRESFHDV QYVEPPFDDS
IADVGKEWKS ALAKLKFANS YRMEPLKKFQ AHLVETKIQQ ILKDSLKDVK YDDKAPHLSL
ELADGILAAV KEFAYHRYKF IIQVLFIQKT GQAINIASRW IWDVAWDNWV EAKHETESYV
VLALVFALYC E
