DYLT3_MOUSE
ID DYLT3_MOUSE Reviewed; 116 AA.
AC P56387;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 15-JUL-1998, sequence version 1.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Dynein light chain Tctex-type 3;
DE AltName: Full=Protein 91/23;
DE AltName: Full=T-complex-associated testis-expressed 1-like;
GN Name=Dynlt3; Synonyms=Tcte1l;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 1-114 AND 23-116.
RA Marra M., Hillier L., Allen M., Bowles M., Dietrich N., Dubuque T.,
RA Geisel S., Kucaba T., Lacy M., Le M., Martin J., Morris M.,
RA Schellenberg K., Steptoe M., Tan F., Underwood K., Moore B., Theising B.,
RA Wylie T., Lennon G., Soares B., Wilson R., Waterston R.;
RL Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NITRATION [LARGE SCALE ANALYSIS] AT TYR-4, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain;
RX PubMed=16800626; DOI=10.1021/bi060474w;
RA Sacksteder C.A., Qian W.-J., Knyushko T.V., Wang H., Chin M.H., Lacan G.,
RA Melega W.P., Camp D.G. II, Smith R.D., Smith D.J., Squier T.C.,
RA Bigelow D.J.;
RT "Endogenously nitrated proteins in mouse brain: links to neurodegenerative
RT disease.";
RL Biochemistry 45:8009-8022(2006).
RN [4]
RP INTERACTION WITH BUB3.
RX PubMed=17289665; DOI=10.1074/jbc.m611279200;
RA Lo K.W., Kogoy J.M., Pfister K.K.;
RT "The DYNLT3 light chain directly links cytoplasmic dynein to a spindle
RT checkpoint protein, Bub3.";
RL J. Biol. Chem. 282:11205-11212(2007).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas,
RC and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Acts as one of several non-catalytic accessory components of
CC the cytoplasmic dynein 1 complex that are thought to be involved in
CC linking dynein to cargos and to adapter proteins that regulate dynein
CC function. Cytoplasmic dynein 1 acts as a motor for the intracellular
CC retrograde motility of vesicles and organelles along microtubules.
CC Probably binds BUB3 as part of transport cargo. Required for the
CC efficient progression through mitosis.
CC -!- SUBUNIT: Homodimer. The cytoplasmic dynein 1 complex consists of two
CC catalytic heavy chains (HCs) and a number of non-catalytic subunits
CC presented by intermediate chains (ICs), light intermediate chains
CC (LICs) and light chains (LCs); the composition seems to vary in respect
CC to the IC, LIC and LC composition. The heavy chain homodimer serves as
CC a scaffold for the probable homodimeric assembly of the respective non-
CC catalytic subunits. The ICs and LICs bind directly to the HC dimer and
CC the LCs assemble on the IC dimer. DYNLT1 and DYNLT3 compete for
CC association with dynein IC (DYNC1I1 or DYNC1I2). Self-associates.
CC Interacts with DYNC1I1 and DYNC1I2. Interacts with BUB3. Interacts with
CC SATB1 in nucleus to form complex with matrix attachment regions (MARs)
CC of DNA (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Cytoplasm, cytoskeleton
CC {ECO:0000250}. Chromosome, centromere, kinetochore {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the dynein light chain Tctex-type family.
CC {ECO:0000305}.
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DR EMBL; BC030032; AAH30032.1; -; mRNA.
DR EMBL; AA636653; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AA516811; -; NOT_ANNOTATED_CDS; mRNA.
DR CCDS; CCDS30011.1; -.
DR RefSeq; NP_080251.3; NM_025975.5.
DR AlphaFoldDB; P56387; -.
DR SMR; P56387; -.
DR BioGRID; 211951; 18.
DR IntAct; P56387; 3.
DR MINT; P56387; -.
DR STRING; 10090.ENSMUSP00000033519; -.
DR iPTMnet; P56387; -.
DR PhosphoSitePlus; P56387; -.
DR EPD; P56387; -.
DR PaxDb; P56387; -.
DR PeptideAtlas; P56387; -.
DR PRIDE; P56387; -.
DR ProteomicsDB; 275418; -.
DR Antibodypedia; 10567; 95 antibodies from 22 providers.
DR DNASU; 67117; -.
DR Ensembl; ENSMUST00000033519; ENSMUSP00000033519; ENSMUSG00000031176.
DR GeneID; 67117; -.
DR KEGG; mmu:67117; -.
DR UCSC; uc009spy.1; mouse.
DR CTD; 6990; -.
DR MGI; MGI:1914367; Dynlt3.
DR VEuPathDB; HostDB:ENSMUSG00000031176; -.
DR eggNOG; KOG4081; Eukaryota.
DR GeneTree; ENSGT00940000155009; -.
DR HOGENOM; CLU_097204_7_0_1; -.
DR InParanoid; P56387; -.
DR OMA; LWENSTI; -.
DR OrthoDB; 1474572at2759; -.
DR PhylomeDB; P56387; -.
DR TreeFam; TF313904; -.
DR BioGRID-ORCS; 67117; 2 hits in 71 CRISPR screens.
DR ChiTaRS; Dynlt3; mouse.
DR PRO; PR:P56387; -.
DR Proteomes; UP000000589; Chromosome X.
DR RNAct; P56387; protein.
DR Bgee; ENSMUSG00000031176; Expressed in choroid plexus epithelium and 269 other tissues.
DR ExpressionAtlas; P56387; baseline and differential.
DR Genevisible; P56387; MM.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0005868; C:cytoplasmic dynein complex; ISS:UniProtKB.
DR GO; GO:0000776; C:kinetochore; ISO:MGI.
DR GO; GO:0061673; C:mitotic spindle astral microtubule; ISO:MGI.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0045505; F:dynein intermediate chain binding; IBA:GO_Central.
DR GO; GO:0042802; F:identical protein binding; ISO:MGI.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007018; P:microtubule-based movement; IBA:GO_Central.
DR GO; GO:0045931; P:positive regulation of mitotic cell cycle; ISO:MGI.
DR GO; GO:0007346; P:regulation of mitotic cell cycle; ISS:UniProtKB.
DR Gene3D; 3.30.1140.40; -; 1.
DR InterPro; IPR005334; Tctex-1-like.
DR InterPro; IPR038586; Tctex-1-like_sf.
DR PANTHER; PTHR21255; PTHR21255; 1.
DR Pfam; PF03645; Tctex-1; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Centromere; Chromosome; Cytoplasm; Cytoskeleton;
KW Dynein; Kinetochore; Microtubule; Mitosis; Motor protein; Nitration;
KW Nucleus; Reference proteome; Transport.
FT CHAIN 1..116
FT /note="Dynein light chain Tctex-type 3"
FT /id="PRO_0000195159"
FT MOD_RES 4
FT /note="3'-nitrotyrosine"
FT /evidence="ECO:0007744|PubMed:16800626"
SQ SEQUENCE 116 AA; 12958 MW; 843D22BE1043644A CRC64;
MEGYQRPCDE VGFNADEAHN IVKECVDGVL GGNDYNENNI NQWTASIVEQ SITHLVKLGK
AYKYIVTCAV VQRSPYGFHT ASSCFWDTTS DGTCTIRWEN RTMNCIVNVF AVAIVL
