DYLT_DROME
ID DYLT_DROME Reviewed; 111 AA.
AC Q94524; Q9VE95;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 160.
DE RecName: Full=Dynein light chain Tctex-type;
DE AltName: Full=TCTEX-1 protein homolog;
GN Name=Dlc90F; Synonyms=Tctex; ORFNames=CG12363;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Ovary;
RX PubMed=10071211; DOI=10.1007/s004380050942;
RA Caggese C., Ragone G., Perrini B., Moschetti R., de Pinto V., Caizzi R.,
RA Barsanti P.;
RT "Identification of nuclear genes encoding mitochondrial proteins: isolation
RT of a collection of D. melanogaster cDNAs homologous to sequences in the
RT Human Gene Index database.";
RL Mol. Gen. Genet. 261:64-70(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=11405626; DOI=10.1007/s004380000431;
RA Caggese C., Moschetti R., Ragone G., Barsanti P., Caizzi R.;
RT "dtctex-1, the Drosophila melanogaster homolog of a putative murine t-
RT complex distorter encoding a dynein light chain, is required for production
RT of functional sperm.";
RL Mol. Genet. Genomics 265:436-444(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RA Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M.,
RA Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A.,
RA Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A.,
RA Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S.,
RA Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E.;
RL Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP SUBUNIT, FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=15090621; DOI=10.1091/mbc.e04-01-0013;
RA Li M.G., Serr M., Newman E.A., Hays T.S.;
RT "The Drosophila tctex-1 light chain is dispensable for essential
RT cytoplasmic dynein functions but is required during spermatid
RT differentiation.";
RL Mol. Biol. Cell 15:3005-3014(2004).
CC -!- FUNCTION: Acts as one of several non-catalytic accessory components of
CC the cytoplasmic dynein complex that are thought to be involved in
CC linking dynein to cargos and to adapter proteins that regulate dynein
CC function. Cytoplasmic dynein acts as a motor for the intracellular
CC retrograde motility of vesicles and organelles along microtubules.
CC Required for spermatid differentiation. Is not required for polarized
CC transport in rhabdomere development and appears to be a non-essential
CC component of the cytoplasmic dynein complex.
CC {ECO:0000269|PubMed:15090621}.
CC -!- SUBUNIT: The cytoplasmic dynein complex consists of two catalytic heavy
CC chains (HCs) and a number of non-catalytic subunits presented by
CC intermediate chains (ICs), light intermediate chains (LICs) and light
CC chains (LCs). {ECO:0000250}.
CC -!- INTERACTION:
CC Q94524; Q94524: Dlc90F; NbExp=3; IntAct=EBI-158251, EBI-158251;
CC Q94524; Q9VH45: Dmel\CG5359; NbExp=4; IntAct=EBI-158251, EBI-155608;
CC Q94524; P22979: Hsp67Bc; NbExp=6; IntAct=EBI-158251, EBI-165408;
CC Q94524; Q9VIT3: msb1l; NbExp=3; IntAct=EBI-158251, EBI-121559;
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton {ECO:0000305}.
CC -!- DISRUPTION PHENOTYPE: Male sterility. {ECO:0000269|PubMed:15090621}.
CC -!- SIMILARITY: Belongs to the dynein light chain Tctex-type family.
CC {ECO:0000305}.
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DR EMBL; Y08968; CAA70165.1; -; mRNA.
DR EMBL; AF123058; AAD30033.1; -; Genomic_DNA.
DR EMBL; AE014297; AAF55532.1; -; Genomic_DNA.
DR EMBL; AY069608; AAL39753.1; -; mRNA.
DR RefSeq; NP_477356.1; NM_058008.4.
DR PDB; 1YGT; X-ray; 1.70 A; A=1-111.
DR PDB; 2PG1; X-ray; 2.80 A; E/F/G/H=1-111.
DR PDB; 3FM7; X-ray; 3.50 A; A/B=1-111.
DR PDBsum; 1YGT; -.
DR PDBsum; 2PG1; -.
DR PDBsum; 3FM7; -.
DR AlphaFoldDB; Q94524; -.
DR SMR; Q94524; -.
DR BioGRID; 67217; 48.
DR DIP; DIP-17339N; -.
DR IntAct; Q94524; 24.
DR MINT; Q94524; -.
DR STRING; 7227.FBpp0082996; -.
DR PaxDb; Q94524; -.
DR PRIDE; Q94524; -.
DR DNASU; 42199; -.
DR EnsemblMetazoa; FBtr0083575; FBpp0082996; FBgn0024432.
DR GeneID; 42199; -.
DR KEGG; dme:Dmel_CG12363; -.
DR CTD; 42199; -.
DR FlyBase; FBgn0024432; Dlc90F.
DR VEuPathDB; VectorBase:FBgn0024432; -.
DR eggNOG; KOG4081; Eukaryota.
DR GeneTree; ENSGT00940000154531; -.
DR HOGENOM; CLU_097204_7_2_1; -.
DR InParanoid; Q94524; -.
DR OMA; TSDACYV; -.
DR OrthoDB; 1474572at2759; -.
DR PhylomeDB; Q94524; -.
DR Reactome; R-DME-6798695; Neutrophil degranulation.
DR SignaLink; Q94524; -.
DR BioGRID-ORCS; 42199; 1 hit in 3 CRISPR screens.
DR EvolutionaryTrace; Q94524; -.
DR GenomeRNAi; 42199; -.
DR PRO; PR:Q94524; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0024432; Expressed in eye disc (Drosophila) and 35 other tissues.
DR ExpressionAtlas; Q94524; baseline and differential.
DR Genevisible; Q94524; DM.
DR GO; GO:1904115; C:axon cytoplasm; IEA:GOC.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005868; C:cytoplasmic dynein complex; ISS:FlyBase.
DR GO; GO:0030286; C:dynein complex; IDA:FlyBase.
DR GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR GO; GO:0032991; C:protein-containing complex; IDA:CAFA.
DR GO; GO:0097718; F:disordered domain specific binding; IPI:CAFA.
DR GO; GO:0045505; F:dynein intermediate chain binding; IPI:FlyBase.
DR GO; GO:0051959; F:dynein light intermediate chain binding; IDA:FlyBase.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0042803; F:protein homodimerization activity; IDA:FlyBase.
DR GO; GO:0008340; P:determination of adult lifespan; IMP:FlyBase.
DR GO; GO:0007018; P:microtubule-based movement; IBA:GO_Central.
DR GO; GO:0000278; P:mitotic cell cycle; HMP:FlyBase.
DR GO; GO:0008090; P:retrograde axonal transport; IMP:FlyBase.
DR GO; GO:0007286; P:spermatid development; IMP:FlyBase.
DR Gene3D; 3.30.1140.40; -; 1.
DR IDEAL; IID50099; -.
DR InterPro; IPR005334; Tctex-1-like.
DR InterPro; IPR038586; Tctex-1-like_sf.
DR PANTHER; PTHR21255; PTHR21255; 1.
DR Pfam; PF03645; Tctex-1; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Cytoskeleton; Dynein; Microtubule; Motor protein;
KW Reference proteome.
FT CHAIN 1..111
FT /note="Dynein light chain Tctex-type"
FT /id="PRO_0000195155"
FT HELIX 14..27
FT /evidence="ECO:0007829|PDB:1YGT"
FT HELIX 34..53
FT /evidence="ECO:0007829|PDB:1YGT"
FT TURN 54..56
FT /evidence="ECO:0007829|PDB:3FM7"
FT STRAND 58..69
FT /evidence="ECO:0007829|PDB:1YGT"
FT STRAND 75..83
FT /evidence="ECO:0007829|PDB:2PG1"
FT TURN 85..87
FT /evidence="ECO:0007829|PDB:1YGT"
FT STRAND 89..96
FT /evidence="ECO:0007829|PDB:1YGT"
FT STRAND 98..110
FT /evidence="ECO:0007829|PDB:1YGT"
SQ SEQUENCE 111 AA; 12479 MW; 544CC39F5B318137 CRC64;
MDDSREESQF IVDDVSKTIK EAIETTIGGN AYQHDKVNNW TGQVVENCLT VLTKEQKPYK
YIVTAMIMQK NGAGLHTASS CYWNNDTDGS CTVRWENKTM YCIVSVFGLA V