DYM_MOUSE
ID DYM_MOUSE Reviewed; 669 AA.
AC Q8CHY3; Q3U3F3; Q3UDW2; Q569W1; Q8C7M2; Q8VCZ4;
DT 05-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Dymeclin;
GN Name=Dym;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J, and NOD; TISSUE=Bone marrow, and Hippocampus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=FVB/N; TISSUE=Mammary tumor, and Salivary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Liver, Lung, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Necessary for correct organization of Golgi apparatus.
CC Involved in bone development. {ECO:0000250}.
CC -!- SUBUNIT: Interacts with GOLM1 and PPIB. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm. Golgi apparatus {ECO:0000250}.
CC Membrane {ECO:0000250|UniProtKB:Q7RTS9}; Lipid-anchor
CC {ECO:0000250|UniProtKB:Q7RTS9}. Note=Sequence analysis programs predict
CC 1 transmembrane region. However, it has been shown in human that it is
CC not a stably anchored transmembrane protein but it weakly associates
CC with the Golgi apparatus and shuttles between the Golgi and the cytosol
CC (By similarity). {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8CHY3-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8CHY3-2; Sequence=VSP_036444, VSP_036445;
CC -!- PTM: Myristoylated in vitro; myristoylation is not essential for
CC protein targeting to Golgi compartment. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the dymeclin family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH18220.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=BAC33983.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=BAE32833.1; Type=Erroneous termination; Note=Truncated C-terminus.; Evidence={ECO:0000305};
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DR EMBL; AK049909; BAC33983.1; ALT_INIT; mRNA.
DR EMBL; AK149628; BAE28996.1; -; mRNA.
DR EMBL; AK149891; BAE29149.1; -; mRNA.
DR EMBL; AK154794; BAE32833.1; ALT_SEQ; mRNA.
DR EMBL; BC018220; AAH18220.1; ALT_INIT; mRNA.
DR EMBL; BC038276; AAH38276.1; -; mRNA.
DR EMBL; BC092283; AAH92283.1; -; mRNA.
DR CCDS; CCDS29346.1; -. [Q8CHY3-1]
DR RefSeq; NP_082003.1; NM_027727.2. [Q8CHY3-1]
DR RefSeq; XP_006526282.1; XM_006526219.3.
DR AlphaFoldDB; Q8CHY3; -.
DR BioGRID; 213282; 1.
DR STRING; 10090.ENSMUSP00000047054; -.
DR iPTMnet; Q8CHY3; -.
DR PhosphoSitePlus; Q8CHY3; -.
DR EPD; Q8CHY3; -.
DR MaxQB; Q8CHY3; -.
DR PaxDb; Q8CHY3; -.
DR PeptideAtlas; Q8CHY3; -.
DR PRIDE; Q8CHY3; -.
DR ProteomicsDB; 275419; -. [Q8CHY3-1]
DR ProteomicsDB; 275420; -. [Q8CHY3-2]
DR Antibodypedia; 22572; 134 antibodies from 22 providers.
DR DNASU; 69190; -.
DR Ensembl; ENSMUST00000039608; ENSMUSP00000047054; ENSMUSG00000035765. [Q8CHY3-1]
DR GeneID; 69190; -.
DR KEGG; mmu:69190; -.
DR UCSC; uc008fpz.1; mouse. [Q8CHY3-1]
DR CTD; 54808; -.
DR MGI; MGI:1918480; Dym.
DR VEuPathDB; HostDB:ENSMUSG00000035765; -.
DR eggNOG; KOG2225; Eukaryota.
DR GeneTree; ENSGT00390000008772; -.
DR HOGENOM; CLU_013309_2_0_1; -.
DR InParanoid; Q8CHY3; -.
DR OMA; WNGLLNY; -.
DR OrthoDB; 982213at2759; -.
DR PhylomeDB; Q8CHY3; -.
DR TreeFam; TF314870; -.
DR BioGRID-ORCS; 69190; 4 hits in 72 CRISPR screens.
DR ChiTaRS; Dym; mouse.
DR PRO; PR:Q8CHY3; -.
DR Proteomes; UP000000589; Chromosome 18.
DR RNAct; Q8CHY3; protein.
DR Bgee; ENSMUSG00000035765; Expressed in spermatocyte and 263 other tissues.
DR ExpressionAtlas; Q8CHY3; baseline and differential.
DR Genevisible; Q8CHY3; MM.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0005794; C:Golgi apparatus; ISS:UniProtKB.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0019899; F:enzyme binding; ISO:MGI.
DR GO; GO:0060348; P:bone development; ISS:UniProtKB.
DR GO; GO:0007030; P:Golgi organization; ISS:UniProtKB.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR019142; Dymeclin.
DR PANTHER; PTHR12895; PTHR12895; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Golgi apparatus; Lipoprotein; Membrane;
KW Myristate; Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:Q7RTS9"
FT CHAIN 2..669
FT /note="Dymeclin"
FT /id="PRO_0000086884"
FT LIPID 2
FT /note="N-myristoyl glycine"
FT /evidence="ECO:0000250|UniProtKB:Q7RTS9"
FT VAR_SEQ 211..216
FT /note="TSKLVK -> PFSPGV (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_036444"
FT VAR_SEQ 217..669
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_036445"
FT CONFLICT 313
FT /note="N -> Y (in Ref. 1; BAE32833)"
FT /evidence="ECO:0000305"
FT CONFLICT 505
FT /note="Q -> K (in Ref. 2; AAH18220)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 669 AA; 75848 MW; 47B7200E0E8AD20B CRC64;
MGSNSSKISD LPKNEYLKKL SGPESISEND PFWNQLFSFS FPAPTSSSEL KLLEEATISV
CKSLVENNPR TGNLAALTKV FLSRTRELRL SAECQNHIFI WQTHNALFII CCLLKVFIRE
LSEEELQLHF TYEEKSPGSY SSDSEDLLEE LLCSLVQLIT DTPLLDITYE IAVEAISAMV
VFLSCQLFHK EVLRQSISHK YLMQGPCLPY TSKLVKTLLY NFIRQEKPPP PGTHVFPQQS
DGGGLLYGLA SGVATGLWTV FTLGGAGSKA AASPELTSPL ANQSLLLLLV LVNLTDAPDI
PNPYRQAVSS FKNTQDSSPF PSSIPHTFQI NFNSLYTALC EQQTSDQATL LLYTLLHQNS
NVRTYVLART DMENLVLPIL EILYHVEERN SHHVYMALII LLILTEDDGF NRSIHEVILR
NITWYSERVL TEISLGSLLI LVVIRTIQYN MTRTRDKYLH TNCLAALANM SAQFRSLHQY
AAQRIISLFS LLSKKHNKVL EQATQSLRGP LSSSDVPLPD YAQDLSVIEE VIRMMLEIIN
SCLTNSLHHN PNLVYALLYK RDLFEQFRTH PSFQDIMQNI DLVISFFSSR LLQAGAELSV
ERVLEIIKQG VVALPKDRLK KFPELKFKYV EEEQPEEFFI PYVWSLVYNS AVGLYWNPQD
IQLFAMDSD
