DYN3_YEAST
ID DYN3_YEAST Reviewed; 312 AA.
AC Q04949; D6W0C6; Q03592;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 2.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Cytoplasmic dynein intermediate light chain DYN3;
DE Short=Dynein protein 3;
GN Name=DYN3; OrderedLocusNames=YMR299C; ORFNames=YM9952.01C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169872;
RA Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T.,
RA Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K.,
RA Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P.,
RA Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.";
RL Nature 387:90-93(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [4]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, AND INTERACTION WITH DYN1.
RX PubMed=15642746; DOI=10.1083/jcb.200407036;
RA Lee W.-L., Kaiser M.A., Cooper J.A.;
RT "The offloading model for dynein function: differential function of motor
RT subunits.";
RL J. Cell Biol. 168:201-207(2005).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- FUNCTION: Component of the cytoplasmic dynein which acts as a motor for
CC the intracellular retrograde motility of vesicles and organelles along
CC microtubules. May play an important role in the proper orientation of
CC the mitotic spindle into the budding daughter cell yeast. Probably
CC required for normal progression of the cell cycle.
CC {ECO:0000269|PubMed:15642746}.
CC -!- SUBUNIT: The dynein complex consists of at least two heavy chains and a
CC number of intermediate and light chains. Interacts with DYN1.
CC {ECO:0000269|PubMed:15642746}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton
CC {ECO:0000269|PubMed:14562095, ECO:0000269|PubMed:15642746}.
CC Note=Concentrates at motile dots in the cytoplasm corresponding to the
CC plus ends of cytoplasmic microtubules.
CC -!- MISCELLANEOUS: Present with 1170 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the dynein light intermediate chain DYN3 family.
CC {ECO:0000305}.
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DR EMBL; X80836; CAA56808.1; -; Genomic_DNA.
DR EMBL; Z49212; CAA89132.1; -; Genomic_DNA.
DR EMBL; BK006946; DAA10200.2; -; Genomic_DNA.
DR PIR; S53969; S53969.
DR RefSeq; NP_014028.2; NM_001182808.2.
DR AlphaFoldDB; Q04949; -.
DR BioGRID; 35479; 295.
DR ComplexPortal; CPX-1178; Cytoplasmic dynein complex.
DR DIP; DIP-7271N; -.
DR IntAct; Q04949; 14.
DR MINT; Q04949; -.
DR STRING; 4932.YMR299C; -.
DR MaxQB; Q04949; -.
DR PaxDb; Q04949; -.
DR PRIDE; Q04949; -.
DR EnsemblFungi; YMR299C_mRNA; YMR299C; YMR299C.
DR GeneID; 855345; -.
DR KEGG; sce:YMR299C; -.
DR SGD; S000004914; DYN3.
DR VEuPathDB; FungiDB:YMR299C; -.
DR eggNOG; ENOG502S4QG; Eukaryota.
DR HOGENOM; CLU_064762_0_0_1; -.
DR InParanoid; Q04949; -.
DR OMA; QLERNTM; -.
DR BioCyc; YEAST:G3O-32966-MON; -.
DR PRO; PR:Q04949; -.
DR Proteomes; UP000002311; Chromosome XIII.
DR RNAct; Q04949; protein.
DR GO; GO:0005737; C:cytoplasm; IC:ComplexPortal.
DR GO; GO:0005868; C:cytoplasmic dynein complex; IPI:SGD.
DR GO; GO:0005881; C:cytoplasmic microtubule; IDA:SGD.
DR GO; GO:0040001; P:establishment of mitotic spindle localization; IC:ComplexPortal.
DR GO; GO:0000132; P:establishment of mitotic spindle orientation; IC:ComplexPortal.
DR GO; GO:0030473; P:nuclear migration along microtubule; IMP:SGD.
DR InterPro; IPR022780; Dynein_light_int_chain.
DR Pfam; PF05783; DLIC; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Cytoskeleton; Dynein; Microtubule; Motor protein;
KW Reference proteome.
FT CHAIN 1..312
FT /note="Cytoplasmic dynein intermediate light chain DYN3"
FT /id="PRO_0000203353"
SQ SEQUENCE 312 AA; 36825 MW; BFDEC080E08602A2 CRC64;
MDNCNAWDKL LSQNESTINS TETATITAII YSPSSKTLHQ FINICFPEGS NSILDTTLIN
YATIGWTNDL KENYSVDVYT LIRNTDDALD LLKPFLQEHS SKVRWLILLD WTLNDQKLWL
NELSYAFNKI KQLNDDNEFS VWCLNSGEIL NLQRHTTVWQ SVHIDFILQT LRSFCYFNDS
SLFYICEDHT EEKREEAQRL KYQELLKHFC EDRDMKDHIE MVKRSEILIP KGCDSIGLIK
TVDERFEPTE VKEQHFLARY MDFIPTIDKI REDRKTTSGI DLDKLYPLEV FKVNIQEELG
KMFAKYRENS RI
