DYNAP_HUMAN
ID DYNAP_HUMAN Reviewed; 210 AA.
AC Q8N1N2;
DT 16-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=Dynactin-associated protein;
DE Short=Full;
GN Name=DYNAP; Synonyms=C18orf26;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Tongue;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16177791; DOI=10.1038/nature03983;
RA Nusbaum C., Zody M.C., Borowsky M.L., Kamal M., Kodira C.D., Taylor T.D.,
RA Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X.,
RA Abouelleil A., Allen N.R., Anderson S., Bloom T., Bugalter B., Butler J.,
RA Cook A., DeCaprio D., Engels R., Garber M., Gnirke A., Hafez N., Hall J.L.,
RA Norman C.H., Itoh T., Jaffe D.B., Kuroki Y., Lehoczky J., Lui A.,
RA Macdonald P., Mauceli E., Mikkelsen T.S., Naylor J.W., Nicol R., Nguyen C.,
RA Noguchi H., O'Leary S.B., Piqani B., Smith C.L., Talamas J.A., Topham K.,
RA Totoki Y., Toyoda A., Wain H.M., Young S.K., Zeng Q., Zimmer A.R.,
RA Fujiyama A., Hattori M., Birren B.W., Sakaki Y., Lander E.S.;
RT "DNA sequence and analysis of human chromosome 18.";
RL Nature 437:551-555(2005).
RN [3]
RP FUNCTION, INTERACTION WITH DCTN1 AND DCTN2, TOPOLOGY, SUBCELLULAR LOCATION,
RP AND TISSUE SPECIFICITY.
RX PubMed=20978158; DOI=10.1158/1535-7163.mct-10-0730;
RA Kunoh T., Noda T., Koseki K., Sekigawa M., Takagi M., Shin-ya K.,
RA Goshima N., Iemura S., Natsume T., Wada S., Mukai Y., Ohta S., Sasaki R.,
RA Mizukami T.;
RT "A novel human dynactin-associated protein, dynAP, promotes activation of
RT Akt, and ergosterol-related compounds induce dynAP-dependent apoptosis of
RT human cancer cells.";
RL Mol. Cancer Ther. 9:2934-2942(2010).
CC -!- FUNCTION: Plays a role in the regulation of cell proliferation.
CC Promotes activation of the AKT1 signaling pathway. Promotes
CC phosphorylation of AKT1 at 'Ser-473'. {ECO:0000269|PubMed:20978158}.
CC -!- SUBUNIT: Interacts with DCTN1 and DCTN2. {ECO:0000269|PubMed:20978158}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000269|PubMed:20978158}; Single-pass membrane protein
CC {ECO:0000269|PubMed:20978158}. Cell membrane
CC {ECO:0000269|PubMed:20978158}; Single-pass membrane protein
CC {ECO:0000269|PubMed:20978158}. Note=Concentrated at cell-cell borders.
CC Localizes at membranes in a microtubule-dependent manner.
CC -!- TISSUE SPECIFICITY: Expressed in fibroblast and numerous cancer cell
CC lines (at protein level). {ECO:0000269|PubMed:20978158}.
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DR EMBL; AK096425; BAC04784.1; -; mRNA.
DR EMBL; AC091135; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; NP_775900.1; NM_173629.2.
DR AlphaFoldDB; Q8N1N2; -.
DR STRING; 9606.ENSP00000315265; -.
DR PhosphoSitePlus; Q8N1N2; -.
DR BioMuta; DYNAP; -.
DR DMDM; 73620598; -.
DR PaxDb; Q8N1N2; -.
DR PRIDE; Q8N1N2; -.
DR Antibodypedia; 2651; 67 antibodies from 13 providers.
DR DNASU; 284254; -.
DR Ensembl; ENST00000321600.1; ENSP00000315265.1; ENSG00000178690.4.
DR GeneID; 284254; -.
DR KEGG; hsa:284254; -.
DR UCSC; uc002lfq.1; human.
DR CTD; 284254; -.
DR DisGeNET; 284254; -.
DR GeneCards; DYNAP; -.
DR HGNC; HGNC:26808; DYNAP.
DR HPA; ENSG00000178690; Tissue enriched (esophagus).
DR MIM; 619421; gene.
DR neXtProt; NX_Q8N1N2; -.
DR OpenTargets; ENSG00000178690; -.
DR PharmGKB; PA134946407; -.
DR VEuPathDB; HostDB:ENSG00000178690; -.
DR eggNOG; ENOG502RTZD; Eukaryota.
DR GeneTree; ENSGT00400000022381; -.
DR HOGENOM; CLU_121237_0_0_1; -.
DR InParanoid; Q8N1N2; -.
DR OMA; YVVNIEH; -.
DR OrthoDB; 1534093at2759; -.
DR PhylomeDB; Q8N1N2; -.
DR TreeFam; TF338599; -.
DR PathwayCommons; Q8N1N2; -.
DR SignaLink; Q8N1N2; -.
DR BioGRID-ORCS; 284254; 7 hits in 1055 CRISPR screens.
DR ChiTaRS; DYNAP; human.
DR GenomeRNAi; 284254; -.
DR Pharos; Q8N1N2; Tbio.
DR PRO; PR:Q8N1N2; -.
DR Proteomes; UP000005640; Chromosome 18.
DR RNAct; Q8N1N2; protein.
DR Bgee; ENSG00000178690; Expressed in lower esophagus mucosa and 57 other tissues.
DR ExpressionAtlas; Q8N1N2; baseline and differential.
DR GO; GO:0005794; C:Golgi apparatus; IDA:UniProtKB.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR GO; GO:0032148; P:activation of protein kinase B activity; IDA:UniProtKB.
DR GO; GO:1901625; P:cellular response to ergosterol; IDA:UniProtKB.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; IDA:UniProtKB.
DR GO; GO:0042981; P:regulation of apoptotic process; IDA:UniProtKB.
DR InterPro; IPR031379; CLLAC.
DR Pfam; PF15675; CLLAC; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Golgi apparatus; Membrane; Reference proteome;
KW Signal-anchor; Transmembrane; Transmembrane helix.
FT CHAIN 1..210
FT /note="Dynactin-associated protein"
FT /id="PRO_0000079315"
FT TOPO_DOM 1..113
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 114..134
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 135..210
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT REGION 168..210
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 170..204
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 38
FT /note="V -> A (in dbSNP:rs35428499)"
FT /id="VAR_033751"
FT VARIANT 189
FT /note="T -> P (in dbSNP:rs9947055)"
FT /id="VAR_033752"
SQ SEQUENCE 210 AA; 22529 MW; E94FE6D340D3CF79 CRC64;
MVADIKGNEQ IEKYSWREAC DTGSSRMDRK HGKYILNVEH SENQPPITHP NDQEAHSSIC
WCLPSNDITS DVSPNLTGVC VNPGILAHSR CLQSESCNTQ VKEYCRNDWS MWKVFLACLL
ACVIMTAIGV LIICLVNNKG SANSSIVIQL STNDGECVTV KPGTPSPACP PTMTTTSTVP
ASTATESTTS TATAATTSTE PITVAPTDHL
