ID   DYNA_NEUCR              Reviewed;        1367 AA.
AC   Q01397; Q7RV43; Q8WZS2;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   04-DEC-2007, sequence version 3.
DT   25-MAY-2022, entry version 136.
DE   RecName: Full=Dynactin, 150 kDa isoform;
DE   AltName: Full=150 kDa dynein-associated polypeptide;
DE            Short=DAP-150;
DE            Short=DP-150;
DE   AltName: Full=p150-glued;
GN   Name=ro-3; ORFNames=B8L21.160, NCU03483;
OS   Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS   FGSC 987).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX   NCBI_TaxID=367110;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8744947; DOI=10.1091/mbc.7.5.731;
RA   Tinsley J.H., Minke P.F., Bruno K.S., Plamann M.;
RT   "p150Glued, the largest subunit of the dynactin complex, is nonessential in
RT   Neurospora but required for nuclear distribution.";
RL   Mol. Biol. Cell 7:731-742(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX   PubMed=12655011; DOI=10.1093/nar/gkg293;
RA   Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D.,
RA   Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.;
RT   "What's in the genome of a filamentous fungus? Analysis of the Neurospora
RT   genome sequence.";
RL   Nucleic Acids Res. 31:1944-1954(2003).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX   PubMed=12712197; DOI=10.1038/nature01554;
RA   Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA   Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA   Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA   Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA   Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA   Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA   Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA   Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA   Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA   Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA   DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA   Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA   Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA   Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT   "The genome sequence of the filamentous fungus Neurospora crassa.";
RL   Nature 422:859-868(2003).
CC   -!- FUNCTION: Required for the cytoplasmic dynein-driven retrograde
CC       movement of vesicles and organelles along microtubules. Dynein-dynactin
CC       interaction is a key component of the mechanism of axonal transport of
CC       vesicles and organelles (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Large macromolecular complex of at least 10 components;
CC       p150(glued) binds directly to microtubules and to cytoplasmic dynein.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm. Cytoplasm, cytoskeleton.
CC   -!- SIMILARITY: Belongs to the dynactin 150 kDa subunit family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA80458.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=CAD21101.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; L48661; AAA80458.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AL669989; CAD21101.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CM002237; EAA26534.2; -; Genomic_DNA.
DR   PIR; T18364; T18364.
DR   RefSeq; XP_955770.2; XM_950677.3.
DR   AlphaFoldDB; Q01397; -.
DR   SMR; Q01397; -.
DR   STRING; 5141.EFNCRP00000002615; -.
DR   PRIDE; Q01397; -.
DR   EnsemblFungi; EAA26534; EAA26534; NCU03483.
DR   GeneID; 3871917; -.
DR   KEGG; ncr:NCU03483; -.
DR   VEuPathDB; FungiDB:NCU03483; -.
DR   HOGENOM; CLU_002523_1_1_1; -.
DR   InParanoid; Q01397; -.
DR   OMA; ADKNMRY; -.
DR   Proteomes; UP000001805; Chromosome 6, Linkage Group II.
DR   GO; GO:0051286; C:cell tip; IBA:GO_Central.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0030286; C:dynein complex; IEA:UniProtKB-KW.
DR   GO; GO:0005875; C:microtubule associated complex; IBA:GO_Central.
DR   GO; GO:0035371; C:microtubule plus-end; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0005819; C:spindle; IBA:GO_Central.
DR   GO; GO:0005816; C:spindle pole body; IBA:GO_Central.
DR   GO; GO:0051010; F:microtubule plus-end binding; IBA:GO_Central.
DR   GO; GO:0031122; P:cytoplasmic microtubule organization; IBA:GO_Central.
DR   GO; GO:0000132; P:establishment of mitotic spindle orientation; IBA:GO_Central.
DR   GO; GO:0000743; P:nuclear migration involved in conjugation with cellular fusion; IBA:GO_Central.
DR   Gene3D; 2.30.30.190; -; 1.
DR   InterPro; IPR036859; CAP-Gly_dom_sf.
DR   InterPro; IPR000938; CAP-Gly_domain.
DR   InterPro; IPR022157; Dynactin.
DR   Pfam; PF01302; CAP_GLY; 1.
DR   Pfam; PF12455; Dynactin; 1.
DR   SMART; SM01052; CAP_GLY; 1.
DR   SUPFAM; SSF74924; SSF74924; 1.
DR   PROSITE; PS00845; CAP_GLY_1; 1.
DR   PROSITE; PS50245; CAP_GLY_2; 1.
PE   3: Inferred from homology;
KW   Coiled coil; Cytoplasm; Cytoskeleton; Dynein; Microtubule;
KW   Reference proteome.
FT   CHAIN           1..1367
FT                   /note="Dynactin, 150 kDa isoform"
FT                   /id="PRO_0000083524"
FT   DOMAIN          28..70
FT                   /note="CAP-Gly"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00045"
FT   REGION          76..318
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          321..598
FT                   /evidence="ECO:0000255"
FT   COILED          637..698
FT                   /evidence="ECO:0000255"
FT   COILED          1039..1199
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        76..90
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        91..108
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        132..215
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        226..271
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        302..318
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        1172
FT                   /note="E -> Q (in Ref. 1; AAA80458)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   1367 AA;  152097 MW;  647A99C674131564 CRC64;
     MSELGVAVGQ KIELADGSGR TAFVRYVGET AFAPGTWVGI ELDEPSGKND GSVQGERYFN
     CEMGYGMFVR PTTFNVIAQP PPPPPPSTFR RSVTTRPTSL NASTTRRPAP VDSGLAKRMS
     LNAPSPSPGP RPSRTSSTSI TRSPTRSPTK QLATASSSGN PSRSGTPSTT TKPAGPTTRT
     RPSLSTSRHS MGPPPTPTTR TTRKPSVSSV GTRPSIGATR PVGGRASMSA RSSTNRLSDP
     RESTGSVSSV GKSGFKRGSA SPRSSDEELS ASPVPASPVH QKTAALEKLT APGAGNGGGG
     ASPGATSPNL KATTITPRSS ITNTATMNKE IEDLKAKLKV LEKKRMEDRE KLNSLEKVKA
     ERDKFERIIQ TLQIKYQPQQ QEIQDLKRQL KEAENRLYNV EELQAEHDTA MELATLDREM
     AEETAEVLKV ELDALKQKNE ELELEVEVLR EENSEFTNGM SPEERASTGW LQMERNNERL
     REALIRLRDI TQQQEEELKD QIKSMEEDLR EFETIKEQHT TAKEKLAQTE AIVEDLREQL
     NNALGAEEII ESLTEQTMNQ SEEIKELRAV IDDLESLKEI NDELEINHVQ NEKEMQEEID
     LKDSIIAEQF RQANLQRESL EDMEYTLSRF RELVTSLQSD LEDMRASHAV TENESEQLNS
     RSRAMLDLNM KLQISASKAQ VKTIDLELRR MEAQEAEQHL EIVKLFLPDT YQSDRDSVLA
     LLRFKRLAFK ANLLNGFIKE RVNGQPHPGH EDDIFDGCDA IDKLTWVSAM CDRFVNAISH
     CSLEQFSRYE GALYELEPVE RALNGWIDGL RRDDLKEKQC ADELKRTIAL MTHLGEVHIS
     NDLESFADDV HMKALLMQSH LESAAISVNS LKAMVQRVIP SSGEDDELAQ YFSKRAEAVV
     SQTRSAKVIA GKTVRALEDL KTRSLSLTPD TLEAFEQSET ATRDLANMAR QIGLDLHAFL
     HEEGRTEPYN YMEVQSCIQR SAQASSPTAS SPESDLFNTY LSYLRNATST ISDLASLASD
     LAQTQEFDRT PAPWILRSAE LKAAKTVAPD INDELRRLRD DIAEARRSIA VREEMLSTAQ
     VKIETLESRM RDANAKAARI VDLEADLQAA KKEAAQLQED MEKQDRELKA LESDRDKWKK
     IASESRVVVA DGSGVGVDNK ASAERAVATA REMDALKKEI EALQAAVRYL REDSRRARLK
     EQGDYEWLAE PLVKKKPSVQ EQRKQLVKKE GKAVLGELVK LVSSAKVFDL GSLPEKAEDR
     LKWRPAKTTP GWWVAKQMED WEALKEWEGS VKGRVRELGG VPGSKKAERE EEAKRMVRRT
     AAAKLQIRLP GMEGKVGHGG GGGSGRRVQI VGSREWESLQ GRLAVVV