DYR10_ECOLX
ID DYR10_ECOLX Reviewed; 187 AA.
AC Q04515;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 25-MAY-2022, entry version 99.
DE RecName: Full=Dihydrofolate reductase type A10;
DE EC=1.5.1.3;
DE AltName: Full=Dihydrofolate reductase type X;
DE Short=DHFRX;
GN Name=dfrA10; Synonyms=dfr10;
OS Escherichia coli.
OG Plasmid pDGO100.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=VA292;
RX PubMed=1804022; DOI=10.1128/aac.35.11.2436;
RA Parsons Y., Hall R.M., Stokes H.W.;
RT "A new trimethoprim resistance gene, dhfrX, in the In7 integron of plasmid
RT pDGO100.";
RL Antimicrob. Agents Chemother. 35:2436-2439(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8378445; DOI=10.1006/plas.1993.1032;
RA Stokes H.W., Tomaras C., Parsons Y., Hall R.M.;
RT "The partial 3'-conserved segment duplications in the integrons In6 from
RT pSa and In7 from pDGO100 have a common origin.";
RL Plasmid 30:39-50(1993).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=12499211; DOI=10.1128/aac.47.1.342-349.2003;
RA Partridge S.R., Hall R.M.;
RT "In34, a complex In5 family class 1 integron containing orf513 and
RT dfrA10.";
RL Antimicrob. Agents Chemother. 47:342-349(2003).
CC -!- FUNCTION: Key enzyme in folate metabolism. Catalyzes an essential
CC reaction for de novo glycine and purine synthesis, and for DNA
CC precursor synthesis (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate +
CC H(+) + NADPH; Xref=Rhea:RHEA:15009, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57451, ChEBI:CHEBI:57453, ChEBI:CHEBI:57783,
CC ChEBI:CHEBI:58349; EC=1.5.1.3; Evidence={ECO:0000255|PROSITE-
CC ProRule:PRU00660};
CC -!- PATHWAY: Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-
CC tetrahydrofolate from 7,8-dihydrofolate: step 1/1.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- MISCELLANEOUS: Confers trimethoprim resistance.
CC -!- SIMILARITY: Belongs to the dihydrofolate reductase family.
CC {ECO:0000305}.
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DR EMBL; L06418; AAA92749.1; -; Genomic_DNA.
DR PIR; A49790; A49790.
DR RefSeq; WP_001027119.1; NZ_SHIP01000080.1.
DR AlphaFoldDB; Q04515; -.
DR SMR; Q04515; -.
DR KEGG; ag:AAA92749; -.
DR UniPathway; UPA00077; UER00158.
DR GO; GO:0004146; F:dihydrofolate reductase activity; IEA:UniProtKB-EC.
DR GO; GO:0050661; F:NADP binding; IEA:InterPro.
DR GO; GO:0006545; P:glycine biosynthetic process; IEA:InterPro.
DR GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR GO; GO:0031427; P:response to methotrexate; IEA:UniProtKB-KW.
DR GO; GO:0046654; P:tetrahydrofolate biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd00209; DHFR; 1.
DR Gene3D; 3.40.430.10; -; 1.
DR InterPro; IPR012259; DHFR.
DR InterPro; IPR024072; DHFR-like_dom_sf.
DR InterPro; IPR017925; DHFR_CS.
DR InterPro; IPR001796; DHFR_dom.
DR PANTHER; PTHR48069; PTHR48069; 1.
DR Pfam; PF00186; DHFR_1; 1.
DR SUPFAM; SSF53597; SSF53597; 1.
DR PROSITE; PS00075; DHFR_1; 1.
DR PROSITE; PS51330; DHFR_2; 1.
PE 3: Inferred from homology;
KW Antibiotic resistance; Methotrexate resistance; NADP;
KW One-carbon metabolism; Oxidoreductase; Plasmid; Trimethoprim resistance.
FT CHAIN 1..187
FT /note="Dihydrofolate reductase type A10"
FT /id="PRO_0000186428"
FT DOMAIN 2..174
FT /note="DHFR"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00660"
SQ SEQUENCE 187 AA; 21220 MW; D3E8D81B7AC6E571 CRC64;
MNISLIFANE LITRAFGNQG KLPWQFIKED MQFFQKTTEN SVVVMGLNTW RSLPKMKKLG
RDFIVISSTI TEHEVLNNNI QIFKSFESFL EAFRDTTKPI NVIGGVGLLS EAIEHASTVY
MSSIHMVKPV HADVYVPVEL MNKLYSDFKY PENILWVGDP IDSVYSLSID KFVRPASLVG
VPNDINT