ADIG_MOUSE
ID ADIG_MOUSE Reviewed; 80 AA.
AC Q8R400;
DT 24-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Adipogenin;
DE AltName: Full=Small adipocyte factor 1;
DE Short=SMAF-1;
GN Name=Adig; Synonyms=Smaf1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1] {ECO:0000305, ECO:0000312|EMBL:AAM12949.1}
RP NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND
RP INDUCTION.
RC STRAIN=C57BL/6J {ECO:0000312|EMBL:AAM12949.1};
RX PubMed=15567149; DOI=10.1016/j.bbrc.2004.10.200;
RA Kim J.Y., Tillison K., Smas C.M.;
RT "Cloning, expression, and differentiation-dependent regulation of SMAF1 in
RT adipogenesis.";
RL Biochem. Biophys. Res. Commun. 326:36-44(2005).
RN [2] {ECO:0000305, ECO:0000312|EMBL:ABB18023.1}
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, AND INDUCTION.
RC STRAIN=C57BL/6J {ECO:0000312|EMBL:ABB18023.1};
RX PubMed=16132694; DOI=10.1007/s11010-005-3673-0;
RA Hong Y.-H., Hishikawa D., Miyahara H., Tsuzuki H., Nishimura Y., Gotoh C.,
RA Choi K.-C., Hokari Y., Takagi Y., Lee H.-G., Cho K.-K., Roh S.-G.,
RA Sasaki S.;
RT "Up-regulation of adipogenin, an adipocyte plasma transmembrane protein,
RT during adipogenesis.";
RL Mol. Cell. Biochem. 276:133-141(2005).
RN [3] {ECO:0000312|EMBL:BAE20719.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J {ECO:0000312|EMBL:BAE20719.1};
RC TISSUE=Embryo {ECO:0000312|EMBL:BAE20719.1};
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [5] {ECO:0000312|EMBL:AAH54059.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Mammary gland {ECO:0000312|EMBL:AAH54059.1};
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-63, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brown adipose tissue, and Kidney;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Plays a role in stimulating adipocyte differentiation and
CC development. {ECO:0000269|PubMed:16132694}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Single-pass membrane
CC protein {ECO:0000255}. Nucleus {ECO:0000269|PubMed:15567149,
CC ECO:0000269|PubMed:16132694}.
CC -!- TISSUE SPECIFICITY: Selectively expressed in adipose tissue where it is
CC particularly enriched in brown adipose tissue. In adipose tissue,
CC expressed exclusively in adipocytes and not in the stromal-vascular
CC cell population. Expressed at much lower levels in heart, stomach and
CC muscle and barely detected in kidney and lung.
CC {ECO:0000269|PubMed:15567149, ECO:0000269|PubMed:16132694}.
CC -!- INDUCTION: Induced during adipose conversion of 3T3-L1 cells. Up-
CC regulated in 3T3-L1 adipocytes by troglitazone which stimulates PPARG
CC expression during differentiation. Down-regulated in 3T3-L1 adipocytes
CC exposed to TNF-alpha and to retinoic acid.
CC {ECO:0000269|PubMed:15567149, ECO:0000269|PubMed:16132694}.
CC -!- SIMILARITY: Belongs to the adipogenin family. {ECO:0000305}.
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DR EMBL; AY092026; AAM12949.1; -; mRNA.
DR EMBL; DQ223719; ABB18023.1; -; mRNA.
DR EMBL; AK131610; BAE20719.1; -; mRNA.
DR EMBL; AL663091; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC054059; AAH54059.1; -; mRNA.
DR CCDS; CCDS16990.1; -.
DR RefSeq; NP_663610.1; NM_145635.2.
DR AlphaFoldDB; Q8R400; -.
DR SMR; Q8R400; -.
DR STRING; 10090.ENSMUSP00000051732; -.
DR iPTMnet; Q8R400; -.
DR PhosphoSitePlus; Q8R400; -.
DR jPOST; Q8R400; -.
DR PaxDb; Q8R400; -.
DR PRIDE; Q8R400; -.
DR ProteomicsDB; 296184; -.
DR Antibodypedia; 51583; 12 antibodies from 8 providers.
DR DNASU; 246747; -.
DR Ensembl; ENSMUST00000059889; ENSMUSP00000051732; ENSMUSG00000044405.
DR GeneID; 246747; -.
DR KEGG; mmu:246747; -.
DR UCSC; uc008nqi.1; mouse.
DR CTD; 149685; -.
DR MGI; MGI:2675492; Adig.
DR VEuPathDB; HostDB:ENSMUSG00000044405; -.
DR eggNOG; ENOG502SXJP; Eukaryota.
DR GeneTree; ENSGT00390000018723; -.
DR HOGENOM; CLU_2605436_0_0_1; -.
DR InParanoid; Q8R400; -.
DR OMA; SDLCFNW; -.
DR OrthoDB; 1564414at2759; -.
DR PhylomeDB; Q8R400; -.
DR BioGRID-ORCS; 246747; 3 hits in 72 CRISPR screens.
DR ChiTaRS; C1qtnf7; mouse.
DR PRO; PR:Q8R400; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q8R400; protein.
DR Bgee; ENSMUSG00000044405; Expressed in brown adipose tissue and 98 other tissues.
DR ExpressionAtlas; Q8R400; baseline and differential.
DR Genevisible; Q8R400; MM.
DR GO; GO:0005737; C:cytoplasm; IDA:HGNC-UCL.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005811; C:lipid droplet; IDA:MGI.
DR GO; GO:0005634; C:nucleus; IDA:HGNC-UCL.
DR GO; GO:0050873; P:brown fat cell differentiation; IEP:HGNC-UCL.
DR GO; GO:0045600; P:positive regulation of fat cell differentiation; IMP:HGNC-UCL.
DR GO; GO:0007283; P:spermatogenesis; IEP:BHF-UCL.
DR GO; GO:0050872; P:white fat cell differentiation; IEP:HGNC-UCL.
DR InterPro; IPR027938; Adipogenin.
DR PANTHER; PTHR38499; PTHR38499; 1.
DR Pfam; PF15202; Adipogenin; 1.
PE 1: Evidence at protein level;
KW Membrane; Nucleus; Phosphoprotein; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..80
FT /note="Adipogenin"
FT /id="PRO_0000296376"
FT TRANSMEM 16..36
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MOD_RES 63
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
SQ SEQUENCE 80 AA; 9362 MW; C0DC97A0EF6C37BD CRC64;
MKYPLVPLVS DLTLSFLVFW LCLPVALLLF LTIVWLHFLL SQESKEDDSD LCFNWEPWSK
RPSECGCEET FPGEEDGLHW
