DYR5_ECOLX
ID DYR5_ECOLX Reviewed; 157 AA.
AC P11731;
DT 01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1989, sequence version 1.
DT 25-MAY-2022, entry version 110.
DE RecName: Full=Dihydrofolate reductase type 5;
DE EC=1.5.1.3;
DE AltName: Full=Dihydrofolate reductase type V;
GN Name=dhfrV;
OS Escherichia coli.
OG Plasmid pLMO20.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3054482; DOI=10.1007/bf00339581;
RA Sundstroem L., Radstroem P., Swedberg G., Skoeld O.;
RT "Site-specific recombination promotes linkage between trimethoprim- and
RT sulfonamide resistance genes. Sequence characterization of dhfrV and sulI
RT and a recombination active locus of Tn21.";
RL Mol. Gen. Genet. 213:191-201(1988).
CC -!- FUNCTION: Key enzyme in folate metabolism. Catalyzes an essential
CC reaction for de novo glycine and purine synthesis, and for DNA
CC precursor synthesis (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate +
CC H(+) + NADPH; Xref=Rhea:RHEA:15009, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57451, ChEBI:CHEBI:57453, ChEBI:CHEBI:57783,
CC ChEBI:CHEBI:58349; EC=1.5.1.3; Evidence={ECO:0000255|PROSITE-
CC ProRule:PRU00660};
CC -!- PATHWAY: Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-
CC tetrahydrofolate from 7,8-dihydrofolate: step 1/1.
CC -!- SUBUNIT: Homodimer.
CC -!- MISCELLANEOUS: Confers trimethoprim resistance.
CC -!- SIMILARITY: Belongs to the dihydrofolate reductase family.
CC {ECO:0000305}.
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DR EMBL; X12868; CAA31356.1; -; Genomic_DNA.
DR PIR; S04810; S04810.
DR RefSeq; WP_001317507.1; NZ_VYYW01000155.1.
DR RefSeq; YP_008864016.1; NC_022992.1.
DR RefSeq; YP_008864683.1; NC_022996.1.
DR AlphaFoldDB; P11731; -.
DR SMR; P11731; -.
DR KEGG; ag:CAA31356; -.
DR UniPathway; UPA00077; UER00158.
DR GO; GO:0004146; F:dihydrofolate reductase activity; IEA:UniProtKB-EC.
DR GO; GO:0050661; F:NADP binding; IEA:InterPro.
DR GO; GO:0006545; P:glycine biosynthetic process; IEA:InterPro.
DR GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR GO; GO:0031427; P:response to methotrexate; IEA:UniProtKB-KW.
DR GO; GO:0046654; P:tetrahydrofolate biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd00209; DHFR; 1.
DR Gene3D; 3.40.430.10; -; 1.
DR InterPro; IPR012259; DHFR.
DR InterPro; IPR024072; DHFR-like_dom_sf.
DR InterPro; IPR017925; DHFR_CS.
DR InterPro; IPR001796; DHFR_dom.
DR PANTHER; PTHR48069; PTHR48069; 1.
DR Pfam; PF00186; DHFR_1; 1.
DR SUPFAM; SSF53597; SSF53597; 1.
DR PROSITE; PS00075; DHFR_1; 1.
DR PROSITE; PS51330; DHFR_2; 1.
PE 3: Inferred from homology;
KW Antibiotic resistance; Methotrexate resistance; NADP;
KW One-carbon metabolism; Oxidoreductase; Plasmid; Trimethoprim resistance.
FT CHAIN 1..157
FT /note="Dihydrofolate reductase type 5"
FT /id="PRO_0000186422"
FT DOMAIN 2..156
FT /note="DHFR"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00660"
SQ SEQUENCE 157 AA; 17531 MW; 04B968163A25EFF4 CRC64;
MKVSLMAAKA KNGVIGCGPH IPWSAKGEQL LFKALTYNQW LLVGRKTFES MGALPNRKYA
VVTRSAWTAD NDNVIVFPSI EEAMYGLAEL TDHVIVSGGG EIYRETLPMA STLHISTIDI
EPEGDVFFPN IPNTFEVVFE QHFSSNINYC YQIWQKG