ID   DYR_CAEEL               Reviewed;         189 AA.
AC   Q93341;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   03-AUG-2022, entry version 136.
DE   RecName: Full=Putative dihydrofolate reductase;
DE            EC=1.5.1.3;
GN   Name=dhfr-1; ORFNames=C36B1.7;
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
CC   -!- FUNCTION: Key enzyme in folate metabolism. Catalyzes an essential
CC       reaction for de novo glycine and purine synthesis, and for DNA
CC       precursor synthesis (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate +
CC         H(+) + NADPH; Xref=Rhea:RHEA:15009, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:57451, ChEBI:CHEBI:57453, ChEBI:CHEBI:57783,
CC         ChEBI:CHEBI:58349; EC=1.5.1.3; Evidence={ECO:0000255|PROSITE-
CC         ProRule:PRU00660};
CC   -!- PATHWAY: Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-
CC       tetrahydrofolate from 7,8-dihydrofolate: step 1/1.
CC   -!- SIMILARITY: Belongs to the dihydrofolate reductase family.
CC       {ECO:0000305}.
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DR   EMBL; Z80215; CAB02272.1; -; Genomic_DNA.
DR   PIR; T19778; T19778.
DR   RefSeq; NP_492364.1; NM_059963.3.
DR   AlphaFoldDB; Q93341; -.
DR   SMR; Q93341; -.
DR   BioGRID; 38114; 2.
DR   IntAct; Q93341; 1.
DR   STRING; 6239.C36B1.7; -.
DR   EPD; Q93341; -.
DR   PaxDb; Q93341; -.
DR   PeptideAtlas; Q93341; -.
DR   PRIDE; Q93341; -.
DR   EnsemblMetazoa; C36B1.7.1; C36B1.7.1; WBGene00007974.
DR   GeneID; 172681; -.
DR   KEGG; cel:CELE_C36B1.7; -.
DR   UCSC; C36B1.7; c. elegans.
DR   CTD; 172681; -.
DR   WormBase; C36B1.7; CE05374; WBGene00007974; dhfr-1.
DR   eggNOG; KOG1324; Eukaryota.
DR   GeneTree; ENSGT00940000168797; -.
DR   HOGENOM; CLU_043966_2_3_1; -.
DR   InParanoid; Q93341; -.
DR   OMA; QYEFQMW; -.
DR   OrthoDB; 1489621at2759; -.
DR   PhylomeDB; Q93341; -.
DR   Reactome; R-CEL-196757; Metabolism of folate and pterines.
DR   UniPathway; UPA00077; UER00158.
DR   PRO; PR:Q93341; -.
DR   Proteomes; UP000001940; Chromosome I.
DR   Bgee; WBGene00007974; Expressed in germ line (C elegans) and 4 other tissues.
DR   GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR   GO; GO:0004146; F:dihydrofolate reductase activity; IBA:GO_Central.
DR   GO; GO:0050661; F:NADP binding; IBA:GO_Central.
DR   GO; GO:0046452; P:dihydrofolate metabolic process; IBA:GO_Central.
DR   GO; GO:0046655; P:folic acid metabolic process; IBA:GO_Central.
DR   GO; GO:0006545; P:glycine biosynthetic process; IEA:InterPro.
DR   GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0046654; P:tetrahydrofolate biosynthetic process; IBA:GO_Central.
DR   CDD; cd00209; DHFR; 1.
DR   Gene3D; 3.40.430.10; -; 1.
DR   InterPro; IPR012259; DHFR.
DR   InterPro; IPR024072; DHFR-like_dom_sf.
DR   InterPro; IPR017925; DHFR_CS.
DR   InterPro; IPR001796; DHFR_dom.
DR   PANTHER; PTHR48069; PTHR48069; 1.
DR   Pfam; PF00186; DHFR_1; 1.
DR   SUPFAM; SSF53597; SSF53597; 1.
DR   PROSITE; PS00075; DHFR_1; 1.
DR   PROSITE; PS51330; DHFR_2; 1.
PE   3: Inferred from homology;
KW   NADP; One-carbon metabolism; Oxidoreductase; Reference proteome.
FT   CHAIN           1..189
FT                   /note="Putative dihydrofolate reductase"
FT                   /id="PRO_0000186372"
FT   DOMAIN          3..185
FT                   /note="DHFR"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00660"
FT   BINDING         9
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
FT   BINDING         15..21
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
FT   BINDING         29..34
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         53..55
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
FT   BINDING         69
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         75..77
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
FT   BINDING         115..122
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   189 AA;  21602 MW;  C1642558E097725F CRC64;
     MRKMNLIVAM DAEGGIGKNG VLPWRIKKDM QYFASVTKNV SDQSKRNAVL MGRKCWESIP
     VTRRPLAGRL NIVLSRQLPA QKSDDYIVVN SLEAAMKLLS EPPFVDSIET IWNIGGAEIY
     DLALRENLVD EIHLTRIFKN FEADVHLKSL DFSKMEKVQN AEVSSENSEI FEENGLKFEF
     CKWKVVENH