ADIRF_HUMAN
ID ADIRF_HUMAN Reviewed; 76 AA.
AC Q15847;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 152.
DE RecName: Full=Adipogenesis regulatory factor;
DE AltName: Full=Adipogenesis factor rich in obesity;
DE AltName: Full=Adipose most abundant gene transcript 2 protein;
DE AltName: Full=Adipose-specific protein 2;
DE Short=apM-2;
GN Name=ADIRF; Synonyms=AFRO, APM2, C10orf116;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Adipose tissue;
RA Maeda K.;
RL Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15164054; DOI=10.1038/nature02462;
RA Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L.,
RA Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K.,
RA Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L.,
RA Taylor A., Battles J., Bird C.P., Ainscough R., Almeida J.P.,
RA Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J.,
RA Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y.,
RA Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P.,
RA Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N.,
RA Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A.,
RA Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C.,
RA Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D.,
RA Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C.,
RA Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K.,
RA Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A.,
RA Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S.,
RA McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S.,
RA Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V.,
RA Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A.,
RA Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M.,
RA Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A.,
RA Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P.,
RA Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y.,
RA Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D.,
RA Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.;
RT "The DNA sequence and comparative analysis of human chromosome 10.";
RL Nature 429:375-381(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Pancreas;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP TISSUE SPECIFICITY.
RA Richardson M.;
RL Submitted (SEP-2004) to UniProtKB.
RN [5]
RP FUNCTION IN RESISTANCE TO CISPLATIN, AND INDUCTION.
RX PubMed=19444912; DOI=10.1002/ijc.24465;
RA Scott B.J., Qutob S., Liu Q.Y., Ng C.E.;
RT "APM2 is a novel mediator of cisplatin resistance in a variety of cancer
RT cell types regardless of p53 or MMR status.";
RL Int. J. Cancer 125:1193-1204(2009).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [7]
RP FUNCTION, SUBCELLULAR LOCATION, INDUCTION, AND TISSUE SPECIFICITY.
RX PubMed=23239344; DOI=10.1007/s10863-012-9492-6;
RA Ni Y., Ji C., Wang B., Qiu J., Wang J., Guo X.;
RT "A Novel pro-adipogenesis factor abundant in adipose tissues and over-
RT expressed in obesity acts upstream of PPARgamma and C/EBPalpha.";
RL J. Bioenerg. Biomembr. 45:219-228(2013).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA Ye M., Zou H.;
RT "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT phosphoproteome.";
RL J. Proteomics 96:253-262(2014).
CC -!- FUNCTION: Plays a role in fat cell development; promotes adipogenic
CC differentiation and stimulates transcription initiation of master
CC adipogenesis factors like PPARG and CEBPA at early stages of
CC preadipocyte differentiation. Its overexpression confers resistance to
CC the anticancer chemotherapeutic drug cisplatin.
CC {ECO:0000269|PubMed:19444912, ECO:0000269|PubMed:23239344}.
CC -!- INTERACTION:
CC Q15847; Q14116: IL18; NbExp=3; IntAct=EBI-7162516, EBI-3910835;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:23239344}.
CC -!- TISSUE SPECIFICITY: Expressed in adipose tissue (at protein level).
CC Highly expressed in omental and subcutaneous adipose tissues. Expressed
CC in heart, cornea, liver, kidney and spleen.
CC {ECO:0000269|PubMed:23239344, ECO:0000269|Ref.4}.
CC -!- INDUCTION: Up-regulated during pre-adipocyte differentiation. Up-
CC regulated following DNA damage induced by UV irradiation.
CC {ECO:0000269|PubMed:19444912, ECO:0000269|PubMed:23239344}.
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DR EMBL; D45370; BAA08226.1; -; mRNA.
DR EMBL; AL136982; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC004471; AAH04471.1; -; mRNA.
DR CCDS; CCDS7381.1; -.
DR RefSeq; NP_006820.1; NM_006829.2.
DR AlphaFoldDB; Q15847; -.
DR SMR; Q15847; -.
DR BioGRID; 116171; 3.
DR IntAct; Q15847; 4.
DR MINT; Q15847; -.
DR STRING; 9606.ENSP00000361083; -.
DR GlyGen; Q15847; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; Q15847; -.
DR PhosphoSitePlus; Q15847; -.
DR BioMuta; ADIRF; -.
DR DMDM; 8927989; -.
DR EPD; Q15847; -.
DR jPOST; Q15847; -.
DR MassIVE; Q15847; -.
DR PaxDb; Q15847; -.
DR PeptideAtlas; Q15847; -.
DR PRIDE; Q15847; -.
DR ProteomicsDB; 60790; -.
DR Antibodypedia; 56434; 56 antibodies from 13 providers.
DR DNASU; 10974; -.
DR Ensembl; ENST00000372013.8; ENSP00000361083.3; ENSG00000148671.14.
DR Ensembl; ENST00000561504.1; ENSP00000475582.1; ENSG00000148671.14.
DR GeneID; 10974; -.
DR KEGG; hsa:10974; -.
DR MANE-Select; ENST00000372013.8; ENSP00000361083.3; NM_006829.3; NP_006820.1.
DR CTD; 10974; -.
DR DisGeNET; 10974; -.
DR GeneCards; ADIRF; -.
DR HGNC; HGNC:24043; ADIRF.
DR HPA; ENSG00000148671; Tissue enhanced (adipose).
DR neXtProt; NX_Q15847; -.
DR OpenTargets; ENSG00000148671; -.
DR PharmGKB; PA134930409; -.
DR VEuPathDB; HostDB:ENSG00000148671; -.
DR eggNOG; ENOG502SYWB; Eukaryota.
DR GeneTree; ENSGT00390000017446; -.
DR HOGENOM; CLU_199186_0_0_1; -.
DR InParanoid; Q15847; -.
DR OMA; ETGQKAM; -.
DR OrthoDB; 1637102at2759; -.
DR PhylomeDB; Q15847; -.
DR TreeFam; TF341403; -.
DR PathwayCommons; Q15847; -.
DR Reactome; R-HSA-381340; Transcriptional regulation of white adipocyte differentiation.
DR SignaLink; Q15847; -.
DR BioGRID-ORCS; 10974; 8 hits in 1072 CRISPR screens.
DR ChiTaRS; ADIRF; human.
DR GenomeRNAi; 10974; -.
DR Pharos; Q15847; Tbio.
DR PRO; PR:Q15847; -.
DR Proteomes; UP000005640; Chromosome 10.
DR RNAct; Q15847; protein.
DR Bgee; ENSG00000148671; Expressed in popliteal artery and 199 other tissues.
DR ExpressionAtlas; Q15847; baseline and differential.
DR Genevisible; Q15847; HS.
DR GO; GO:0005829; C:cytosol; IDA:HPA.
DR GO; GO:0070062; C:extracellular exosome; HDA:UniProtKB.
DR GO; GO:0005654; C:nucleoplasm; IDA:HPA.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0045600; P:positive regulation of fat cell differentiation; IDA:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:UniProtKB.
DR InterPro; IPR034450; ADIRF.
DR PANTHER; PTHR39227; PTHR39227; 1.
PE 1: Evidence at protein level;
KW Differentiation; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..76
FT /note="Adipogenesis regulatory factor"
FT /id="PRO_0000064637"
SQ SEQUENCE 76 AA; 7855 MW; C95B41F13C4BA7B1 CRC64;
MASKGLQDLK QQVEGTAQEA VSAAGAAAQQ VVDQATEAGQ KAMDQLAKTT QETIDKTANQ
ASDTFSGIGK KFGLLK
