E13A_SOYBN
ID E13A_SOYBN Reviewed; 347 AA.
AC Q03773;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 25-MAY-2022, entry version 112.
DE RecName: Full=Glucan endo-1,3-beta-glucosidase;
DE EC=3.2.1.39;
DE AltName: Full=(1->3)-beta-glucan endohydrolase;
DE Short=(1->3)-beta-glucanase;
DE AltName: Full=Beta-1,3-endoglucanase;
DE Flags: Precursor;
OS Glycine max (Soybean) (Glycine hispida).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine;
OC Glycine subgen. Soja.
OX NCBI_TaxID=3847;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 186-194 AND 219-224.
RC STRAIN=cv. Harosoy 63; TISSUE=Cotyledon;
RX PubMed=16667522; DOI=10.1104/pp.93.2.673;
RA Takeuchi Y., Yoshikawa M., Takeba G., Tanaka K., Shibata D., Horino O.;
RT "Molecular cloning and ethylene induction of mRNA encoding a phytoalexin
RT elicitor-releasing factor, beta-1,3-endoglucanase, in soybean.";
RL Plant Physiol. 93:673-682(1990).
CC -!- FUNCTION: Is thought to be an important plant defense-related product
CC against fungal pathogens. Is capable of releasing soluble and highly
CC active elicitor molecules from fungus cell walls.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-
CC beta-D-glucans.; EC=3.2.1.39;
CC -!- SUBCELLULAR LOCATION: Vacuole {ECO:0000250}. Note=In intact tissues.
CC {ECO:0000250}.
CC -!- INDUCTION: By ethylene.
CC -!- PTM: The N-terminus is blocked.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 17 family. {ECO:0000305}.
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DR EMBL; M37753; AAA33946.1; -; mRNA.
DR PIR; T07108; T07108.
DR RefSeq; NP_001238474.1; NM_001251545.1.
DR AlphaFoldDB; Q03773; -.
DR SMR; Q03773; -.
DR STRING; 3847.GLYMA03G28850.1; -.
DR CAZy; GH17; Glycoside Hydrolase Family 17.
DR PRIDE; Q03773; -.
DR ProMEX; Q03773; -.
DR EnsemblPlants; KRH66846; KRH66846; GLYMA_03G132700.
DR GeneID; 547822; -.
DR Gramene; KRH66846; KRH66846; GLYMA_03G132700.
DR KEGG; gmx:547822; -.
DR eggNOG; ENOG502QQ3M; Eukaryota.
DR HOGENOM; CLU_024953_0_0_1; -.
DR InParanoid; Q03773; -.
DR OMA; FLVTNRA; -.
DR OrthoDB; 966331at2759; -.
DR Proteomes; UP000008827; Chromosome 3.
DR Genevisible; Q03773; GM.
DR GO; GO:0046658; C:anchored component of plasma membrane; IBA:GO_Central.
DR GO; GO:0005773; C:vacuole; IEA:UniProtKB-SubCell.
DR GO; GO:0042973; F:glucan endo-1,3-beta-D-glucosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR InterPro; IPR000490; Glyco_hydro_17.
DR InterPro; IPR044965; Glyco_hydro_17_plant.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR32227; PTHR32227; 1.
DR Pfam; PF00332; Glyco_hydro_17; 1.
DR SUPFAM; SSF51445; SSF51445; 1.
DR PROSITE; PS00587; GLYCOSYL_HYDROL_F17; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Glycosidase; Hydrolase; Plant defense;
KW Reference proteome; Signal; Vacuole.
FT SIGNAL 1..32
FT /evidence="ECO:0000255"
FT CHAIN 33..347
FT /note="Glucan endo-1,3-beta-glucosidase"
FT /id="PRO_0000011866"
FT ACT_SITE 127
FT /note="Proton donor"
FT /evidence="ECO:0000250|UniProtKB:O22317"
FT ACT_SITE 270
FT /note="Nucleophile"
FT /evidence="ECO:0000250|UniProtKB:O22317"
SQ SEQUENCE 347 AA; 38112 MW; 0FB1B814FDF78753 CRC64;
MAKYHSSGKS SSMTAIAFLF ILLITYTGTT DAQSGVCYGR LGNNLPTPQE VVALYNQANI
RRMRIYGPSP EVLEALRGSN IELLLDIPND NLRNLASSQD NANKWVQDNI KNYANNVRFR
YVSVGNEVKP EHSFAQFLVP ALENIQRAIS NAGLGNQVKV STAIDTGALA ESFPPSKGSF
KSDYRGAYLD GVIRFLVNNN APLMVNVYSY FAYTANPKDI SLDYALFRSP SVVVQDGSLG
YRNLFDASVD AVYAALEKAG GGSLNIVVSE SGWPSSGGTA TSLDNARTYN TNLVRNVKQG
TPKRPGAPLE TYVFAMFDEN QKQPEFEKFW GLFSPITKQP KYSINFN