ID   E13B_SOYBN              Reviewed;         255 AA.
AC   P52395;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   03-AUG-2022, entry version 90.
DE   RecName: Full=Glucan endo-1,3-beta-glucosidase;
DE            EC=3.2.1.39;
DE   AltName: Full=(1->3)-beta-glucan endohydrolase;
DE            Short=(1->3)-beta-glucanase;
DE   AltName: Full=Beta-1,3-endoglucanase;
DE   Flags: Fragment;
OS   Glycine max (Soybean) (Glycine hispida).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine;
OC   Glycine subgen. Soja.
OX   NCBI_TaxID=3847;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Century 84; TISSUE=Leaf;
RX   PubMed=7766051; DOI=10.1007/bf00195722;
RA   Kalinski A., Rowley D.L., Loer D.S., Foley C., Buta G., Herman E.M.;
RT   "Binding-protein expression is subject to temporal, developmental and
RT   stress-induced regulation in terminally differentiated soybean organs.";
RL   Planta 195:611-621(1995).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-
CC         beta-D-glucans.; EC=3.2.1.39;
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 17 family. {ECO:0000305}.
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DR   EMBL; U08405; AAA81955.1; -; mRNA.
DR   PIR; T06359; T06359.
DR   AlphaFoldDB; P52395; -.
DR   SMR; P52395; -.
DR   STRING; 3847.GLYMA12G02410.1; -.
DR   CAZy; GH17; Glycoside Hydrolase Family 17.
DR   PRIDE; P52395; -.
DR   ProMEX; P52395; -.
DR   eggNOG; ENOG502QQ3M; Eukaryota.
DR   Proteomes; UP000008827; Unplaced.
DR   GO; GO:0046658; C:anchored component of plasma membrane; IBA:GO_Central.
DR   GO; GO:0042973; F:glucan endo-1,3-beta-D-glucosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   InterPro; IPR000490; Glyco_hydro_17.
DR   InterPro; IPR044965; Glyco_hydro_17_plant.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR32227; PTHR32227; 1.
DR   Pfam; PF00332; Glyco_hydro_17; 1.
DR   SUPFAM; SSF51445; SSF51445; 1.
DR   PROSITE; PS00587; GLYCOSYL_HYDROL_F17; 1.
PE   2: Evidence at transcript level;
KW   Glycosidase; Hydrolase; Reference proteome.
FT   CHAIN           <1..255
FT                   /note="Glucan endo-1,3-beta-glucosidase"
FT                   /id="PRO_0000205276"
FT   ACT_SITE        48
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250|UniProtKB:O22317"
FT   ACT_SITE        183
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250|UniProtKB:O22317"
FT   NON_TER         1
SQ   SEQUENCE   255 AA;  27671 MW;  12A23BFFF1B3611C CRC64;
     HEIELIMDVA KETLQSLTDS NAATDWVNKY VTPYSQDVNF KYIAVGNEIH PNTNVAQYIL
     SAMTNIQNAI SSRKFTIKVS TAIDSTLITN SYPPNDGVFT SDAEPYIKPI INFLVSNGAP
     LLANVYPYFA YANDQSIPLA YALFTQQGNN DVGYQNLFDA MLDSIYAALE NVGASNLQIV
     VSESGWPSEG GAGASIDNAG TYYANLIRHA SSGDGTPKRP GESIETYLFG RCLSENQKQV
     LILSVIFGLS LPISS