E13B_TOBAC
ID E13B_TOBAC Reviewed; 371 AA.
AC P15797;
DT 01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1994, sequence version 2.
DT 25-MAY-2022, entry version 98.
DE RecName: Full=Glucan endo-1,3-beta-glucosidase, basic vacuolar isoform;
DE EC=3.2.1.39;
DE AltName: Full=(1->3)-beta-glucan endohydrolase;
DE Short=(1->3)-beta-glucanase;
DE AltName: Full=Beta-1,3-endoglucanase, basic;
DE Flags: Precursor;
OS Nicotiana tabacum (Common tobacco).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC Nicotiana.
OX NCBI_TaxID=4097;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, AND PYROGLUTAMATE
RP FORMATION AT GLN-34.
RX PubMed=16593965; DOI=10.1073/pnas.85.15.5541;
RA Shinshi H., Wenzler H., Neuhaus J.-M., Felix G., Hofsteenge J.,
RA Meins F. Jr.;
RT "Evidence for N- and C-terminal processing of a plant defence-related
RT enzyme: the primary structure of tobacco pre-pro-beta-1,3-glucanase.";
RL Proc. Natl. Acad. Sci. U.S.A. 85:5541-5545(1988).
RN [2]
RP PROTEIN SEQUENCE OF 67-91; 106-110; 194-217; 263-291 AND 344-349.
RX PubMed=16594025; DOI=10.1073/pnas.86.8.2673;
RA van den Bulcke M., Bauw G., Castresana C., van Montagu M.,
RA Vandekerckhove J.;
RT "Characterization of vacuolar and extracellular beta(1,3)-glucanases of
RT tobacco: evidence for a strictly compartmentalized plant defense system.";
RL Proc. Natl. Acad. Sci. U.S.A. 86:2673-2677(1989).
CC -!- FUNCTION: Implicated in the defense of plants against pathogens.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-
CC beta-D-glucans.; EC=3.2.1.39;
CC -!- SUBCELLULAR LOCATION: Vacuole.
CC -!- TISSUE SPECIFICITY: Is expressed primarily in epidermal cell of healthy
CC plant, and following induction by ethylene, accumulates in mesophyll
CC cells.
CC -!- INDUCTION: By ethylene.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 17 family. {ECO:0000305}.
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DR EMBL; M20618; AAA34080.1; -; mRNA.
DR EMBL; M20619; AAA34081.1; -; mRNA.
DR EMBL; M20620; AAA34082.1; -; mRNA.
DR PIR; A30758; A30758.
DR AlphaFoldDB; P15797; -.
DR SMR; P15797; -.
DR CAZy; GH17; Glycoside Hydrolase Family 17.
DR Proteomes; UP000084051; Unplaced.
DR GO; GO:0046658; C:anchored component of plasma membrane; IBA:GO_Central.
DR GO; GO:0005773; C:vacuole; IEA:UniProtKB-SubCell.
DR GO; GO:0042973; F:glucan endo-1,3-beta-D-glucosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR InterPro; IPR000490; Glyco_hydro_17.
DR InterPro; IPR044965; Glyco_hydro_17_plant.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR32227; PTHR32227; 1.
DR Pfam; PF00332; Glyco_hydro_17; 1.
DR SUPFAM; SSF51445; SSF51445; 1.
DR PROSITE; PS00587; GLYCOSYL_HYDROL_F17; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Glycoprotein; Glycosidase; Hydrolase;
KW Plant defense; Pyrrolidone carboxylic acid; Reference proteome; Signal;
KW Vacuole.
FT SIGNAL 1..33
FT CHAIN 34..349
FT /note="Glucan endo-1,3-beta-glucosidase, basic vacuolar
FT isoform"
FT /id="PRO_0000011869"
FT PROPEP 350..371
FT /note="Removed in mature form"
FT /id="PRO_0000011870"
FT ACT_SITE 129
FT /note="Proton donor"
FT /evidence="ECO:0000250|UniProtKB:O22317"
FT ACT_SITE 274
FT /note="Nucleophile"
FT /evidence="ECO:0000250|UniProtKB:O22317"
FT MOD_RES 34
FT /note="Pyrrolidone carboxylic acid"
FT /evidence="ECO:0000269|PubMed:16593965"
FT CARBOHYD 362
FT /note="N-linked (GlcNAc...) asparagine"
FT VARIANT 193
FT /note="T -> V"
FT VARIANT 296
FT /note="N -> Y"
FT VARIANT 299
FT /note="Q -> P"
FT VARIANT 346
FT /note="I -> L"
SQ SEQUENCE 371 AA; 40510 MW; 8BB1C273104974C8 CRC64;
MSTSSHKHNT PQMAAITLLG LLLVASSIDI AGAQSIGVCY GMLGNNLPNH WEVIQLYKSR
NIGRLRLYDP NHGALQALKG SNIEVMLGLP NSDVKHIASG MEHARWWVQK NVKDFWPDVK
IKYIAVGNEI SPVTGTSYLT SFLTPAMVNI YKAIGEAGLG NNIKVSTSVD MTLIGNSYPP
SQGSFRNDAR WFTDPIVGFL RDTRAPLLVN IYPYFSYSGN PGQISLPYSL FTAPNVVVQD
GSRQYRNLFD AMLDSVYAAL ERSGGASVGI VVSESGWPSA GAFGATYDNA ATYLRNLIQH
AKEGSPRKPG PIETYIFAMF DENNKNPELE KHFGLFSPNK QPKYNINFGV SGGVWDSSVE
TNATASLVSE M