ID   E13B_WHEAT              Reviewed;         461 AA.
AC   P52409;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   03-AUG-2022, entry version 101.
DE   RecName: Full=Glucan endo-1,3-beta-glucosidase;
DE            EC=3.2.1.39;
DE   AltName: Full=(1->3)-beta-glucan endohydrolase;
DE            Short=(1->3)-beta-glucanase;
DE   AltName: Full=Beta-1,3-endoglucanase;
DE   Flags: Precursor;
GN   Name=GLC1;
OS   Triticum aestivum (Wheat).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX   NCBI_TaxID=4565;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Victory; TISSUE=Root;
RA   Cruz-Ortega R.M., Cushman J.C., Ownby J.D.;
RT   "Nucleotide sequence of cDNA for a 1,3-beta-glucanase associated with
RT   aluminum toxicity in wheat roots.";
RL   (er) Plant Gene Register PGR95-073(1995).
CC   -!- FUNCTION: Is thought to be an important plant defense-related product
CC       against fungal pathogens.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-
CC         beta-D-glucans.; EC=3.2.1.39;
CC   -!- INDUCTION: Associated with aluminum toxicity.
CC   -!- PTM: Contains two additional disulfide bonds. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 17 family. {ECO:0000305}.
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DR   EMBL; U30323; AAA90953.1; -; mRNA.
DR   PIR; T06268; T06268.
DR   AlphaFoldDB; P52409; -.
DR   SMR; P52409; -.
DR   STRING; 4565.Traes_4BS_E8B53F73C.2; -.
DR   CAZy; CBM43; Carbohydrate-Binding Module Family 43.
DR   CAZy; GH17; Glycoside Hydrolase Family 17.
DR   PRIDE; P52409; -.
DR   EnsemblPlants; TraesCAD_scaffold_074949_01G000100.1; TraesCAD_scaffold_074949_01G000100.1; TraesCAD_scaffold_074949_01G000100.
DR   EnsemblPlants; TraesCLE_scaffold_073303_01G000100.1; TraesCLE_scaffold_073303_01G000100.1; TraesCLE_scaffold_073303_01G000100.
DR   EnsemblPlants; TraesCS4A02G175900.1; TraesCS4A02G175900.1; TraesCS4A02G175900.
DR   EnsemblPlants; TraesPAR_scaffold_077265_01G000100.1; TraesPAR_scaffold_077265_01G000100.1; TraesPAR_scaffold_077265_01G000100.
DR   EnsemblPlants; TraesROB_scaffold_073539_01G000200.1; TraesROB_scaffold_073539_01G000200.1; TraesROB_scaffold_073539_01G000200.
DR   EnsemblPlants; TraesWEE_scaffold_083787_01G000100.1; TraesWEE_scaffold_083787_01G000100.1; TraesWEE_scaffold_083787_01G000100.
DR   Gramene; TraesCAD_scaffold_074949_01G000100.1; TraesCAD_scaffold_074949_01G000100.1; TraesCAD_scaffold_074949_01G000100.
DR   Gramene; TraesCLE_scaffold_073303_01G000100.1; TraesCLE_scaffold_073303_01G000100.1; TraesCLE_scaffold_073303_01G000100.
DR   Gramene; TraesCS4A02G175900.1; TraesCS4A02G175900.1; TraesCS4A02G175900.
DR   Gramene; TraesPAR_scaffold_077265_01G000100.1; TraesPAR_scaffold_077265_01G000100.1; TraesPAR_scaffold_077265_01G000100.
DR   Gramene; TraesROB_scaffold_073539_01G000200.1; TraesROB_scaffold_073539_01G000200.1; TraesROB_scaffold_073539_01G000200.
DR   Gramene; TraesWEE_scaffold_083787_01G000100.1; TraesWEE_scaffold_083787_01G000100.1; TraesWEE_scaffold_083787_01G000100.
DR   eggNOG; ENOG502QTAD; Eukaryota.
DR   Proteomes; UP000019116; Unplaced.
DR   ExpressionAtlas; P52409; baseline and differential.
DR   GO; GO:0046658; C:anchored component of plasma membrane; IBA:GO_Central.
DR   GO; GO:0042973; F:glucan endo-1,3-beta-D-glucosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   InterPro; IPR000490; Glyco_hydro_17.
DR   InterPro; IPR044965; Glyco_hydro_17_plant.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR012946; X8.
DR   PANTHER; PTHR32227; PTHR32227; 1.
DR   Pfam; PF00332; Glyco_hydro_17; 1.
DR   Pfam; PF07983; X8; 1.
DR   SMART; SM00768; X8; 1.
DR   SUPFAM; SSF51445; SSF51445; 1.
DR   PROSITE; PS00587; GLYCOSYL_HYDROL_F17; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Glycosidase; Hydrolase; Plant defense; Reference proteome;
KW   Signal.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000250"
FT   CHAIN           24..461
FT                   /note="Glucan endo-1,3-beta-glucosidase"
FT                   /id="PRO_0000011881"
FT   REGION          350..375
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        356..370
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        123
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250|UniProtKB:O22317"
FT   ACT_SITE        265
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250|UniProtKB:O22317"
FT   DISULFID        378..439
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   461 AA;  48873 MW;  7C45F10A026C87F9 CRC64;
     MPLLILLMLL AAGAAGAESA TPSLHIGVNY GANADNLPSP TSVATFLATK TTIDRVKLFD
     ANPTFISAFA GTPISLAVSL PNSALPALAD KATGLDAARS WIRANLSPYV PATNVTLLLA
     GNEILLSTDT NLILSLLPAM RRLAQALKAE GLTGVRVTTP HYLGILAPSD GIPSNASFRA
     GYNTKLFPAM LQFHRDTGSP FMVNPYPYFS YRPETLNYAL FRPNSGIYDP ATKLNYTSML
     DAQMDAIYTA MKKLGYGDVD IAVGEAGWPT QAEPGQIGVG VQEARDFNEG MIRVCSSGKG
     TPLMPNRTFE TYLFSLFDEN QKPGPIAERH FGLFNPDFTP VYDLGLLRDG ASVAPTPSPN
     PSPNPSPKPA PSGGGKWCVA KDGANGTDLQ NNINYACGFV DCKPIQSGGA CFSPNSLQAH
     ASYVMNAYYQ ANGHTDLACD FKGTGIVTSS DPSYGGCKYV S