ID   E13D_TOBAC              Reviewed;         351 AA.
AC   P23433;
DT   01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1991, sequence version 1.
DT   25-MAY-2022, entry version 92.
DE   RecName: Full=Glucan endo-1,3-beta-glucosidase;
DE            EC=3.2.1.39;
DE   AltName: Full=(1->3)-beta-glucan endohydrolase;
DE            Short=(1->3)-beta-glucanase;
DE   AltName: Full=Beta-1,3-endoglucanase;
DE   Flags: Precursor;
GN   Name=SP41B;
OS   Nicotiana tabacum (Common tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4097;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, PYROGLUTAMATE
RP   FORMATION AT GLN-33, GLYCOSYLATION, SUBCELLULAR LOCATION, AND DEVELOPMENTAL
RP   STAGE.
RX   PubMed=2120041; DOI=10.1002/j.1460-2075.1990.tb07550.x;
RA   Ori N., Sessa G., Lotan T., Himmelhoch S., Fluhr R.;
RT   "A major stylar matrix polypeptide (sp41) is a member of the pathogenesis-
RT   related proteins superclass.";
RL   EMBO J. 9:3429-3436(1990).
CC   -!- FUNCTION: Implicated in the defense of plants against pathogens.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-
CC         beta-D-glucans.; EC=3.2.1.39;
CC   -!- SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular
CC       matrix {ECO:0000269|PubMed:2120041}. Note=Stylar secretory matrix.
CC   -!- DEVELOPMENTAL STAGE: Maximal level of accumulation during anthesis.
CC       {ECO:0000269|PubMed:2120041}.
CC   -!- PTM: Glycosylated. {ECO:0000269|PubMed:2120041}.
CC   -!- PTM: The N-terminus is blocked. {ECO:0000269|PubMed:2120041}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 17 family. {ECO:0000305}.
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DR   EMBL; X54431; CAA38303.1; -; mRNA.
DR   PIR; S12014; S12014.
DR   RefSeq; NP_001312176.1; NM_001325247.1.
DR   AlphaFoldDB; P23433; -.
DR   SMR; P23433; -.
DR   CAZy; GH17; Glycoside Hydrolase Family 17.
DR   GeneID; 107777766; -.
DR   KEGG; nta:107777766; -.
DR   Proteomes; UP000084051; Unplaced.
DR   GO; GO:0046658; C:anchored component of plasma membrane; IBA:GO_Central.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR   GO; GO:0042973; F:glucan endo-1,3-beta-D-glucosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   GO; GO:0009626; P:plant-type hypersensitive response; IEA:UniProtKB-KW.
DR   InterPro; IPR000490; Glyco_hydro_17.
DR   InterPro; IPR044965; Glyco_hydro_17_plant.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR32227; PTHR32227; 1.
DR   Pfam; PF00332; Glyco_hydro_17; 1.
DR   SUPFAM; SSF51445; SSF51445; 1.
DR   PROSITE; PS00587; GLYCOSYL_HYDROL_F17; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Extracellular matrix; Glycoprotein; Glycosidase;
KW   Hydrolase; Hypersensitive response; Pathogenesis-related protein;
KW   Plant defense; Pyrrolidone carboxylic acid; Reference proteome; Secreted;
KW   Signal.
FT   SIGNAL          1..32
FT   CHAIN           33..351
FT                   /note="Glucan endo-1,3-beta-glucosidase"
FT                   /id="PRO_0000011876"
FT   ACT_SITE        128
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250|UniProtKB:O22317"
FT   ACT_SITE        268
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250|UniProtKB:O22317"
FT   MOD_RES         33
FT                   /note="Pyrrolidone carboxylic acid"
FT                   /evidence="ECO:0000269|PubMed:2120041"
FT   CARBOHYD        79
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        99
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        235
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   351 AA;  39262 MW;  C13273CFA59A3F8E CRC64;
     MALWYLFNKR SLGAAVLILV GLLMCNIQIT GAQSNIGVCY GEIANNLPSE QDVINLYKAN
     GIRKMRIYYP DTNIFKALNG SNIEIILEVP NQDLEALANS SIANGWVQDN IRSHFPYVKF
     KYISIGNEVS PTNNGQYSQF LLHAMKNVYN ALAAAGLQDK IKVSTATYSG LLANTYPPKD
     SIFREELKSF INPIIEFLAR NNLPLLANIY PYFGHIYNTV DVPLSYALFN QQETNSTGYQ
     NLFDALLDSI YFAVEKAGGP NVEIIVSESG WPSEGNSAAT IENAQTYYRN LVNHVKGGAG
     TPKKPGRIIE TYLFAMFDEN EKQGEITEKH FGLFYPNRAA KYQLNFMYSD S