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E13E_TOBAC
ID   E13E_TOBAC              Reviewed;         370 AA.
AC   P23546; Q9SAT4;
DT   01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1991, sequence version 1.
DT   25-MAY-2022, entry version 101.
DE   RecName: Full=Glucan endo-1,3-beta-glucosidase, basic vacuolar isoform GGIB50;
DE            EC=3.2.1.39;
DE   AltName: Full=(1->3)-beta-glucan endohydrolase;
DE            Short=(1->3)-beta-glucanase;
DE   AltName: Full=Beta-1,3-endoglucanase, basic;
DE   AltName: Full=Glucanase GLA;
DE   Flags: Precursor;
OS   Nicotiana tabacum (Common tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4097;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=cv. Samsun NN;
RX   PubMed=2247445; DOI=10.1073/pnas.87.22.8756;
RA   Linthorst H.J.M., Melchers L.S., Mayer A., van Roekel J.S.C.,
RA   Cornelissen B.J.C., Bol J.F.;
RT   "Analysis of gene families encoding acidic and basic beta-1,3-glucanases of
RT   tobacco.";
RL   Proc. Natl. Acad. Sci. U.S.A. 87:8756-8760(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=cv. Bright Yellow 4;
RX   PubMed=2103484; DOI=10.1007/bf00039434;
RA   Ohme-Takagi M., Shinshi H.;
RT   "Structure and expression of a tobacco beta-1,3-glucanase gene.";
RL   Plant Mol. Biol. 15:941-946(1990).
RN   [3]
RP   NUCLEOTIDE SEQUENCE.
RX   PubMed=2000389; DOI=10.1073/pnas.88.5.1820;
RA   Sperisen C., Ryals J., Meins F.;
RT   "Comparison of cloned genes provides evidence for intergenomic exchange of
RT   DNA in the evolution of a tobacco glucan endo-1,3-beta-glucosidase gene
RT   family.";
RL   Proc. Natl. Acad. Sci. U.S.A. 88:1820-1824(1991).
RN   [4]
RP   NUCLEOTIDE SEQUENCE OF 314-370.
RX   PubMed=2152343; DOI=10.2307/3869130;
RA   Neale A.D., Wahleithner J.A., Lund M., Bonnett H.T., Kelly A.,
RA   Meeks-Wagner D.R., Peacock W.J., Dennis E.S.;
RT   "Chitinase, beta-1,3-glucanase, osmotin, and extensin are expressed in
RT   tobacco explants during flower formation.";
RL   Plant Cell 2:673-684(1990).
RN   [5]
RP   PROTEIN SEQUENCE OF 66-78; 105-109 AND 343-348.
RX   PubMed=1699755; DOI=10.1002/elps.1150110703;
RA   Bauw G., Rasmussen H.H., van den Bulcke M., van Damme J., Puype M.,
RA   Gesser B., Celis J.E., Vandekerckhove J.;
RT   "Two-dimensional gel electrophoresis, protein electroblotting and
RT   microsequencing: a direct link between proteins and genes.";
RL   Electrophoresis 11:528-536(1990).
CC   -!- FUNCTION: Implicated in the defense of plants against pathogens.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-
CC         beta-D-glucans.; EC=3.2.1.39;
CC   -!- SUBCELLULAR LOCATION: Vacuole.
CC   -!- TISSUE SPECIFICITY: Is expressed primarily in epidermal cell of healthy
CC       plant, and following induction by ethylene, accumulates in mesophyll
CC       cells.
CC   -!- INDUCTION: By ethylene and salicylic acid.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 17 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA63541.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; M59442; AAA63541.1; ALT_INIT; Genomic_DNA.
DR   EMBL; X53600; CAA37669.1; -; Genomic_DNA.
DR   EMBL; M60402; AAA63539.1; -; Genomic_DNA.
DR   EMBL; S44870; AAB23376.1; -; mRNA.
DR   PIR; A39115; A39115.
DR   PIR; S12406; S12406.
DR   RefSeq; XP_016505539.1; XM_016650053.1.
DR   AlphaFoldDB; P23546; -.
DR   SMR; P23546; -.
DR   CAZy; GH17; Glycoside Hydrolase Family 17.
DR   GeneID; 107823411; -.
DR   KEGG; nta:107823411; -.
DR   OrthoDB; 966331at2759; -.
DR   Proteomes; UP000084051; Unplaced.
DR   GO; GO:0046658; C:anchored component of plasma membrane; IBA:GO_Central.
DR   GO; GO:0005773; C:vacuole; IEA:UniProtKB-SubCell.
DR   GO; GO:0042973; F:glucan endo-1,3-beta-D-glucosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   InterPro; IPR000490; Glyco_hydro_17.
DR   InterPro; IPR044965; Glyco_hydro_17_plant.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR32227; PTHR32227; 1.
DR   Pfam; PF00332; Glyco_hydro_17; 1.
DR   SUPFAM; SSF51445; SSF51445; 1.
DR   PROSITE; PS00587; GLYCOSYL_HYDROL_F17; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Glycoprotein; Glycosidase; Hydrolase;
KW   Plant defense; Pyrrolidone carboxylic acid; Reference proteome; Signal;
KW   Vacuole.
FT   SIGNAL          1..32
FT   CHAIN           33..348
FT                   /note="Glucan endo-1,3-beta-glucosidase, basic vacuolar
FT                   isoform GGIB50"
FT                   /id="PRO_0000011871"
FT   PROPEP          349..370
FT                   /note="Removed in mature form"
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000011872"
FT   ACT_SITE        128
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250|UniProtKB:O22317"
FT   ACT_SITE        273
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250|UniProtKB:O22317"
FT   MOD_RES         33
FT                   /note="Pyrrolidone carboxylic acid"
FT                   /evidence="ECO:0000250|UniProtKB:P15797"
FT   CARBOHYD        361
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        194
FT                   /note="A -> P (in Ref. 2 and 3)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   370 AA;  40395 MW;  561AE5662478D7A9 CRC64;
     MSTSHKHNTP QMAAITLLGL LLVASSIDIA GAQSIGVCYG MLGNNLPNHW EVIQLYKSRN
     IGRLRLYDPN HGALQALKGS NIEVMLGLPN SDVKHIASGM EHARWWVQKN VKDFWPDVKI
     KYIAVGNEIS PVTGTSYLTS FLTPAMVNIY KAIGEAGLGN NIKVSTSVDM TLIGNSYPPS
     QGSFRNDARW FVDAIVGFLR DTRAPLLVNI YPYFSYSGNP GQISLPYSLF TAPNVVVQDG
     SRQYRNLFDA MLDSVYAALE RSGGASVGIV VSESGWPSAG AFGATYDNAA TYLRNLIQHA
     KEGSPRKPGP IETYIFAMFD ENNKNPELEK HFGLFSPNKQ PKYNINFGVS GGVWDSSVET
     NATASLVSEM
 
 
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