ID   E13F_HORVU              Reviewed;         321 AA.
AC   Q02439;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1994, sequence version 1.
DT   03-AUG-2022, entry version 88.
DE   RecName: Full=Putative glucan endo-1,3-beta-glucosidase GVI;
DE            EC=3.2.1.39;
DE   AltName: Full=(1->3)-beta-glucan endohydrolase GVI;
DE   AltName: Full=(1->3)-beta-glucanase isoenzyme GVI;
DE   AltName: Full=Beta-1,3-endoglucanase GVI;
DE   Flags: Precursor; Fragment;
OS   Hordeum vulgare (Barley).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum.
OX   NCBI_TaxID=4513;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=cv. Clipper, and cv. NK 1558; TISSUE=Seedling;
RX   PubMed=1398132; DOI=10.1016/0378-1119(92)90089-8;
RA   Xu P., Wang J., Fincher G.B.;
RT   "Evolution and differential expression of the (1-->3)-beta-glucan
RT   endohydrolase-encoding gene family in barley, Hordeum vulgare.";
RL   Gene 120:157-165(1992).
CC   -!- FUNCTION: May provide a degree of protection against microbial invasion
CC       of germinated barley grain through its ability to degrade fungal cell
CC       wall polysaccharides.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-
CC         beta-D-glucans.; EC=3.2.1.39;
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 17 family. {ECO:0000305}.
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DR   EMBL; M96941; AAA32957.1; -; Genomic_DNA.
DR   PIR; JC1439; JC1439.
DR   AlphaFoldDB; Q02439; -.
DR   SMR; Q02439; -.
DR   CAZy; GH17; Glycoside Hydrolase Family 17.
DR   ExpressionAtlas; Q02439; differential.
DR   GO; GO:0042973; F:glucan endo-1,3-beta-D-glucosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   InterPro; IPR000490; Glyco_hydro_17.
DR   InterPro; IPR044965; Glyco_hydro_17_plant.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR32227; PTHR32227; 1.
DR   Pfam; PF00332; Glyco_hydro_17; 1.
DR   SUPFAM; SSF51445; SSF51445; 1.
DR   PROSITE; PS00587; GLYCOSYL_HYDROL_F17; 1.
PE   3: Inferred from homology;
KW   Glycosidase; Hydrolase; Plant defense; Signal.
FT   SIGNAL          <1..6
FT                   /evidence="ECO:0000250"
FT   CHAIN           7..321
FT                   /note="Putative glucan endo-1,3-beta-glucosidase GVI"
FT                   /id="PRO_0000011850"
FT   ACT_SITE        100
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250|UniProtKB:O22317"
FT   ACT_SITE        241
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250|UniProtKB:O22317"
FT   NON_TER         1
SQ   SEQUENCE   321 AA;  33344 MW;  D95BC94BF91573D9 CRC64;
     LAGVEGIGVN YGMMGSDLPS PDKVVALYKA NNITDVRIFH PDTNVLEALR NSGLGVVLGT
     LNSDLAPLAS DASYAASWVH SYVQPFAGAV SFRYINAGNE VIPGESAALV LPAMKNLEAA
     LQAAGLSVPV TTAMATSVLG TSYPPSQGTF SEAALPTVGP IVSHLASSGT PLLVNVYPYF
     AYSADPSSVR LDYALLSSSA AVAVTDNGVE YANMFDAILD AVYAAVEKAG GGESLELVVS
     ETGWPSGGGG YGASVENAAA YINNLVRHVG GTPRRPGKAV ETYIFAMFNE NQKPEGVEQN
     FGMFQPDMSQ VYHVDFTASS S