ID   E13F_TOBAC              Reviewed;         370 AA.
AC   P27666;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1992, sequence version 1.
DT   25-MAY-2022, entry version 92.
DE   RecName: Full=Glucan endo-1,3-beta-glucosidase, basic vacuolar isoform GLB;
DE            EC=3.2.1.39;
DE   AltName: Full=(1->3)-beta-glucan endohydrolase;
DE            Short=(1->3)-beta-glucanase;
DE   AltName: Full=Beta-1,3-endoglucanase, basic;
DE   AltName: Full=Glucanase GLB;
DE   Flags: Precursor;
OS   Nicotiana tabacum (Common tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4097;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2000389; DOI=10.1073/pnas.88.5.1820;
RA   Sperisen C., Ryals J., Meins F.;
RT   "Comparison of cloned genes provides evidence for intergenomic exchange of
RT   DNA in the evolution of a tobacco glucan endo-1,3-beta-glucosidase gene
RT   family.";
RL   Proc. Natl. Acad. Sci. U.S.A. 88:1820-1824(1991).
CC   -!- FUNCTION: Implicated in the defense of plants against pathogens.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-
CC         beta-D-glucans.; EC=3.2.1.39;
CC   -!- SUBCELLULAR LOCATION: Vacuole.
CC   -!- TISSUE SPECIFICITY: Is expressed primarily in epidermal cell of healthy
CC       plant, and following induction by ethylene, accumulates in mesophyll
CC       cells.
CC   -!- INDUCTION: By ethylene and salicylic acid.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 17 family. {ECO:0000305}.
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DR   EMBL; M60403; AAA63540.1; -; Genomic_DNA.
DR   PIR; B39115; B39115.
DR   RefSeq; XP_016479922.1; XM_016624436.1.
DR   AlphaFoldDB; P27666; -.
DR   SMR; P27666; -.
DR   CAZy; GH17; Glycoside Hydrolase Family 17.
DR   GeneID; 107801151; -.
DR   KEGG; nta:107801151; -.
DR   OMA; SHNIRRM; -.
DR   OrthoDB; 966331at2759; -.
DR   Proteomes; UP000084051; Unplaced.
DR   GO; GO:0046658; C:anchored component of plasma membrane; IBA:GO_Central.
DR   GO; GO:0005773; C:vacuole; IEA:UniProtKB-SubCell.
DR   GO; GO:0042973; F:glucan endo-1,3-beta-D-glucosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   InterPro; IPR000490; Glyco_hydro_17.
DR   InterPro; IPR044965; Glyco_hydro_17_plant.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR32227; PTHR32227; 1.
DR   Pfam; PF00332; Glyco_hydro_17; 1.
DR   SUPFAM; SSF51445; SSF51445; 1.
DR   PROSITE; PS00587; GLYCOSYL_HYDROL_F17; 1.
PE   2: Evidence at transcript level;
KW   Glycoprotein; Glycosidase; Hydrolase; Plant defense;
KW   Pyrrolidone carboxylic acid; Reference proteome; Signal; Vacuole.
FT   SIGNAL          1..32
FT   CHAIN           33..348
FT                   /note="Glucan endo-1,3-beta-glucosidase, basic vacuolar
FT                   isoform GLB"
FT                   /id="PRO_0000011873"
FT   PROPEP          349..370
FT                   /note="Removed in mature form"
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000011874"
FT   ACT_SITE        128
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250|UniProtKB:O22317"
FT   ACT_SITE        273
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250|UniProtKB:O22317"
FT   MOD_RES         33
FT                   /note="Pyrrolidone carboxylic acid"
FT                   /evidence="ECO:0000250|UniProtKB:P15797"
FT   CARBOHYD        361
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   370 AA;  40443 MW;  3758B156EE9C6AC7 CRC64;
     MSTSDKHNTP QMAAITLLGL LLVASTIEIA GAQSIGVCYG MLGNNLPNHW EVIQLYKSRN
     IGRLRLYDPN HGALQALKGS NIEVMLGLPN SDVKHIASGM EHARWWVQKN VKDFWPDVKI
     KYIAVGNEIS PVTGTSYLTS FLTPAMVNIY KAIGEAGLGN NIKVSTSVDM TLIGNSYPPS
     QGSFRNDARW FTDPIVGFLR DTRAPLLVNI YPYFSYSGNP GQISLPYSLF TAPNVVVQDG
     SRQYRNLFDA MLDSVYAALE RSGGASVGIV VSESGWPSAG AFGATYDNAA TYLRNLIQHA
     KEGSPRKPGP IETYIFAMFD ENNKNPELEK HFGLFSPNKQ PKYNLNFGVS GGVWDSSVET
     NATASLISEM