ID   E13H_TOBAC              Reviewed;         339 AA.
AC   P36401;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1994, sequence version 1.
DT   25-MAY-2022, entry version 103.
DE   RecName: Full=Glucan endo-1,3-beta-glucosidase, acidic isoform PR-Q';
DE            EC=3.2.1.39;
DE   AltName: Full=(1->3)-beta-glucan endohydrolase;
DE            Short=(1->3)-beta-glucanase;
DE   AltName: Full=Beta-1,3-endoglucanase;
DE   AltName: Full=PR-35;
DE   Flags: Precursor;
OS   Nicotiana tabacum (Common tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4097;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE.
RC   STRAIN=cv. Xanthi;
RX   PubMed=2103473; DOI=10.1007/bf00039420;
RA   Payne G., Ward E., Gaffney T., Ahl Goy P., Moyer M., Harper A.,
RA   Meins F. Jr., Ryals J.;
RT   "Evidence for a third structural class of beta-1,3-glucanase in tobacco.";
RL   Plant Mol. Biol. 15:797-808(1990).
RN   [2]
RP   PROTEIN SEQUENCE OF 57-69; 112-126; 281-291; 297-321 AND 333-339.
RX   PubMed=16594025; DOI=10.1073/pnas.86.8.2673;
RA   van den Bulcke M., Bauw G., Castresana C., van Montagu M.,
RA   Vandekerckhove J.;
RT   "Characterization of vacuolar and extracellular beta(1,3)-glucanases of
RT   tobacco: evidence for a strictly compartmentalized plant defense system.";
RL   Proc. Natl. Acad. Sci. U.S.A. 86:2673-2677(1989).
CC   -!- FUNCTION: Implicated in the defense of plants against pathogens.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-
CC         beta-D-glucans.; EC=3.2.1.39;
CC   -!- SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast.
CC   -!- INDUCTION: Accumulates following infection.
CC   -!- PTM: The N-terminus is blocked.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 17 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA38324.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; X54456; CAA38324.1; ALT_INIT; mRNA.
DR   PIR; S12402; S12402.
DR   RefSeq; XP_016495818.1; XM_016640332.1.
DR   AlphaFoldDB; P36401; -.
DR   SMR; P36401; -.
DR   CAZy; GH17; Glycoside Hydrolase Family 17.
DR   GeneID; 107814850; -.
DR   KEGG; nta:107814850; -.
DR   OMA; FLVTNRA; -.
DR   OrthoDB; 966331at2759; -.
DR   PhylomeDB; P36401; -.
DR   Proteomes; UP000084051; Unplaced.
DR   GO; GO:0046658; C:anchored component of plasma membrane; IBA:GO_Central.
DR   GO; GO:0048046; C:apoplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0042973; F:glucan endo-1,3-beta-D-glucosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   InterPro; IPR000490; Glyco_hydro_17.
DR   InterPro; IPR044965; Glyco_hydro_17_plant.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR32227; PTHR32227; 1.
DR   Pfam; PF00332; Glyco_hydro_17; 1.
DR   SUPFAM; SSF51445; SSF51445; 1.
DR   PROSITE; PS00587; GLYCOSYL_HYDROL_F17; 1.
PE   1: Evidence at protein level;
KW   Apoplast; Direct protein sequencing; Glycosidase; Hydrolase; Plant defense;
KW   Pyrrolidone carboxylic acid; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   CHAIN           25..339
FT                   /note="Glucan endo-1,3-beta-glucosidase, acidic isoform PR-
FT                   Q'"
FT                   /id="PRO_0000011878"
FT   ACT_SITE        118
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250|UniProtKB:O22317"
FT   ACT_SITE        262
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250|UniProtKB:O22317"
FT   MOD_RES         25
FT                   /note="Pyrrolidone carboxylic acid"
FT                   /evidence="ECO:0000250|UniProtKB:P15797"
FT   CONFLICT        297
FT                   /note="R -> A (in Ref. 2; AA sequence)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   339 AA;  36995 MW;  FDFA0D4F7300235E CRC64;
     MAHLIVTLLL LSVLTLATLD FTGAQAGVCY GRQGNGLPSP ADVVSLCNRN NIRRMRIYDP
     DQPTLEALRG SNIELMLGVP NPDLENVAAS QANADTWVQN NVRNYGNVKF RYIAVGNEVS
     PLNENSKYVP VLLNAMRNIQ TAISGAGLGN QIKVSTAIET GLTTDTSPPS NGRFKDDVRQ
     FIEPIINFLV TNRAPLLVNL YPYFAIANNA DIKLEYALFT SSEVVVNDNG RGYRNLFDAI
     LDATYSALEK ASGSSLEIVV SESGWPSAGA GQLTSIDNAR TYNNNLISHV KGGSPKRPSG
     PIETYVFALF DEDQKDPEIE KHFGLFSANM QPKYQISFN