ID   E13L_TOBAC              Reviewed;         356 AA.
AC   P52399;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=Glucan endo-1,3-beta-glucosidase, acidic isoform GL153;
DE            EC=3.2.1.39;
DE   AltName: Full=(1->3)-beta-glucan endohydrolase;
DE            Short=(1->3)-beta-glucanase;
DE   AltName: Full=Beta-1,3-endoglucanase;
DE   Flags: Precursor;
GN   Name=GGL4;
OS   Nicotiana tabacum (Common tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4097;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=cv. Xanthi NC; TISSUE=Leaf;
RX   PubMed=16668198; DOI=10.1104/pp.96.2.390;
RA   Ward E.R., Payne G.B., Moyer M.B., Williams S.C., Dincher S.S.,
RA   Sharkey K.C., Beck J.J., Taylor H.T., Ahl-Goy P., Meins F., Ryals J.A.;
RT   "Differential regulation of beta-1,3-glucanase messenger RNAs in response
RT   to pathogen infection.";
RL   Plant Physiol. 96:390-397(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=cv. Xanthi NC;
RA   Chen H.-J., Hou W.-C., Lin Y.-H.;
RT   "Isolation of a genomic DNA clone (GGL4) encoding a beta-1,3-glucanase from
RT   Nicotiana tabacum cv. Xanthi nc.";
RL   Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Is thought to be an important plant defense-related product
CC       against fungal pathogens.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-
CC         beta-D-glucans.; EC=3.2.1.39;
CC   -!- SUBCELLULAR LOCATION: Secreted, extracellular space.
CC   -!- TISSUE SPECIFICITY: Is expressed primarily in epidermal cell of healthy
CC       plant, and following induction by ethylene, accumulates in mesophyll
CC       cells.
CC   -!- INDUCTION: By viral infection.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 17 family. {ECO:0000305}.
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DR   EMBL; M60463; AAA34079.1; -; mRNA.
DR   EMBL; AF141654; AAD33881.1; -; Genomic_DNA.
DR   PIR; T03249; T03249.
DR   RefSeq; NP_001312401.1; NM_001325472.1.
DR   AlphaFoldDB; P52399; -.
DR   SMR; P52399; -.
DR   CAZy; GH17; Glycoside Hydrolase Family 17.
DR   GeneID; 107789548; -.
DR   KEGG; nta:107789548; -.
DR   OMA; FVHISNT; -.
DR   PhylomeDB; P52399; -.
DR   Proteomes; UP000084051; Unplaced.
DR   GO; GO:0046658; C:anchored component of plasma membrane; IBA:GO_Central.
DR   GO; GO:0005615; C:extracellular space; IEA:UniProtKB-SubCell.
DR   GO; GO:0042973; F:glucan endo-1,3-beta-D-glucosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   InterPro; IPR000490; Glyco_hydro_17.
DR   InterPro; IPR044965; Glyco_hydro_17_plant.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR32227; PTHR32227; 1.
DR   Pfam; PF00332; Glyco_hydro_17; 1.
DR   SUPFAM; SSF51445; SSF51445; 1.
DR   PROSITE; PS00587; GLYCOSYL_HYDROL_F17; 1.
PE   2: Evidence at transcript level;
KW   Glycoprotein; Glycosidase; Hydrolase; Plant defense;
KW   Pyrrolidone carboxylic acid; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..29
FT                   /evidence="ECO:0000255"
FT   CHAIN           30..356
FT                   /note="Glucan endo-1,3-beta-glucosidase, acidic isoform
FT                   GL153"
FT                   /id="PRO_0000011880"
FT   ACT_SITE        124
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250|UniProtKB:O22317"
FT   ACT_SITE        264
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250|UniProtKB:O22317"
FT   MOD_RES         30
FT                   /note="Pyrrolidone carboxylic acid"
FT                   /evidence="ECO:0000250|UniProtKB:P15797"
FT   CARBOHYD        95
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   356 AA;  39489 MW;  640A904884C4A4DE CRC64;
     MALCIKNGFL AAALVLVGLL MCSIQMIGAQ SIGVCYGKIA NNLPSEQDVI NLYKANGIRK
     MRIYYPDKNI FKALKGSNIE IILDVPNQDL EALANSSIAN GWVQDNIRSH FPYVKFKYIS
     IGNEVSPINN GQYSQFLLHA MENVYNALAA SGLQDKIKVT TATYSGLLAN TYPPKASIFR
     GEFNSFINPI IQFLAQNNLP LLANVYPYFV HISNTADVPL SYALFTQRGK NSAGYQNLFD
     AILDSMYFAV EKAGGPNVEI IVSESGWPSE GNSAATIENA QTYYRNLIDH VKRGAGTPKK
     PGKSIETYLF AMFDENVKKG EITEKHFGLF SPDQRAKYQL NFNSLMPIYI DISRVI