ID   E1A_ADEM1               Reviewed;         200 AA.
AC   P12534;
DT   01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1989, sequence version 1.
DT   23-FEB-2022, entry version 64.
DE   RecName: Full=Early E1A 21 kDa protein;
OS   Murine adenovirus A serotype 1 (MAdV-1) (Murine adenovirus 1).
OC   Viruses; Varidnaviria; Bamfordvirae; Preplasmiviricota; Tectiliviricetes;
OC   Rowavirales; Adenoviridae; Mastadenovirus; Murine mastadenovirus A.
OX   NCBI_TaxID=10530;
OH   NCBI_TaxID=10090; Mus musculus (Mouse).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3172335; DOI=10.1128/jvi.62.11.3947-3957.1988;
RA   Ball A.O., Williams M.E., Spindler K.R.;
RT   "Identification of mouse adenovirus type 1 early region 1: DNA sequence and
RT   a conserved transactivating function.";
RL   J. Virol. 62:3947-3957(1988).
CC   -!- FUNCTION: E1A protein has both transforming and trans-activating
CC       activities. Plays a role in viral genome replication by driving entry
CC       of quiescent cells into the cell cycle. Disrupts the function of host
CC       retinoblastoma protein RB1/pRb and isoform early E1A 26 kDa protein
CC       stabilizes TP53, which are key regulators of the cell cycle. Induces
CC       the disassembly of the E2F1 transcription factors from RB1 by direct
CC       competition for the same binding site on RB1, with subsequent
CC       transcriptional activation of E2F1-regulated S-phase genes.
CC       Inactivation of the ability of RB1 to arrest the cell cycle is critical
CC       for cellular transformation, uncontrolled cellular growth and
CC       proliferation induced by viral infection. Stimulation of progression
CC       from G1 to S phase allows the virus to efficiently use the cellular DNA
CC       replicating machinery to achieve viral genome replication (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Interaction with host RB1 induces the aberrant dissociation of
CC       RB1-E2F1 complex thereby disrupting RB1's activity. {ECO:0000250}.
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DR   EMBL; M22245; AAA42424.1; -; Genomic_DNA.
DR   RefSeq; NP_015532.1; NC_000942.1.
DR   IntAct; P12534; 2.
DR   GeneID; 1732772; -.
DR   KEGG; vg:1732772; -.
DR   Proteomes; UP000243112; Genome.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0039645; P:modulation by virus of host G1/S transition checkpoint; IEA:UniProtKB-KW.
DR   GO; GO:0039563; P:suppression by virus of host JAK-STAT cascade via inhibition of STAT1 activity; IEA:UniProtKB-KW.
DR   GO; GO:0039502; P:suppression by virus of host type I interferon-mediated signaling pathway; IEA:UniProtKB-KW.
PE   3: Inferred from homology;
KW   Activator; Early protein;
KW   G1/S host cell cycle checkpoint dysregulation by virus;
KW   Host-virus interaction; Inhibition of host innate immune response by virus;
KW   Inhibition of host interferon signaling pathway by virus;
KW   Inhibition of host STAT1 by virus; Metal-binding;
KW   Modulation of host cell cycle by virus; Transcription;
KW   Transcription regulation; Viral immunoevasion; Zinc; Zinc-finger.
FT   CHAIN           1..200
FT                   /note="Early E1A 21 kDa protein"
FT                   /id="PRO_0000221704"
FT   ZN_FING         136..151
FT                   /evidence="ECO:0000255"
FT   REGION          78..98
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           114..118
FT                   /note="LXCXE motif, interaction with host RB1"
FT                   /evidence="ECO:0000255"
FT   MOTIF           196..200
FT                   /note="Nuclear localization signal"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   200 AA;  21878 MW;  5B63D6E6272639E5 CRC64;
     MSRLLRLSLS SRVWLAAQEA TRNVSEDPVV CRTPWDGSPT CTAVRVVRAE VLADGTMDLD
     IVFPEAAVQA VFSRTPWQDS TTATSAEEPS ASTDSISSDP LPISCVESFE DMDLRCYEQL
     SPSPESIETI EVFPPCSTCG GHEVNGFCSL CYLRGLTDLL PQADDAGEAE VPDESAKDLC
     FMDLLTWAME DKTECSRHDE