E1BS_ADE05
ID E1BS_ADE05 Reviewed; 176 AA.
AC P03246;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 21-JUL-1986, sequence version 1.
DT 23-FEB-2022, entry version 109.
DE RecName: Full=E1B protein, small T-antigen;
DE AltName: Full=E1B 19 kDa protein;
DE Short=E1B-19K;
OS Human adenovirus C serotype 5 (HAdV-5) (Human adenovirus 5).
OC Viruses; Varidnaviria; Bamfordvirae; Preplasmiviricota; Tectiliviricetes;
OC Rowavirales; Adenoviridae; Mastadenovirus.
OX NCBI_TaxID=28285;
OH NCBI_TaxID=9606; Homo sapiens (Human).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=7326748; DOI=10.1016/0092-8674(81)90366-4;
RA Bos J.L., Polder L.J., Bernards R., Schrier P.I., van den Elsen P.J.,
RA van der Eb A.J., van Ormondt H.;
RT "The 2.2 kb E1b mRNA of human Ad12 and Ad5 codes for two tumor antigens
RT starting at different AUG triplets.";
RL Cell 27:121-131(1981).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=6260576; DOI=10.1016/0378-1119(80)90070-0;
RA van Ormondt H., Maat J., van Beveren C.P.;
RT "The nucleotide sequence of the transforming early region E1 of adenovirus
RT type 5 DNA.";
RL Gene 11:299-309(1980).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=1727603; DOI=10.1016/0042-6822(92)90082-z;
RA Chroboczek J., Bieber F., Jacrot B.;
RT "The sequence of the genome of adenovirus type 5 and its comparison with
RT the genome of adenovirus type 2.";
RL Virology 186:280-285(1992).
RN [4]
RP MUTAGENESIS, AND INTERACTION WITH BAX.
RX PubMed=8600029; DOI=10.1101/gad.10.4.461;
RA Han J., Sabbatini P., Perez D., Rao L., Modha D., White E.;
RT "The E1B 19K protein blocks apoptosis by interacting with and inhibiting
RT the p53-inducible and death-promoting Bax protein.";
RL Genes Dev. 10:461-477(1996).
RN [5]
RP ALTERNATIVE SPLICING (ISOFORMS E1B-496R; E1B-176R; E1B-156R; E1B-93R AND
RP E1B-84R).
RX PubMed=551290; DOI=10.1038/281694a0;
RA Perricaudet M., Akusjaervi G., Virtanen A., Pettersson U.;
RT "Structure of two spliced mRNAs from the transforming region of human
RT subgroup C adenoviruses.";
RL Nature 281:694-696(1979).
RN [6]
RP ALTERNATIVE SPLICING (ISOFORMS E1B-496R; E1B-176R; E1B-156R; E1B-93R AND
RP E1B-84R).
RX PubMed=8151295; DOI=10.1099/0022-1317-75-4-789;
RA Takayesu D., Teodoro J.G., Whalen S.G., Branton P.E.;
RT "Characterization of the 55K adenovirus type 5 E1B product and related
RT proteins.";
RL J. Gen. Virol. 75:789-798(1994).
CC -!- FUNCTION: Putative adenovirus Bcl-2 homolog that inhibits apoptosis
CC induced by TNF or FAS pathways, as well as p53-mediated apoptosis.
CC Without E1B 19K function, virus production is compromised because of
CC premature death of host cell. Interacts with Bax protein in cell
CC lysates.
CC -!- SUBCELLULAR LOCATION: Host cell membrane. Host nucleus envelope. Host
CC nucleus lamina. Note=Associated with the plasma and nuclear membranes,
CC and with the insoluble nuclear lamina.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=5;
CC Comment=At least five different polypeptides are generated by
CC alternative splicing of a common mRNA precursor.;
CC Name=E1B-176R; Synonyms=E1B-19K;
CC IsoId=P03246-1; Sequence=Displayed;
CC Name=E1B-496R; Synonyms=E1B-55K;
CC IsoId=P03243-1; Sequence=External;
CC Name=E1B-156R; Synonyms=E1B-18K;
CC IsoId=P03243-2; Sequence=External;
CC Name=E1B-93R; Synonyms=E1B-16K;
CC IsoId=P03243-3; Sequence=External;
CC Name=E1B-84R; Synonyms=E1B-15K;
CC IsoId=P03243-4; Sequence=External;
CC -!- SIMILARITY: Belongs to the adenoviridae E1B 19 kDa protein family.
CC {ECO:0000305}.
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DR EMBL; M73260; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; X02996; CAA26741.1; -; Genomic_DNA.
DR PIR; A03814; Q1AD25.
DR RefSeq; AP_000198.1; AC_000008.1. [P03246-1]
DR IntAct; P03246; 95.
DR MINT; P03246; -.
DR Proteomes; UP000004992; Genome.
DR GO; GO:0044203; C:host cell nuclear lamina; IEA:UniProtKB-SubCell.
DR GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0019050; P:suppression by virus of host apoptotic process; IEA:UniProtKB-KW.
DR InterPro; IPR002924; Adenovir_t-Ag_E1B_19kDa.
DR InterPro; IPR002475; Bcl2-like.
DR Pfam; PF01691; Adeno_E1B_19K; 1.
DR PROSITE; PS50062; BCL2_FAMILY; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Apoptosis; Early protein; Host cell membrane;
KW Host membrane; Host nucleus; Host-virus interaction;
KW Inhibition of host apoptosis by viral BCL2-like protein; Membrane;
KW Modulation of host cell apoptosis by virus.
FT CHAIN 1..176
FT /note="E1B protein, small T-antigen"
FT /id="PRO_0000221712"
FT REGION 146..176
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 51
FT /note="F->S: No interaction with BAX and loss of anti-
FT apoptotic activity."
FT /evidence="ECO:0000269|PubMed:8600029"
FT MUTAGEN 87
FT /note="G->A: No interaction with BAX and loss of anti-
FT apoptotic activity."
FT /evidence="ECO:0000269|PubMed:8600029"
SQ SEQUENCE 176 AA; 20614 MW; 5ECBDA61978C29CB CRC64;
MEAWECLEDF SAVRNLLEQS SNSTSWFWRF LWGSSQAKLV CRIKEDYKWE FEELLKSCGE
LFDSLNLGHQ ALFQEKVIKT LDFSTPGRAA AAVAFLSFIK DKWSEETHLS GGYLLDFLAM
HLWRAVVRHK NRLLLLSSVR PAIIPTEEQQ QQQEEARRRR QEQSPWNPRA GLDPRE
