ID   E248R_ASFB7             Reviewed;         248 AA.
AC   Q65200;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 66.
DE   RecName: Full=Inner membrane protein pE248R;
DE            Short=pE248R;
GN   OrderedLocusNames=Ba71V-132; ORFNames=E248R;
OS   African swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V)
OS   (ASFV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Asfuvirales; Asfarviridae; Asfivirus.
OX   NCBI_TaxID=10498;
OH   NCBI_TaxID=6937; Ornithodoros (relapsing fever ticks).
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=11831707; DOI=10.1006/viro.1995.1149;
RA   Yanez R.J., Rodriguez J.M., Nogal M.L., Yuste L., Enriquez C.,
RA   Rodriguez J.F., Vinuela E.;
RT   "Analysis of the complete nucleotide sequence of African swine fever
RT   virus.";
RL   Virology 208:249-278(1995).
RN   [2]
RP   INTERACTION WITH A151R.
RX   PubMed=16537584; DOI=10.1128/jvi.80.7.3157-3166.2006;
RA   Rodriguez I., Redrejo-Rodriguez M., Rodriguez J.M., Alejo A., Salas J.,
RA   Salas M.L.;
RT   "African swine fever virus pB119L protein is a flavin adenine dinucleotide-
RT   linked sulfhydryl oxidase.";
RL   J. Virol. 80:3157-3166(2006).
RN   [3]
RP   SUBCELLULAR LOCATION, FUNCTION, TOPOLOGY, DISULFIDE BOND, AND
RP   MYRISTOYLATION AT GLY-2.
RX   PubMed=19793823; DOI=10.1128/jvi.01333-09;
RA   Rodriguez I., Nogal M.L., Redrejo-Rodriguez M., Bustos M.J., Salas M.L.;
RT   "The African swine fever virus virion membrane protein pE248R is required
RT   for virus infectivity and an early postentry event.";
RL   J. Virol. 83:12290-12300(2009).
RN   [4]
RP   SUBCELLULAR LOCATION.
RX   PubMed=30185597; DOI=10.1128/jvi.01293-18;
RA   Alejo A., Matamoros T., Guerra M., Andres G.;
RT   "A Proteomic Atlas of the African Swine Fever Virus Particle.";
RL   J. Virol. 92:0-0(2018).
CC   -!- FUNCTION: Essential for viral fusion with host endosomal membrane and
CC       core release. {ECO:0000269|PubMed:19793823}.
CC   -!- SUBUNIT: Interacts with A151R. {ECO:0000269|PubMed:16537584}.
CC   -!- SUBCELLULAR LOCATION: Host membrane {ECO:0000269|PubMed:19793823};
CC       Single-pass type II membrane protein {ECO:0000269|PubMed:19793823}.
CC       Virion membrane {ECO:0000305|PubMed:19793823,
CC       ECO:0000305|PubMed:30185597}. Note=Probably part of the virion inner
CC       membrane. {ECO:0000269|PubMed:19793823}.
CC   -!- SIMILARITY: Belongs to the asfivirus E248R family. {ECO:0000305}.
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DR   EMBL; U18466; AAA65360.1; -; Genomic_DNA.
DR   RefSeq; NP_042824.1; NC_001659.2.
DR   SMR; Q65200; -.
DR   TCDB; 1.G.23.1.1; african swine fever virus (asfv) fusion protein pe199l (asfv-fp) family.
DR   iPTMnet; Q65200; -.
DR   PRIDE; Q65200; -.
DR   GeneID; 22220360; -.
DR   KEGG; vg:22220360; -.
DR   Proteomes; UP000000624; Genome.
DR   GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0039641; C:viral inner membrane; IEA:UniProtKB-KW.
DR   GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0019064; P:fusion of virus membrane with host plasma membrane; IEA:UniProtKB-KW.
DR   GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR   InterPro; IPR003472; Virion_mem_poxvirus_L1.
DR   Pfam; PF02442; L1R_F9L; 1.
PE   1: Evidence at protein level;
KW   Capsid inner membrane protein;
KW   Fusion of virus membrane with host cell membrane;
KW   Fusion of virus membrane with host membrane; Host membrane; Lipoprotein;
KW   Membrane; Myristate; Reference proteome; Signal-anchor; Transmembrane;
KW   Transmembrane helix; Viral penetration into host cytoplasm; Virion;
KW   Virus entry into host cell.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000255"
FT   CHAIN           2..248
FT                   /note="Inner membrane protein pE248R"
FT                   /id="PRO_0000373604"
FT   TOPO_DOM        2..199
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000269|PubMed:19793823"
FT   TRANSMEM        200..220
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        221..248
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000269|PubMed:19793823"
FT   LIPID           2
FT                   /note="N-myristoyl glycine; by host"
FT                   /evidence="ECO:0000269|PubMed:19793823"
SQ   SEQUENCE   248 AA;  27462 MW;  F5876DA30FAE21E5 CRC64;
     MGGSTSKNSF KNTTNIISNS IFNQMQNCIS MLDGKNYIGV FGDGNILNHV FQDLNLSLDT
     SCVQKHVNEE NFITNLSNQI TQNLKDQEVA LTQWMDAGHH DQKTDIEENI KVNLTTTLIQ
     NCVSSLSGMN VLVVKGNGNI VENATQKQSQ QIISNCLQGS KQAIDTTTGI TNTVNQYSHY
     TSKNFFDFIA DAISAVFKNI MVAAVVIVLI IVGFIAVFYF LHSRHRHEEE EEAEPLISNK
     VLKNAAVS