ID   E248R_ASFM2             Reviewed;         236 AA.
AC   Q65244;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 56.
DE   RecName: Full=Inner membrane protein pE248R;
DE            Short=pE248R;
GN   OrderedLocusNames=Mal-140; ORFNames=k2R;
OS   African swine fever virus (isolate Tick/Malawi/Lil 20-1/1983) (ASFV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Asfuvirales; Asfarviridae; Asfivirus.
OX   NCBI_TaxID=10500;
OH   NCBI_TaxID=6937; Ornithodoros (relapsing fever ticks).
OH   NCBI_TaxID=85517; Phacochoerus aethiopicus (Warthog).
OH   NCBI_TaxID=41426; Phacochoerus africanus (Warthog).
OH   NCBI_TaxID=273792; Potamochoerus larvatus (Bushpig).
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8021596; DOI=10.1099/0022-1317-75-7-1655;
RA   Dixon L.K., Twigg S.R.F., Baylis S.A., Vydelingum S., Bristow C.,
RA   Hammond J.M., Smith G.L.;
RT   "Nucleotide sequence of a 55 kbp region from the right end of the genome of
RT   a pathogenic African swine fever virus isolate (Malawi LIL20/1).";
RL   J. Gen. Virol. 75:1655-1684(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Kutish G.F., Rock D.L.;
RT   "African swine fever virus genomes.";
RL   Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Essential for viral fusion with host endosomal membrane and
CC       core release. {ECO:0000250|UniProtKB:Q65200}.
CC   -!- SUBUNIT: Interacts with A151R. {ECO:0000250|UniProtKB:Q65200}.
CC   -!- SUBCELLULAR LOCATION: Host membrane {ECO:0000250|UniProtKB:Q65200};
CC       Single-pass type II membrane protein {ECO:0000250|UniProtKB:Q65200}.
CC       Virion membrane {ECO:0000250|UniProtKB:Q65200}. Note=Probably part of
CC       the virion inner membrane. {ECO:0000250|UniProtKB:Q65200}.
CC   -!- SIMILARITY: Belongs to the asfivirus E248R family. {ECO:0000305}.
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DR   EMBL; X71982; CAA50840.1; -; Genomic_DNA.
DR   EMBL; AY261361; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   SMR; Q65244; -.
DR   Proteomes; UP000000860; Genome.
DR   GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0039641; C:viral inner membrane; IEA:UniProtKB-KW.
DR   GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR003472; Virion_mem_poxvirus_L1.
DR   Pfam; PF02442; L1R_F9L; 1.
PE   3: Inferred from homology;
KW   Capsid inner membrane protein; Host membrane; Lipoprotein; Membrane;
KW   Myristate; Signal-anchor; Transmembrane; Transmembrane helix; Virion.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000255"
FT   CHAIN           2..236
FT                   /note="Inner membrane protein pE248R"
FT                   /id="PRO_0000373606"
FT   TOPO_DOM        2..199
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:Q65200"
FT   TRANSMEM        200..220
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        221..236
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000250|UniProtKB:Q65200"
FT   LIPID           2
FT                   /note="N-myristoyl glycine; by host"
FT                   /evidence="ECO:0000250|UniProtKB:Q65200"
SQ   SEQUENCE   236 AA;  26331 MW;  94F10C862022ED4C CRC64;
     MGGSTSKYSF KNTTNIISHS IFNQMQNCIS MLDGKNYIGV FGDGNIINHV FQDLNLSLNT
     SCVQKHVNEE NFITNLSNQI TQNLKDQEVA LTQWMDAGHH DQKTDIEENI KVNLKTTLIQ
     NCVSALSGMN VLVVKGNGNI VENATQKQSQ QIISNCLQGS KQAIDTTTGI TNTVNQYSHY
     TSKNFFDFIA DAISAVFKNI MVAAVVIVLI IVGFIAVFYF LHSRTAMRRR KKLNRS