ID   E2F1_CHICK              Reviewed;         403 AA.
AC   Q90977;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 122.
DE   RecName: Full=Transcription factor E2F1 {ECO:0000303|PubMed:7478572};
DE            Short=E2F-1 {ECO:0000303|PubMed:7478572};
GN   Name=E2F1 {ECO:0000303|PubMed:7478572};
OS   Gallus gallus (Chicken).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC   Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC   Phasianinae; Gallus.
OX   NCBI_TaxID=9031;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=7478572;
RA   Pasteau S., Loiseau L., Arnaud L., Trembleau A., Brun G.;
RT   "Isolation and characterization of a chicken homolog of the E2F-1
RT   transcription factor.";
RL   Oncogene 11:1475-1486(1995).
CC   -!- FUNCTION: Transcription activator that binds DNA cooperatively with DP
CC       proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in
CC       the promoter region of a number of genes whose products are involved in
CC       cell cycle regulation or in DNA replication. The DRTF1/E2F complex
CC       functions in the control of cell-cycle progression from G1 to S phase.
CC       E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can
CC       mediate both cell proliferation and TP53/p53-dependent apoptosis.
CC       Blocks adipocyte differentiation by binding to specific promoters
CC       repressing CEBPA binding to its target gene promoters. Positively
CC       regulates transcription of RRP1B. {ECO:0000250|UniProtKB:Q01094,
CC       ECO:0000250|UniProtKB:Q61501}.
CC   -!- SUBUNIT: Component of the DRTF1/E2F transcription factor complex. Forms
CC       heterodimers with DP family members. The E2F1 complex binds
CC       specifically hypophosphorylated retinoblastoma protein RB1. During the
CC       cell cycle, RB1 becomes phosphorylated in mid-to-late G1 phase,
CC       detaches from the DRTF1/E2F complex, rendering E2F transcriptionally
CC       active. Viral oncoproteins, notably E1A, T-antigen and HPV E7, are
CC       capable of sequestering RB1, thus releasing the active complex.
CC       {ECO:0000250|UniProtKB:Q01094}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q01094}.
CC   -!- SIMILARITY: Belongs to the E2F/DP family. {ECO:0000305}.
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DR   EMBL; X89245; CAA61533.1; -; mRNA.
DR   PIR; S58345; S58345.
DR   RefSeq; NP_990550.1; NM_205219.1.
DR   AlphaFoldDB; Q90977; -.
DR   SMR; Q90977; -.
DR   STRING; 9031.ENSGALP00000042464; -.
DR   PaxDb; Q90977; -.
DR   GeneID; 396142; -.
DR   KEGG; gga:396142; -.
DR   CTD; 1869; -.
DR   VEuPathDB; HostDB:geneid_396142; -.
DR   eggNOG; KOG2577; Eukaryota.
DR   InParanoid; Q90977; -.
DR   OrthoDB; 1087250at2759; -.
DR   PhylomeDB; Q90977; -.
DR   PRO; PR:Q90977; -.
DR   Proteomes; UP000000539; Unplaced.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0005667; C:transcription regulator complex; IEA:InterPro.
DR   GO; GO:0001216; F:DNA-binding transcription activator activity; ISS:UniProtKB.
DR   GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IEA:InterPro.
DR   GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR   CDD; cd14660; E2F_DD; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR015633; E2F.
DR   InterPro; IPR037241; E2F-DP_heterodim.
DR   InterPro; IPR032198; E2F_CC-MB.
DR   InterPro; IPR003316; E2F_WHTH_DNA-bd_dom.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   PANTHER; PTHR12081; PTHR12081; 1.
DR   Pfam; PF16421; E2F_CC-MB; 1.
DR   Pfam; PF02319; E2F_TDP; 1.
DR   SMART; SM01372; E2F_TDP; 1.
DR   SUPFAM; SSF144074; SSF144074; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
PE   2: Evidence at transcript level;
KW   Activator; Cell cycle; DNA-binding; Nucleus; Reference proteome;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..403
FT                   /note="Transcription factor E2F1"
FT                   /id="PRO_0000219460"
FT   DNA_BIND        87..171
FT                   /evidence="ECO:0000255"
FT   REGION          44..85
FT                   /note="Cyclin A/CDK2 binding"
FT                   /evidence="ECO:0000255"
FT   REGION          45..64
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          130..151
FT                   /note="Leucine-zipper"
FT   REGION          172..261
FT                   /note="Dimerization"
FT                   /evidence="ECO:0000255"
FT   REGION          335..403
FT                   /note="Transactivation"
FT                   /evidence="ECO:0000255"
FT   REGION          375..392
FT                   /note="Retinoblastoma protein RB1 binding"
FT                   /evidence="ECO:0000255"
FT   MOTIF           135..171
FT                   /note="DEF box"
SQ   SEQUENCE   403 AA;  43552 MW;  78EEA320537C33ED CRC64;
     MATAGGAAGL AALLGGASPH LLIVSASEEP AGGCRPDADL LLFATPQPSR PGPAPRRPAL
     GRPPVKRKLN LETDHQYIAE SLPAARGRAR IPGRGAKSPG EKSRYETSLN LTTKRFLELL
     SQSPDGVVDL NWAAEVLKVQ KRRIYDITNV LEGIQLITKK SKNNIQWLGS QVAAGASSRQ
     RLLEKELRDL QAAERQLDDL IQTCTVRLRL LTEDPSNQHA AYVTCQDLRS IVDPSEQMVM
     VIKAPPETQL QVSDPGEAFQ VSVRSTQGPI DVFLCPEDSS GVCSPVKSPF KAPAEELSPG
     SSQQRASPLL HSAQDVNMLL PEALLPGTAL PTKCPTEDVS LSPLASMDTL LEHGKDDFPG
     FLADEFIALS PPQPQDYHFG LEEGEGISEL FDCDFGDFTH LDF