E2F2_MOUSE
ID E2F2_MOUSE Reviewed; 443 AA.
AC P56931; A2AW43; Q8BID0;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 30-AUG-2005, sequence version 2.
DT 03-AUG-2022, entry version 167.
DE RecName: Full=Transcription factor E2F2;
DE Short=E2F-2;
GN Name=E2f2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Eye;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PRELIMINARY NUCLEOTIDE SEQUENCE OF 49-254, AND DEVELOPMENTAL STAGE.
RC TISSUE=Embryo;
RX PubMed=9149906;
RA Dagnino L., Fry C.J., Bartley S.M., Farnham P., Gallie B.L., Phillips R.A.;
RT "Expression patterns of the E2F family of transcription factors during
RT murine epithelial development.";
RL Cell Growth Differ. 8:553-563(1997).
RN [5]
RP DEVELOPMENTAL STAGE.
RX PubMed=9376316; DOI=10.1016/s0925-4773(97)00083-x;
RA Dagnino L., Fry C.J., Bartley S.M., Farnham P., Gallie B.L., Phillips R.A.;
RT "Expression patterns of the E2F family of transcription factors during
RT mouse nervous system development.";
RL Mech. Dev. 66:13-25(1997).
RN [6]
RP INTERACTION WITH EAPP.
RX PubMed=15716352; DOI=10.1091/mbc.e04-11-0975;
RA Novy M., Pohn R., Andorfer P., Novy-Weiland T., Galos B., Schwarzmayr L.,
RA Rotheneder H.;
RT "EAPP, a novel E2F binding protein that modulates E2F-dependent
RT transcription.";
RL Mol. Biol. Cell 16:2181-2190(2005).
CC -!- FUNCTION: Transcription activator that binds DNA cooperatively with DP
CC proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in
CC the promoter region of a number of genes whose products are involved in
CC cell cycle regulation or in DNA replication. The DRTF1/E2F complex
CC functions in the control of cell-cycle progression from g1 to s phase.
CC E2F2 binds specifically to RB1 in a cell-cycle dependent manner.
CC -!- SUBUNIT: Component of the DRTF1/E2F transcription factor complex. Forms
CC heterodimers with DP family members. The E2F2 complex binds
CC specifically hypophosphorylated retinoblastoma protein RB1. During the
CC cell cycle, RB1 becomes phosphorylated in mid-to-late G1 phase,
CC detaches from the DRTF1/E2F complex, rendering E2F transcriptionally
CC active. Viral oncoproteins, notably E1A, T-antigen and HPV E7, are
CC capable of sequestering RB protein, thus releasing the active complex.
CC Binds EAPP.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- DEVELOPMENTAL STAGE: Expressed in the developing epidermis and
CC intestinal epithelium. First detected in the epidermis at stage 13.5-
CC 14.5 dpc with higher levels in the head and thorax regions. At 15.5
CC dpc, expression is found in both the epithelium and, to a lesser extent
CC in the underlying mesenchyme. At day 16.5 dpc, high expression in the
CC basal cells. Later expression is found in the developing hair
CC follicles, around the dermal papillae. In the developing intestinal
CC epithelium, expression first observed around 14.5 dpc. Levels continue
CC to increase at least until 19.5 dpc, with highest levels in the
CC intervillus epithelium and in the bottom half of the villi. In the
CC nervous system, first expressed at 9.5 dpc, in the forebrain. At 10.5
CC dpc, expressed broadly in the brain, and at lower levels in the upper
CC regions of the spinal cord. By 11.5 dpc, E2F2 expression is found
CC throughout the central nervous system and levels peak at 12.5-15.5 dpc.
CC In the developing spinal cord, E2F2 expression found only in the dorsal
CC region. In the developing retina, highest expression found in the 14.5-
CC 18.5 dpc embryonic retinoblastic cell layer. In other developing
CC tissues, E2F2 is found highest in thymus and liver, with lower
CC expression in lung, heart, kidney and skeletal muscle. Also found in
CC choroid plexus and chondrocytes. {ECO:0000269|PubMed:9149906,
CC ECO:0000269|PubMed:9376316}.
CC -!- PTM: Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the E2F/DP family. {ECO:0000305}.
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DR EMBL; AK087452; BAC39881.1; -; mRNA.
DR EMBL; AL935264; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC062101; AAH62101.1; -; mRNA.
DR CCDS; CCDS18801.1; -.
DR RefSeq; NP_001292328.1; NM_001305399.1.
DR RefSeq; NP_808401.1; NM_177733.7.
DR AlphaFoldDB; P56931; -.
DR SMR; P56931; -.
DR BioGRID; 232447; 3.
DR ComplexPortal; CPX-176; E2F2-DP1 transcription factor complex.
DR ComplexPortal; CPX-177; RB1-E2F2-TFDP1 transcription repressor complex.
DR CORUM; P56931; -.
DR DIP; DIP-48416N; -.
DR IntAct; P56931; 3.
DR STRING; 10090.ENSMUSP00000050047; -.
DR iPTMnet; P56931; -.
DR PhosphoSitePlus; P56931; -.
DR EPD; P56931; -.
DR jPOST; P56931; -.
DR PaxDb; P56931; -.
DR PRIDE; P56931; -.
DR ProteomicsDB; 277699; -.
DR Antibodypedia; 4274; 350 antibodies from 36 providers.
DR DNASU; 242705; -.
DR Ensembl; ENSMUST00000061721; ENSMUSP00000050047; ENSMUSG00000018983.
DR GeneID; 242705; -.
DR KEGG; mmu:242705; -.
DR UCSC; uc008vht.3; mouse.
DR CTD; 1870; -.
DR MGI; MGI:1096341; E2f2.
DR VEuPathDB; HostDB:ENSMUSG00000018983; -.
DR eggNOG; KOG2577; Eukaryota.
DR GeneTree; ENSGT00940000160992; -.
DR HOGENOM; CLU_032091_0_0_1; -.
DR InParanoid; P56931; -.
DR OMA; WVGRGIF; -.
DR OrthoDB; 1087250at2759; -.
DR PhylomeDB; P56931; -.
DR TreeFam; TF105566; -.
DR Reactome; R-MMU-69231; Cyclin D associated events in G1.
DR BioGRID-ORCS; 242705; 7 hits in 59 CRISPR screens.
DR ChiTaRS; E2f2; mouse.
DR PRO; PR:P56931; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; P56931; protein.
DR Bgee; ENSMUSG00000018983; Expressed in blood and 166 other tissues.
DR Genevisible; P56931; MM.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0035189; C:Rb-E2F complex; IC:ComplexPortal.
DR GO; GO:0090575; C:RNA polymerase II transcription regulator complex; ISO:MGI.
DR GO; GO:0000987; F:cis-regulatory region sequence-specific DNA binding; ISO:MGI.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; ISO:MGI.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:MGI.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; ISO:MGI.
DR GO; GO:0043565; F:sequence-specific DNA binding; ISO:MGI.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR GO; GO:0072332; P:intrinsic apoptotic signaling pathway by p53 class mediator; IDA:MGI.
DR GO; GO:1990086; P:lens fiber cell apoptotic process; IDA:MGI.
DR GO; GO:0043433; P:negative regulation of DNA-binding transcription factor activity; IC:ComplexPortal.
DR GO; GO:1903671; P:negative regulation of sprouting angiogenesis; ISO:MGI.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISO:MGI.
DR GO; GO:0051726; P:regulation of cell cycle; IDA:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0000083; P:regulation of transcription involved in G1/S transition of mitotic cell cycle; IC:ComplexPortal.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IDA:MGI.
DR CDD; cd14660; E2F_DD; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR015633; E2F.
DR InterPro; IPR037241; E2F-DP_heterodim.
DR InterPro; IPR032198; E2F_CC-MB.
DR InterPro; IPR003316; E2F_WHTH_DNA-bd_dom.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR12081; PTHR12081; 1.
DR Pfam; PF16421; E2F_CC-MB; 1.
DR Pfam; PF02319; E2F_TDP; 1.
DR SMART; SM01372; E2F_TDP; 1.
DR SUPFAM; SSF144074; SSF144074; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
PE 1: Evidence at protein level;
KW Activator; Cell cycle; DNA-binding; Nucleus; Phosphoprotein;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..443
FT /note="Transcription factor E2F2"
FT /id="PRO_0000219465"
FT DNA_BIND 109..198
FT /evidence="ECO:0000255"
FT REGION 1..22
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 67..107
FT /note="Cyclin A/CDK2 binding"
FT /evidence="ECO:0000255"
FT REGION 157..178
FT /note="Leucine-zipper"
FT REGION 199..291
FT /note="Dimerization"
FT /evidence="ECO:0000255"
FT REGION 306..341
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 361..443
FT /note="Transactivation"
FT /evidence="ECO:0000255"
FT REGION 416..433
FT /note="Retinoblastoma protein binding"
FT /evidence="ECO:0000255"
FT MOTIF 162..198
FT /note="DEF box"
SQ SEQUENCE 443 AA; 48499 MW; DFC8158E5A8254F0 CRC64;
MLRAPRTLAP ATAQPTKSLP ALNPTELWPS GLSSPQLCPA TTATTYYTSL YTQTVPSSVA
LGTCLDATPH GPEGQIVRCA PAGRLPAKRK LDLEGIGRPT VPEFRTPKGK CIRVDGLPSP
KTPKSPGEKT RYDTSLGLLT KKFIYLLSES EDGVLDLNWA AEVLDVQKRR IYDITNVLEG
IQLIRKKSKN NIQWVGRELF EDPTRPSRQQ QLGQELKELM NAEQTLDQLI QSCSLSFKHL
TEDNANKKLA YVTYQDIRAV GNFKEQTVIA VKAPPQTRLE VPDRAEENLQ IYLKSTQGPI
EVYLCPEEGQ EPDSPAKEAL PSTSALSPIP DCAQPGCSTD SGIAETIEPS VLIPQPIPPP
PPPPLPPAPS LVPLEATDNM LELSHPLLQQ TEDQFLSPIL AANSPLISFS PPLDQDEYLW
GMDEGEGISD LFDSYDLGDL LIN
