ID   E2F8_XENTR              Reviewed;         736 AA.
AC   F7EA39;
DT   09-JAN-2013, integrated into UniProtKB/Swiss-Prot.
DT   27-JUL-2011, sequence version 1.
DT   03-AUG-2022, entry version 58.
DE   RecName: Full=Transcription factor E2F8;
DE            Short=E2F-8;
GN   Name=e2f8;
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=20431018; DOI=10.1126/science.1183670;
RA   Hellsten U., Harland R.M., Gilchrist M.J., Hendrix D., Jurka J.,
RA   Kapitonov V., Ovcharenko I., Putnam N.H., Shu S., Taher L., Blitz I.L.,
RA   Blumberg B., Dichmann D.S., Dubchak I., Amaya E., Detter J.C., Fletcher R.,
RA   Gerhard D.S., Goodstein D., Graves T., Grigoriev I.V., Grimwood J.,
RA   Kawashima T., Lindquist E., Lucas S.M., Mead P.E., Mitros T., Ogino H.,
RA   Ohta Y., Poliakov A.V., Pollet N., Robert J., Salamov A., Sater A.K.,
RA   Schmutz J., Terry A., Vize P.D., Warren W.C., Wells D., Wills A.,
RA   Wilson R.K., Zimmerman L.B., Zorn A.M., Grainger R., Grammer T.,
RA   Khokha M.K., Richardson P.M., Rokhsar D.S.;
RT   "The genome of the Western clawed frog Xenopus tropicalis.";
RL   Science 328:633-636(2010).
CC   -!- FUNCTION: Atypical E2F transcription factor that participates in
CC       various processes such as angiogenesis and polyploidization of
CC       specialized cells. Mainly acts as a transcription repressor that binds
CC       DNA independently of DP proteins and specifically recognizes the E2
CC       recognition site 5'-TTTC[CG]CGC-3'. Directly represses transcription of
CC       classical E2F transcription factors such as e2f1. Acts as a regulator
CC       of S-phase by recognizing and binding the E2-related site 5'-TTCCCGCC-
CC       3' and mediating repression of G1/S-regulated genes. Acts as a promoter
CC       of sprouting angiogenesis, possibly by acting as a transcription
CC       activator (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer and heterodimer: mainly forms homodimers and, to a
CC       lesser extent, heterodimers with e2f7. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- DOMAIN: In contrast to classical members of the E2F transcription
CC       factor, atypical members contain 2 DNA-binding domains and regulate
CC       transcription in a DP-independent manner. Both DNA-binding domains are
CC       required for DNA-binding and are proposed to form an intramolecular
CC       structure that is similar to the winged helix structure of the E2F-DP
CC       heterodimer (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the E2F/DP family. {ECO:0000305}.
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DR   EMBL; AAMC01129757; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   AlphaFoldDB; F7EA39; -.
DR   SMR; F7EA39; -.
DR   PaxDb; F7EA39; -.
DR   eggNOG; KOG2578; Eukaryota.
DR   HOGENOM; CLU_014845_2_0_1; -.
DR   InParanoid; F7EA39; -.
DR   OMA; NGHTEMC; -.
DR   TreeFam; TF105567; -.
DR   Proteomes; UP000008143; Genome assembly.
DR   Proteomes; UP000790000; Unplaced.
DR   GO; GO:0090575; C:RNA polymerase II transcription regulator complex; IBA:GO_Central.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:UniProtKB.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0001217; F:DNA-binding transcription repressor activity; ISS:UniProtKB.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0033301; P:cell cycle comprising mitosis without cytokinesis; ISS:UniProtKB.
DR   GO; GO:0060718; P:chorionic trophoblast cell differentiation; ISS:UniProtKB.
DR   GO; GO:0070365; P:hepatocyte differentiation; ISS:UniProtKB.
DR   GO; GO:0032466; P:negative regulation of cytokinesis; ISS:UniProtKB.
DR   GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR   GO; GO:0032877; P:positive regulation of DNA endoreduplication; ISS:UniProtKB.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   GO; GO:0002040; P:sprouting angiogenesis; ISS:UniProtKB.
DR   Gene3D; 1.10.10.10; -; 2.
DR   InterPro; IPR015633; E2F.
DR   InterPro; IPR003316; E2F_WHTH_DNA-bd_dom.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   PANTHER; PTHR12081; PTHR12081; 1.
DR   Pfam; PF02319; E2F_TDP; 2.
DR   SMART; SM01372; E2F_TDP; 2.
DR   SUPFAM; SSF46785; SSF46785; 2.
PE   3: Inferred from homology;
KW   Activator; Cell cycle; DNA-binding; Nucleus; Reference proteome; Repressor;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..736
FT                   /note="Transcription factor E2F8"
FT                   /id="PRO_0000420711"
FT   DNA_BIND        98..167
FT                   /evidence="ECO:0000255"
FT   DNA_BIND        240..326
FT                   /evidence="ECO:0000255"
FT   REGION          1..26
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          386..405
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          435..456
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          483..551
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          716..736
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        11..26
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        388..405
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        513..549
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   736 AA;  80331 MW;  F2388BE4FDD2E7E5 CRC64;
     MEEGSKENCG FNGSPMGSRS PPKQLTSAAS VLGEIQIAAA NLKTPTKPQE RNNADPWTPT
     ANLKMLISAA SPEIRNRERE ILEEQFSGDE LEKTLPSRKE KSLGLLCHKF LARYPSYPNP
     AVNNSICLDE VAGELSVERR RIYDIVNVLE SLHMVSRLAK NKYIWHGRLN LSKTFDALKK
     VGEENRYGEQ IQLLRKREQE ECDSQNSPNA ETQKPLAKQP EVGFVELPGL EFRAASVNSR
     KEKSLRVMSQ RFVMLFLVSD PQIVSLEVAA KILIGEDQLE DLDKSKFKTK IRRLYDIANV
     LTSLNLIKKV HVTEEKGRKP AFQWTCPELC TDDQENRSSP AALTPVAIDL SSPKENCAKN
     LFASGGKTFT RHPSLIKLAK SIENDRRKIN SAPSSPIKSG DGSSSAASKM AQLAAICKQQ
     LQQSRDQTKV KLKVSACKAK STVKQPGGSD KNQTPTYCRA IPLLHPHPSA APPYTVIVQP
     PQEQTLSRQS PPALGYTNRT PPEAPLQGGR HEGDGTSHSE DHSAQERHPK RLPESDRGCT
     SKRMKSSAVD DVTETLYPSG YLIPIHLAPV APEPSKENTG PSSENKLFTS PIPGVFPLKL
     MFSPGPVTAV PVMSRGGQHV GGGSGSASRS PSPGMFTFAL QNRELISAGL PQGATVSPRN
     GRGQEELSAA SVLNCKHVSP VPYHGQPFTV FALQQSAVPV TPKGYHSLQE TFFRTPGGMG
     CSPPESARKL DVGTDD