E2FD_ARATH
ID E2FD_ARATH Reviewed; 359 AA.
AC Q9LFQ9;
DT 05-APR-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 25-MAY-2022, entry version 135.
DE RecName: Full=E2F transcription factor-like E2FD;
DE AltName: Full=DP-E2F-like protein 2;
DE AltName: Full=E2F-like repressor E2L1;
GN Name=E2FD; Synonyms=DEL2, E2L1, ELP3; OrderedLocusNames=At5g14960;
GN ORFNames=F2G14.80;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, GENE FAMILY, AND
RP NOMENCLATURE.
RX PubMed=11786543; DOI=10.1074/jbc.m110616200;
RA Mariconti L., Pellegrini B., Cantoni R., Stevens R., Bergounioux C.,
RA Cella R., Albani D.;
RT "The E2F family of transcription factors from Arabidopsis thaliana. Novel
RT and conserved components of the retinoblastoma/E2F pathway in plants.";
RL J. Biol. Chem. 277:9911-9919(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, AND
RP TISSUE SPECIFICITY.
RX PubMed=11867638; DOI=10.1074/jbc.m200913200;
RA Kosugi S., Ohashi Y.;
RT "E2Ls, E2F-like repressors of Arabidopsis that bind to E2F sites in a
RT monomeric form.";
RL J. Biol. Chem. 277:16553-16558(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Fujita M., Mizukado S., Seki M., Shinozaki K., Mitsuda N., Takiguchi Y.,
RA Takagi M.;
RT "ORF cloning and analysis of Arabidopsis transcription factor genes.";
RL Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11971144; DOI=10.1105/tpc.010445;
RA Vandepoele K., Raes J., de Veylder L., Rouze P., Rombauts S., Inze D.;
RT "Genome-wide analysis of core cell cycle genes in Arabidopsis.";
RL Plant Cell 14:903-916(2002).
RN [7]
RP FUNCTION, INDUCTION BY AUXIN, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=19937368; DOI=10.1007/s11103-009-9577-8;
RA Sozzani R., Maggio C., Giordo R., Umana E., Ascencio-Ibanez J.T.,
RA Hanley-Bowdoin L., Bergounioux C., Cella R., Albani D.;
RT "The E2FD/DEL2 factor is a component of a regulatory network controlling
RT cell proliferation and development in Arabidopsis.";
RL Plant Mol. Biol. 72:381-395(2010).
CC -!- FUNCTION: Inhibitor of E2F-dependent regulation of gene expression.
CC Binds specifically the E2 recognition site as a monomer without
CC interacting with DP proteins. May be up-regulating E2FA and down-
CC regulating repressors of cell cycle progression. Promotes cell
CC proliferation and represses cell elongation. Regulated by proteolysis
CC via a ubiquitin-proteasome pathway. {ECO:0000269|PubMed:11786543,
CC ECO:0000269|PubMed:11867638, ECO:0000269|PubMed:19937368}.
CC -!- SUBUNIT: Monomer. No interactions with DPA or E2FA.
CC {ECO:0000269|PubMed:11867638}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:11867638}.
CC -!- TISSUE SPECIFICITY: Preferentially expressed in proliferating tissues.
CC Highly expressed in young stalk and young flowers. Lower expression in
CC young leaves and mature flowers. Detected in cotyledonary vascular
CC tissues, the shoot apical meristem, the base of trichomes, the fully
CC developed stomata, the central root cylinder and in the columella of
CC lateral roots but not in the primary root tips or in the leaf epidermal
CC cells. {ECO:0000269|PubMed:11867638, ECO:0000269|PubMed:19937368}.
CC -!- DEVELOPMENTAL STAGE: Expressed in a cell cycle-dependent manner. Not
CC detected at the G1/S transition, but increases during the progression
CC into S phase and peaks after the passage into G2.
CC {ECO:0000269|PubMed:11786543}.
CC -!- INDUCTION: Down-regulated posttranscroptionally by auxin.
CC {ECO:0000269|PubMed:19937368}.
CC -!- DOMAIN: The two DNA binding domains are required for binding to the E2
CC site.
CC -!- DISRUPTION PHENOTYPE: Increased root length and smaller cotyledons.
CC Reduced root meristematic zone but longer cells in the differentiation
CC zone. {ECO:0000269|PubMed:19937368}.
CC -!- SIMILARITY: Belongs to the E2F/DP family. {ECO:0000305}.
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DR EMBL; AJ417835; CAD10632.1; -; mRNA.
DR EMBL; AB074531; BAB91412.1; -; mRNA.
DR EMBL; AL391146; CAC01815.1; -; Genomic_DNA.
DR EMBL; CP002688; AED92096.1; -; Genomic_DNA.
DR EMBL; AB493750; BAH30588.1; -; mRNA.
DR PIR; T51441; T51441.
DR RefSeq; NP_197000.1; NM_121500.4.
DR AlphaFoldDB; Q9LFQ9; -.
DR SMR; Q9LFQ9; -.
DR BioGRID; 16625; 9.
DR STRING; 3702.AT5G14960.1; -.
DR PaxDb; Q9LFQ9; -.
DR PRIDE; Q9LFQ9; -.
DR ProteomicsDB; 221958; -.
DR DNASU; 831348; -.
DR EnsemblPlants; AT5G14960.1; AT5G14960.1; AT5G14960.
DR GeneID; 831348; -.
DR Gramene; AT5G14960.1; AT5G14960.1; AT5G14960.
DR KEGG; ath:AT5G14960; -.
DR Araport; AT5G14960; -.
DR TAIR; locus:2147865; AT5G14960.
DR eggNOG; KOG2578; Eukaryota.
DR HOGENOM; CLU_041969_1_0_1; -.
DR InParanoid; Q9LFQ9; -.
DR OMA; EQCDSKS; -.
DR OrthoDB; 706632at2759; -.
DR PhylomeDB; Q9LFQ9; -.
DR PRO; PR:Q9LFQ9; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9LFQ9; baseline and differential.
DR Genevisible; Q9LFQ9; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0090575; C:RNA polymerase II transcription regulator complex; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; IMP:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0009733; P:response to auxin; IEP:UniProtKB.
DR Gene3D; 1.10.10.10; -; 2.
DR InterPro; IPR015633; E2F.
DR InterPro; IPR003316; E2F_WHTH_DNA-bd_dom.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR12081; PTHR12081; 1.
DR Pfam; PF02319; E2F_TDP; 2.
DR SMART; SM01372; E2F_TDP; 2.
DR SUPFAM; SSF46785; SSF46785; 2.
PE 1: Evidence at protein level;
KW Activator; Cell cycle; DNA-binding; Nucleus; Reference proteome; Repressor;
KW Transcription; Transcription regulation.
FT CHAIN 1..359
FT /note="E2F transcription factor-like E2FD"
FT /id="PRO_0000406292"
FT DNA_BIND 13..78
FT DNA_BIND 138..217
FT REGION 255..274
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 288..313
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 291..310
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 359 AA; 40553 MW; 9E14C2933E07CF3C CRC64;
MDSLALAPQV YSRKDKSLGV LVANFLTLYN RPDVDLFGLD DAAAKLGVER RRIYDVVNIL
ESIGLVARSG KNQYSWKGFG AVPRALSELK EEGMKEKFAI VPFVAKSEMV VYEKEGEESF
MLSPDDQEFS PSPRPDNRKE RTLWLLAQNF VKLFLCSDDD LVTFDSATKA LLNESQDMNM
RKKVRRLYDI ANVFSSMKLI EKTHVPETKK PAYRWLGSKT IFENRFIDGS ASLCDRNVPK
KRAFGTELTN VNAKRNKSGC SKEDSKRNGN QNTSIVIKQE QCDDVKPDVK NFASGSSTPA
GTSESNDMGN NIRPRGRLGV IEALSTLYQP SYCNPELLGL FAHYNETFRS YQEEFGREK
