E2FF_ARATH
ID E2FF_ARATH Reviewed; 354 AA.
AC Q8RWL0; Q9SRI0;
DT 05-APR-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=E2F transcription factor-like E2FF;
DE AltName: Full=DP-E2F-like protein 3;
DE AltName: Full=E2F-like repressor E2L2;
GN Name=E2FF; Synonyms=DEL3, E2L2, ELP1; OrderedLocusNames=At3g01330;
GN ORFNames=T22N4.4;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, AND TISSUE
RP SPECIFICITY.
RX PubMed=11867638; DOI=10.1074/jbc.m200913200;
RA Kosugi S., Ohashi Y.;
RT "E2Ls, E2F-like repressors of Arabidopsis that bind to E2F sites in a
RT monomeric form.";
RL J. Biol. Chem. 277:16553-16558(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP FUNCTION, DEVELOPMENTAL STAGE, GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11786543; DOI=10.1074/jbc.m110616200;
RA Mariconti L., Pellegrini B., Cantoni R., Stevens R., Bergounioux C.,
RA Cella R., Albani D.;
RT "The E2F family of transcription factors from Arabidopsis thaliana. Novel
RT and conserved components of the retinoblastoma/E2F pathway in plants.";
RL J. Biol. Chem. 277:9911-9919(2002).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11971144; DOI=10.1105/tpc.010445;
RA Vandepoele K., Raes J., de Veylder L., Rouze P., Rombauts S., Inze D.;
RT "Genome-wide analysis of core cell cycle genes in Arabidopsis.";
RL Plant Cell 14:903-916(2002).
RN [7]
RP FUNCTION, DEVELOPMENTAL STAGE, AND TISSUE SPECIFICITY.
RX PubMed=15308755; DOI=10.1105/tpc.104.023978;
RA Ramirez-Parra E., Lopez-Matas M.A., Fruendt C., Gutierrez C.;
RT "Role of an atypical E2F transcription factor in the control of Arabidopsis
RT cell growth and differentiation.";
RL Plant Cell 16:2350-2363(2004).
CC -!- FUNCTION: Inhibitor of E2F-dependent activation of gene expression.
CC Binds specifically the E2 recognition site without interacting with DP
CC proteins and prevents transcription activation by E2F/DP heterodimers.
CC Does not bind retinoblastoma-related proteins. Acts as a growth
CC regulator but is not associated with changes in the expression of cell
CC cycle marker genes or in nuclear ploidy levels. Has no effect on cell
CC proliferation, but may repress cell wall biosynthesis genes during cell
CC elongation in differentiated cells. {ECO:0000269|PubMed:11786543,
CC ECO:0000269|PubMed:11867638, ECO:0000269|PubMed:15308755}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:11867638}. Cytoplasm
CC {ECO:0000269|PubMed:11867638}.
CC -!- TISSUE SPECIFICITY: High expression in young cotyledons and leaves,
CC hypocotyls, shoot apical meristem, roots and mature pollen grains,
CC moderate in developing trichomes, flowers and at early stages of
CC developing anthers, and barely detectable in mature leaves. Not
CC detected in primary root meristem, emerging lateral roots, pistils,
CC developing embryos and siliques. {ECO:0000269|PubMed:11867638,
CC ECO:0000269|PubMed:15308755}.
CC -!- DEVELOPMENTAL STAGE: Expressed in a cell cycle-dependent manner. Most
CC abundant in mid-S phase. {ECO:0000269|PubMed:11786543,
CC ECO:0000269|PubMed:15308755}.
CC -!- SIMILARITY: Belongs to the E2F/DP family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF03493.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB074532; BAB91413.1; -; mRNA.
DR EMBL; AC010676; AAF03493.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE73650.1; -; Genomic_DNA.
DR EMBL; AY093024; AAM13023.1; -; mRNA.
DR EMBL; BT000412; AAN15731.1; -; mRNA.
DR RefSeq; NP_186782.2; NM_110999.7.
DR AlphaFoldDB; Q8RWL0; -.
DR SMR; Q8RWL0; -.
DR BioGRID; 6554; 4.
DR IntAct; Q8RWL0; 1.
DR STRING; 3702.AT3G01330.1; -.
DR PaxDb; Q8RWL0; -.
DR PRIDE; Q8RWL0; -.
DR ProteomicsDB; 222011; -.
DR EnsemblPlants; AT3G01330.1; AT3G01330.1; AT3G01330.
DR GeneID; 821221; -.
DR Gramene; AT3G01330.1; AT3G01330.1; AT3G01330.
DR KEGG; ath:AT3G01330; -.
DR Araport; AT3G01330; -.
DR TAIR; locus:2100272; AT3G01330.
DR eggNOG; KOG2578; Eukaryota.
DR HOGENOM; CLU_041969_1_0_1; -.
DR InParanoid; Q8RWL0; -.
DR OMA; EDSQWKN; -.
DR OrthoDB; 706632at2759; -.
DR PhylomeDB; Q8RWL0; -.
DR PRO; PR:Q8RWL0; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q8RWL0; baseline and differential.
DR Genevisible; Q8RWL0; AT.
DR GO; GO:0005737; C:cytoplasm; IDA:TAIR.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0090575; C:RNA polymerase II transcription regulator complex; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 1.10.10.10; -; 2.
DR InterPro; IPR015633; E2F.
DR InterPro; IPR003316; E2F_WHTH_DNA-bd_dom.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR12081; PTHR12081; 1.
DR Pfam; PF02319; E2F_TDP; 2.
DR SMART; SM01372; E2F_TDP; 2.
DR SUPFAM; SSF46785; SSF46785; 2.
PE 2: Evidence at transcript level;
KW Cell cycle; Cytoplasm; DNA-binding; Nucleus; Reference proteome; Repressor;
KW Transcription; Transcription regulation.
FT CHAIN 1..354
FT /note="E2F transcription factor-like E2FF"
FT /id="PRO_0000406294"
FT DNA_BIND 21..86
FT DNA_BIND 145..225
FT REGION 104..143
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 354 AA; 39999 MW; C4F423B5B34B3F90 CRC64;
MSSAIVVSQD AESLGLQIYS RKEKSLGVLV SNFLRLYNRD DVDLIGLDDA AGQLGVERRR
IYDVVNILES IGIVARRGKN QYSWKGFGEI PRSLDELKEE GMRERLGYSS SNNSDKVSNG
CEREEPLTLT PDDQENSSSS KMDQKKEKSL WLLAQNFVKM FLCSDDDLIT LDSAAKALLS
DSPDSVHMRT KVRRLYDIAN VFASMNLIEK THIPVTRKPA YRWLGSKSIA ERGLSLFNSG
EPKRVFGTEI TNLRAKRNKT YCSSIRKQIG YKKHDEENTE QESKPAASKY VFGPFSPIGA
SKTNNDKVGK GRLLEIEALA STYQPQYCNQ EITGLLGHFT EAWKKWYAEV DRNK
