E2_VACCW
ID E2_VACCW Reviewed; 737 AA.
AC P21604; Q76ZW3;
DT 01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1991, sequence version 2.
DT 25-MAY-2022, entry version 71.
DE RecName: Full=Protein E2;
GN OrderedLocusNames=VACWR058; ORFNames=E2L;
OS Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain
OS WR)).
OC Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX NCBI_TaxID=10254;
OH NCBI_TaxID=9913; Bos taurus (Bovine).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=7060133; DOI=10.1016/0092-8674(82)90349-x;
RA Baroudy B.M., Venkatesan S., Moss B.;
RT "Incompletely base-paired flip-flop terminal loops link the two DNA strands
RT of the vaccinia virus genome into one uninterrupted polynucleotide chain.";
RL Cell 28:315-324(1982).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Gershon P.D., Jones E.V., Moss B., Ahn B.-Y.;
RL Submitted (JUL-1990) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J.,
RA Wohlhueter R.;
RT "Sequencing of the coding region of Vaccinia-WR to an average 9-fold
RT redundancy and an error rate of 0.16/10kb.";
RL Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-597.
RX PubMed=2398897; DOI=10.1128/mcb.10.10.5433-5441.1990;
RA Ahn B.-Y., Gershon P.D., Jones E.V., Moss B.;
RT "Identification of rpo30, a vaccinia virus RNA polymerase gene with
RT structural similarity to a eucaryotic transcription elongation factor.";
RL Mol. Cell. Biol. 10:5433-5441(1990).
RN [5]
RP INTERACTION WITH PROTEIN F12, AND FUNCTION.
RX PubMed=19207726; DOI=10.1111/j.1462-5822.2009.01296.x;
RA Dodding M.P., Newsome T.P., Collinson L.M., Edwards C., Way M.;
RT "An E2-F12 complex is required for intracellular enveloped virus
RT morphogenesis during vaccinia infection.";
RL Cell. Microbiol. 11:808-824(2009).
RN [6]
RP FUNCTION, AND INTERACTION WITH HOST KLC2.
RX PubMed=25760349; DOI=10.1371/journal.ppat.1004723;
RA Carpentier D.C., Gao W.N., Ewles H., Morgan G.W., Smith G.L.;
RT "Vaccinia virus protein complex F12/E2 interacts with kinesin light chain
RT isoform 2 to engage the kinesin-1 motor complex.";
RL PLoS Pathog. 11:E1004723-E1004723(2015).
CC -!- FUNCTION: Late protein that plays a role in intracellular enveloped
CC virus (IEV) transport to the cell surface on microtubules. Together
CC with protein F12, forms a complex that interacts with host KLC2
CC (kinesin light chain isoform 2) to engage the kinesin-1 complex and
CC thereby promote IEV trafficking. {ECO:0000269|PubMed:19207726}.
CC -!- SUBUNIT: Interacts with host KLC2; this interaction promotes IEV
CC trafficking by engaging the host kinesin-1 complex. Interacts with
CC protein F12. {ECO:0000269|PubMed:19207726,
CC ECO:0000269|PubMed:25760349}.
CC -!- SIMILARITY: Belongs to the poxviridae E2 protein family. {ECO:0000305}.
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DR EMBL; AY243312; AAO89337.1; -; Genomic_DNA.
DR EMBL; M36339; AAB59822.1; -; Genomic_DNA.
DR PIR; A35928; A35928.
DR RefSeq; YP_232940.1; NC_006998.1.
DR PDB; 7PHY; X-ray; 2.30 A; A=1-737.
DR PDBsum; 7PHY; -.
DR SMR; P21604; -.
DR PRIDE; P21604; -.
DR DNASU; 3707591; -.
DR GeneID; 3707591; -.
DR KEGG; vg:3707591; -.
DR Proteomes; UP000000344; Genome.
DR GO; GO:0043657; C:host cell; IEA:GOC.
DR GO; GO:0039701; P:microtubule-dependent intracellular transport of viral material towards cell periphery; IDA:UniProtKB.
DR InterPro; IPR007585; Poxvirus_E2.
DR InterPro; IPR021155; Poxvirus_E2/O1.
DR Pfam; PF04497; Pox_E2-like; 1.
DR PIRSF; PIRSF015692; VAC_E2L; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Reference proteome.
FT CHAIN 1..737
FT /note="Protein E2"
FT /id="PRO_0000099444"
FT HELIX 5..13
FT /evidence="ECO:0007829|PDB:7PHY"
FT HELIX 19..24
FT /evidence="ECO:0007829|PDB:7PHY"
FT HELIX 28..36
FT /evidence="ECO:0007829|PDB:7PHY"
FT HELIX 46..48
FT /evidence="ECO:0007829|PDB:7PHY"
FT HELIX 49..55
FT /evidence="ECO:0007829|PDB:7PHY"
FT HELIX 57..62
FT /evidence="ECO:0007829|PDB:7PHY"
FT TURN 65..67
FT /evidence="ECO:0007829|PDB:7PHY"
FT HELIX 70..79
FT /evidence="ECO:0007829|PDB:7PHY"
FT HELIX 84..86
FT /evidence="ECO:0007829|PDB:7PHY"
FT HELIX 87..92
FT /evidence="ECO:0007829|PDB:7PHY"
FT HELIX 94..99
FT /evidence="ECO:0007829|PDB:7PHY"
FT HELIX 103..109
FT /evidence="ECO:0007829|PDB:7PHY"
FT HELIX 110..112
FT /evidence="ECO:0007829|PDB:7PHY"
FT HELIX 117..126
FT /evidence="ECO:0007829|PDB:7PHY"
FT TURN 127..129
FT /evidence="ECO:0007829|PDB:7PHY"
FT HELIX 132..137
FT /evidence="ECO:0007829|PDB:7PHY"
FT HELIX 142..146
FT /evidence="ECO:0007829|PDB:7PHY"
FT HELIX 154..163
FT /evidence="ECO:0007829|PDB:7PHY"
FT HELIX 165..167
FT /evidence="ECO:0007829|PDB:7PHY"
FT HELIX 168..174
FT /evidence="ECO:0007829|PDB:7PHY"
FT HELIX 179..189
FT /evidence="ECO:0007829|PDB:7PHY"
FT HELIX 204..213
FT /evidence="ECO:0007829|PDB:7PHY"
FT HELIX 217..230
FT /evidence="ECO:0007829|PDB:7PHY"
FT HELIX 235..246
FT /evidence="ECO:0007829|PDB:7PHY"
FT HELIX 251..253
FT /evidence="ECO:0007829|PDB:7PHY"
FT HELIX 254..260
FT /evidence="ECO:0007829|PDB:7PHY"
FT HELIX 262..264
FT /evidence="ECO:0007829|PDB:7PHY"
FT HELIX 265..268
FT /evidence="ECO:0007829|PDB:7PHY"
FT HELIX 269..271
FT /evidence="ECO:0007829|PDB:7PHY"
FT HELIX 272..275
FT /evidence="ECO:0007829|PDB:7PHY"
FT HELIX 282..284
FT /evidence="ECO:0007829|PDB:7PHY"
FT HELIX 289..296
FT /evidence="ECO:0007829|PDB:7PHY"
FT HELIX 297..301
FT /evidence="ECO:0007829|PDB:7PHY"
FT HELIX 303..305
FT /evidence="ECO:0007829|PDB:7PHY"
FT HELIX 306..315
FT /evidence="ECO:0007829|PDB:7PHY"
FT HELIX 319..324
FT /evidence="ECO:0007829|PDB:7PHY"
FT HELIX 326..328
FT /evidence="ECO:0007829|PDB:7PHY"
FT HELIX 330..332
FT /evidence="ECO:0007829|PDB:7PHY"
FT HELIX 335..344
FT /evidence="ECO:0007829|PDB:7PHY"
FT HELIX 351..353
FT /evidence="ECO:0007829|PDB:7PHY"
FT HELIX 359..367
FT /evidence="ECO:0007829|PDB:7PHY"
FT HELIX 373..378
FT /evidence="ECO:0007829|PDB:7PHY"
FT HELIX 382..387
FT /evidence="ECO:0007829|PDB:7PHY"
FT HELIX 395..398
FT /evidence="ECO:0007829|PDB:7PHY"
FT HELIX 402..405
FT /evidence="ECO:0007829|PDB:7PHY"
FT HELIX 407..417
FT /evidence="ECO:0007829|PDB:7PHY"
FT HELIX 421..429
FT /evidence="ECO:0007829|PDB:7PHY"
FT HELIX 435..446
FT /evidence="ECO:0007829|PDB:7PHY"
FT HELIX 452..454
FT /evidence="ECO:0007829|PDB:7PHY"
FT TURN 455..457
FT /evidence="ECO:0007829|PDB:7PHY"
FT HELIX 458..460
FT /evidence="ECO:0007829|PDB:7PHY"
FT HELIX 462..467
FT /evidence="ECO:0007829|PDB:7PHY"
FT STRAND 493..498
FT /evidence="ECO:0007829|PDB:7PHY"
FT HELIX 503..513
FT /evidence="ECO:0007829|PDB:7PHY"
FT STRAND 522..530
FT /evidence="ECO:0007829|PDB:7PHY"
FT STRAND 533..541
FT /evidence="ECO:0007829|PDB:7PHY"
FT HELIX 547..563
FT /evidence="ECO:0007829|PDB:7PHY"
FT STRAND 566..568
FT /evidence="ECO:0007829|PDB:7PHY"
FT TURN 571..573
FT /evidence="ECO:0007829|PDB:7PHY"
FT HELIX 579..584
FT /evidence="ECO:0007829|PDB:7PHY"
FT STRAND 593..596
FT /evidence="ECO:0007829|PDB:7PHY"
FT HELIX 603..605
FT /evidence="ECO:0007829|PDB:7PHY"
FT STRAND 608..610
FT /evidence="ECO:0007829|PDB:7PHY"
FT HELIX 611..621
FT /evidence="ECO:0007829|PDB:7PHY"
FT HELIX 630..652
FT /evidence="ECO:0007829|PDB:7PHY"
FT HELIX 657..671
FT /evidence="ECO:0007829|PDB:7PHY"
FT HELIX 683..699
FT /evidence="ECO:0007829|PDB:7PHY"
FT STRAND 701..705
FT /evidence="ECO:0007829|PDB:7PHY"
FT HELIX 712..731
FT /evidence="ECO:0007829|PDB:7PHY"
SQ SEQUENCE 737 AA; 85958 MW; D5BAF09E2F944A9E CRC64;
MISVTDIRRA FLDNECHTIT KAFGYLHEDK AIALIKIGFH PTYLPKVLYN NVVEFVPEKL
YLFKPRTVAP LDLISTITKL KNVDKFASHI NYHKNSILIT GDKSLIVKCM PYMIISDDDI
RFIREQFVGT NSIEYILSFI NKESIYRMSY QFSENEIVTI INRDHFMYEP IYEHQVLDSD
FLKTMLDRYG IVPINSGIID ELCPEAIIEI LMAVVRPRDA IRFLDIVNKN QLTEDSVKNY
IINDIRRGKI DYYIPYVEDF LEDRTEDLGI YANIFFEDAI DITKLDITKT ELEHISKYMN
YYTTYIDHIV NIILQNNYID ILASIIDYVQ DVLTEELCIR IVCESTNPVP VTSLPIHSTL
VMVMCIQMKY VDIVEFLDEI DIDTLIEKGA DPITEYTFTT RWYNKHNDLI TLYIKKYGFC
PMMMKRLMFE YPLTKEASDH LLKTMDENRG AIMFFPRTIC TLPYLLCCNY KLIQKPIPFK
EENRNIVYKK NNRVLCFDSL ENSAFKSLIK IDSIPGLKTY NMKDITYEKS NNIICVRFIP
QESIHNEERR IKLQLFDIAR LASYGLYYIP SRYLSSWTPV VNMIEGREYT NPQKIECLVI
LDLFSEEFIE YQNLGNAVSN KYELEYTISN YQAAINCLMS TLLIYLVLGS IRSISRTENF
VLSILNIFYK GLKINELLSE PVSGVCIELN KIKDRASSGD SSFIFLKKNE LSKTLSLCEK
VCVETILDNN QSFKSSK
