ADM2_RAT
ID ADM2_RAT Reviewed; 146 AA.
AC P61312; Q6L5N4;
DT 10-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2004, sequence version 1.
DT 03-AUG-2022, entry version 95.
DE RecName: Full=Protein ADM2;
DE AltName: Full=Intermedin;
DE Contains:
DE RecName: Full=Adrenomedullin-2;
DE Short=AM2;
DE AltName: Full=Intermedin-long;
DE Short=IMDL;
DE Contains:
DE RecName: Full=Intermedin-short;
DE Short=IMDS;
DE Flags: Precursor;
GN Name=Adm2; Synonyms=Am2;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Kidney;
RX PubMed=14706825; DOI=10.1016/s0014-5793(03)01368-1;
RA Takei Y., Inoue K., Ogoshi M., Kawahara T., Bannai H., Miyano S.;
RT "Identification of novel adrenomedullin in mammals: a potent cardiovascular
RT and renal regulator.";
RL FEBS Lett. 556:53-58(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Inoue K., Takei Y.;
RT "cDNA for the precursor of rat adrenomedullin 2.";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Sprague-Dawley;
RA Chang C.L., Roh J., Hsu S.Y.;
RT "Rat IMD sequence.";
RL Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=14615490; DOI=10.1074/jbc.m305332200;
RA Roh J., Chang C.L., Bhalla A., Klein C., Hsu S.Y.T.;
RT "Intermedin is a calcitonin/calcitonin gene-related peptide family peptide
RT acting through the calcitonin receptor-like receptor/receptor activity-
RT modifying protein receptor complexes.";
RL J. Biol. Chem. 279:7264-7274(2004).
RN [5]
RP CLEAVAGE OF SIGNAL PEPTIDE AFTER SER-25, AND IDENTIFICATION BY MASS
RP SPECTROMETRY.
RX PubMed=26479776; DOI=10.1021/acs.jproteome.5b00820;
RA Tsuchiya T., Osaki T., Minamino N., Sasaki K.;
RT "Peptidomics for studying limited proteolysis.";
RL J. Proteome Res. 14:4921-4931(2015).
CC -!- FUNCTION: [Adrenomedullin-2]: May play a role as physiological
CC regulators of gastrointestinal, cardiovascular bioactivities mediated
CC by the CALCRL/RAMPs receptor complexes. Activates the cAMP-dependent
CC pathway. {ECO:0000269|PubMed:14615490}.
CC -!- FUNCTION: [Intermedin-short]: May play a role as physiological
CC regulators of gastrointestinal, cardiovascular bioactivities mediated
CC by the CALCRL/RAMPs receptor complexes. Activates the cAMP-dependent
CC pathway. {ECO:0000269|PubMed:14615490}.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- TISSUE SPECIFICITY: Expression was restricted to the intermediate and
CC anterior lobes of the pituitary. {ECO:0000269|PubMed:14615490}.
CC -!- SIMILARITY: Belongs to the adrenomedullin family. {ECO:0000305}.
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DR EMBL; AB121036; BAD07413.1; -; mRNA.
DR EMBL; AB181297; BAD22678.1; -; mRNA.
DR EMBL; AY590103; AAT01302.1; -; mRNA.
DR RefSeq; NP_958829.1; NM_201426.1.
DR AlphaFoldDB; P61312; -.
DR SMR; P61312; -.
DR STRING; 10116.ENSRNOP00000051266; -.
DR PaxDb; P61312; -.
DR Ensembl; ENSRNOT00000044854; ENSRNOP00000051266; ENSRNOG00000029830.
DR GeneID; 399475; -.
DR KEGG; rno:399475; -.
DR CTD; 79924; -.
DR RGD; 1302971; Adm2.
DR eggNOG; ENOG502S7F2; Eukaryota.
DR GeneTree; ENSGT00940000154380; -.
DR HOGENOM; CLU_134508_0_0_1; -.
DR InParanoid; P61312; -.
DR OMA; RLWQLVR; -.
DR OrthoDB; 1381783at2759; -.
DR Reactome; R-RNO-419812; Calcitonin-like ligand receptors.
DR PRO; PR:P61312; -.
DR Proteomes; UP000002494; Chromosome 7.
DR Bgee; ENSRNOG00000029830; Expressed in adult mammalian kidney and 12 other tissues.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0005179; F:hormone activity; TAS:RGD.
DR GO; GO:0044877; F:protein-containing complex binding; ISO:RGD.
DR GO; GO:0007189; P:adenylate cyclase-activating G protein-coupled receptor signaling pathway; IDA:RGD.
DR GO; GO:0001525; P:angiogenesis; ISO:RGD.
DR GO; GO:0007586; P:digestion; IMP:RGD.
DR GO; GO:0007631; P:feeding behavior; IMP:RGD.
DR GO; GO:0045776; P:negative regulation of blood pressure; IMP:RGD.
DR GO; GO:0045766; P:positive regulation of angiogenesis; ISO:RGD.
DR GO; GO:0010628; P:positive regulation of gene expression; ISO:RGD.
DR GO; GO:0010460; P:positive regulation of heart rate; IBA:GO_Central.
DR GO; GO:0006468; P:protein phosphorylation; ISO:RGD.
DR GO; GO:0003073; P:regulation of systemic arterial blood pressure; IBA:GO_Central.
DR InterPro; IPR021116; Calcitonin/adrenomedullin.
DR Pfam; PF00214; Calc_CGRP_IAPP; 1.
PE 1: Evidence at protein level;
KW Amidation; Cleavage on pair of basic residues; Disulfide bond; Hormone;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..25
FT /evidence="ECO:0000269|PubMed:26479776"
FT PROPEP 26..96
FT /evidence="ECO:0000250"
FT /id="PRO_0000000983"
FT PEPTIDE 99..145
FT /note="Adrenomedullin-2"
FT /evidence="ECO:0000250"
FT /id="PRO_0000000984"
FT PEPTIDE 106..145
FT /note="Intermedin-short"
FT /evidence="ECO:0000255"
FT /id="PRO_0000000985"
FT REGION 29..99
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 39..55
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 145
FT /note="Tyrosine amide"
FT /evidence="ECO:0000250|UniProtKB:P35318"
FT DISULFID 108..113
FT /evidence="ECO:0000250"
SQ SEQUENCE 146 AA; 15572 MW; C87043237AD29DDC CRC64;
MAQLLMVTVT FGCISLLYLL PGTLSGSLGK GLRPREPPAK IPSSGPQPGH PSLRPVVWKP
PHALQPQGRG NPALATVHLP QGGGSRHPGP QRHVGSRRPH AQLLRVGCVL GTCQVQNLSH
RLWQLVRPSG RRDSAPVDPS SPHSYG
