ID   E3B14_ADE05             Reviewed;         132 AA.
AC   P06498;
DT   01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1988, sequence version 1.
DT   23-FEB-2022, entry version 87.
DE   RecName: Full=Early E3B 14.6 kDa protein;
DE   Flags: Precursor;
OS   Human adenovirus C serotype 5 (HAdV-5) (Human adenovirus 5).
OC   Viruses; Varidnaviria; Bamfordvirae; Preplasmiviricota; Tectiliviricetes;
OC   Rowavirales; Adenoviridae; Mastadenovirus.
OX   NCBI_TaxID=28285;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2981456; DOI=10.1016/0042-6822(85)90443-x;
RA   Cladaras C., Wold W.S.M.;
RT   "DNA sequence of the early E3 transcription unit of adenovirus 5.";
RL   Virology 140:28-43(1985).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=1727603; DOI=10.1016/0042-6822(92)90082-z;
RA   Chroboczek J., Bieber F., Jacrot B.;
RT   "The sequence of the genome of adenovirus type 5 and its comparison with
RT   the genome of adenovirus type 2.";
RL   Virology 186:280-285(1992).
RN   [3]
RP   IDENTIFICATION OF PROTEIN.
RX   PubMed=2309441; DOI=10.1016/0042-6822(90)90182-q;
RA   Tollefson A.E., Krajcsi P., Pursley M.H., Gooding L.R., Wold W.S.M.;
RT   "A 14,500 MW protein is coded by region E3 of group C human adenoviruses.";
RL   Virology 175:19-29(1990).
RN   [4]
RP   SIGNAL SEQUENCE CLEAVAGE SITE.
RX   PubMed=1531370; DOI=10.1128/jvi.66.3.1665-1673.1992;
RA   Krajcsi P., Tollefson A.E., Anderson C.W., Wold W.S.M.;
RT   "The adenovirus E3 14.5-kilodalton protein, which is required for down-
RT   regulation of the epidermal growth factor receptor and prevention of tumor
RT   necrosis factor cytolysis, is an integral membrane protein oriented with
RT   its C-terminus in the cytoplasm.";
RL   J. Virol. 66:1665-1673(1992).
RN   [5]
RP   PHOSPHORYLATION.
RX   PubMed=1532104; DOI=10.1016/0042-6822(92)90451-t;
RA   Krajcsi P., Wold W.S.M.;
RT   "The adenovirus E3-14.5K protein which is required for prevention of TNF
RT   cytolysis and for down-regulation of the EGF receptor contains
RT   phosphoserine.";
RL   Virology 187:492-498(1992).
RN   [6]
RP   GLYCOSYLATION.
RX   PubMed=1533979; DOI=10.1016/0042-6822(92)90511-m;
RA   Krajcsi P., Tollefson A.E., Wold W.S.M.;
RT   "The E3-14.5K integral membrane protein of adenovirus that is required for
RT   down-regulation of the EGF receptor and for prevention of TNF cytolysis is
RT   O-glycosylated but not N-glycosylated.";
RL   Virology 188:570-579(1992).
CC   -!- FUNCTION: Down-regulates the EGF receptor and prevents cytolysis by
CC       TNF.
CC   -!- INTERACTION:
CC       P06498; P42224-1: STAT1; Xeno; NbExp=2; IntAct=EBI-16085959, EBI-15711971;
CC   -!- SUBCELLULAR LOCATION: Host membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
CC   -!- PTM: Phosphorylated on serine; O-glycosylated, but not N-glycosylated.
CC       {ECO:0000269|PubMed:1532104, ECO:0000269|PubMed:1533979}.
CC   -!- SIMILARITY: Belongs to the adenoviridae E3_14 family. {ECO:0000305}.
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DR   EMBL; M73260; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; X03002; CAA26786.1; -; Genomic_DNA.
DR   RefSeq; AP_000223.1; AC_000008.1.
DR   SMR; P06498; -.
DR   DIP; DIP-61723N; -.
DR   IntAct; P06498; 1.
DR   Proteomes; UP000004992; Genome.
DR   GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0009966; P:regulation of signal transduction; IEA:InterPro.
DR   InterPro; IPR008131; Adeno_E3_14_5.
DR   Pfam; PF04834; Adeno_E3_14_5; 1.
PE   1: Evidence at protein level;
KW   Early protein; Glycoprotein; Host membrane; Membrane; Phosphoprotein;
KW   Signal; Transmembrane; Transmembrane helix.
FT   SIGNAL          1..18
FT                   /evidence="ECO:0000269|PubMed:1531370"
FT   CHAIN           19..132
FT                   /note="Early E3B 14.6 kDa protein"
FT                   /id="PRO_0000036479"
FT   TRANSMEM        51..75
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   132 AA;  14750 MW;  524690C4AD9B9A74 CRC64;
     MKFTVTFLLI ICTLSAFCSP TSKPQRHISC RFTRIWNIPS CYNEKSDLSE AWLYAIISVM
     VFCSTILALA IYPYLDIGWK RIDAMNHPTF PAPAMLPLQQ VVAGGFVPAN QPRPTSPTPT
     EISYFNLTGG DD