E3RDB_ADE02
ID E3RDB_ADE02 Reviewed; 130 AA.
AC P03250;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 21-JUL-1986, sequence version 1.
DT 02-DEC-2020, entry version 78.
DE RecName: Full=Early 3 receptor internalization and degradation beta protein;
DE Short=E3 RID-beta protein;
DE AltName: Full=Early E3B 14.5 kDa protein;
DE Short=E3-14.5k;
DE Flags: Precursor;
OS Human adenovirus C serotype 2 (HAdV-2) (Human adenovirus 2).
OC Viruses; Varidnaviria; Bamfordvirae; Preplasmiviricota; Tectiliviricetes;
OC Rowavirales; Adenoviridae; Mastadenovirus.
OX NCBI_TaxID=10515;
OH NCBI_TaxID=9606; Homo sapiens (Human).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=6262722; DOI=10.1093/nar/9.5.1229;
RA Herisse J., Galibert F.;
RT "Nucleotide sequence of the EcoRI E fragment of adenovirus 2 genome.";
RL Nucleic Acids Res. 9:1229-1240(1981).
RN [2]
RP IDENTIFICATION OF PROTEIN.
RX PubMed=2309441; DOI=10.1016/0042-6822(90)90182-q;
RA Tollefson A.E., Krajcsi P., Pursley M.H., Gooding L.R., Wold W.S.M.;
RT "A 14,500 MW protein is coded by region E3 of group C human adenoviruses.";
RL Virology 175:19-29(1990).
RN [3]
RP PHOSPHORYLATION.
RC STRAIN=Human adenovirus C serotype 5;
RX PubMed=1532104; DOI=10.1016/0042-6822(92)90451-t;
RA Krajcsi P., Wold W.S.M.;
RT "The adenovirus E3-14.5K protein which is required for prevention of TNF
RT cytolysis and for down-regulation of the EGF receptor contains
RT phosphoserine.";
RL Virology 187:492-498(1992).
RN [4]
RP GLYCOSYLATION.
RC STRAIN=Human adenovirus C serotype 5;
RX PubMed=1533979; DOI=10.1016/0042-6822(92)90511-m;
RA Krajcsi P., Tollefson A.E., Wold W.S.M.;
RT "The E3-14.5K integral membrane protein of adenovirus that is required for
RT down-regulation of the EGF receptor and for prevention of TNF cytolysis is
RT O-glycosylated but not N-glycosylated.";
RL Virology 188:570-579(1992).
RN [5]
RP TOPOLOGY.
RC STRAIN=Human adenovirus C serotype 5;
RX PubMed=1531370; DOI=10.1128/jvi.66.3.1665-1673.1992;
RA Krajcsi P., Tollefson A.E., Anderson C.W., Wold W.S.M.;
RT "The adenovirus E3 14.5-kilodalton protein, which is required for down-
RT regulation of the epidermal growth factor receptor and prevention of tumor
RT necrosis factor cytolysis, is an integral membrane protein oriented with
RT its C-terminus in the cytoplasm.";
RL J. Virol. 66:1665-1673(1992).
RN [6]
RP SUBCELLULAR LOCATION.
RC STRAIN=Human adenovirus C serotype 5;
RX PubMed=7983708; DOI=10.1128/jvi.69.1.172-181.1995;
RA Stewart A.R., Tollefson A.E., Krajcsi P., Yei S.P., Wold W.S.;
RT "The adenovirus E3 10.4K and 14.5K proteins, which function to prevent
RT cytolysis by tumor necrosis factor and to down-regulate the epidermal
RT growth factor receptor, are localized in the plasma membrane.";
RL J. Virol. 69:172-181(1995).
RN [7]
RP TYROSINE-BASED SORTING MOTIF.
RC STRAIN=Human adenovirus C serotype 5;
RX PubMed=12388693; DOI=10.1128/jvi.76.22.11329-11342.2002;
RA Lichtenstein D.L., Krajcsi P., Esteban D.J., Tollefson A.E., Wold W.S.;
RT "Adenovirus RIDbeta subunit contains a tyrosine residue that is critical
RT for RID-mediated receptor internalization and inhibition of Fas- and TRAIL-
RT induced apoptosis.";
RL J. Virol. 76:11329-11342(2002).
RN [8]
RP REVIEW.
RX PubMed=14690856; DOI=10.1080/08830180490265556;
RA Lichtenstein D.L., Toth K., Doronin K., Tollefson A.E., Wold W.S.;
RT "Functions and mechanisms of action of the adenovirus E3 proteins.";
RL Int. Rev. Immunol. 23:75-111(2004).
RN [9]
RP REVIEW.
RX PubMed=14674598; DOI=10.1007/978-3-662-05599-1_2;
RA Windheim M., Hilgendorf A., Burgert H.G.;
RT "Immune evasion by adenovirus E3 proteins: exploitation of intracellular
RT trafficking pathways.";
RL Curr. Top. Microbiol. Immunol. 273:29-85(2004).
CC -!- FUNCTION: Prevents infected cell apoptosis induced by the host immune
CC system. Acts by down-regulating a number of cell surface receptors in
CC the tumor necrosis factor (TNF) receptor superfamily, namely FAS,
CC TNFRSF10A/TRAIL receptor 1, and TNFRSF10B/TRAIL receptor 2. Down-
CC regulation of these death receptors protects adenovirus-infected cells
CC from apoptosis induced by the death receptor ligands Fas ligand and
CC TRAIL. RID complex also down-regulates certain tyrosine kinase cell
CC surface receptors, especially the epidermal growth factor receptor
CC (EGFR). RID-mediated Fas and EGFR down-regulation occurs via
CC endocytosis of the receptors into endosomes followed by transport to
CC and degradation within lysosomes.
CC -!- SUBUNIT: Interacts with E3 RID-alpha and E3 CR1-alpha.
CC -!- SUBCELLULAR LOCATION: Host membrane {ECO:0000269|PubMed:7983708};
CC Single-pass type I membrane protein {ECO:0000269|PubMed:7983708}.
CC -!- PTM: Phosphorylated on serine. {ECO:0000269|PubMed:1532104}.
CC -!- PTM: O-glycosylated, but not N-glycosylated.
CC {ECO:0000269|PubMed:1533979}.
CC -!- SIMILARITY: Belongs to the adenoviridae E3_RID-beta family.
CC {ECO:0000305}.
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DR EMBL; J01917; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; A03820; ERAD52.
DR RefSeq; AP_000187.1; AC_000007.1.
DR Proteomes; UP000008167; Genome.
DR GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009966; P:regulation of signal transduction; IEA:InterPro.
DR InterPro; IPR008131; Adeno_E3_14_5.
DR Pfam; PF04834; Adeno_E3_14_5; 1.
PE 1: Evidence at protein level;
KW Early protein; Glycoprotein; Host membrane; Membrane; Phosphoprotein;
KW Reference proteome; Signal; Transmembrane; Transmembrane helix.
FT SIGNAL 1..19
FT /evidence="ECO:0000250"
FT CHAIN 20..130
FT /note="Early 3 receptor internalization and degradation
FT beta protein"
FT /id="PRO_0000036476"
FT TRANSMEM 53..77
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 122..125
FT /note="Tyrosine-based sorting motif"
SQ SEQUENCE 130 AA; 14529 MW; 4FADC90F04ABE5D1 CRC64;
MKRSVIFVLL IFCALPVLCS QTSAPPKRHI SCRFTQIWNI PSCYNKQSDL SEAWLYAIIS
VMVFCSTIFA LAIYPYLDIG WNAIDAMNHP TFPVPAVIPL QQVIAPINQP RPPSPTPTEI
SYFNLTGGDD
